Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,387,166 C→A 5.8% V818F (GTT→TTT)  yhdP ← DUF3971‑AsmA2 domains protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,387,1660CA 5.8% 994.1 / 23.4 263V818F (GTT→TTT) yhdPDUF3971‑AsmA2 domains protein
Reads supporting (aligned to +/- strand):  ref base C (116/131);  new base A (9/7);  total (125/138)
Fisher's exact test for biased strand distribution p-value = 6.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

GGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCC  >  CP009273/3387018‑3387311
                                                                                                                                                    |                                                                                                                                                 
 gCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGt                                                                                                                                                 <  2:3232574/150‑1 (MQ=255)
   aaCGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGttt                                                                                                                                               >  2:2731825/1‑150 (MQ=255)
      ggcggGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTAcc                                                                                                                                            <  2:4501224/150‑1 (MQ=255)
      ggcggGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTAcc                                                                                                                                            <  2:4502939/150‑1 (MQ=255)
      ggcggGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTAcc                                                                                                                                            >  1:49297/1‑150 (MQ=255)
      ggcggGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTAcc                                                                                                                                            >  1:3320380/1‑150 (MQ=255)
           gAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAg                                                                                                                                       >  1:1297473/1‑150 (MQ=255)
           gAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAg                                                                                                                                       >  1:1033191/1‑150 (MQ=255)
            aGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGt                                                                                                                                      <  1:3650651/150‑1 (MQ=255)
                ttttACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGtt                                                                                                                                  <  1:533429/150‑1 (MQ=255)
                 tttACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTc                                                                                                                                 <  1:304146/150‑1 (MQ=255)
                   tACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTCGTGGTTCAc                                                                                                                               >  2:4104361/1‑150 (MQ=255)
                   tACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCAc                                                                                                                               >  2:797987/1‑150 (MQ=255)
                     cTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCAccc                                                                                                                             >  2:2080993/1‑150 (MQ=255)
                      tGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCg                                                                                                                            >  1:2793946/1‑150 (MQ=255)
                      tGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCg                                                                                                                            <  1:3818139/150‑1 (MQ=255)
                        tCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCa                                                                                                                          <  1:2998225/150‑1 (MQ=255)
                        tCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCa                                                                                                                          <  1:3091846/150‑1 (MQ=255)
                            ccgccCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGtt                                                                                                                      >  1:842999/1‑150 (MQ=255)
                             cgccCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGttt                                                                                                                     >  1:1873472/1‑150 (MQ=255)
                               cccAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTgg                                                                                                                   >  2:2869207/1‑150 (MQ=255)
                               cccAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTgg                                                                                                                   >  2:87750/1‑150 (MQ=255)
                                 cAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCt                                                                                                                 >  2:1426942/1‑150 (MQ=255)
                                 cAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCt                                                                                                                 <  2:1163060/150‑1 (MQ=255)
                                 cAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCt                                                                                                                 >  2:3691862/1‑150 (MQ=255)
                                  aaaTAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGAGGTTCACCCGCAGGTTTGGCTa                                                                                                                >  2:2665378/1‑150 (MQ=255)
                                   aaTAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTaa                                                                                                               <  1:2508091/150‑1 (MQ=255)
                                   aaTAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATCCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTaa                                                                                                               <  1:3958464/150‑1 (MQ=255)
                                     tAGCACGATCGAGCGTCAGCTTTTGACCGAGCAGCCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCGAGTGGTTCACCCGCAGGTTTGGCTAACg                                                                                                             >  1:3681995/1‑150 (MQ=255)
                                     tAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACg                                                                                                             <  1:1292351/150‑1 (MQ=255)
                                          cGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAATCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGaa                                                                                                        <  1:25525/150‑1 (MQ=255)
                                          cGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGaa                                                                                                        >  1:955746/1‑150 (MQ=255)
                                          cGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGaa                                                                                                        <  1:2732014/150‑1 (MQ=255)
                                            aTCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAgg                                                                                                      >  1:4186911/1‑150 (MQ=255)
                                            aTCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAgg                                                                                                      <  2:4127519/150‑1 (MQ=255)
                                               gAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTaa                                                                                                   >  2:568764/1‑150 (MQ=255)
                                               gAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTaa                                                                                                   >  2:3659629/1‑150 (MQ=255)
                                                  cGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTg                                                                                                <  1:533870/150‑1 (MQ=255)
                                                    tCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGAc                                                                                              >  2:3614675/1‑150 (MQ=255)
                                                     cAGCTTTTGACCGAGCAACCAGCGGCTATTGACATGTTTATCCGCTCCAGCATTTCGGGTTTATTCTAAGCTGTTGTGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTTAGTGACt                                                                                             <  2:484883/150‑1 (MQ=255)
                                                           ttGACCGAGCAACCAGCGGCTATTGGAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTcac                                                                                       >  2:14620/1‑150 (MQ=255)
                                                           ttGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAATTGACTGATcac                                                                                       >  2:152872/1‑150 (MQ=255)
                                                           ttGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTcac                                                                                       >  2:2318045/1‑150 (MQ=255)
                                                                 gAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCtt                                                                                 <  2:3973864/150‑1 (MQ=255)
                                                                 gAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCtt                                                                                 <  1:1210908/150‑1 (MQ=255)
                                                                  aGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCttt                                                                                >  1:2282546/1‑150 (MQ=255)
                                                                   gCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTa                                                                               >  2:478477/1‑150 (MQ=255)
                                                                    cAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGATAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAg                                                                              >  2:1785007/1‑150 (MQ=255)
                                                                    cAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAg                                                                              >  1:1140290/1‑150 (MQ=255)
                                                                       ccAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATc                                                                           <  2:1188778/150‑1 (MQ=255)
                                                                       ccAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATc                                                                           <  2:1188830/150‑1 (MQ=255)
                                                                        cAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCg                                                                          <  1:693469/150‑1 (MQ=255)
                                                                        cAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCg                                                                          >  2:4456277/1‑150 (MQ=255)
                                                                          gCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGcc                                                                        <  1:224105/150‑1 (MQ=255)
                                                                             gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                     <  2:2631898/150‑1 (MQ=255)
                                                                             gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                     <  1:1345769/150‑1 (MQ=255)
                                                                             gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                     <  1:3558463/150‑1 (MQ=255)
                                                                             gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                     <  1:2390111/150‑1 (MQ=255)
                                                                             gCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGtt                                                                     <  1:2390148/150‑1 (MQ=255)
                                                                              cTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTc                                                                    >  1:3289424/1‑150 (MQ=255)
                                                                              cTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTc                                                                    >  1:4391127/1‑150 (MQ=255)
                                                                               tATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCa                                                                   >  1:2979954/1‑150 (MQ=255)
                                                                                aTTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAg                                                                  <  1:4116923/150‑1 (MQ=255)
                                                                                        gATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGt                                                          <  2:2208448/150‑1 (MQ=255)
                                                                                            aTCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTtata                                                      <  2:3857747/150‑1 (MQ=255)
                                                                                             tCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAg                                                     >  2:620196/1‑150 (MQ=255)
                                                                                                gCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTc                                                  <  2:867786/150‑1 (MQ=255)
                                                                                                gCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTc                                                  >  1:4253508/1‑150 (MQ=255)
                                                                                                 cACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCg                                                 >  1:3459264/1‑150 (MQ=255)
                                                                                                    cAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCAc                                              >  1:819738/1‑150 (MQ=255)
                                                                                                       ccTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAg                                           <  2:910002/150‑1 (MQ=255)
                                                                                                        cTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTCACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGc                                          >  2:2765075/1‑150 (MQ=255)
                                                                                                          gTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCAt                                        <  2:2826389/150‑1 (MQ=255)
                                                                                                          gTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCAt                                        <  2:2196752/150‑1 (MQ=255)
                                                                                                              ggTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATa                                    >  1:3417874/1‑150 (MQ=255)
                                                                                                               gTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATaa                                   <  1:1720886/150‑1 (MQ=255)
                                                                                                                 tAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAgg                                 <  2:3853682/150‑1 (MQ=255)
                                                                                                                   aTTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCa                               <  1:1616763/150‑1 (MQ=255)
                                                                                                                     tCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGa                             <  2:1964390/150‑1 (MQ=255)
                                                                                                                       aaaGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATc                           <  2:1845502/150‑1 (MQ=255)
                                                                                                                        aaGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCa                          <  1:140246/150‑1 (MQ=255)
                                                                                                                        aaGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCa                          >  2:4329112/1‑150 (MQ=255)
                                                                                                                         aGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCaa                         >  1:3228440/1‑150 (MQ=255)
                                                                                                                               tGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                   >  2:1082293/1‑150 (MQ=255)
                                                                                                                               tGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCa                   >  2:1630398/1‑150 (MQ=255)
                                                                                                                                gagaTTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCAc                  <  1:3429545/150‑1 (MQ=255)
                                                                                                                                 agaTTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACt                 <  1:2382970/150‑1 (MQ=255)
                                                                                                                                   aTTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACttt               >  2:1036141/1‑150 (MQ=255)
                                                                                                                                   aTTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACttt               <  2:3408986/150‑1 (MQ=255)
                                                                                                                                    ttGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTa              <  2:2980601/150‑1 (MQ=255)
                                                                                                                                      gCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTAcc            >  2:2470950/1‑150 (MQ=255)
                                                                                                                                      gCCATCAACCTTAAAGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTGTAcc            >  2:4132208/1‑150 (MQ=255)
                                                                                                                                       ccATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCa           <  1:2603089/150‑1 (MQ=255)
                                                                                                                                         aTCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATc         >  2:2238533/1‑150 (MQ=255)
                                                                                                                                          tCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATcc        <  2:1514178/150‑1 (MQ=255)
                                                                                                                                            aaCCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCcca      >  1:2413064/1‑150 (MQ=255)
                                                                                                                                            aaCCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCcca      <  1:2080993/150‑1 (MQ=255)
                                                                                                                                             aCCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccac     >  1:3750438/1‑150 (MQ=255)
                                                                                                                                               cTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgc   >  2:2562870/1‑150 (MQ=255)
                                                                                                                                                ttAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCccacgcc  <  2:4528827/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGCAACGGCGGGAGCGTTTTACTGTCTGCCGCCCAAATAGCACGATCGAGCGTCAGCTTTTGACCGAGCAACCAGCGGCTATTGAAATGATTATCCGCACCAGCCTGTCCGGTTAATTCAAAGCTGTTGAGATTGCCATCAACCTTAACGTTTACCGCTAGTGGTTCACCCGCAGGTTTGGCTAACGGTGAAGGTAAGTGACTGCTCACATTCTTTAGATCGCCGTTCAGCTCTATGTTATAGGTCGCACCAGCATGATAAGGCAGATCAATGCCCACTTTACCATCCCACGCC  >  CP009273/3387018‑3387311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑