BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,494,721	G→A	51.6%	A283T (GCC→ACC)	yjhB →	putative transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,494,721	0	G	A	51.6%	‑0.9 / inf	287	A283T (GCC→ACC)	yjhB	putative transporter
Reads supporting (aligned to +/- strand): ref base G (55/84); new base A (67/81); total (122/165)
Fisher's exact test for biased strand distribution p-value = 3.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.60e-01

Only 100 of 288 total aligned reads displayed.

GCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATA  >  CP009273/4494573‑4494869

 ccGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGGTTGGTGTAAAAAAAGcc                                                                                                                                                    >  2:3823035/1‑150 (MQ=255)

 ccGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGcc                                                                                                                                                    >  1:828775/1‑150 (MQ=255)

     tAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTg                                                                                                                                                <  1:3769559/150‑1 (MQ=255)

      aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGt                                                                                                                                               >  2:2966222/1‑150 (MQ=255)

      aaaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGt                                                                                                                                               >  1:2504544/1‑150 (MQ=255)

       aaCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGAGTGGTGTAAAAAAAGCCTTTGTa                                                                                                                                              >  2:3961894/1‑150 (MQ=255)

        aCGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAg                                                                                                                                             <  1:2726256/150‑1 (MQ=255)

         cGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGt                                                                                                                                            <  2:3417272/150‑1 (MQ=255)

          ggACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTc                                                                                                                                           <  2:2295408/150‑1 (MQ=255)

           gACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCg                                                                                                                                          <  1:3988772/150‑1 (MQ=255)

                cttccttcCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCta                                                                                                                                     <  2:2908256/150‑1 (MQ=255)

                 ttccttccTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCtaa                                                                                                                                    >  1:3212085/1‑150 (MQ=255)

                  tccttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCtaat                                                                                                                                   <  1:766298/150‑1 (MQ=255)

                  tcctacctACCAGGCAGATAATGGAGTTAAAACAGTGGTCATTACAACTCTGATGAAAATAGAAGTTTTAGGAACACTGACAGGTACAATATTTTTTATTTTTGTTGGTGATAAGATTGGAGTAAAAAAAGCCTTTCTATTCGGTCtaat                                                                                                                                   >  2:3702070/1‑150 (MQ=255)

                    cttcctACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCtaataa                                                                                                                                 >  1:1487762/1‑150 (MQ=255)

                         tACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCa                                                                                                                            <  1:710513/150‑1 (MQ=255)

                              ggaaGATAATGGAGTTAATACATTTGTCAATTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTTGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTACatttat                                                                                                                       <  2:4111076/147‑1 (MQ=255)

                                 aGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTAtttt                                                                                                                    <  2:904004/150‑1 (MQ=255)

                                  gATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTc                                                                                                                   >  2:3356598/1‑150 (MQ=255)

                                   aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                  >  2:2546653/1‑150 (MQ=255)

                                   aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                  >  1:3048041/1‑150 (MQ=255)

                                   aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                  <  1:3007829/150‑1 (MQ=255)

                                   aTAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTcc                                                                                                                  >  1:1104758/1‑150 (MQ=255)

                                      aTGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCttt                                                                                                               <  2:1873428/150‑1 (MQ=255)

                                       tGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTg                                                                                                              <  1:1809745/150‑1 (MQ=255)

                                           tttAATTCAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCct                                                                                                          <  1:3011047/149‑1 (MQ=255)

                                             tAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCctct                                                                                                        >  1:1010895/1‑150 (MQ=255)

                                              aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                       >  1:521874/1‑150 (MQ=255)

                                              aaTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtt                                                                                                       <  1:3173409/150‑1 (MQ=255)

                                               aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTTGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                      <  1:1839955/150‑1 (MQ=255)

                                               aTACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCttt                                                                                                      <  1:1526655/150‑1 (MQ=255)

                                                  cAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCtttttt                                                                                                   <  2:3583856/150‑1 (MQ=255)

                                                     tGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTa                                                                                                >  2:766711/1‑150 (MQ=255)

                                                      ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                               >  2:2605425/1‑150 (MQ=255)

                                                      ggTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAt                                                                                               <  2:1547940/150‑1 (MQ=255)

                                                       gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtt                                                                                              >  1:2066473/1‑150 (MQ=255)

                                                       gTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTAtt                                                                                              <  1:307186/150‑1 (MQ=255)

                                                         cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                            <  1:226425/150‑1 (MQ=255)

                                                         cATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTc                                                                                            >  1:195450/1‑150 (MQ=255)

                                                          aTTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCt                                                                                           >  1:3375131/1‑150 (MQ=255)

                                                               aaCTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGaa                                                                                      >  2:194337/1‑150 (MQ=255)

                                                                   ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                  <  1:3882091/150‑1 (MQ=255)

                                                                   ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                  >  2:3094131/1‑150 (MQ=255)

                                                                   ctGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAAc                                                                                  >  2:2783983/1‑150 (MQ=255)

                                                                      atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                               <  1:3340833/150‑1 (MQ=255)

                                                                      atgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActct                                                                               <  1:1248148/150‑1 (MQ=255)

                                                                       tgaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActctt                                                                              <  1:2974110/150‑1 (MQ=255)

                                                                        gaCAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttc                                                                             >  2:2099786/1‑150 (MQ=255)

                                                                          cAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctc                                                                           <  1:923392/150‑1 (MQ=255)

                                                                           aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                          <  2:2190279/150‑1 (MQ=255)

                                                                           aaTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctct                                                                          <  2:2761157/150‑1 (MQ=255)

                                                                            aTAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAActcttctctt                                                                         <  1:4028655/150‑1 (MQ=255)

                                                                             tAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTta                                                                        >  2:3095380/1‑150 (MQ=255)

                                                                              aGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTtat                                                                       <  1:2299611/150‑1 (MQ=255)

                                                                                 aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                    <  2:785031/150‑1 (MQ=255)

                                                                                 aGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAgg                                                                    <  2:3061947/150‑1 (MQ=255)

                                                                                  ggTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGa                                                                   <  1:2082939/150‑1 (MQ=255)

                                                                                   gTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGAt                                                                  <  2:3345509/150‑1 (MQ=255)

                                                                                        ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                             >  1:1609737/1‑150 (MQ=255)

                                                                                        ggAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGt                                                             >  1:3732216/1‑150 (MQ=255)

                                                                                         gAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGtc                                                            >  1:3925420/1‑150 (MQ=255)

                                                                                              cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGTTAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                       <  1:1687954/150‑1 (MQ=255)

                                                                                              cTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCttt                                                       <  1:2590277/150‑1 (MQ=255)

                                                                                                gACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTgg                                                     >  1:3352036/1‑150 (MQ=255)

                                                                                                 aCAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGa                                                    <  2:1167195/150‑1 (MQ=255)

                                                                                                 aCAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGa                                                    >  1:755065/1‑150 (MQ=255)

                                                                                                  cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                                   <  1:2645360/150‑1 (MQ=255)

                                                                                                  cAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAt                                                   >  1:668405/1‑150 (MQ=255)

                                                                                                   aGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGAtt                                                  >  2:12940/1‑150 (MQ=255)

                                                                                                    ggTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                                 <  2:47061/150‑1 (MQ=255)

                                                                                                    ggTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTa                                                 <  2:46661/150‑1 (MQ=255)

                                                                                                     gTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTaa                                                >  2:3212024/1‑150 (MQ=255)

                                                                                                      tACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAAt                                               <  1:628908/150‑1 (MQ=255)

                                                                                                       aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                              <  1:712368/150‑1 (MQ=255)

                                                                                                       aCAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATg                                              <  2:1409073/150‑1 (MQ=255)

                                                                                                        cAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATATGATTATGTCTCTTTGGATTAATGt                                             >  2:879725/1‑150 (MQ=255)

                                                                                                         aaTATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGtt                                            >  2:1007383/1‑150 (MQ=255)

                                                                                                            atTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                         >  1:2408823/1‑150 (MQ=255)

                                                                                                            atTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTAc                                         >  2:1461806/1‑150 (MQ=255)

                                                                                                             ttttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTATAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACa                                        <  2:1371178/150‑1 (MQ=255)

                                                                                                               ttttGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCAGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACaaa                                      >  1:2424778/1‑150 (MQ=255)

                                                                                                                  tGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                   <  1:1789599/150‑1 (MQ=255)

                                                                                                                  tGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAAttt                                   <  2:4076141/150‑1 (MQ=255)

                                                                                                                    gTTTTGTTGGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAg                                 >  2:1706081/1‑150 (MQ=255)

                                                                                                                       ttgttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTa                              >  2:1233009/1‑150 (MQ=255)

                                                                                                                         gttgGTGATAAGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTAtt                            >  1:3241235/1‑150 (MQ=255)

                                                                                                                          ttgGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTg                           >  2:2685021/1‑150 (MQ=255)

                                                                                                                                  aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                   >  1:868470/1‑150 (MQ=255)

                                                                                                                                  aaGATTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttg                   <  1:3512764/150‑1 (MQ=255)

                                                                                                                                   aGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttgg                  <  2:2847890/150‑1 (MQ=255)

                                                                                                                                    gATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggt                 <  1:3677041/150‑1 (MQ=255)

                                                                                                                                     aTTGGTGTAAAAAAAACCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGggttggtt                <  1:1580762/150‑1 (MQ=255)

                                                                                                                                       tGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTcc              <  1:1721675/150‑1 (MQ=255)

                                                                                                                                        ggTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCa             <  2:3043767/150‑1 (MQ=255)

                                                                                                                                         gtgtAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCaa            >  2:213427/1‑150 (MQ=255)

                                                                                                                                            tAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAt         <  1:3021369/150‑1 (MQ=255)

                                                                                                                                             aaaaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAtt        >  1:1166605/1‑150 (MQ=255)

                                                                                                                                              aaaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAAttt       <  2:655629/150‑1 (MQ=255)

                                                                                                                                               aaaaaaCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta      <  2:3957076/150‑1 (MQ=255)

                                                                                                                                               aaaaaGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTta      >  1:547500/1‑150 (MQ=255)

GCCGATAAACGGACTACTTCCTTCCTACCTGGCAGATAATGGAGTTAATACAGTGGTCATTTCAACTCTGATGACAATAGCAGGTTTAGGAACACTGACAGGTACAATATTTTTTGGTTTTGTTGGTGATAAGATTGGTGTAAAAAAAGCCTTTGTAGTCGGTCTAATAACTTCATTTATTTTCCTTTGTCCTCTTTTTTTTATTTCTGTGAAAAACTCTTCTCTTATAGGATTATGTCTCTTTGGATTAATGTTTACAAATTTAGGTATTGCAGGGTTGGTTCCAAAATTTATATA  >  CP009273/4494573‑4494869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`