Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,402,850 G→T 53.8% L2L (CTG→CTT)  yjfC → ATP‑Grasp family ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,402,8500GT53.8% 16.5 / inf 236L2L (CTG→CTT) yjfCATP‑Grasp family ATPase
Reads supporting (aligned to +/- strand):  ref base G (56/53);  new base T (68/59);  total (124/112)
Fisher's exact test for biased strand distribution p-value = 7.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 240 total aligned reads displayed.

TTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAA  >  CP009273/4402701‑4402997
                                                                                                                                                      |                                                                                                                                                   
ttCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTg                                                                                                                                                     <  1:3309743/150‑1 (MQ=255)
  cTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGGGGGCATTGGGGAGGCTAATCATGCTgag                                                                                                                                                   >  1:1138175/1‑150 (MQ=255)
       gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGAcaca                                                                                                                                              <  1:2837631/150‑1 (MQ=255)
       gATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGAcaca                                                                                                                                              <  1:2837614/150‑1 (MQ=255)
              ccTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTcc                                                                                                                                       <  1:4168314/150‑1 (MQ=255)
              ccTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTcc                                                                                                                                       <  2:351645/150‑1 (MQ=255)
               cTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCt                                                                                                                                      <  2:1760836/150‑1 (MQ=255)
                tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCtg                                                                                                                                     <  1:2981043/150‑1 (MQ=255)
                tGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtg                                                                                                                                     >  2:261846/1‑150 (MQ=255)
                 ggCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtgt                                                                                                                                    >  2:1759639/1‑150 (MQ=255)
                  gcgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCtgtg                                                                                                                                   <  2:1822386/150‑1 (MQ=255)
                   cgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGc                                                                                                                                  >  1:1012520/1‑150 (MQ=255)
                   cgcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGc                                                                                                                                  <  2:1209645/150‑1 (MQ=255)
                    gcTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCg                                                                                                                                 <  2:3082256/150‑1 (MQ=255)
                      tCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGAc                                                                                                                               <  2:3886708/150‑1 (MQ=255)
                          gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAATAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                           <  1:228696/150‑1 (MQ=255)
                          gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                           >  1:981585/1‑150 (MQ=255)
                          gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                           <  1:2352233/150‑1 (MQ=255)
                          gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGa                                                                                                                           <  2:1051431/150‑1 (MQ=255)
                          gTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGa                                                                                                                           <  1:2612276/150‑1 (MQ=255)
                            tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATc                                                                                                                         <  2:141028/150‑1 (MQ=255)
                            tCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCAAGCTGAGACACAACGTTCCTGTGCGACGGGATc                                                                                                                         >  1:3743171/1‑150 (MQ=255)
                             cTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCt                                                                                                                        <  2:3618639/150‑1 (MQ=255)
                                  aaaaaaGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGAcc                                                                                                                   <  2:3985285/150‑1 (MQ=255)
                                  aaaaaaGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGAcc                                                                                                                   >  2:1124273/1‑150 (MQ=255)
                                  aaaaaaGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGAcc                                                                                                                   >  2:78494/1‑150 (MQ=255)
                                    aaaaGAGTCTTACTCTTCGGGCGGCTTTACAACC‑AAAAAAGCGTCTACCGTATCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTACCTGTGCGACGGGATCTGGCCCAg                                                                                                                 >  2:1074158/1‑150 (MQ=255)
                                     aaaGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGTGAGTCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGa                                                                                                                >  2:441945/1‑150 (MQ=255)
                                      aaGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGAt                                                                                                               <  2:3466638/150‑1 (MQ=255)
                                       agagTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                              <  1:871230/150‑1 (MQ=255)
                                       agagTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATc                                                                                                              <  2:1987278/150‑1 (MQ=255)
                                              tACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTg                                                                                                       >  2:1615918/1‑150 (MQ=255)
                                               aCTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGa                                                                                                      >  1:963562/1‑150 (MQ=255)
                                               aCTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGa                                                                                                      <  2:2523689/150‑1 (MQ=255)
                                                   ttCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAAc                                                                                                  <  2:2101174/150‑1 (MQ=255)
                                                    tCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACg                                                                                                 <  2:3132381/150‑1 (MQ=255)
                                                    tCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACg                                                                                                 >  1:2999595/1‑150 (MQ=255)
                                                      gggCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGAGCTGGACCAGATCGCCGCTGACAACGGt                                                                                               >  2:798082/1‑150 (MQ=255)
                                                        gcggcTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGttt                                                                                             <  1:1559994/150‑1 (MQ=255)
                                                        gcggcTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGttt                                                                                             <  2:2386493/150‑1 (MQ=255)
                                                          ggcTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCg                                                                                           <  1:1810868/150‑1 (MQ=255)
                                                             tttACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                        <  1:1129208/150‑1 (MQ=255)
                                                             tttACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACt                                                                                        <  1:1842628/150‑1 (MQ=255)
                                                              ttACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACtt                                                                                       >  2:584740/1‑150 (MQ=255)
                                                               tACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACttt                                                                                      <  2:539505/150‑1 (MQ=255)
                                                                accaccaAAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACttt                                                                                      <  1:2245916/150‑1 (MQ=255)
                                                                accacc‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCTGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTc                                                                                     <  1:700576/150‑1 (MQ=255)
                                                                    cc‑aAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCatat                                                                                 <  2:332056/150‑1 (MQ=255)
                                                                     c‑aAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatc                                                                                >  1:481430/1‑150 (MQ=255)
                                                                         aaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcatc                                                                             >  1:396017/1‑150 (MQ=255)
                                                                         aaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcatc                                                                             >  1:142481/1‑150 (MQ=255)
                                                                         aaaaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCAGTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATatcatc                                                                             >  1:142389/1‑150 (MQ=255)
                                                                           aaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGa                                                                           <  2:903992/150‑1 (MQ=255)
                                                                           aaGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGa                                                                           <  2:35463/150‑1 (MQ=255)
                                                                            aGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGAc                                                                          <  2:3310093/150‑1 (MQ=255)
                                                                              cGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACaa                                                                        <  1:2666825/150‑1 (MQ=255)
                                                                               gTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAAt                                                                       <  1:4112429/150‑1 (MQ=255)
                                                                                     cGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAATGGTTTCGACTTTCATATCATCGACAATGAAATc                                                                 >  1:21617/1‑150 (MQ=255)
                                                                                     cGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATc                                                                 <  1:677185/150‑1 (MQ=255)
                                                                                     agTTTCTCGCGGCGGCGATGGTCGTTCTACCAGCGCCCGTGGGCATTGGGGATGCAAAACATGCAGAGACACACCGATCCTGTGCGACGGGATCTGGACCAGATCGCCGCAGACAACGGTTACGACTATCATATCAACGACAATGAAATc                                                                 >  2:1333615/2‑150 (MQ=255)
                                                                                      gTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCt                                                                >  2:2315891/1‑150 (MQ=255)
                                                                                       tttCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa                                                               >  1:3253852/1‑150 (MQ=255)
                                                                                       tttCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTa                                                               >  2:3779826/1‑150 (MQ=255)
                                                                                        ttCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTAt                                                              >  2:2779847/1‑150 (MQ=255)
                                                                                                gcggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATGGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATga                                                      <  2:1280800/150‑1 (MQ=255)
                                                                                                  ggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATgaga                                                    >  2:3980677/1‑150 (MQ=255)
                                                                                                  ggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATgaga                                                    <  1:3091201/150‑1 (MQ=255)
                                                                                                  ggcTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATgaga                                                    <  1:2743236/150‑1 (MQ=255)
                                                                                                     tATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTc                                                 >  1:206173/1‑150 (MQ=255)
                                                                                                      aTGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCg                                                <  2:637969/150‑1 (MQ=255)
                                                                                                      aTGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCg                                                <  1:122590/150‑1 (MQ=255)
                                                                                                       tGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCgg                                               >  2:2727019/1‑150 (MQ=255)
                                                                                                        ggTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCggg                                              >  1:3777209/1‑150 (MQ=255)
                                                                                                           cGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCtt                                           >  2:819109/1‑150 (MQ=255)
                                                                                                           cGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCtt                                           <  1:332580/150‑1 (MQ=255)
                                                                                                             ttcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAc                                         >  1:1237111/1‑150 (MQ=255)
                                                                                                             ttcttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTAc                                         <  1:1278688/150‑1 (MQ=255)
                                                                                                                ttcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGt                                      >  1:2904378/1‑150 (MQ=255)
                                                                                                                 tcCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtt                                     <  1:2389896/150‑1 (MQ=255)
                                                                                                                   cAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCAACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGtttt                                   >  1:196491/1‑150 (MQ=255)
                                                                                                                    aGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAACCTATTGGGATGAGAGTCGGGCTTACCGTTTTa                                  >  2:3768767/1‑150 (MQ=255)
                                                                                                                      cgcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTAct                                >  1:2518388/1‑150 (MQ=255)
                                                                                                                       gcCCGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActc                               >  2:1834247/1‑150 (MQ=255)
                                                                                                                        cccGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTActct                              >  2:1687858/1‑150 (MQ=255)
                                                                                                                          cGTGGGCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTgc                            >  1:2589775/1‑150 (MQ=255)
                                                                                                                              ggCATTGGGGAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCa                        >  2:3455915/1‑150 (MQ=255)
                                                                                                                                cATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGa                      >  1:3570243/1‑150 (MQ=255)
                                                                                                                                 aTTGGGGAGGCGAATCCTGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCGGACAACGGTTTGGACTTTCATATCAGCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAt                     <  2:923350/150‑1 (MQ=255)
                                                                                                                                  ttGGGGAGGCTAATCATGCTGAGTCACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAtt                    <  2:848981/150‑1 (MQ=255)
                                                                                                                                  ttGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGAtt                    <  2:874216/150‑1 (MQ=255)
                                                                                                                                    ggggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGa                  <  2:1225809/150‑1 (MQ=255)
                                                                                                                                    ggggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGa                  <  1:3490990/150‑1 (MQ=255)
                                                                                                                                     gggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGaa                 <  2:670937/150‑1 (MQ=255)
                                                                                                                                     gggAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGaa                 <  2:268815/150‑1 (MQ=255)
                                                                                                                                      ggAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAag                <  1:1694179/150‑1 (MQ=255)
                                                                                                                                       gAGGCTAATCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAaga               <  1:258068/150‑1 (MQ=255)
                                                                                                                                             aaTCATGCTTAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGAt         >  2:1938320/1‑150 (MQ=255)
                                                                                                                                             aaTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGAt         <  2:3507742/150‑1 (MQ=255)
                                                                                                                                              aTCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATc        >  1:3050989/1‑150 (MQ=255)
                                                                                                                                                  tGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGaaa    <  2:3414724/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
TTCTGGTGATTACTCCTGGCGCTCCGGTTCTGGCAAAAAAGAGTCTTACTCTTCGGGCGGCTTTACCACC‑AAAAAAGCGTCTACCGTTTCTCGCGGCGGCTATGGTCGTTCTTCCAGCGCCCGTGGGCATTGGGGAGGCTAATCATGCTGAGACACAACGTTCCTGTGCGACGGGATCTGGACCAGATCGCCGCTGACAACGGTTTCGACTTTCATATCATCGACAATGAAATCTATTGGGATGAGAGTCGGGCTTACCGTTTTACTCTGCGCCAGATTGAAGAGCAGATCGAAAAA  >  CP009273/4402701‑4402997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑