BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,572,581	G→T	37.3%	T95T (ACC→ACA)	hsdM ←	DNA methyltransferase M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,572,581	0	G	T	37.3%	310.0 / 226.6	217	T95T (ACC→ACA)	hsdM	DNA methyltransferase M
Reads supporting (aligned to +/- strand): ref base G (61/75); new base T (41/39); total (103/114)
Fisher's exact test for biased strand distribution p-value = 3.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

Only 100 of 221 total aligned reads displayed.

CCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTT  >  CP009273/4572432‑4572729

      ttGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTa                                                                                                                                                >  2:2539799/1‑150 (MQ=255)

       tGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGTAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTTCCAGTCCAGCGTATCCATTTTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTaa                                                                                                                                               <  2:39713/150‑1 (MQ=255)

       tGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTaa                                                                                                                                               <  1:1222865/150‑1 (MQ=255)

        ggTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAAc                                                                                                                                              <  2:2330176/150‑1 (MQ=255)

         gTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACa                                                                                                                                             <  2:5203/150‑1 (MQ=255)

          tttCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACAt                                                                                                                                            <  1:375252/150‑1 (MQ=255)

             cATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTAt                                                                                                                                         >  2:770946/1‑150 (MQ=255)

             cATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTAt                                                                                                                                         <  2:2584219/150‑1 (MQ=255)

              aTTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATg                                                                                                                                        >  2:417859/1‑150 (MQ=255)

               ttCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGa                                                                                                                                       >  1:4161162/1‑150 (MQ=255)

                tCGCGTTCTTCTGCAACAGCCCTTCGTACATATNGCCGAAGTCNTCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGaa                                                                                                                                      <  1:1587345/150‑1 (MQ=255)

                tCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGaa                                                                                                                                      <  1:3693951/150‑1 (MQ=255)

                    gTTCTTCTTCAACAGCCCTTCGTACATATCGCAGAAGTCATCGAGCGACTTACAGTTCGCTCAGTTGTACCAGTCTATCGAATCCATATTTCTGTCCTGTGCTTTTTTTTGTTTCGTCTCGGTGATTGTGGTTCTAACATTATTaaaata                                                                                                                                  <  2:3129937/150‑3 (MQ=255)

                    gTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAAc                                                                                                                                  >  1:1628652/1‑150 (MQ=255)

                    gTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAAc                                                                                                                                  >  2:1327403/1‑150 (MQ=255)

                      tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTg                                                                                                                                >  1:1844370/1‑150 (MQ=255)

                      tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTg                                                                                                                                >  1:725664/1‑150 (MQ=255)

                      tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTg                                                                                                                                >  2:2966860/1‑150 (MQ=255)

                      tcttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTg                                                                                                                                >  1:2816420/1‑150 (MQ=255)

                       cttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGc                                                                                                                               >  1:1520896/1‑150 (MQ=255)

                       cttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGc                                                                                                                               >  2:3395825/1‑150 (MQ=255)

                        ttctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGcc                                                                                                                              >  1:265498/1‑150 (MQ=255)

                         tctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATAGTGCTGACCAGTGAGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCt                                                                                                                             <  1:482301/150‑1 (MQ=255)

                          ctGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTg                                                                                                                            <  1:2503041/150‑1 (MQ=255)

                           tGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCAGATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGAGGGTGGTACTCACATTATGAAAAACTGACTGt                                                                                                                           >  2:1987110/1‑150 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTAcc                                                                                                                        <  2:4090218/150‑1 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTAcc                                                                                                                        >  1:510184/1‑150 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTAcc                                                                                                                        >  1:4128447/1‑150 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTCCTAACAGTATGAAAAACTGCCTGTAcc                                                                                                                        >  2:1284068/1‑150 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTAcc                                                                                                                        >  2:2929703/1‑150 (MQ=255)

                              aaCAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTAcc                                                                                                                        <  2:1092038/150‑1 (MQ=255)

                                 aGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGc                                                                                                                     >  2:840540/1‑150 (MQ=255)

                                 aGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGc                                                                                                                     >  1:2414915/1‑150 (MQ=255)

                                  gCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCt                                                                                                                    >  1:1531042/1‑150 (MQ=255)

                                     cTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCtttt                                                                                                                 <  2:3064210/150‑1 (MQ=255)

                                     cTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCtttt                                                                                                                 >  2:1501295/1‑150 (MQ=255)

                                     cTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCtttt                                                                                                                 >  1:2066850/1‑150 (MQ=255)

                                       tCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTg                                                                                                               <  2:1913273/150‑1 (MQ=255)

                                          tACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCa                                                                                                            <  1:3237761/150‑1 (MQ=255)

                                            cATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATc                                                                                                          <  1:3320430/150‑1 (MQ=255)

                                              tatCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCtt                                                                                                        <  2:1359928/150‑1 (MQ=255)

                                               atCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTc                                                                                                       <  1:1442920/150‑1 (MQ=255)

                                               atCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTc                                                                                                       <  2:2595197/150‑1 (MQ=255)

                                                  gCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGcc                                                                                                    >  2:2014173/1‑150 (MQ=255)

                                                   ccGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCt                                                                                                   >  1:2129030/1‑150 (MQ=255)

                                                      aaGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTaaa                                                                                                >  2:481650/1‑150 (MQ=255)

                                                      aaGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTaaa                                                                                                >  1:2721996/1‑150 (MQ=255)

                                                       aGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAAt                                                                                               >  1:2716374/1‑150 (MQ=255)

                                                       aGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAAt                                                                                               <  2:3559258/150‑1 (MQ=255)

                                                         tCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGc                                                                                             >  2:1693090/1‑150 (MQ=255)

                                                               cgcgACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGc                                                                                       >  2:2727855/1‑150 (MQ=255)

                                                                 cgACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCAt                                                                                     <  2:3254401/150‑1 (MQ=255)

                                                                 cgACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCAt                                                                                     <  1:1935018/150‑1 (MQ=255)

                                                                  gACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCAtt                                                                                    <  2:352012/150‑1 (MQ=255)

                                                                   aCTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCAttt                                                                                   >  2:2158655/1‑150 (MQ=255)

                                                                     ttACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTAACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCAttttt                                                                                 >  2:1612005/1‑150 (MQ=255)

                                                                      tACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTc                                                                                <  1:1906449/150‑1 (MQ=255)

                                                                          gTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTa                                                                            <  1:1493390/150‑1 (MQ=255)

                                                                          gTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTa                                                                            <  1:1284219/150‑1 (MQ=255)

                                                                          gTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTa                                                                            <  1:3214755/150‑1 (MQ=255)

                                                                          gTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTa                                                                            <  1:3895505/150‑1 (MQ=255)

                                                                             cgcgcCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaa                                                                         <  2:3783249/150‑1 (MQ=255)

                                                                              gcgcCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaac                                                                        <  1:3780026/150‑1 (MQ=255)

                                                                               cgcCGTTGTACCATTCCAGCGAATCCCTCTTGCTGTGCAGTGCGGTTATTTGTTTCGGCTAGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTAGCCTAACTGCACGAGCATTTTTCGGTAGaact                                                                       <  1:1445487/150‑1 (MQ=255)

                                                                                  cGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaactgca                                                                    <  1:78099/150‑1 (MQ=255)

                                                                                  cGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGaactgca                                                                    >  1:2419984/1‑150 (MQ=255)

                                                                                          cAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTcc                                                            <  2:3300308/150‑1 (MQ=255)

                                                                                           aGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCt                                                           <  2:3055335/150‑1 (MQ=255)

                                                                                           aGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCt                                                           <  2:1665906/150‑1 (MQ=255)

                                                                                           aGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCt                                                           <  1:2752597/150‑1 (MQ=255)

                                                                                            gTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTg                                                          <  1:909646/150‑1 (MQ=255)

                                                                                             tCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTgg                                                         <  2:199980/150‑1 (MQ=255)

                                                                                             tCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTgg                                                         >  1:1025535/1‑150 (MQ=255)

                                                                                                  cGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGAt                                                    <  1:1270339/150‑1 (MQ=255)

                                                                                                   gAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATg                                                   <  1:2193620/150‑1 (MQ=255)

                                                                                                   gAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATg                                                   >  1:1701010/1‑150 (MQ=255)

                                                                                                     aTCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCg                                                 <  1:3076909/150‑1 (MQ=255)

                                                                                                     aTCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCg                                                 <  1:1073997/150‑1 (MQ=255)

                                                                                                     aTCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCg                                                 <  2:2613875/150‑1 (MQ=255)

                                                                                                         atatTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGAt                                             >  2:2625345/1‑150 (MQ=255)

                                                                                                          tatTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGAtt                                            <  1:1414404/150‑1 (MQ=255)

                                                                                                          tatTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGAtt                                            <  2:2357566/150‑1 (MQ=255)

                                                                                                             tGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCa                                         >  1:3537786/1‑150 (MQ=255)

                                                                                                               cTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAgg                                       >  1:86226/1‑150 (MQ=255)

                                                                                                                 gACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTATCATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTc                                     >  2:328319/1‑150 (MQ=255)

                                                                                                                     aGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGATTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATcc                                 >  2:2520302/1‑150 (MQ=255)

                                                                                                                           gTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCgg                           >  1:2881551/1‑150 (MQ=255)

                                                                                                                            ttATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGt                          >  2:2245655/1‑150 (MQ=255)

                                                                                                                             tATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTa                         <  2:141229/150‑1 (MQ=255)

                                                                                                                              aTTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTaa                        >  2:895413/1‑150 (MQ=255)

                                                                                                                              aTTTGTTTCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTaa                        <  2:3933500/150‑1 (MQ=255)

                                                                                                                              aTTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTaa                        <  1:887669/150‑1 (MQ=255)

                                                                                                                               tttGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAAc                       <  1:2544467/150‑1 (MQ=255)

                                                                                                                                   tttCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTc                   >  1:344076/1‑150 (MQ=255)

                                                                                                                                    ttCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTcc                  <  1:3458320/150‑1 (MQ=255)

                                                                                                                                     tCGGCTCGGTGATGGTTGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCg                 <  2:3893031/150‑1 (MQ=255)

                                                                                                                                      cGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCgg                <  1:303999/150‑1 (MQ=255)

                                                                                                                                              tGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTAtt        >  1:2465473/1‑150 (MQ=255)

                                                                                                                                                aTGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTcc      <  2:1734426/150‑1 (MQ=255)

                                                                                                                                                    tggtACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCtt  >  2:837870/1‑150 (MQ=255)

CCAGACTTGGTTTCATTCGCGTTCTTCTGCAACAGCCCTTCGTACATATCGCCGAAGTCATCGCGCGACTTACCGTGCGCGCCGTTGTACCAGTCCAGCGAATCCATATTGCTGACCAGTGCGGTTATTTGTTTCGGCTCGGTGATGGTGGTACTAACATTATGAAAAACTGCCTGTACCAGCTTTTTGTCATCTTCGCCTAAATGCACGAGCATTTTTCGGTAGAACTGCAACTGCTCCTGGCCGATGCGGGATTTCAGGTCATCCCAGCGGTAACCTTCCGGCAGGTATTCCGCTT  >  CP009273/4572432‑4572729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`