Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,290,080 G→T 100% I256I (ATC→ATA)  mdtP ← outer membrane factor of efflux pump

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,290,0800GT99.6% 846.1 / ‑4.1 234I256I (ATC→ATA) mdtPouter membrane factor of efflux pump
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (102/131);  total (102/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 234 total aligned reads displayed.

TTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTC  >  CP009273/4289933‑4290226
                                                                                                                                                   |                                                                                                                                                  
ttGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAt                                                                                                                                                  <  1:3620115/150‑1 (MQ=255)
 tGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAtt                                                                                                                                                 <  2:2291707/150‑1 (MQ=255)
 tGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAtt                                                                                                                                                 <  2:538242/150‑1 (MQ=255)
 tGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTAtt                                                                                                                                                 <  1:3261618/150‑1 (MQ=255)
         cGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCttt                                                                                                                                         <  2:250938/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        <  2:1581147/150‑1 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        >  2:1576401/1‑150 (MQ=255)
          gggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTg                                                                                                                                        <  1:4012865/150‑1 (MQ=255)
           ggCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGa                                                                                                                                       <  2:508305/150‑1 (MQ=255)
              gTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACgg                                                                                                                                    <  1:257646/150‑1 (MQ=255)
                   gCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGcaaca                                                                                                                               <  2:3896866/150‑1 (MQ=255)
                    cGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCTTTTGACGGcaacaa                                                                                                                              >  1:1822740/1‑150 (MQ=255)
                     gAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAt                                                                                                                             >  1:2078645/1‑150 (MQ=255)
                      aGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAAtt                                                                                                                            <  2:3317906/150‑1 (MQ=255)
                           cTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGttt                                                                                                                       <  2:1943274/150‑1 (MQ=255)
                              aTAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATc                                                                                                                    <  1:58556/150‑1 (MQ=255)
                               tAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                   >  1:1925645/1‑150 (MQ=255)
                               tAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCg                                                                                                                   <  1:3342040/150‑1 (MQ=255)
                                   agagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCg                                                                                                               >  2:4067526/1‑150 (MQ=255)
                                    gagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGc                                                                                                              >  1:2300601/1‑150 (MQ=255)
                                    gagTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGc                                                                                                              >  1:2686851/1‑150 (MQ=255)
                                        gtCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGa                                                                                                          <  1:2759713/150‑1 (MQ=255)
                                             cGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                                                     <  1:2932338/150‑1 (MQ=255)
                                             cGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtg                                                                                                     <  2:2252813/150‑1 (MQ=255)
                                              ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                    >  1:2497698/1‑150 (MQ=255)
                                              ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                    <  1:339448/150‑1 (MQ=255)
                                              ggAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCtgt                                                                                                    >  1:1262977/1‑150 (MQ=255)
                                                aaTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGc                                                                                                  >  1:3955495/1‑150 (MQ=255)
                                                aaTGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGc                                                                                                  >  2:1288361/1‑150 (MQ=255)
                                                  tGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGccc                                                                                                <  2:3414248/150‑1 (MQ=255)
                                                   gCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcg                                                                                               >  2:757520/1‑150 (MQ=255)
                                                     cGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                                                             <  2:1699851/150‑1 (MQ=255)
                                                     cGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                                                             >  1:707449/1‑150 (MQ=255)
                                                     cGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                                                             <  2:4146595/150‑1 (MQ=255)
                                                     cGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcg                                                                                             >  1:2446803/1‑150 (MQ=255)
                                                       gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgcg                                                                                           >  2:178523/1‑150 (MQ=255)
                                                       gTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCcgcgcg                                                                                           <  2:2401070/150‑1 (MQ=255)
                                                          tGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCg                                                                                        >  1:2349207/1‑150 (MQ=255)
                                                             aCTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTgg                                                                                     >  1:3770471/1‑150 (MQ=255)
                                                             aCTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTgg                                                                                     >  1:485103/1‑150 (MQ=255)
                                                                 gCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAg                                                                                 <  2:854676/150‑1 (MQ=255)
                                                                   ggTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTGTTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGc                                                                               <  1:777759/150‑1 (MQ=255)
                                                                    gTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCa                                                                              <  2:3764339/150‑1 (MQ=255)
                                                                      aaTGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACt                                                                            >  1:305439/1‑150 (MQ=255)
                                                                        tGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                                                          <  1:65356/150‑1 (MQ=255)
                                                                        tGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                                                          >  1:214405/1‑150 (MQ=255)
                                                                        tGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                                                          >  2:660760/1‑150 (MQ=255)
                                                                        tGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTg                                                                          <  1:3544723/150‑1 (MQ=255)
                                                                            aCCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCt                                                                      >  2:1844929/1‑150 (MQ=255)
                                                                             ccGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCtt                                                                     <  1:3023413/150‑1 (MQ=255)
                                                                               ggTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTcc                                                                   <  1:1607781/150‑1 (MQ=255)
                                                                                gTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCa                                                                  >  2:255143/1‑150 (MQ=255)
                                                                                 tCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAg                                                                 <  2:1942384/150‑1 (MQ=255)
                                                                                  cTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGa                                                                >  2:3254418/1‑150 (MQ=255)
                                                                                   tGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAc                                                               >  2:1519194/1‑150 (MQ=255)
                                                                                    gATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGAcc                                                              >  1:844584/1‑150 (MQ=255)
                                                                                       ctcCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTg                                                           <  1:3162790/150‑1 (MQ=255)
                                                                                         ccGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                                                         >  2:738911/1‑150 (MQ=255)
                                                                                         ccGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTgcg                                                         >  2:1217989/1‑150 (MQ=255)
                                                                                            gCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCa                                                      >  1:895333/1‑150 (MQ=255)
                                                                                             cATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCAc                                                     >  1:3875272/1‑150 (MQ=255)
                                                                                               tatCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACtt                                                   <  2:1820215/150‑1 (MQ=255)
                                                                                                atCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACttt                                                  <  1:3317449/150‑1 (MQ=255)
                                                                                                atCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACttt                                                  <  2:414853/150‑1 (MQ=255)
                                                                                                 tCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTa                                                 >  2:1641354/1‑150 (MQ=255)
                                                                                                   gCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTAct                                               <  2:3639036/150‑1 (MQ=255)
                                                                                                    cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                              <  2:3720616/150‑1 (MQ=255)
                                                                                                    cTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTActc                                              >  2:3590119/1‑150 (MQ=255)
                                                                                                        cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                          >  2:2028241/1‑150 (MQ=255)
                                                                                                        cTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGt                                          <  2:4022063/150‑1 (MQ=255)
                                                                                                         tCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTg                                         <  1:60498/150‑1 (MQ=255)
                                                                                                          cccGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgc                                        <  2:3101038/150‑1 (MQ=255)
                                                                                                            cgcgCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTgcgc                                      >  1:230323/1‑150 (MQ=255)
                                                                                                             gcgcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCt                                     >  1:3403644/1‑150 (MQ=255)
                                                                                                               gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt                                   <  2:476775/150‑1 (MQ=255)
                                                                                                               gcCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCttt                                   >  2:1662097/1‑150 (MQ=255)
                                                                                                                ccAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTc                                  <  1:1136760/150‑1 (MQ=255)
                                                                                                                ccAATCAATGCACGCAGAGATTCTCGCGTTTCGGTTATGTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTc                                  <  2:3177927/150‑1 (MQ=255)
                                                                                                                 caatcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCa                                 <  2:3509939/150‑1 (MQ=255)
                                                                                                                    tcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcg                              <  2:931826/150‑1 (MQ=255)
                                                                                                                    tcaatGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACcg                              <  1:1323903/150‑1 (MQ=255)
                                                                                                                        tGCACTCAGATATTCTCGCGTTTAGGTTATTTGCCCTATGACGGCAACACTTTGTTTATCGACCGCCATAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTa                          <  1:1497996/150‑1 (MQ=255)
                                                                                                                        tGCACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTa                          >  1:204754/1‑150 (MQ=255)
                                                                                                                          cACGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaat                        >  1:974754/1‑150 (MQ=255)
                                                                                                                            cGCAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatca                      >  2:3964902/1‑150 (MQ=255)
                                                                                                                              cAGAGATTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTaatcaat                    <  2:3668102/150‑1 (MQ=255)
                                                                                                                                gagaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                  <  2:3078207/150‑1 (MQ=255)
                                                                                                                                gagaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAatcaatca                  <  1:1204678/150‑1 (MQ=255)
                                                                                                                                  gaTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATcaca                >  2:1078732/1‑150 (MQ=255)
                                                                                                                                   aTTCTCGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACAt               >  1:4165345/1‑150 (MQ=255)
                                                                                                                                     tctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCg             >  2:3798934/1‑150 (MQ=255)
                                                                                                                                      ctcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAGGCAATCAGATCGt            >  2:3009458/1‑150 (MQ=255)
                                                                                                                                       tcGCGTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTg           <  2:3866221/150‑1 (MQ=255)
                                                                                                                                          cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt        >  1:344458/1‑150 (MQ=255)
                                                                                                                                          cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt        <  1:1380401/150‑1 (MQ=255)
                                                                                                                                          cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt        >  1:203806/1‑150 (MQ=255)
                                                                                                                                          cgTTTCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGt        <  2:1494898/150‑1 (MQ=255)
                                                                                                                                             ttCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTg     <  2:2446803/150‑1 (MQ=255)
                                                                                                                                              tCGGTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGt    <  1:2124186/150‑1 (MQ=255)
                                                                                                                                                ggTTATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTc  <  1:1003241/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TTGCAGATCCGGGCGTCTGGCGAGCAACTCATAAGAGAGTGTCGCCGGAATGCCGGTCTGGACTTGCGGTAATGCCACCGGTCTGATCTCCGGCATATCGCTGGCTCCCGCGCCAATCAATGCACGCAGAGATTCTCGCGTTTCGGTGATTTGCCCTTTGACGGCAACAATTTGTTTATCGACCGCCAGAATCTGTGCCCGCGCGCCGTGGAAAGGCACTTGCGCTTCCAGACCGTGCGCCACTTTACTCTGGTGCGCTTTCACCGCGTAATCAATCACATCGTGAGTTTGTTC  >  CP009273/4289933‑4290226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑