Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 421,799 C→A 100% I69I (ATC→ATA)  tgt → tRNA‑guanine transglycosylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273421,7990CA96.5% 846.5 / 19.5 255I69I (ATC→ATA) tgttRNA‑guanine transglycosylase
Reads supporting (aligned to +/- strand):  ref base C (3/6);  new base A (132/114);  total (135/120)
Fisher's exact test for biased strand distribution p-value = 3.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 264 total aligned reads displayed.

TCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGA  >  CP009273/421652‑421945
                                                                                                                                                   |                                                                                                                                                  
tCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAaataat                                                                                                                                                  <  2:53352/150‑1 (MQ=255)
 cGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATg                                                                                                                                                 <  1:359421/150‑1 (MQ=255)
 cGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATg                                                                                                                                                 <  2:238092/150‑1 (MQ=255)
  gTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGa                                                                                                                                                >  1:3739621/1‑150 (MQ=255)
      cGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACt                                                                                                                                            <  2:4055352/150‑1 (MQ=255)
       gTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGTCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTg                                                                                                                                           <  1:1609929/150‑1 (MQ=255)
           tGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACg                                                                                                                                       <  2:3478420/150‑1 (MQ=255)
           tGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACg                                                                                                                                       <  2:216044/150‑1 (MQ=255)
            ggAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACgg                                                                                                                                      >  2:337429/1‑150 (MQ=255)
                  gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                                >  2:674828/1‑150 (MQ=255)
                  gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                                <  2:1483219/150‑1 (MQ=255)
                  gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                                >  1:2953256/1‑150 (MQ=255)
                  gCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCt                                                                                                                                <  2:1928659/150‑1 (MQ=255)
                     ttGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCa                                                                                                                             <  1:79063/150‑1 (MQ=255)
                     ttGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCa                                                                                                                             <  1:2167486/150‑1 (MQ=255)
                      tGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCAc                                                                                                                            <  1:1053426/150‑1 (MQ=255)
                       gTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACg                                                                                                                           <  1:3495189/150‑1 (MQ=255)
                       gTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACg                                                                                                                           <  2:3762596/150‑1 (MQ=255)
                                 tGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCa                                                                                                                 >  1:904608/1‑150 (MQ=255)
                                     ggCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTgg                                                                                                             >  2:366909/1‑150 (MQ=255)
                                       cACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGaa                                                                                                           <  2:2437207/150‑1 (MQ=255)
                                         ccTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAgg                                                                                                         >  1:3800546/1‑150 (MQ=255)
                                           tACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAgggg                                                                                                       >  1:753368/1‑150 (MQ=255)
                                            aCGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGc                                                                                                      >  2:1863006/1‑150 (MQ=255)
                                             cGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGcc                                                                                                     >  2:1548015/1‑150 (MQ=255)
                                             cGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGcc                                                                                                     >  2:1055461/1‑150 (MQ=255)
                                               gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGa                                                                                                   <  1:2569832/150‑1 (MQ=255)
                                               gCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGa                                                                                                   >  1:4137703/1‑150 (MQ=255)
                                                cACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGAt                                                                                                  <  2:2400028/150‑1 (MQ=255)
                                                 aCCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATc                                                                                                 <  2:3525716/150‑1 (MQ=255)
                                                 aCCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATc                                                                                                 <  1:587543/150‑1 (MQ=255)
                                                     tAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCa                                                                                             >  1:1584008/1‑150 (MQ=255)
                                                      aaaaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCAc                                                                                            >  1:970085/1‑150 (MQ=255)
                                                        aaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCg                                                                                          <  1:1449561/150‑1 (MQ=255)
                                                        aaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCg                                                                                          <  2:887652/150‑1 (MQ=255)
                                                        aaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCg                                                                                          >  2:2318885/1‑150 (MQ=255)
                                                        aaGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGAACCTCACCg                                                                                          <  1:3753948/150‑1 (MQ=255)
                                                             aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTcc                                                                                     <  1:616845/150‑1 (MQ=255)
                                                             aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTcc                                                                                     <  1:435860/150‑1 (MQ=255)
                                                             aTGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTcc                                                                                     >  2:2717708/1‑150 (MQ=255)
                                                               gACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcgg                                                                                   <  1:273102/150‑1 (MQ=255)
                                                                aCGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCcggc                                                                                  <  2:907599/150‑1 (MQ=255)
                                                                    cGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCt                                                                              <  1:3818591/150‑1 (MQ=255)
                                                                     ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                             >  1:3930952/1‑150 (MQ=255)
                                                                     ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                             >  2:864651/1‑150 (MQ=255)
                                                                     ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                             >  1:1695346/1‑150 (MQ=255)
                                                                     ggAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCtt                                                                             >  1:2927111/1‑150 (MQ=255)
                                                                       aagaagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAAAAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGAGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTcc                                                                           >  1:2294082/1‑150 (MQ=255)
                                                                          aagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAgg                                                                        >  1:3841641/1‑150 (MQ=255)
                                                                          aagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAgg                                                                        <  1:522316/150‑1 (MQ=255)
                                                                          aagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAgg                                                                        >  1:1931082/1‑150 (MQ=255)
                                                                          aagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAgg                                                                        >  2:1987610/1‑150 (MQ=255)
                                                                          aagTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAgg                                                                        <  2:1597249/150‑1 (MQ=255)
                                                                           agTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGt                                                                       >  1:1099000/1‑150 (MQ=255)
                                                                             ttGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCt                                                                     >  2:2685671/1‑150 (MQ=255)
                                                                                aaGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCa                                                                  <  2:2336258/150‑1 (MQ=255)
                                                                                aaGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCa                                                                  <  2:1737394/150‑1 (MQ=255)
                                                                                aaGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCa                                                                  >  1:78198/1‑150 (MQ=255)
                                                                                 aGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAg                                                                 >  1:1848836/1‑150 (MQ=255)
                                                                                 aGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAg                                                                 <  2:3143483/150‑1 (MQ=255)
                                                                                   ccACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGcc                                                               <  2:2939476/150‑1 (MQ=255)
                                                                                     aCTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCtt                                                             <  2:223627/150‑1 (MQ=255)
                                                                                      cTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTg                                                            <  1:2883755/150‑1 (MQ=255)
                                                                                      cTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTg                                                            <  1:2144121/150‑1 (MQ=255)
                                                                                      cTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTg                                                            >  2:2119340/1‑150 (MQ=255)
                                                                                       tGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTgg                                                           <  1:2675828/150‑1 (MQ=255)
                                                                                         gcgcgcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCg                                                         <  2:1945331/150‑1 (MQ=255)
                                                                                            cgcAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGata                                                      >  1:2017410/1‑150 (MQ=255)
                                                                                              cAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATAtt                                                    >  1:3083545/1‑150 (MQ=255)
                                                                                              cAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATAtt                                                    >  1:3083147/1‑150 (MQ=255)
                                                                                               aaaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTc                                                   >  2:2826757/1‑150 (MQ=255)
                                                                                               aaaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTc                                                   <  1:2516540/150‑1 (MQ=255)
                                                                                                aaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCg                                                  >  2:716654/1‑150 (MQ=255)
                                                                                                aaTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCg                                                  <  2:3103974/150‑1 (MQ=255)
                                                                                                 aTTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGt                                                 <  2:2597728/150‑1 (MQ=255)
                                                                                                            cAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGa                                      >  2:628274/1‑150 (MQ=255)
                                                                                                               cacCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACa                                   <  2:1319194/150‑1 (MQ=255)
                                                                                                                   ttCCACCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGc                               >  1:2067400/1‑150 (MQ=255)
                                                                                                                       aCCTGTGGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGc                           >  1:41553/1‑150 (MQ=255)
                                                                                                                          tgtgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACt                        >  2:3419366/1‑150 (MQ=255)
                                                                                                                            tgGCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTc                      >  1:176134/1‑150 (MQ=255)
                                                                                                                             ggCTGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTcc                     >  2:4137972/1‑150 (MQ=255)
                                                                                                                                tGCGCCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCTCGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTa                  <  1:3352695/150‑1 (MQ=255)
                                                                                                                                  cgcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAc                <  1:3176288/150‑1 (MQ=255)
                                                                                                                                   gcCCGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAAcc               >  1:3435367/1‑150 (MQ=255)
                                                                                                                                     ccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCg             >  1:4031737/1‑150 (MQ=255)
                                                                                                                                     ccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCg             >  1:1403209/1‑150 (MQ=255)
                                                                                                                                     ccGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCg             <  1:2807473/150‑1 (MQ=255)
                                                                                                                                      cGGGCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGa            >  2:4091667/1‑150 (MQ=255)
                                                                                                                                        ggCCAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATc          <  1:2587642/150‑1 (MQ=255)
                                                                                                                                          ccAGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCaa        <  1:2035731/150‑1 (MQ=255)
                                                                                                                                            aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACg      >  1:1280052/1‑150 (MQ=255)
                                                                                                                                            aGGAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCATCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACg      >  1:496908/1‑150 (MQ=255)
                                                                                                                                             ggAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACgg     >  2:516927/1‑150 (MQ=255)
                                                                                                                                             ggAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACgg     >  2:3055517/1‑150 (MQ=255)
                                                                                                                                             ggAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACgg     <  1:2678804/150‑1 (MQ=255)
                                                                                                                                              gAAATAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGc    <  2:1061034/150‑1 (MQ=255)
                                                                                                                                               aaaTAATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCg   <  1:3385074/150‑1 (MQ=255)
                                                                                                                                                aataatGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGa  >  1:3334720/1‑150 (MQ=255)
                                                                                                                                                aataatGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGa  >  2:4174223/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGTGGCGTAGTGGAAACGCCTTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTATCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATGCAGTGGAAGGGGCCGATCCTCACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGCGATATTCGTAAAATCACCGAACAGGGCGTGCACTTCCGTAACCCGATCAACGGCGA  >  CP009273/421652‑421945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑