BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,394,391	G→T	6.3%	T63T (ACG→ACT)	yjeT →	DUF2065 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,394,391	0	G	T	6.3%	1101.4 / 32.7	285	T63T (ACG→ACT)	yjeT	DUF2065 family protein
Reads supporting (aligned to +/- strand): ref base G (124/143); new base T (13/5); total (137/148)
Fisher's exact test for biased strand distribution p-value = 4.92e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 290 total aligned reads displayed.

GTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394242‑4394540

gTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACg                                                                                                                                                       >  1:2807879/1‑150 (MQ=255)

  aCTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGAt                                                                                                                                                     <  1:1235999/150‑1 (MQ=255)

          gTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGaa                                                                                                                                             <  2:1613683/150‑1 (MQ=255)

          gTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGaa                                                                                                                                             >  2:3184557/1‑150 (MQ=255)

           tttAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAAc                                                                                                                                            <  1:3204754/150‑1 (MQ=255)

           tttAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAAc                                                                                                                                            <  1:613552/150‑1 (MQ=255)

           tttAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAAc                                                                                                                                            >  2:3310391/1‑150 (MQ=255)

            ttAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAAGATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACa                                                                                                                                           <  1:2218641/150‑1 (MQ=255)

              aGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaa                                                                                                                                         >  1:2287647/1‑150 (MQ=255)

                 gCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACaaaaaa                                                                                                                                      <  1:2738748/150‑1 (MQ=255)

                     aTGCTTTACCCTAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGa                                                                                                                                  <  1:1912123/150‑1 (MQ=255)

                       gCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACt                                                                                                                                <  1:1308696/150‑1 (MQ=255)

                       gCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACt                                                                                                                                >  2:522517/1‑150 (MQ=255)

                        cTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATCTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGAGGGCGTTGTGGTCTACTACATGTGGAGGAAAACGATAGGCTGAACAAAAAACAGACTg                                                                                                                               >  2:1928615/1‑150 (MQ=255)

                         tttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGa                                                                                                                              <  2:2726738/150‑1 (MQ=255)

                          ttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGAt                                                                                                                             >  2:121511/1‑150 (MQ=255)

                          ttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGAt                                                                                                                             <  1:2487805/150‑1 (MQ=255)

                          ttACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGAt                                                                                                                             <  1:1385272/150‑1 (MQ=255)

                           tACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATc                                                                                                                            <  2:2214220/150‑1 (MQ=255)

                            aCCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCg                                                                                                                           >  2:3864605/1‑150 (MQ=255)

                             cccGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGa                                                                                                                          >  2:39817/1‑150 (MQ=255)

                              ccGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAg                                                                                                                         <  1:1465751/150‑1 (MQ=255)

                                gAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGt                                                                                                                       <  1:3221665/150‑1 (MQ=255)

                                     cATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACCGATCGAGGTCAttt                                                                                                                  >  1:474927/1‑150 (MQ=255)

                                      aTGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCAtttt                                                                                                                 >  1:2344612/1‑150 (MQ=255)

                                        ggAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTg                                                                                                               >  2:146959/1‑150 (MQ=255)

                                         gaagaaGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACAACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGa                                                                                                              >  1:1762666/1‑150 (MQ=255)

                                             aagaTGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGc                                                                                                          <  2:2108469/150‑1 (MQ=255)

                                                atgatCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCaaa                                                                                                       <  2:44574/150‑1 (MQ=255)

                                                 tgatCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCaaaa                                                                                                      <  1:3164154/150‑1 (MQ=255)

                                                    tctcTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGt                                                                                                   >  1:652903/1‑150 (MQ=255)

                                                    tctcTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGt                                                                                                   >  1:199412/1‑150 (MQ=255)

                                                    tctcTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGt                                                                                                   <  1:86737/150‑1 (MQ=255)

                                                     ctctGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTg                                                                                                  >  2:3064235/1‑150 (MQ=255)

                                                        tGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTg                                                                                               <  1:2630226/150‑1 (MQ=255)

                                                           gATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTaa                                                                                            >  1:3748209/1‑150 (MQ=255)

                                                               aCCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActct                                                                                        <  2:3142406/150‑1 (MQ=255)

                                                               aCCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAActct                                                                                        <  1:1194259/150‑1 (MQ=255)

                                                                 cAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGa                                                                                      >  2:3785416/1‑150 (MQ=255)

                                                                 cAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGa                                                                                      >  1:835768/1‑150 (MQ=255)

                                                                  aaTTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGaa                                                                                     <  1:2650009/150‑1 (MQ=255)

                                                                      tGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCTAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAg                                                                                 >  2:265523/1‑150 (MQ=255)

                                                                        cccGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCg                                                                               >  2:3647251/1‑150 (MQ=255)

                                                                          cGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTNCAAAAAGTGCTGTAACTCTGAAAAAGCGAt                                                                             <  1:3854170/150‑1 (MQ=255)

                                                                          cGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGAt                                                                             <  1:2460808/150‑1 (MQ=255)

                                                                             taataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGt                                                                          >  2:2856143/1‑150 (MQ=255)

                                                                             taataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGt                                                                          >  2:3086804/1‑150 (MQ=255)

                                                                              aataTTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTa                                                                         >  2:2428923/1‑150 (MQ=255)

                                                                                tatTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGa                                                                       >  1:3916696/1‑150 (MQ=255)

                                                                                  ttttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGTTCGAGGTCATTTTTGAGTGCAAATAGTGCTGTAACTCTGAAAAAGCGATGGTAGAAt                                                                     <  2:3533427/150‑1 (MQ=255)

                                                                                  ttttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAAt                                                                     <  2:1095475/150‑1 (MQ=255)

                                                                                  ttttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAAt                                                                     <  1:2460458/150‑1 (MQ=255)

                                                                                  ttttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAAt                                                                     <  2:176578/150‑1 (MQ=255)

                                                                                   tttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTTGTATACTACATGTTTAGGAAAACGATTGGCTGATCAAAATAGAGTCTGATCGTGGTCATTTTTGAGTGCAAATAGTGCTGTAACTCTTAAAAAGCGATGGTAGAATc                                                                    <  1:3438720/150‑1 (MQ=255)

                                                                                   tttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGTCTGAACAAAAAACAGACTGATCGAGGTCATTTTTTAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATc                                                                    >  1:1843103/1‑150 (MQ=255)

                                                                                   tttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATc                                                                    >  1:1152182/1‑150 (MQ=255)

                                                                                    ttACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATcc                                                                   <  1:3704717/150‑1 (MQ=255)

                                                                                      aCGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAt                                                                 <  2:413059/150‑1 (MQ=255)

                                                                                        gtcgtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCAttt                                                               >  1:3583619/1‑150 (MQ=255)

                                                                                           gtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTa                                                            >  2:2813371/1‑150 (MQ=255)

                                                                                           gtTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTa                                                            <  2:2513705/150‑1 (MQ=255)

                                                                                            ttttGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTaa                                                           >  2:4020528/1‑150 (MQ=255)

                                                                                               tGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCa                                                        <  1:1574966/150‑1 (MQ=255)

                                                                                                  cGGGGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATGGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAAc                                                     <  2:2027759/150‑1 (MQ=255)

                                                                                                   ggTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTACAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACg                                                    >  2:1298703/1‑150 (MQ=255)

                                                                                                    gTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACgg                                                   <  2:2982720/150‑1 (MQ=255)

                                                                                                      ggACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTg                                                 <  2:1577439/150‑1 (MQ=255)

                                                                                                      ggACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTg                                                 <  2:2287647/150‑1 (MQ=255)

                                                                                                        aCTTTTGGTTGCGGGCGTTTTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGAt                                               <  1:3993840/150‑1 (MQ=255)

                                                                                                            gtgGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTg                                           <  1:1647393/150‑1 (MQ=255)

                                                                                                             tgGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGa                                          <  2:2244318/150‑1 (MQ=255)

                                                                                                              ggTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGaa                                         >  1:2846734/1‑150 (MQ=255)

                                                                                                                 tggggggGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTAATTTTTGTGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGtaaaa                                      <  1:1411096/147‑1 (MQ=255)

                                                                                                                 tGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGaaaaa                                      >  1:811826/1‑150 (MQ=255)

                                                                                                                   cGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATg                                    >  1:777599/1‑150 (MQ=255)

                                                                                                                      gCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGt                                 <  1:3066547/150‑1 (MQ=255)

                                                                                                                       cGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTa                                <  2:479761/150‑1 (MQ=255)

                                                                                                                        gTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaa                               >  1:2679592/1‑150 (MQ=255)

                                                                                                                        gTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaa                               >  2:707428/1‑150 (MQ=255)

                                                                                                                        gTTGTGGTCTACTACATGTTGAGGAAAACTATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaa                               >  2:584577/1‑150 (MQ=255)

                                                                                                                         ttGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaac                              >  1:3286188/1‑150 (MQ=255)

                                                                                                                          tgtgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaaca                             <  1:1278380/150‑1 (MQ=255)

                                                                                                                          tgtgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaaca                             >  1:105933/1‑150 (MQ=255)

                                                                                                                           gtgGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTaacaa                            >  2:3421803/1‑150 (MQ=255)

                                                                                                                             ggTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcg                          >  2:938059/1‑150 (MQ=255)

                                                                                                                             ggTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcg                          >  2:1011760/1‑150 (MQ=255)

                                                                                                                                  actacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtc                     <  2:3353016/150‑1 (MQ=255)

                                                                                                                                  actacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtc                     <  1:718679/150‑1 (MQ=255)

                                                                                                                                   ctacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtcg                    >  2:1989701/1‑150 (MQ=255)

                                                                                                                                   ctacATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAAcgtcgtcg                    >  2:787235/1‑150 (MQ=255)

                                                                                                                                       aTGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACt                <  2:1714312/150‑1 (MQ=255)

                                                                                                                                        tGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTg               <  1:3339144/150‑1 (MQ=255)

                                                                                                                                         gTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTgg              <  2:1852436/150‑1 (MQ=255)

                                                                                                                                          ttGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTggg             >  1:3826526/1‑150 (MQ=255)

                                                                                                                                            gAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGATGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAAAAACGTCGTCGTACTGGGCa           <  2:1182093/150‑1 (MQ=255)

                                                                                                                                               gAAAACGAATGGCTGAACAAAAAACAGACTGATACAGGACATATATGAGTTCAAAAAGAGCAGTAACTCACAAAAAGAGAAAGAACAATCCATAATTAAGCAAAAAGTGATAAAGAAAAATGCGTACCAACGTCGACGTACTTGACAccc        >  2:3480684/1‑150 (MQ=255)

                                                                                                                                                  aaCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTATTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAAt     <  2:3738286/150‑1 (MQ=255)

                                                                                                                                                   aCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATg    <  1:3773738/150‑1 (MQ=255)

                                                                                                                                                   aCGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATg    <  1:1421459/150‑1 (MQ=255)

                                                                                                                                                    cGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATgg   >  1:1083973/1‑150 (MQ=255)

GTACTGGAAGGTTTAGGGCCGATGCTTTACCCGAAGGCATGGAAGAAGATGATCTCTGCGATGACCAATTTGCCCGATAATATTTTACGTCGTTTTGGCGGTGGACTTGTGGTTGCGGGCGTTGTGGTCTACTACATGTTGAGGAAAACGATTGGCTGAACAAAAAACAGACTGATCGAGGTCATTTTTGAGTGCAAAAAGTGCTGTAACTCTGAAAAAGCGATGGTAGAATCCATTTTTAAGCAAACGGTGATTTTGAAAAATGGGTAACAACGTCGTCGTACTGGGCACCCAATGGG  >  CP009273/4394242‑4394540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`