BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,297,485	G→C	57.4%	G44G (GGC→GGG)	alsK ←	D‑allose kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,297,485	0	G	C	57.4%	198.9 / inf	263	G44G (GGC→GGG)	alsK	D‑allose kinase
Reads supporting (aligned to +/- strand): ref base G (50/62); new base C (91/60); total (141/122)
Fisher's exact test for biased strand distribution p-value = 1.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 267 total aligned reads displayed.

TCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTT  >  CP009273/4297336‑4297633

tCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAgg                                                                                                                                                       <  2:3400763/150‑1 (MQ=255)

  cgccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGcc                                                                                                                                                     >  1:1515944/1‑150 (MQ=255)

   gccgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCg                                                                                                                                                    >  2:672761/1‑150 (MQ=255)

     cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCggg                                                                                                                                                  <  2:164236/150‑1 (MQ=255)

     cgcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCggg                                                                                                                                                  <  2:472975/150‑1 (MQ=255)

      gcTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGa                                                                                                                                                 >  2:2434375/1‑150 (MQ=255)

        tGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                               <  1:1308134/150‑1 (MQ=255)

        tGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGc                                                                                                                                               <  1:144745/150‑1 (MQ=255)

         gTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCa                                                                                                                                              >  1:3273772/1‑150 (MQ=255)

          ttAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCaa                                                                                                                                             <  2:327203/150‑1 (MQ=255)

           tAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAAt                                                                                                                                            >  2:3666270/1‑150 (MQ=255)

               ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                        >  2:3564372/1‑150 (MQ=255)

               ggCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGGTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACt                                                                                                                                        >  2:1096899/1‑150 (MQ=255)

                gCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACtt                                                                                                                                       <  2:713207/150‑1 (MQ=255)

                gCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACtt                                                                                                                                       >  2:1580045/1‑150 (MQ=255)

                   ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTg                                                                                                                                    <  1:1434943/150‑1 (MQ=255)

                   ggTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTg                                                                                                                                    <  1:3939514/150‑1 (MQ=255)

                    gTTAGGCGTAGAAATAATGGTGCGTTTATCATTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGc                                                                                                                                   >  1:3862503/1‑150 (MQ=255)

                      tAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCgg                                                                                                                                 >  1:2386030/1‑150 (MQ=255)

                        ggCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTc                                                                                                                               >  2:1633670/1‑150 (MQ=255)

                         gCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATAACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTcc                                                                                                                              <  2:527261/150‑1 (MQ=255)

                          cGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCg                                                                                                                             <  2:385843/150‑1 (MQ=255)

                          cGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCg                                                                                                                             >  1:1697724/1‑150 (MQ=255)

                          cGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCg                                                                                                                             >  1:2952387/1‑150 (MQ=255)

                            tAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑t                                                                                                                          >  2:2639141/1‑150 (MQ=255)

                            tAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑t                                                                                                                          <  1:2446346/150‑1 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                       >  1:458394/1‑150 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                       >  2:2065485/1‑150 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                       >  2:3827559/1‑150 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                       >  1:660887/1‑150 (MQ=255)

                               aaaTAATGGTGCGTTTATCTTGACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑tttt                                                                                                                       <  1:1257957/150‑1 (MQ=255)

                                aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                      >  1:3968043/1‑150 (MQ=255)

                                aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                      >  1:2240945/1‑150 (MQ=255)

                                aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                      >  2:140330/1‑150 (MQ=255)

                                aataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttt                                                                                                                      >  1:2240874/1‑150 (MQ=255)

                                 ataatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑tatttt                                                                                                                     >  2:2966072/1‑150 (MQ=255)

                                 ataatGGTGAGTTTTTCTTTACTGAACAGCGCAGGAAATCCAATCAACAGACCATGACAGCGAGCTTTAAAGCTCCTGAGTTGCTAGTCAATAATTTCGCCGATTCCCGTCACCAGGCCGGGAGCAATGACTTCTGCGGTCCGA‑tttttt                                                                                                                     <  2:1386681/150‑1 (MQ=255)

                                  taatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑ttttttt                                                                                                                    <  2:400380/150‑1 (MQ=255)

                                   aatGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTc                                                                                                                   >  2:4169130/1‑150 (MQ=255)

                                    atGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCg                                                                                                                  <  2:1518709/150‑1 (MQ=255)

                                     tGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGc                                                                                                                 >  2:2544095/1‑150 (MQ=255)

                                      ggTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCa                                                                                                                >  1:466550/1‑150 (MQ=255)

                                       gTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGAGTTAAAGAGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAg                                                                                                               <  1:2109211/150‑1 (MQ=255)

                                         gCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAgtg                                                                                                             <  1:3070508/150‑1 (MQ=255)

                                           gTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTa                                                                                                           <  1:2158278/150‑1 (MQ=255)

                                                 cTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGttt                                                                                                     <  1:3154372/150‑1 (MQ=255)

                                                     aCTGACCAGCGCCGGAAATCACATCACCAGACAATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTATGAGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCAcc                                                                                                 >  2:2790881/1‑150 (MQ=255)

                                                      cTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCt                                                                                                <  2:262252/150‑1 (MQ=255)

                                                        gACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTc                                                                                              <  2:2904636/150‑1 (MQ=255)

                                                        gACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTc                                                                                              <  1:2043333/150‑1 (MQ=255)

                                                          ccAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctg                                                                                            <  2:225847/150‑1 (MQ=255)

                                                            aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                          <  1:2524346/150‑1 (MQ=255)

                                                            aGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTctgct                                                                                          <  1:1637017/150‑1 (MQ=255)

                                                              cgcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGt                                                                                        <  2:3339085/150‑1 (MQ=255)

                                                               gcCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTc                                                                                       <  2:2691729/150‑1 (MQ=255)

                                                                ccGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTcc                                                                                      <  1:3523988/150‑1 (MQ=255)

                                                                  ggAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGc                                                                                    >  1:1582208/1‑150 (MQ=255)

                                                                     aaTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGa                                                                                 >  2:60346/1‑150 (MQ=255)

                                                                      aTCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGAc                                                                                >  1:197606/1‑150 (MQ=255)

                                                                         ccATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaaa                                                                             >  2:1836380/1‑150 (MQ=255)

                                                                          cATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACaaaa                                                                            <  1:1949634/150‑1 (MQ=255)

                                                                                cAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGat                                                                      >  1:3756478/1‑150 (MQ=255)

                                                                                 aGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGata                                                                     >  2:2209198/1‑150 (MQ=255)

                                                                                  gACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGatat                                                                    <  2:4032654/150‑1 (MQ=255)

                                                                                   aCCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATg                                                                   >  2:2987157/1‑150 (MQ=255)

                                                                                    ccATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTAGTCAATCATTTCGCCGATACCCGACACCAGGCCGTGAGCAATGACTTCTGAGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGATGTCCGCAGACAAAAGCGGATATGc                                                                  <  1:3650632/150‑1 (MQ=255)

                                                                                      aTGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGt                                                                >  1:2924041/1‑150 (MQ=255)

                                                                                       tGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGtt                                                               <  2:2951762/150‑1 (MQ=255)

                                                                                              gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                        >  2:3157268/1‑150 (MQ=255)

                                                                                              gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                        >  2:3036437/1‑150 (MQ=255)

                                                                                              gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCa                                                        <  2:1377461/150‑1 (MQ=255)

                                                                                              gagcgTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGCTTTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGccccc                                                         <  2:4108776/150‑1 (MQ=255)

                                                                                                           ccTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGc                                           >  1:100107/1‑150 (MQ=255)

                                                                                                              gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTac                                        <  2:1341206/150‑1 (MQ=255)

                                                                                                              gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTac                                        <  1:1835113/150‑1 (MQ=255)

                                                                                                              gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTac                                        <  1:2938800/150‑1 (MQ=255)

                                                                                                              gAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTac                                        <  1:2020360/150‑1 (MQ=255)

                                                                                                                gTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacga                                      >  2:1022973/1‑150 (MQ=255)

                                                                                                                 ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCACCATATCCACGCCCGCTacgac                                     <  2:464689/150‑1 (MQ=255)

                                                                                                                 ttGCTCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgac                                     <  1:2660319/150‑1 (MQ=255)

                                                                                                                  tGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTacgacg                                    <  2:3464224/150‑1 (MQ=255)

                                                                                                                    cTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGtt                                  <  2:85505/150‑1 (MQ=255)

                                                                                                                     tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                 >  1:3677466/1‑150 (MQ=255)

                                                                                                                     tCGTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTa                                 <  1:3022215/150‑1 (MQ=255)

                                                                                                                       gTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTTTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCAACGCCCGCTCCGACGTTATg                               >  1:2883117/1‑150 (MQ=255)

                                                                                                                       gTCAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATg                               >  1:2707244/1‑150 (MQ=255)

                                                                                                                         cAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                             >  1:852144/1‑150 (MQ=255)

                                                                                                                         cAATCATTTCGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCt                             >  1:134290/1‑150 (MQ=255)

                                                                                                                            tCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGtt                          >  2:1227369/1‑150 (MQ=255)

                                                                                                                               tttCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGttttt                       <  2:1927644/150‑1 (MQ=255)

                                                                                                                                 tCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGc                     <  1:970192/150‑1 (MQ=255)

                                                                                                                                  cGCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCa                    >  2:1705765/1‑150 (MQ=255)

                                                                                                                                   gCCGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCAt                   <  1:4116274/150‑1 (MQ=255)

                                                                                                                                    ccGATACCCGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATg                  <  1:1197518/150‑1 (MQ=255)

                                                                                                                                       aTACCCGACCCCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATCTCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAgg               <  2:1120940/150‑1 (MQ=255)

                                                                                                                                           ccGACACCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGt           >  2:2001667/1‑150 (MQ=255)

                                                                                                                                              acacCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTAcc        >  2:1990641/1‑150 (MQ=255)

                                                                                                                                               cacCAGCCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCt       <  2:3711323/150‑1 (MQ=255)

                                                                                                                                                   aGGCCGGGAGCTTTGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCAGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCt   <  2:2757644/150‑1 (MQ=255)

                                                                                                                                                    ggCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTGTTTGCATGAGGCTGTACCTCGCtt  >  1:2400979/1‑150 (MQ=255)

TCCGCCGCTGTTAACGGCAGGTTAGGCGTAGAAATAATGGTGCGTTTATCTTTACTGACCAGCGCCGGAAATCCCATCACCAGACCATGACAGCGAGCGTTAAAGCGCCTGAGTTGCTCGTCAATCATTTCGCCGATACCCGACACCAGGCCGGGAGCAATGACTTCTGCGGTCCGC‑TTTTTTTCGCAGTGTAGCGTTTCACCTTCTGCTGTCCGCAGACAAAAGCGGATATGCGTTGCCCCCATATCCACGCCCGCTACGACGTTATGCTGTTTTTGCATGAGGCTGTACCTCGCTT  >  CP009273/4297336‑4297633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`