Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 444,489 G→A 100% R537C (CGT→TGT)  cyoB ← cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273444,4890GA99.5% 793.6 / ‑4.4 219R537C (CGT→TGT) cyoBcytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (127/91);  total (127/92)
Fisher's exact test for biased strand distribution p-value = 4.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

CATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGT  >  CP009273/444343‑444635
                                                                                                                                                  |                                                                                                                                                  
cATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCACTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGTACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGcc                                                                                                                                                 >  2:1417269/1‑150 (MQ=255)
     gAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCAcccc                                                                                                                                            >  1:2706860/1‑150 (MQ=255)
      aaTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCa                                                                                                                                           >  2:2081302/1‑150 (MQ=255)
       aTTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCAc                                                                                                                                          <  1:216149/150‑1 (MQ=255)
         ttcttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACgg                                                                                                                                        >  1:1575579/1‑150 (MQ=255)
           cttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGt                                                                                                                                      >  2:1756639/1‑150 (MQ=255)
           cttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGt                                                                                                                                      >  1:1827760/1‑150 (MQ=255)
            ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTc                                                                                                                                     >  1:508186/1‑150 (MQ=255)
            ttcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTc                                                                                                                                     >  2:1072705/1‑150 (MQ=255)
             tcATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCg                                                                                                                                    <  1:1094811/150‑1 (MQ=255)
                tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCa                                                                                                                                 >  1:3229780/1‑150 (MQ=255)
                tAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCa                                                                                                                                 <  2:376471/150‑1 (MQ=255)
                 aGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAg                                                                                                                                >  2:2313187/1‑150 (MQ=255)
                  gTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGt                                                                                                                               <  1:94654/150‑1 (MQ=255)
                          gCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCAc                                                                                                                       >  1:2402098/1‑150 (MQ=255)
                               ttATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTc                                                                                                                  >  2:4043402/1‑150 (MQ=255)
                                tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCt                                                                                                                 <  2:3018167/150‑1 (MQ=255)
                                tataCGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCt                                                                                                                 <  2:1791121/150‑1 (MQ=255)
                                    cGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtc                                                                                                             >  2:3102954/1‑150 (MQ=255)
                                      cTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgc                                                                                                           >  1:3246750/1‑150 (MQ=255)
                                         cGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggt                                                                                                        >  1:2802948/1‑150 (MQ=255)
                                         cGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggt                                                                                                        >  2:1689648/1‑150 (MQ=255)
                                          gCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                       <  1:2100810/150‑1 (MQ=255)
                                          gCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                       >  2:2097144/1‑150 (MQ=255)
                                          gCCTTTCTCTTTCATGTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtc                                                                                                       <  1:2159669/150‑1 (MQ=255)
                                            cTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTggtcgcggtcgc                                                                                                     >  2:1617831/1‑150 (MQ=255)
                                              ttCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGa                                                                                                   >  2:1973078/1‑150 (MQ=255)
                                               tctcTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGaa                                                                                                  >  1:2678690/1‑150 (MQ=255)
                                                ctctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAAt                                                                                                 <  1:2259470/150‑1 (MQ=255)
                                                 tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATa                                                                                                >  2:1737711/1‑150 (MQ=255)
                                                 tctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATa                                                                                                <  2:793202/150‑1 (MQ=255)
                                                  ctTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAg                                                                                               >  1:4000118/1‑150 (MQ=255)
                                                      cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCCCGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                           <  2:1866871/150‑1 (MQ=255)
                                                      cATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAAc                                                                                           <  2:1794069/150‑1 (MQ=255)
                                                       aTTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACg                                                                                          >  2:2379405/1‑150 (MQ=255)
                                                         ttCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTa                                                                                        <  2:3709521/150‑1 (MQ=255)
                                                          tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                       <  2:1718838/150‑1 (MQ=255)
                                                          tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                       <  2:1637912/150‑1 (MQ=255)
                                                          tCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTAc                                                                                       <  1:30905/150‑1 (MQ=255)
                                                            ccAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACAt                                                                                     <  2:1566455/150‑1 (MQ=255)
                                                             cAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                                                    >  2:2758746/1‑150 (MQ=255)
                                                              aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  2:1455207/1‑150 (MQ=255)
                                                              aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  1:2745913/1‑150 (MQ=255)
                                                              aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   <  1:3142921/150‑1 (MQ=255)
                                                              aGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCt                                                                                   >  1:720810/1‑150 (MQ=255)
                                                                aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                                                                                 <  1:2911710/150‑1 (MQ=255)
                                                                aaTGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGa                                                                                 >  2:1282648/1‑150 (MQ=255)
                                                                     aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc                                                                            >  2:3607707/1‑150 (MQ=255)
                                                                     aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc                                                                            <  2:566885/150‑1 (MQ=255)
                                                                     aTCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAAc                                                                            >  2:1190918/1‑150 (MQ=255)
                                                                      tCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACg                                                                           <  1:3926492/150‑1 (MQ=255)
                                                                         cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg                                                                        <  2:1956972/150‑1 (MQ=255)
                                                                         cGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAgg                                                                        >  2:3005718/1‑150 (MQ=255)
                                                                          gTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                                                       >  1:1817502/1‑150 (MQ=255)
                                                                             cGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCaga                                                                    >  2:3673490/1‑150 (MQ=255)
                                                                                 aaCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                                <  1:1109546/150‑1 (MQ=255)
                                                                                 aaCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                                >  1:3743925/1‑150 (MQ=255)
                                                                                 aaCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAAt                                                                <  1:2629678/150‑1 (MQ=255)
                                                                                 aaCGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGCTTCTGGTCGCGGTCGCGAATATAAACGTACATCTGAATAACGAGGCAGAGAAt                                                                >  2:3130279/1‑150 (MQ=255)
                                                                                    gTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATAcc                                                             >  1:2836146/1‑150 (MQ=255)
                                                                                      gCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCa                                                           <  2:3938244/150‑1 (MQ=255)
                                                                                        ggCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgc                                                         >  2:373783/1‑150 (MQ=255)
                                                                                           aCTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCa                                                      <  2:1302226/150‑1 (MQ=255)
                                                                                               cGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCa                                                  <  2:3688432/150‑1 (MQ=255)
                                                                                               cGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCa                                                  <  2:1135517/150‑1 (MQ=255)
                                                                                               cGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCa                                                  >  2:2213796/1‑150 (MQ=255)
                                                                                                 gCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGt                                                >  1:3487634/1‑150 (MQ=255)
                                                                                                  cAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTa                                               >  1:1620683/1‑150 (MQ=255)
                                                                                                  cAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTa                                               >  2:1355773/1‑150 (MQ=255)
                                                                                                    aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACt                                             <  2:3257439/150‑1 (MQ=255)
                                                                                                    aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACt                                             >  2:3769775/1‑150 (MQ=255)
                                                                                                       ttATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCa                                          >  2:1023635/1‑150 (MQ=255)
                                                                                                        tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                         <  1:1007020/150‑1 (MQ=255)
                                                                                                        tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                         <  1:3715767/150‑1 (MQ=255)
                                                                                                        tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                         >  1:1328161/1‑150 (MQ=255)
                                                                                                        tataGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCAc                                         >  1:270413/1‑150 (MQ=255)
                                                                                                           aGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgc                                      >  2:3424786/1‑150 (MQ=255)
                                                                                                              aCGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctgg                                   <  2:617179/150‑1 (MQ=255)
                                                                                                                ggAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggct                                 >  2:278876/1‑150 (MQ=255)
                                                                                                                ggAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggct                                 >  1:752708/1‑150 (MQ=255)
                                                                                                                 gAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggctg                                >  1:3015812/1‑150 (MQ=255)
                                                                                                                     cGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAat                            <  2:464138/150‑1 (MQ=255)
                                                                                                                     cGGGGAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAat                            <  1:663172/150‑1 (MQ=255)
                                                                                                                      ggggAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatc                           >  1:2017151/1‑150 (MQ=255)
                                                                                                                         gAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcatc                        <  2:2553288/150‑1 (MQ=255)
                                                                                                                         gAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcatc                        >  2:1103715/1‑150 (MQ=255)
                                                                                                                         gAAGAGGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAatcatc                        <  2:294642/150‑1 (MQ=255)
                                                                                                                            gagGTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGc                     <  1:2146996/150‑1 (MQ=255)
                                                                                                                              ggTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                   >  2:272606/1‑150 (MQ=255)
                                                                                                                              ggTTGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCAt                   <  2:3511829/150‑1 (MQ=255)
                                                                                                                                 tGCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt                <  1:815461/150‑1 (MQ=255)
                                                                                                                                 agCCCACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGgt                <  1:1847712/149‑1 (MQ=255)
                                                                                                                                    ccACTCCAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGtgtg             >  2:2117627/1‑150 (MQ=255)
                                                                                                                                        tCCATCTTACAGCCACCACAAGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGAGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACACAGCGCAATAAGAACTTCACCGCTGGCTTCAATCATCAGCATGTTGAGTAAc         >  2:234356/1‑150 (MQ=255)
                                                                                                                                         ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACt        <  1:2936087/150‑1 (MQ=255)
                                                                                                                                         ccAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACt        <  2:660124/150‑1 (MQ=255)
                                                                                                                                          cAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg       <  1:1917460/150‑1 (MQ=255)
                                                                                                                                          cAGCGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTg       >  1:3947105/1‑150 (MQ=255)
                                                                                                                                             cGTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAAACAACAGCATGGTGTGGAACTGCgg    >  1:4163061/1‑150 (MQ=255)
                                                                                                                                              gTACAGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCggg   >  2:558095/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CATATGAATTTCTTCATAGTGGTCAGGCTTTTTATACGCTTCGCCTTTCTCTTTCATTTCCCAGAATGCATCACGTTCGTGAACGTGCGGCACTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGCTGCAATCATCAGCATGGTGTGGAACTGCGGGT  >  CP009273/444343‑444635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑