Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,207,558 G→T 37.6% A174A (GCG→GCT)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,207,5580GT37.6% 316.8 / 226.8 220A174A (GCG→GCT) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  ref base G (73/64);  new base T (36/46);  total (110/110)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 233 total aligned reads displayed.

ACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGG  >  CP009273/4207410‑4207706
                                                                                                                                                    |                                                                                                                                                    
acaacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCga                                                                                                                                                     >  1:1230387/1‑150 (MQ=255)
 caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgat                                                                                                                                                    <  1:281691/150‑1 (MQ=255)
 caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgat                                                                                                                                                    >  2:587743/1‑150 (MQ=255)
 caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCtat                                                                                                                                                    <  1:262025/150‑1 (MQ=255)
 caacaCCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCtat                                                                                                                                                    >  1:1701596/1‑150 (MQ=255)
 caacaCCTTCCGTAGTGCCGATCAGATCCAATGGTCCTCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGTAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCtat                                                                                                                                                    <  2:1642400/150‑1 (MQ=255)
   acacCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCgatga                                                                                                                                                  >  2:697431/1‑150 (MQ=255)
     cccTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGAtt                                                                                                                                                <  2:1477131/149‑1 (MQ=255)
     acCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGAtt                                                                                                                                                >  1:3129049/1‑150 (MQ=255)
      ccTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTg                                                                                                                                               >  2:3707288/1‑150 (MQ=255)
         tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAg                                                                                                                                            >  1:1695115/1‑150 (MQ=255)
         tCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAg                                                                                                                                            <  2:652226/150‑1 (MQ=255)
          ccGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGc                                                                                                                                           <  2:1618518/150‑1 (MQ=255)
               gtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTg                                                                                                                                      <  2:3462621/150‑1 (MQ=255)
               gtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTg                                                                                                                                      <  2:3875167/150‑1 (MQ=255)
               gtGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTg                                                                                                                                      <  2:1369302/150‑1 (MQ=255)
                 gCCGATCAGTTCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCa                                                                                                                                    <  1:2782360/150‑1 (MQ=255)
                 gCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCa                                                                                                                                    <  2:106541/150‑1 (MQ=255)
                  ccGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAg                                                                                                                                   <  1:288301/150‑1 (MQ=255)
                  ccGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAg                                                                                                                                   <  1:1179268/150‑1 (MQ=255)
                    gATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCg                                                                                                                                 <  2:1022716/150‑1 (MQ=255)
                    gATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCg                                                                                                                                 >  2:2085245/1‑150 (MQ=255)
                     aTCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCgg                                                                                                                                <  2:937259/150‑1 (MQ=255)
                         gATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGt                                                                                                                            <  2:3949195/150‑1 (MQ=255)
                           tCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTc                                                                                                                          <  2:1144449/150‑1 (MQ=255)
                               aTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACtt                                                                                                                      <  1:1537079/150‑1 (MQ=255)
                               aTGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACtt                                                                                                                      >  1:68463/1‑150 (MQ=255)
                                tGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTc                                                                                                                     <  1:2459125/150‑1 (MQ=255)
                                 ggTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCg                                                                                                                    <  2:3035256/150‑1 (MQ=255)
                                      gcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGAt                                                                                                               <  2:1835235/150‑1 (MQ=255)
                                      gcgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGAt                                                                                                               <  1:561524/150‑1 (MQ=255)
                                       cgGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGACTGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATc                                                                                                              <  1:1781935/150‑1 (MQ=255)
                                        gggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCa                                                                                                             >  2:228000/1‑150 (MQ=255)
                                        gggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCa                                                                                                             <  2:1605205/150‑1 (MQ=255)
                                        gggCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCa                                                                                                             >  2:1006295/1‑150 (MQ=255)
                                           cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCt                                                                                                          <  1:1496745/150‑1 (MQ=255)
                                           cATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCt                                                                                                          <  1:2756106/150‑1 (MQ=255)
                                            aTTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTg                                                                                                         <  2:829031/150‑1 (MQ=255)
                                             ttGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTgg                                                                                                        <  1:946497/150‑1 (MQ=255)
                                              tGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGc                                                                                                       >  1:1203006/1‑150 (MQ=255)
                                                aGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGt                                                                                                     >  1:4056293/1‑150 (MQ=255)
                                                    gggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGt                                                                                                 >  2:2928451/1‑150 (MQ=255)
                                                    gggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGt                                                                                                 >  1:3320388/1‑150 (MQ=255)
                                                     ggCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTg                                                                                                <  2:667597/150‑1 (MQ=255)
                                                       cGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaa                                                                                              >  1:3353769/1‑150 (MQ=255)
                                                       cGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaa                                                                                              <  1:2545141/150‑1 (MQ=255)
                                                        gATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaag                                                                                             >  2:2013991/1‑150 (MQ=255)
                                                        caacagcgcTATATCGAGAACTTCCTGACGATCTTTGCCGATGCGGAAGCCGGTATTGGCGATGTCCTGAATGCCATTGAACTGAAGAAAGAAATGATAGAAGCCCGTTCAGCGGCAGTTCACTTCGAAGATCACCTGGCTTCAGTgaag                                                                                             >  2:107009/6‑150 (MQ=255)
                                                         aTCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTgaaga                                                                                            <  2:2600796/150‑1 (MQ=255)
                                                             gcgcTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATg                                                                                        <  2:3904971/150‑1 (MQ=255)
                                                                cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCgg                                                                                     >  1:774312/1‑150 (MQ=255)
                                                                cTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCgg                                                                                     >  1:261929/1‑150 (MQ=255)
                                                                   tGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTca                                                                                  >  1:1038539/1‑150 (MQ=255)
                                                                              tCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCa                                                                       <  2:598351/150‑1 (MQ=255)
                                                                                cTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCaaa                                                                     <  2:4168547/150‑1 (MQ=255)
                                                                                 tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAg                                                                    <  2:2466225/150‑1 (MQ=255)
                                                                                 tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAg                                                                    <  2:2069529/150‑1 (MQ=255)
                                                                                 tGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAg                                                                    <  2:2383051/150‑1 (MQ=255)
                                                                                    cGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGttt                                                                 >  1:3248092/1‑150 (MQ=255)
                                                                                     gATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGtttt                                                                >  1:3894359/1‑150 (MQ=255)
                                                                                      aTCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTa                                                               <  2:1528851/150‑1 (MQ=255)
                                                                                      aTCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTa                                                               <  2:3775445/150‑1 (MQ=255)
                                                                                        cGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGt                                                             >  2:2625067/1‑150 (MQ=255)
                                                                                           tGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGcc                                                          >  2:25488/1‑150 (MQ=255)
                                                                                           tGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGcc                                                          >  1:2675530/1‑150 (MQ=255)
                                                                                              cGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAAc                                                       >  2:2901842/1‑150 (MQ=255)
                                                                                                aTGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTc                                                     >  2:216356/1‑150 (MQ=255)
                                                                                                  gCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAg                                                   <  1:1993463/150‑1 (MQ=255)
                                                                                                   cGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAgg                                                  >  2:729528/1‑150 (MQ=255)
                                                                                                   cGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAgg                                                  >  2:414969/1‑150 (MQ=255)
                                                                                                     gAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGaa                                                >  2:1650031/1‑150 (MQ=255)
                                                                                                       aGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGc                                              >  2:930827/1‑150 (MQ=255)
                                                                                                        gCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCt                                             <  1:827606/150‑1 (MQ=255)
                                                                                                          cGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAt                                           >  2:252420/1‑150 (MQ=255)
                                                                                                           ggTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                          <  2:341163/150‑1 (MQ=255)
                                                                                                           ggTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                          <  2:214060/150‑1 (MQ=255)
                                                                                                           ggTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTAtt                                          <  2:1634092/150‑1 (MQ=255)
                                                                                                            gTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTc                                         >  2:2789720/1‑150 (MQ=255)
                                                                                                             ttttGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCa                                        <  2:3110372/150‑1 (MQ=255)
                                                                                                                tGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTTGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGaa                                     <  2:2114824/150‑1 (MQ=255)
                                                                                                                   cGGTGTCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACt                                  >  1:525433/1‑150 (MQ=255)
                                                                                                                     gtgtCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTgg                                >  1:2359304/1‑150 (MQ=255)
                                                                                                                      tgtCCTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGt                               <  1:3143303/150‑1 (MQ=255)
                                                                                                                          cTGAATGCCTTTGAACTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgcg                           <  1:2256921/150‑1 (MQ=255)
                                                                                                                          cTGAATGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTcgcg                           <  2:2642782/150‑1 (MQ=255)
                                                                                                                               tGCCTTTGAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTCTTCAGAAACTGGTCGCGGcgcg                      >  2:4130426/1‑150 (MQ=255)
                                                                                                                                      gAACTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTTAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCa               <  1:648593/150‑1 (MQ=255)
                                                                                                                                       taCTGATGAAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAg              >  2:1052201/2‑150 (MQ=255)
                                                                                                                                       aaCTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGAGAAGAAATGCGGTCACATGTGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAg              >  1:3865793/1‑150 (MQ=255)
                                                                                                                                        aCTGATTAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGc             >  1:1263863/1‑150 (MQ=255)
                                                                                                                                           gattaAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtga          <  1:3420218/150‑1 (MQ=255)
                                                                                                                                            attaaaGCGATGATTGAAGCCGTTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGAGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGTTCGCGGCGCGTCTGGCAGCtgac         <  1:1237076/147‑1 (MQ=255)
                                                                                                                                            atgaAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgac         >  1:2871711/1‑150 (MQ=255)
                                                                                                                                            atgaAAGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgac         <  2:3228039/150‑1 (MQ=255)
                                                                                                                                             ttaaaGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgacg        >  2:2271478/3‑150 (MQ=255)
                                                                                                                                               aaaGCTATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgacgtg      >  2:3548421/1‑150 (MQ=255)
                                                                                                                                               aaaGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgacgtg      <  1:1862176/150‑1 (MQ=255)
                                                                                                                                                 aGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCtgacgtgac    >  2:3533210/1‑150 (MQ=255)
                                                                                                                                                   cGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACgg  <  1:114801/150‑1 (MQ=255)
                                                                                                                                                   cGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACgg  >  1:164465/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACAACACCTTCCGTCGTGCCGATCAGATCCAATGGTCCGCGGGCATTGAGCCGGGCGATCCGCGCTATGTCGATTACTTCCTGCCGATCGTTGCCGATGCGGAAGCCGGTTTTGGCGGTGTCCTGAATGCCTTTGAACTGATGAAAGCGATGATTGAAGCCGGTGCAGCGGCAGTTCACTTCGAAGATCAGCTGGCGTCAGTGAAGAAATGCGGTCACATGGGCGGCAAAGTTTTAGTGCCAACTCAGGAAGCTATTCAGAAACTGGTCGCGGCGCGTCTGGCAGCTGACGTGACGG  >  CP009273/4207410‑4207706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑