Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,285,213 G→T 41.6% D269Y (GAT→TAT)  gltP → glutamate/aspartate:proton symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,285,2130GT41.6% 299.3 / inf 271D269Y (GAT→TAT) gltPglutamate/aspartate:proton symporter
Reads supporting (aligned to +/- strand):  ref base G (87/71);  new base T (50/63);  total (137/134)
Fisher's exact test for biased strand distribution p-value = 8.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 272 total aligned reads displayed.

TGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCT  >  CP009273/4285064‑4285361
                                                                                                                                                     |                                                                                                                                                    
  gCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGAt                                                                                                                                                    >  1:895429/1‑150 (MQ=255)
        tttGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTg                                                                                                                                              <  2:3674517/150‑1 (MQ=255)
            gTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTc                                                                                                                                          >  1:3942005/1‑150 (MQ=255)
               tctcGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTgg                                                                                                                                       >  1:3681660/1‑150 (MQ=255)
               tctcGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTATGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTgg                                                                                                                                       <  2:3707712/150‑1 (MQ=255)
                 tcGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTTATTCGTATTCTGAAAGATGAGCTGATTCTGGCg                                                                                                                                     >  2:1235295/1‑150 (MQ=255)
                 tcGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCg                                                                                                                                     <  2:2753747/150‑1 (MQ=255)
                     ctctGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACt                                                                                                                                 >  1:121830/1‑150 (MQ=255)
                     ctctGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACt                                                                                                                                 >  1:1652554/1‑150 (MQ=255)
                          tgGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTTTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACt                                                                                                                            <  2:3811951/150‑1 (MQ=255)
                             ccACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGcc                                                                                                                         >  1:2966124/1‑150 (MQ=255)
                              cACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCa                                                                                                                        <  1:3229650/150‑1 (MQ=255)
                              cACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCa                                                                                                                        <  2:1999084/150‑1 (MQ=255)
                              cACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCa                                                                                                                        <  2:271270/150‑1 (MQ=255)
                               aCTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAg                                                                                                                       >  2:3083964/1‑150 (MQ=255)
                                    cGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTg                                                                                                                  >  2:3611377/1‑150 (MQ=255)
                                      aaaCTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGACTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGaa                                                                                                                >  1:1977402/1‑150 (MQ=255)
                                       aaCTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGaaa                                                                                                               >  1:1329075/1‑150 (MQ=255)
                                        aCTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAg                                                                                                              <  1:471265/150‑1 (MQ=255)
                                         cTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGc                                                                                                             <  2:3781188/150‑1 (MQ=255)
                                          tGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCg                                                                                                            >  1:3604905/1‑150 (MQ=255)
                                           ggTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGt                                                                                                           >  2:228579/1‑150 (MQ=255)
                                                   tgGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGACTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCcgc                                                                                                   >  1:1855822/1‑150 (MQ=255)
                                                   tgGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCcgc                                                                                                   >  1:2613994/1‑150 (MQ=255)
                                                   tgGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCcgc                                                                                                   >  1:3476330/1‑150 (MQ=255)
                                                    ggTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCcgcg                                                                                                  <  1:1553996/150‑1 (MQ=255)
                                                        cATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAatt                                                                                              >  1:2424223/1‑150 (MQ=255)
                                                               ccATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTgaga                                                                                       >  1:2731001/1‑150 (MQ=255)
                                                                cATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGagaa                                                                                      <  2:1953941/150‑1 (MQ=255)
                                                                 aTTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGagaag                                                                                     <  2:723210/150‑1 (MQ=255)
                                                                   tCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGAt                                                                                   <  2:3940874/150‑1 (MQ=255)
                                                                   tCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGAt                                                                                   <  2:4077738/150‑1 (MQ=255)
                                                                    cttttcttcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATg                                                                                  >  1:4121535/4‑150 (MQ=255)
                                                                    cTGTTCTTCGCGCTGGTAGTGCCGGGACTTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGACAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATg                                                                                  >  2:3992735/1‑150 (MQ=255)
                                                                     tGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATgg                                                                                 >  1:3461828/1‑150 (MQ=255)
                                                                       ttcttcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGaa                                                                               >  2:771828/1‑150 (MQ=255)
                                                                        tcttcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAg                                                                              >  1:1256272/1‑150 (MQ=255)
                                                                         cttcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGc                                                                             >  2:2671449/1‑150 (MQ=255)
                                                                           tcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCt                                                                           <  1:3296379/150‑1 (MQ=255)
                                                                           tcGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCt                                                                           <  2:3774023/150‑1 (MQ=255)
                                                                            cgcgCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTa                                                                          >  1:3385230/1‑150 (MQ=255)
                                                                                cTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGa                                                                      <  2:1775416/150‑1 (MQ=255)
                                                                                cTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGa                                                                      <  2:2484248/150‑1 (MQ=255)
                                                                                  ggTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGc                                                                    <  2:1195575/150‑1 (MQ=255)
                                                                                       tGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCgg                                                               >  1:3112116/1‑150 (MQ=255)
                                                                                        gCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGgt                                                              >  1:3260991/1‑150 (MQ=255)
                                                                                           gggAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTc                                                           <  1:2404888/150‑1 (MQ=255)
                                                                                                   tgGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGAGTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCa                                                   <  1:1062020/150‑1 (MQ=255)
                                                                                                    ggCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAg                                                  >  1:524085/1‑150 (MQ=255)
                                                                                                      cgcgcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGtt                                                >  2:3443460/1‑150 (MQ=255)
                                                                                                        cgcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGATGATGGAATCCTACGGAGCACCGGTGTCGATCACCAGTTTc                                              <  2:2627122/150‑1 (MQ=255)
                                                                                                         gcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCg                                             <  2:3970752/150‑1 (MQ=255)
                                                                                                         gcCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCg                                             >  1:2626886/1‑150 (MQ=255)
                                                                                                          ccTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCgt                                            <  2:1882264/150‑1 (MQ=255)
                                                                                                            tgtgCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCgtgg                                          >  1:1699263/1‑150 (MQ=255)
                                                                                                             gtgCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCgtggt                                         <  2:1530758/150‑1 (MQ=255)
                                                                                                                cGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGcc                                      <  2:2081827/150‑1 (MQ=255)
                                                                                                                 gggTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCg                                     <  1:2208572/150‑1 (MQ=255)
                                                                                                                 gggTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCg                                     >  2:2139486/1‑150 (MQ=255)
                                                                                                                   gTTAAGCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGAc                                   <  1:1193814/150‑1 (MQ=255)
                                                                                                                      aaGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCgg                                >  1:1182799/1‑150 (MQ=255)
                                                                                                                      aaGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCgg                                >  2:578025/1‑150 (MQ=255)
                                                                                                                      aaGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCgg                                >  2:1046201/1‑150 (MQ=255)
                                                                                                                        gCGTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGtt                              <  1:4130010/150‑1 (MQ=255)
                                                                                                                          gTCTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTAc                            >  1:3409859/1‑150 (MQ=255)
                                                                                                                           tCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTAct                           <  1:720353/150‑1 (MQ=255)
                                                                                                                           tCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTAct                           >  1:194046/1‑150 (MQ=255)
                                                                                                                            cTGGATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTActc                          <  1:3066697/150‑1 (MQ=255)
                                                                                                                            cTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTActc                          <  2:1464112/150‑1 (MQ=255)
                                                                                                                             tGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTActct                         <  2:3476689/150‑1 (MQ=255)
                                                                                                                             tGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGACGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTActct                         >  1:2775/1‑150 (MQ=255)
                                                                                                                              ggATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCtt                        >  2:466781/1‑150 (MQ=255)
                                                                                                                              ggATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCtt                        <  1:377580/150‑1 (MQ=255)
                                                                                                                               gATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCttt                       >  1:1912388/1‑150 (MQ=255)
                                                                                                                               gATCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCttt                       >  1:1444944/1‑150 (MQ=255)
                                                                                                                               gATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCttt                       >  2:650548/1‑150 (MQ=255)
                                                                                                                                aTCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCtttt                      >  1:3709289/1‑150 (MQ=255)
                                                                                                                                 tCCTGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTa                     >  1:2686243/1‑150 (MQ=255)
                                                                                                                                  ccTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTaa                    >  2:2327025/1‑150 (MQ=255)
                                                                                                                                   cTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAAc                   >  1:1507350/1‑150 (MQ=255)
                                                                                                                                    tGATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAAcc                  <  1:3279737/150‑1 (MQ=255)
                                                                                                                                    tGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAAcc                  <  1:1017169/150‑1 (MQ=255)
                                                                                                                                     gATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCt                 >  1:1879805/1‑150 (MQ=255)
                                                                                                                                     gATTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCt                 >  1:3406591/1‑150 (MQ=255)
                                                                                                                                     gATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCt                 <  1:451240/150‑1 (MQ=255)
                                                                                                                                      aTTCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCtt                >  1:2863265/1‑150 (MQ=255)
                                                                                                                                      aTTCGGATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCtt                <  2:2513267/150‑1 (MQ=255)
                                                                                                                                       ttCGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTg               <  2:3037617/150‑1 (MQ=255)
                                                                                                                                       ttCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTg               >  2:3900952/1‑150 (MQ=255)
                                                                                                                                       ttCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTg               <  1:2152593/150‑1 (MQ=255)
                                                                                                                                       ttCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTg               >  2:4087365/1‑150 (MQ=255)
                                                                                                                                         cGTATTCTGAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGAt             <  2:4156046/150‑1 (MQ=255)
                                                                                                                                         cGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGAt             >  2:239455/1‑150 (MQ=255)
                                                                                                                                            aTTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGt          <  1:3960150/150‑1 (MQ=255)
                                                                                                                                             ttCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGtt         <  2:2143098/150‑1 (MQ=255)
                                                                                                                                              tCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTc        <  1:2097984/150‑1 (MQ=255)
                                                                                                                                                tGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGa      <  2:162419/150‑1 (MQ=255)
                                                                                                                                                 gAAATATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGAc     <  1:76250/150‑1 (MQ=255)
                                                                                                                                                  aaaTATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg    >  1:1654037/1‑150 (MQ=255)
                                                                                                                                                  aaaGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACg    >  1:2506457/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGCTAACTTTGGTTTCTCGTCTCTGTGGCCACTGGCGAAACTGGTGCTGCTGGTGCATTTCGCCATTCTGTTCTTCGCGCTGGTAGTGCTGGGAATTGTGGCGCGCCTGTGCGGGTTAAGCGTCTGGATCCTGATTCGTATTCTGAAAGATGAGCTGATTCTGGCGTACTCCACTGCCAGCTCTGAAAGCGTGCTGCCGCGAATTATTGAGAAGATGGAAGCCTACGGAGCACCGGTGTCGATCACCAGTTTCGTGGTGCCGACCGGTTACTCTTTTAACCTTGATGGTTCGACGCT  >  CP009273/4285064‑4285361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑