BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,495,349	G→T	48.0%	K82N (AAG→AAT)	yjhC →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,495,349	0	G	T	48.0%	141.7 / inf	256	K82N (AAG→AAT)	yjhC	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base G (70/63); new base T (58/65); total (128/128)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01

Only 100 of 259 total aligned reads displayed.

GATCCTGAAAATGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGT  >  CP009273/4495200‑4495497

  tCCTGAAAATGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGaa                                                                                                                                                    >  1:115404/1‑150 (MQ=255)

    cTGAAAATGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATa                                                                                                                                                  <  2:2666092/150‑1 (MQ=255)

     tGAAAATGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATaa                                                                                                                                                 >  1:1988047/1‑150 (MQ=255)

       aaaaTGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATaaga                                                                                                                                               >  2:3230284/1‑150 (MQ=255)

         aaTGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATaagaag                                                                                                                                             <  1:2505000/150‑1 (MQ=255)

           tGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCa                                                                                                                                           <  1:3296363/150‑1 (MQ=255)

           tGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCa                                                                                                                                           >  2:1106525/1‑150 (MQ=255)

            ggAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCAt                                                                                                                                          <  2:3426119/150‑1 (MQ=255)

             gagaAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATg                                                                                                                                         >  1:3328092/1‑150 (MQ=255)

              agaAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGt                                                                                                                                        <  2:1838146/150‑1 (MQ=255)

                 aaaTATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGtttt                                                                                                                                     >  2:247379/1‑150 (MQ=255)

                  aaTATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGttttt                                                                                                                                    <  2:2018549/150‑1 (MQ=255)

                  aaTATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGttttt                                                                                                                                    <  2:2297831/150‑1 (MQ=255)

                          ccGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGaaaa                                                                                                                            <  2:3353518/150‑1 (MQ=255)

                           cGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGaaaaa                                                                                                                           <  1:1318962/150‑1 (MQ=255)

                            gTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAAc                                                                                                                          <  2:654735/150‑1 (MQ=255)

                            gTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAAc                                                                                                                          >  1:2078884/1‑150 (MQ=255)

                             tGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAAcc                                                                                                                         <  2:625635/150‑1 (MQ=255)

                               aaTTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCaa                                                                                                                       >  2:2830774/1‑150 (MQ=255)

                                 ttGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAAtt                                                                                                                     <  2:3918901/150‑1 (MQ=255)

                                 ttGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGAGTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAAtt                                                                                                                     <  2:3092536/150‑1 (MQ=255)

                                        gtATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTaa                                                                                                              >  2:2266464/1‑150 (MQ=255)

                                          aTCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGt                                                                                                            >  2:419913/1‑150 (MQ=255)

                                          aTCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGt                                                                                                            <  2:1569651/150‑1 (MQ=255)

                                          aTCAATAGGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGt                                                                                                            <  1:3588597/150‑1 (MQ=255)

                                           tCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGtt                                                                                                           <  1:196165/150‑1 (MQ=255)

                                             aaTATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTAt                                                                                                         <  2:1952410/150‑1 (MQ=255)

                                             aaTATGTCAAGCTTGGATGCTATAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTAt                                                                                                         <  1:1003253/150‑1 (MQ=255)

                                              atatGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATg                                                                                                        <  1:3907637/150‑1 (MQ=255)

                                              atatGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATg                                                                                                        <  2:846964/150‑1 (MQ=255)

                                                atGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGaa                                                                                                      <  2:4034041/150‑1 (MQ=255)

                                                 tGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAg                                                                                                     >  2:3054927/1‑150 (MQ=255)

                                                 tGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAg                                                                                                     >  1:3635616/1‑150 (MQ=255)

                                                      aGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATtgt                                                                                                <  1:1869378/150‑1 (MQ=255)

                                                       gCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATtgtg                                                                                               >  1:3850525/1‑150 (MQ=255)

                                                            gATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGTat                                                                                          >  2:468853/1‑150 (MQ=255)

                                                            gATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGat                                                                                          <  2:3184064/150‑1 (MQ=255)

                                                             aTGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGata                                                                                         <  1:2325171/150‑1 (MQ=255)

                                                                cTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATgg                                                                                      <  2:3493252/150‑1 (MQ=255)

                                                                cTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATgg                                                                                      >  1:1200449/1‑150 (MQ=255)

                                                                      tctcAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAg                                                                                >  1:2570182/1‑150 (MQ=255)

                                                                      tctcAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATGAAGTTATGAAGATTGTGTGGATATGGTCAAAg                                                                                >  2:1822158/1‑150 (MQ=255)

                                                                        tcAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCg                                                                              <  1:1716567/150‑1 (MQ=255)

                                                                              aaaTTAGTCGATTGCGTGATCGTAGACACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTCTGAAGAGTGTGTGGATATGGTCAAAGCgttaaaa                                                                        >  2:241226/1‑145 (MQ=255)

                                                                               aaTTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAaag                                                                       >  2:1986809/1‑150 (MQ=255)

                                                                               aaTTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAaag                                                                       <  1:2219333/150‑1 (MQ=255)

                                                                               aaTTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAaag                                                                       >  2:1937715/1‑150 (MQ=255)

                                                                                 ttAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAaagaa                                                                     <  1:2738786/150‑1 (MQ=255)

                                                                                  tAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCAGTAAGTTATGAAGATTGTGTGGATATGGTAAAAGCGTGTAaagaag                                                                    >  2:1234646/1‑150 (MQ=255)

                                                                                  tAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAaagaag                                                                    >  1:4037834/1‑150 (MQ=255)

                                                                                   aGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGc                                                                   >  2:2839559/1‑150 (MQ=255)

                                                                                   aGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGc                                                                   <  2:2149661/150‑1 (MQ=255)

                                                                                    gTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCt                                                                  <  1:94398/150‑1 (MQ=255)

                                                                                       gATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGgt                                                               <  2:3964894/150‑1 (MQ=255)

                                                                                        aTTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGgtg                                                              >  2:3637432/1‑150 (MQ=255)

                                                                                               gATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCtt                                                       <  1:1090457/150‑1 (MQ=255)

                                                                                               gATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCtt                                                       >  1:1225559/1‑150 (MQ=255)

                                                                                                aTCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCttt                                                      <  1:3542832/150‑1 (MQ=255)

                                                                                                 tCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTa                                                     <  2:252391/150‑1 (MQ=255)

                                                                                                    tAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAATCAAATTGCATTATGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATgg                                                  >  1:2236142/1‑150 (MQ=255)

                                                                                                     aGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTGTATGGc                                                 >  1:2940360/1‑150 (MQ=255)

                                                                                                       ccACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCg                                               >  2:2949006/1‑150 (MQ=255)

                                                                                                          ccccAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGc                                            <  1:2195958/150‑1 (MQ=255)

                                                                                                             cAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCata                                         <  1:1608427/150‑1 (MQ=255)

                                                                                                              aaaTTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCAATAAGTTATGAAGAGTGTGTGGATATGGTCAAAGCGTGTAAAGAATCTGGTGTGACCTTTATGGCAGGGCatat                                        >  2:1919743/1‑150 (MQ=255)

                                                                                                                aTTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCAtatta                                      <  2:3580189/150‑1 (MQ=255)

                                                                                                                     cTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAt                                 >  1:3708106/1‑150 (MQ=255)

                                                                                                                     cTGCATAAAGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAt                                 >  1:1936090/1‑150 (MQ=255)

                                                                                                                      tGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAtt                                <  2:297679/150‑1 (MQ=255)

                                                                                                                         aTAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAAttttt                             >  2:1448756/1‑150 (MQ=255)

                                                                                                                           aaaGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTc                           >  2:2569840/1‑150 (MQ=255)

                                                                                                                            aaGAACCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCa                          <  2:1818417/150‑1 (MQ=255)

                                                                                                                            aaGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGAATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCa                          >  2:2083664/1‑150 (MQ=255)

                                                                                                                             aGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCaa                         >  2:3409210/1‑150 (MQ=255)

                                                                                                                              gAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAAt                        >  1:1837441/1‑150 (MQ=255)

                                                                                                                               aaCCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATg                       <  2:660225/150‑1 (MQ=255)

                                                                                                                                aCCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATgg                      <  2:2469480/150‑1 (MQ=255)

                                                                                                                                aCCAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATgg                      >  1:1895442/1‑150 (MQ=255)

                                                                                                                                aCCAGAAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTTCATTAAGTTATGAAGATTGTGTGGATAAGTTCAAAGAGTGTAAAGAAGATGGTATGACCTTTATGTCCGGGCATATTATGAATTTTTTCAAttg                      >  2:3280595/1‑148 (MQ=255)

                                                                                                                                 ccAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATggg                     <  1:889911/150‑1 (MQ=255)

                                                                                                                                  cAGTAATTAAAGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATgggg                    >  1:533256/1‑150 (MQ=255)

                                                                                                                                   aGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGt                   >  1:1524152/1‑150 (MQ=255)

                                                                                                                                   aGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGt                   >  1:145921/1‑150 (MQ=255)

                                                                                                                                        ttAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAat              >  1:2658141/1‑150 (MQ=255)

                                                                                                                                         tAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAata             >  2:4032754/1‑150 (MQ=255)

                                                                                                                                          aaaGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAatat            >  2:1733200/1‑150 (MQ=255)

                                                                                                                                           aaGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATg           >  2:1680369/1‑150 (MQ=255)

                                                                                                                                           aaGCAGCAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATg           >  2:3779545/1‑150 (MQ=255)

                                                                                                                                            agcagcAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGTCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGc          <  2:3198313/150‑1 (MQ=255)

                                                                                                                                            agcagcAAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGc          >  1:494435/1‑150 (MQ=255)

                                                                                                                                            agcagcAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGc          >  1:671413/1‑150 (MQ=255)

                                                                                                                                              cagcaAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCAc        <  2:2531724/150‑1 (MQ=255)

                                                                                                                                               agcaAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACg       >  1:2352380/1‑150 (MQ=255)

                                                                                                                                               agcaAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACg       >  2:756162/1‑150 (MQ=255)

                                                                                                                                               agcaAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCAGATTATGAATTTTTTCAATGGGGTTCAATATGCACg       >  1:2993470/1‑150 (MQ=255)

                                                                                                                                               agcaAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACg       >  2:683102/1‑150 (MQ=255)

                                                                                                                                                gcaAATAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACgg      >  1:2902954/1‑150 (MQ=255)

                                                                                                                                                  aaaTAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGaa    <  1:135520/150‑1 (MQ=255)

                                                                                                                                                    aGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGt  >  1:3458360/1‑150 (MQ=255)

                                                                                                                                                    aGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGt  >  1:1078398/1‑150 (MQ=255)

GATCCTGAAAATGGAGAAAATATTGCCCGTGAATTGCAGTGTATCAATATGTCAAGCTTGGATGCTTTAGTCTCAAGTAAATTAGTCGATTGCGTGATCGTAGCCACCCCAAATTATCTGCATAAAGAACCAGTAATTAAAGCAGCAAAGAATAAGAAGCATGTTTTTTGTGAAAAACCAATTGCATTAAGTTATGAAGATTGTGTGGATATGGTCAAAGCGTGTAAAGAAGCTGGTGTGACCTTTATGGCCGGGCATATTATGAATTTTTTCAATGGGGTTCAATATGCACGGAAGT  >  CP009273/4495200‑4495497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`