Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,292,182 G→T 7.4% G8G (GGC→GGA)  yraR ← putative nucleoside‑diphosphate‑sugar epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,292,1820GT 7.4% 1248.6 / 43.6 334G8G (GGC→GGA) yraRputative nucleoside‑diphosphate‑sugar epimerase
Reads supporting (aligned to +/- strand):  ref base G (168/141);  new base T (13/12);  total (181/153)
Fisher's exact test for biased strand distribution p-value = 8.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 353 total aligned reads displayed.

TGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGAAG  >  CP009273/3292033‑3292331
                                                                                                                                                     |                                                                                                                                                     
tGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTgcg                                                                                                                                                       <  1:169916/150‑1 (MQ=255)
    ccAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTg                                                                                                                                                   >  2:2454179/1‑150 (MQ=255)
      aaCGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTa                                                                                                                                                 >  1:1083545/1‑150 (MQ=255)
        cgcgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAAt                                                                                                                                               >  1:3072836/1‑150 (MQ=255)
         gcgTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATc                                                                                                                                              <  1:1336565/150‑1 (MQ=255)
           gTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAg                                                                                                                                            >  1:2049295/1‑150 (MQ=255)
              aCTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTAc                                                                                                                                         >  1:3456177/1‑150 (MQ=255)
               cTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTAcc                                                                                                                                        >  1:3512553/1‑150 (MQ=255)
                 cAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTg                                                                                                                                      <  2:1751475/150‑1 (MQ=255)
                  aGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGa                                                                                                                                     >  1:2166625/1‑150 (MQ=255)
                    ttGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACt                                                                                                                                   <  1:273946/150‑1 (MQ=255)
                      gCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCa                                                                                                                                 <  1:608053/150‑1 (MQ=255)
                      gCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCa                                                                                                                                 >  1:2556209/1‑150 (MQ=255)
                         gATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCt                                                                                                                              <  1:2787990/150‑1 (MQ=255)
                           tCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                                                                                            <  2:3691025/150‑1 (MQ=255)
                           tCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTc                                                                                                                            <  2:3854596/150‑1 (MQ=255)
                             aTGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGc                                                                                                                          >  2:3303629/1‑150 (MQ=255)
                               ggggATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTc                                                                                                                        >  2:2969445/1‑150 (MQ=255)
                                  gATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCtt                                                                                                                     <  2:2758937/150‑1 (MQ=255)
                                   aTTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                                                                                    >  1:4350997/1‑150 (MQ=255)
                                   aTTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCttt                                                                                                                    <  2:3420888/150‑1 (MQ=255)
                                     tAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTAc                                                                                                                  >  1:1530949/1‑150 (MQ=255)
                                         cacCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGaa                                                                                                              >  1:1823986/1‑150 (MQ=255)
                                          acCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAt                                                                                                             >  2:4469117/1‑150 (MQ=255)
                                           ccccGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAAtt                                                                                                            >  2:1975724/1‑150 (MQ=255)
                                                 gCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGc                                                                                                      <  2:2243019/150‑1 (MQ=255)
                                                  cATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGcc                                                                                                     >  2:2056426/1‑150 (MQ=255)
                                                  cATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGcc                                                                                                     >  2:613285/1‑150 (MQ=255)
                                                   atatCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCt                                                                                                    >  1:2188366/1‑150 (MQ=255)
                                                      tCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgc                                                                                                 >  2:1457090/1‑150 (MQ=255)
                                                       cGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcg                                                                                                >  2:1339597/1‑150 (MQ=255)
                                                        gCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTgcgc                                                                                               >  2:2613088/1‑150 (MQ=255)
                                                         cccAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCt                                                                                              <  2:255952/150‑1 (MQ=255)
                                                           cAACGGACGTCGCGTCGGCGCAGCATTGGCGTTAAATTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTAATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTcc                                                                                            <  2:1782458/150‑1 (MQ=255)
                                                           cAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTcc                                                                                            >  1:2095178/1‑150 (MQ=255)
                                                            aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                                                                                           >  1:306518/1‑150 (MQ=255)
                                                            aaCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCa                                                                                           >  1:3691102/1‑150 (MQ=255)
                                                             aCGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCAc                                                                                          >  2:4154569/1‑150 (MQ=255)
                                                              cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                                                                                         <  1:719919/150‑1 (MQ=255)
                                                              cGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACg                                                                                         >  2:3448663/1‑150 (MQ=255)
                                                               ggACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACgg                                                                                        >  2:3046520/1‑150 (MQ=255)
                                                                gACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGc                                                                                       <  2:3623303/150‑1 (MQ=255)
                                                                 aCGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCt                                                                                      <  1:869616/150‑1 (MQ=255)
                                                                  cgtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTc                                                                                     <  2:1049595/150‑1 (MQ=255)
                                                                   gtcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCa                                                                                    >  1:2032140/1‑150 (MQ=255)
                                                                    tcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                                                                                   <  2:4249381/150‑1 (MQ=255)
                                                                    tcgcgtcgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCAt                                                                                   <  2:3581172/150‑1 (MQ=255)
                                                                          cgGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCa                                                                             <  2:487409/150‑1 (MQ=255)
                                                                           ggCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAg                                                                            >  2:142614/1‑150 (MQ=255)
                                                                                  caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt                                                                     <  2:3704762/150‑1 (MQ=255)
                                                                                  caATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAt                                                                     <  1:1655954/150‑1 (MQ=255)
                                                                                    aTGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAg                                                                   <  2:2494522/150‑1 (MQ=255)
                                                                                      ggCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAc                                                                 >  1:4133066/1‑150 (MQ=255)
                                                                                       gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAcc                                                                >  2:139580/1‑150 (MQ=255)
                                                                                       gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAcc                                                                <  2:2797116/150‑1 (MQ=255)
                                                                                       gCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGAcc                                                                <  2:2797630/150‑1 (MQ=255)
                                                                                        cGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCa                                                               >  1:2661405/1‑150 (MQ=255)
                                                                                         gTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCAt                                                              <  2:11729/150‑1 (MQ=255)
                                                                                          ttAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATg                                                             <  2:1325836/150‑1 (MQ=255)
                                                                                            aaCTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAg                                                           <  2:3390644/150‑1 (MQ=255)
                                                                                             aCTTTCGGTTCGTTAATCCACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAgg                                                          >  2:2349660/1‑150 (MQ=255)
                                                                                              cTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGt                                                         >  1:3754404/1‑150 (MQ=255)
                                                                                                 tCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAAt                                                      >  1:4419491/1‑150 (MQ=255)
                                                                                                     ttCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATc                                                  <  2:116417/150‑1 (MQ=255)
                                                                                                     ttCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATc                                                  <  1:594785/150‑1 (MQ=255)
                                                                                                      tCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATcc                                                 >  2:2066101/1‑150 (MQ=255)
                                                                                                       cGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATccc                                                >  2:2152495/1‑150 (MQ=255)
                                                                                                        gTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATcccc                                               >  1:1364355/1‑150 (MQ=255)
                                                                                                          tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGa                                             <  2:3312727/150‑1 (MQ=255)
                                                                                                          tAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGa                                             <  2:3975721/150‑1 (MQ=255)
                                                                                                           aaTCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGAt                                            <  2:1610484/150‑1 (MQ=255)
                                                                                                            aTCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGAtt                                           >  1:2015670/1‑150 (MQ=255)
                                                                                                             tCAACATCCGCAGCAGGGGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTGGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATtg                                          <  2:2745251/150‑1 (MQ=255)
                                                                                                              cAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATtgt                                         >  1:2265772/1‑150 (MQ=255)
                                                                                                                 cATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTggg                                      <  2:733211/150‑1 (MQ=255)
                                                                                                                 cATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTggg                                      <  2:1294323/150‑1 (MQ=255)
                                                                                                                  aTCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCTCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGa                                     >  1:4164066/1‑150 (MQ=255)
                                                                                                                   tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGac                                    >  1:2969978/1‑149 (MQ=255)
                                                                                                                   tCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAAAAGACCATGAGGTAATTATCCCCGATTGTGGGaa                                    >  1:690315/1‑150 (MQ=255)
                                                                                                                      gcagcaGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAAtt                                 <  2:4341517/150‑1 (MQ=255)
                                                                                                                       cagcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTc                                <  2:1043249/150‑1 (MQ=255)
                                                                                                                        agcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCg                               >  2:3314471/1‑150 (MQ=255)
                                                                                                                        agcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCg                               >  2:2192752/1‑150 (MQ=255)
                                                                                                                         gcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGc                              >  1:3820896/1‑150 (MQ=255)
                                                                                                                         gcagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGc                              >  1:1561970/1‑150 (MQ=255)
                                                                                                                          cagGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGcc                             >  1:1886171/1‑150 (MQ=255)
                                                                                                                           agGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCt                            >  1:4207586/1‑150 (MQ=255)
                                                                                                                           agGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCt                            <  2:4028911/150‑1 (MQ=255)
                                                                                                                                   gCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAAt                    >  1:138011/1‑150 (MQ=255)
                                                                                                                                     ccaccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGc                  >  1:807926/1‑150 (MQ=255)
                                                                                                                                      caccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGca                 <  1:3922811/150‑1 (MQ=255)
                                                                                                                                      caccaGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGca                 <  2:4011808/150‑1 (MQ=255)
                                                                                                                                         caGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGTAAATTCGCCTCATCCAATGcaaca              >  1:1924435/1‑150 (MQ=255)
                                                                                                                                           gCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCaacaac            <  1:1262321/150‑1 (MQ=255)
                                                                                                                                           gCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCaacaac            <  1:1189765/150‑1 (MQ=255)
                                                                                                                                                gTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCgga       <  1:2510063/150‑1 (MQ=255)
                                                                                                                                                 gtGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCggag      <  1:2454165/149‑1 (MQ=255)
                                                                                                                                                   gcgcCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCggagga    <  1:4256719/150‑1 (MQ=255)
                                                                                                                                                   gcgcCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCggagga    <  2:306518/150‑1 (MQ=255)
                                                                                                                                                     gcCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGAAg  <  1:630460/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCGCCAACGCGTCACTCAGTTGCGGATCATGGGGATTAAACACCCCGGGCATATCGCCCAACGGACGTCGCGTCGGCGCAGCAATGGCGTTAACTTTCGGTTCGTTAATCAACATCCGCAGCAGGTGACCGCCCACCAGCCCCGTTGCGCCTGTAATCAGTACCTGACTCATCTTCGCTCCTTTACAGAATTGTCTGCCTTGCGCTCCACGGCTCATGCACCAGGCTTAATAGACCATGAGGTAATTATCCCCGATTGTGGGAAATTCGCCTCATCCAATGCAACAACGCGGAGGAAG  >  CP009273/3292033‑3292331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑