BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,465,624	C→T	35.3%	S124F (TCC→TTC)	yjgL →	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,465,624	0	C	T	35.3%	375.8 / inf	297	S124F (TCC→TTC)	yjgL	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base C (108/84); new base T (58/47); total (166/131)
Fisher's exact test for biased strand distribution p-value = 9.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 304 total aligned reads displayed.

CAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTT  >  CP009273/4465475‑4465773

cAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTc                                                                                                                                                       >  1:4129537/1‑150 (MQ=255)

cAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTc                                                                                                                                                       >  1:2028906/1‑150 (MQ=255)

 aaCGAAGCGGGTAGAAATATGATGTCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGATTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTcc                                                                                                                                                      <  1:821159/150‑1 (MQ=255)

  aCGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCa                                                                                                                                                     <  1:178492/150‑1 (MQ=255)

   cGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACACAAGGTGATTATAGAAATTTCACTTTCCAc                                                                                                                                                    <  1:2534105/150‑1 (MQ=255)

    gAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACt                                                                                                                                                   >  2:56774/1‑150 (MQ=255)

    gAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACt                                                                                                                                                   <  1:3049794/150‑1 (MQ=255)

     aaGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTa                                                                                                                                                  >  1:2914331/1‑150 (MQ=255)

     aaGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTAACCCAAGGTGATTATAGAAATTTCACTTTCCACTa                                                                                                                                                  >  2:1633157/1‑150 (MQ=255)

        cGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCa                                                                                                                                               >  1:2128911/1‑150 (MQ=255)

            tAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCactac                                                                                                                                           >  2:2056559/1‑150 (MQ=255)

             aGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCactact                                                                                                                                          >  1:70708/1‑150 (MQ=255)

               aaaTATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCAACTATCACTACTAt                                                                                                                                        >  1:3360867/1‑150 (MQ=255)

                    tgatgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTAACTTTCCACTATCACTACTATGGGGa                                                                                                                                   <  2:2034559/150‑1 (MQ=255)

                     gatgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGAc                                                                                                                                  >  2:1022573/1‑150 (MQ=255)

                      atgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACt                                                                                                                                 >  1:3330357/1‑150 (MQ=255)

                      atgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACt                                                                                                                                 >  2:2751699/1‑150 (MQ=255)

                      atgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACt                                                                                                                                 <  2:1811467/150‑1 (MQ=255)

                       tgGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTc                                                                                                                                <  1:941083/150‑1 (MQ=255)

                            cATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCaa                                                                                                                           >  1:2274272/1‑150 (MQ=255)

                              tatTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAgg                                                                                                                         >  2:3398474/1‑150 (MQ=255)

                                ttAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGAc                                                                                                                       >  2:126478/1‑150 (MQ=255)

                                ttAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGAc                                                                                                                       >  1:1992191/1‑150 (MQ=255)

                                ttAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGAc                                                                                                                       <  1:2918348/150‑1 (MQ=255)

                                 tAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACa                                                                                                                      >  1:628327/1‑150 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACAt                                                                                                                     >  2:89605/1‑150 (MQ=255)

                                  aaGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACAt                                                                                                                     >  1:2606192/1‑150 (MQ=255)

                                    gTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATAc                                                                                                                   >  1:287562/1‑150 (MQ=255)

                                       cTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGACATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGc                                                                                                                >  2:3168363/1‑150 (MQ=255)

                                        tCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGcc                                                                                                               >  2:832404/1‑150 (MQ=255)

                                         cATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCa                                                                                                              >  1:3806308/1‑150 (MQ=255)

                                               aTTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTatat                                                                                                        >  1:1908389/1‑150 (MQ=255)

                                               aTTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTatat                                                                                                        >  2:4018081/1‑150 (MQ=255)

                                                  cTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTc                                                                                                     >  2:3735154/1‑150 (MQ=255)

                                                      aGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCcaca                                                                                                 >  2:2825446/1‑150 (MQ=255)

                                                         tACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGACCGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAAcc                                                                                              >  1:877914/1‑150 (MQ=255)

                                                             aaTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGAt                                                                                          <  2:2904714/150‑1 (MQ=255)

                                                             aaTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGAt                                                                                          <  2:3032928/150‑1 (MQ=255)

                                                              aTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATg                                                                                         <  1:2814421/150‑1 (MQ=255)

                                                              aTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATg                                                                                         >  2:3950600/1‑150 (MQ=255)

                                                              aTGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATg                                                                                         <  1:1707630/150‑1 (MQ=255)

                                                               tGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATgg                                                                                        >  1:1266604/1‑150 (MQ=255)

                                                               tGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATgg                                                                                        >  2:3459863/1‑150 (MQ=255)

                                                                  cAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTc                                                                                     >  1:683569/1‑150 (MQ=255)

                                                                   aaTGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCg                                                                                    >  1:3155240/1‑150 (MQ=255)

                                                                   aaTGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCg                                                                                    >  2:119276/1‑150 (MQ=255)

                                                                    aTGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGa                                                                                   <  1:2390527/150‑1 (MQ=255)

                                                                         aaaaTGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAAc                                                                              <  1:2982993/150‑1 (MQ=255)

                                                                          aaaTGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAAcc                                                                             <  2:3520185/150‑1 (MQ=255)

                                                                              gAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTa                                                                         >  2:2349671/1‑150 (MQ=255)

                                                                              gAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACAGTTa                                                                         <  1:3647155/150‑1 (MQ=255)

                                                                               aaGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTAt                                                                        <  2:3109686/150‑1 (MQ=255)

                                                                                 gTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGa                                                                      <  1:3625992/150‑1 (MQ=255)

                                                                                 gTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCTACTAACCGTTATGa                                                                      >  2:3249681/1‑150 (MQ=255)

                                                                                   tttACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAg                                                                    <  1:1383894/150‑1 (MQ=255)

                                                                                     tACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAggg                                                                  <  1:2237614/150‑1 (MQ=255)

                                                                                      aCATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGa                                                                 <  1:1481294/150‑1 (MQ=255)

                                                                                        aTGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAg                                                               >  1:1188144/1‑150 (MQ=255)

                                                                                         tGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGt                                                              <  2:2125623/150‑1 (MQ=255)

                                                                                         tGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGt                                                              >  2:1090054/1‑150 (MQ=255)

                                                                                         tGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGt                                                              <  1:4043403/150‑1 (MQ=255)

                                                                                           aataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTcc                                                            <  1:1645778/150‑1 (MQ=255)

                                                                                           aataatCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTcc                                                            >  1:253077/1‑150 (MQ=255)

                                                                                             taatCCTGTAGGGGAACGAGCGGATTCACCGATGTTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCtt                                                          <  1:450898/150‑1 (MQ=255)

                                                                                                  cTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTgaaa                                                     >  1:2733056/1‑150 (MQ=255)

                                                                                                     tAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTGATGAAGGGAAGTCCTTTgaaagaa                                                  >  1:1589996/1‑150 (MQ=255)

                                                                                                        gggAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGaaagaaaag                                               >  2:403858/1‑150 (MQ=255)

                                                                                                           aaCGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATg                                            >  1:907431/1‑150 (MQ=255)

                                                                                                            aCGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATga                                           <  1:1848517/150‑1 (MQ=255)

                                                                                                              gAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATgaga                                         >  2:3700280/1‑150 (MQ=255)

                                                                                                                  ggATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTc                                     >  1:1613882/1‑150 (MQ=255)

                                                                                                                   gATTCACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCa                                    >  1:3633091/1‑150 (MQ=255)

                                                                                                                    aTTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAt                                   >  2:3844936/1‑150 (MQ=255)

                                                                                                                    aTTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAt                                   >  1:1660575/1‑150 (MQ=255)

                                                                                                                    aTTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAt                                   <  2:774585/150‑1 (MQ=255)

                                                                                                                    aTTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAt                                   <  2:468573/150‑1 (MQ=255)

                                                                                                                     ttCACCCAAGGTGATTATATAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTACTTTGAAAGAAAAGATGAGAGTTCAtt                                  >  2:1454650/1‑150 (MQ=255)

                                                                                                                     ttCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAtt                                  <  1:1043516/150‑1 (MQ=255)

                                                                                                                     ttCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCAtt                                  >  2:4159159/1‑150 (MQ=255)

                                                                                                                       cACCCAAGGTGATTATAGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTac                                >  2:397990/1‑150 (MQ=255)

                                                                                                                          ccAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACAcc                             >  1:3006504/1‑150 (MQ=255)

                                                                                                                             aGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGa                          <  2:177853/150‑1 (MQ=255)

                                                                                                                                    tataGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTaa                   >  2:1694862/1‑150 (MQ=255)

                                                                                                                                    tataGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTaa                   <  2:1528961/150‑1 (MQ=255)

                                                                                                                                    tataGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTaa                   >  1:44255/1‑150 (MQ=255)

                                                                                                                                     ataGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAAc                  <  1:1137926/150‑1 (MQ=255)

                                                                                                                                     ataGAAATTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAAc                  <  1:3963145/150‑1 (MQ=255)

                                                                                                                                     ataGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAAc                  >  2:2985721/1‑150 (MQ=255)

                                                                                                                                         aaaTTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAgg              <  2:70708/150‑1 (MQ=255)

                                                                                                                                         aaaTTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAgg              <  1:355261/150‑1 (MQ=255)

                                                                                                                                          aaTTTCACTTTTCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGt             >  2:2198241/1‑150 (MQ=255)

                                                                                                                                            tttCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTc           <  1:1002181/150‑1 (MQ=255)

                                                                                                                                             ttCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCt          <  1:3866427/150‑1 (MQ=255)

                                                                                                                                              tCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTg         <  2:2435168/150‑1 (MQ=255)

                                                                                                                                              tCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTg         <  2:2550494/150‑1 (MQ=255)

                                                                                                                                              tCAATTTCCACTATCTCTTCTCTGGGGACTCGTCAAGGACATACATCCTTTATATTTCCTCTACGTGATTGTTGGACTAAGGGTTTTGATGTGAATTACTTTGAAATAAATGTTTAGTGTTTTTTTCACATTATTATTATCTAGGTTATg         <  2:3614502/150‑1 (MQ=255)

                                                                                                                                                aCTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGc       >  2:2071585/1‑150 (MQ=255)

                                                                                                                                                  tttCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgt     >  2:504292/1‑150 (MQ=255)

                                                                                                                                                   ttCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtg    <  2:1367617/150‑1 (MQ=255)

                                                                                                                                                    tCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCgtgt   <  1:2056559/150‑1 (MQ=255)

CAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCTCATCTTATTCTAAAGATACTAATGGCAATGAAAAAATGAAGTTTTACATGAATAATCCTGTAGGGGAACGAGCGGATTCACCCAAGGTGATTATAGAAATTTCACTTTCCACTATCACTACTATGGGGACTCGTCAAGGACATACAGCCATTATATTTCCACAACCTGATGGTTCGACTAACCGTTATGAAGGGAAGTCCTTTGAAAGAAAAGATGAGAGTTCATTACACCTGATTACTAACAAGGTTCTGGCGTGTT  >  CP009273/4465475‑4465773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`