Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,590,074 A→C 31.8% V240G (GTA→GGA)  dnaC ← DNA biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,590,0740AC31.8% 209.9 / 239.9 211V240G (GTA→GGA) dnaCDNA biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (87/57);  new base C (32/35);  total (119/92)
Fisher's exact test for biased strand distribution p-value = 1.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.07e-01

Only 100 of 211 total aligned reads displayed.

GACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCG  >  CP009273/4589927‑4590222
                                                                                                                                                   |                                                                                                                                                    
gACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTAcc                                                                                                                                                    >  2:510178/1‑150 (MQ=255)
     agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCt                                                                                                                                               <  2:625557/150‑1 (MQ=255)
     agcaTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCt                                                                                                                                               <  2:248611/150‑1 (MQ=255)
       caTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTAc                                                                                                                                             <  2:2954153/150‑1 (MQ=255)
        aTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACg                                                                                                                                            <  2:2717294/150‑1 (MQ=255)
        aTGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACg                                                                                                                                            <  2:782562/150‑1 (MQ=255)
         tGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACgg                                                                                                                                           >  2:3317394/1‑150 (MQ=255)
          gCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGt                                                                                                                                          >  1:2874247/1‑150 (MQ=255)
            aCCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAg                                                                                                                                        >  1:2518441/1‑150 (MQ=255)
             ccccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGc                                                                                                                                       <  1:2706160/150‑1 (MQ=255)
              cccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCt                                                                                                                                      <  2:1098466/150‑1 (MQ=255)
              cccGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGATTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCt                                                                                                                                      <  2:1657761/150‑1 (MQ=255)
                cGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTAt                                                                                                                                    <  1:2906068/150‑1 (MQ=255)
                 ggTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATc                                                                                                                                   >  2:801654/1‑150 (MQ=255)
                 ggTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATc                                                                                                                                   >  1:2707938/1‑150 (MQ=255)
                 ggTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATc                                                                                                                                   >  2:3753892/1‑150 (MQ=255)
                  gTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATcc                                                                                                                                  <  2:2661544/150‑1 (MQ=255)
                      aaaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGt                                                                                                                              >  2:3118562/1‑150 (MQ=255)
                      aaaTTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGt                                                                                                                              >  2:317712/1‑150 (MQ=255)
                         ttAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGa                                                                                                                           >  2:424687/1‑150 (MQ=255)
                          tAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGaa                                                                                                                          <  2:1874509/150‑1 (MQ=255)
                           aaTGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAg                                                                                                                         >  2:4033686/1‑150 (MQ=255)
                            aTGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGa                                                                                                                        <  2:1155732/150‑1 (MQ=255)
                               cgcTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGACACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCa                                                                                                                     >  2:2570266/1‑150 (MQ=255)
                                gcTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCAc                                                                                                                    <  1:3194817/150‑1 (MQ=255)
                                  tGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGACTTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCAccc                                                                                                                  >  2:2026370/1‑150 (MQ=255)
                                   ggcggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCca                                                                                                                 <  2:236601/150‑1 (MQ=255)
                                    gcggcAATGGCACAGCACAGAAGAGGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAAGTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCcac                                                                                                                <  2:2127627/150‑1 (MQ=255)
                                      ggcAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACaa                                                                                                              >  1:86562/1‑150 (MQ=255)
                                         aaTGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACt                                                                                                           >  2:101864/1‑150 (MQ=255)
                                          aTGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTg                                                                                                          >  2:1355596/1‑150 (MQ=255)
                                            ggCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAATGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGtt                                                                                                        <  2:3698240/150‑1 (MQ=255)
                                              cacagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAc                                                                                                      <  1:2372540/150‑1 (MQ=255)
                                               acagcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTAcc                                                                                                     <  2:3033191/150‑1 (MQ=255)
                                                 agcacagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCa                                                                                                   >  2:2928701/1‑150 (MQ=255)
                                                    acagAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGgc                                                                                                >  2:2796944/1‑150 (MQ=255)
                                                       gaagaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCa                                                                                             >  2:586125/1‑150 (MQ=255)
                                                        aagaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCAt                                                                                            >  1:80768/1‑150 (MQ=255)
                                                         agaTGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATa                                                                                           >  1:437962/1‑150 (MQ=255)
                                                            tGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACgg                                                                                        >  2:1437072/1‑150 (MQ=255)
                                                            tGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACgg                                                                                        >  1:1388619/1‑150 (MQ=255)
                                                               ttAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTcc                                                                                     >  1:3267096/1‑150 (MQ=255)
                                                                tAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCa                                                                                    >  2:1860991/1‑150 (MQ=255)
                                                                 aaCAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCAt                                                                                   >  2:2358329/1‑150 (MQ=255)
                                                                    aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAc                                                                                >  1:2665606/1‑150 (MQ=255)
                                                                    aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAc                                                                                >  2:162113/1‑150 (MQ=255)
                                                                    aGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCAc                                                                                >  2:1210820/1‑150 (MQ=255)
                                                                        ttCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGt                                                                            >  1:3076930/1‑150 (MQ=255)
                                                                         tcatcatcaTTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGtt                                                                           <  2:3935803/150‑1 (MQ=255)
                                                                           atcatcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCg                                                                         <  2:3144961/150‑1 (MQ=255)
                                                                             catcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGcc                                                                       >  1:3501150/1‑150 (MQ=255)
                                                                              atcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGccc                                                                      >  2:1782989/1‑150 (MQ=255)
                                                                               tcatTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGANACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCNATCACGCGTTCGcccn                                                                     <  2:1261040/150‑2 (MQ=255)
                                                                                  ttACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCa                                                                  <  1:3756066/150‑1 (MQ=255)
                                                                                   tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCaa                                                                 <  1:138982/150‑1 (MQ=255)
                                                                                   tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCaa                                                                 >  1:1256405/1‑150 (MQ=255)
                                                                                   tACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCaa                                                                 >  1:2600545/1‑150 (MQ=255)
                                                                                      tCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACtt                                                              <  1:341600/150‑1 (MQ=255)
                                                                                      tCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACtt                                                              <  2:2000931/150‑1 (MQ=255)
                                                                                      tCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACtt                                                              <  1:3808468/150‑1 (MQ=255)
                                                                                         aGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGt                                                           >  2:2991370/1‑150 (MQ=255)
                                                                                              gAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtct                                                      >  1:398088/1‑150 (MQ=255)
                                                                                              gAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCAtct                                                      >  1:937433/1‑150 (MQ=255)
                                                                                                 ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCtt                                                   <  1:2262619/150‑1 (MQ=255)
                                                                                                 ttGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCtt                                                   <  2:1733692/150‑1 (MQ=255)
                                                                                                  tgtgTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTc                                                  <  2:1943956/150‑1 (MQ=255)
                                                                                                      tCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCata                                              <  1:335193/150‑1 (MQ=255)
                                                                                                       cGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                             <  2:2044516/150‑1 (MQ=255)
                                                                                                       cGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCatat                                             <  2:102239/150‑1 (MQ=255)
                                                                                                         cAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTa                                           >  1:567195/1‑150 (MQ=255)
                                                                                                          aGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                          >  2:2198562/1‑150 (MQ=255)
                                                                                                          aGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                          >  2:3858858/1‑150 (MQ=255)
                                                                                                          aGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTAc                                          >  1:3550637/1‑150 (MQ=255)
                                                                                                           gTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCATGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTACCATATTACt                                         >  1:3297207/1‑150 (MQ=255)
                                                                                                           gTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACt                                         >  1:1560317/1‑150 (MQ=255)
                                                                                                           gTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACt                                         >  1:2414193/1‑150 (MQ=255)
                                                                                                              taCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGtt                                      <  2:3657188/150‑1 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTg                                     >  2:41443/1‑150 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTg                                     >  1:2409055/1‑150 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTg                                     <  2:2490417/150‑1 (MQ=255)
                                                                                                               aCCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTg                                     <  2:2490460/150‑1 (MQ=255)
                                                                                                                   ccAGAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCa                                 <  1:23959/150‑1 (MQ=255)
                                                                                                                      gAAACGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCa                              >  1:1409524/1‑150 (MQ=255)
                                                                                                                       aaaCGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCAt                             <  2:1276057/150‑1 (MQ=255)
                                                                                                                        aaCGCGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATc                            >  2:353776/1‑150 (MQ=255)
                                                                                                                          cGGGGATTAATACTCTTTACCTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATccc                          <  1:2995546/150‑1 (MQ=255)
                                                                                                                          cGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAACCTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATccc                          <  2:834935/150‑1 (MQ=255)
                                                                                                                          cGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATccc                          <  2:1232412/150‑1 (MQ=255)
                                                                                                                           ggggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCg                         >  1:3841657/1‑150 (MQ=255)
                                                                                                                           ggggATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCg                         <  1:536316/150‑1 (MQ=255)
                                                                                                                                ttAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTgg                    >  1:1663377/1‑150 (MQ=255)
                                                                                                                                 tAATACTCTTTACCTGTTACCCGGCTACGGTCGCAAACCAATTTGAAGAACACCAACAAACTGTTACCCAGTCGCAAACGGTCCATCACGCGTTCGCCCAGCAAATTGGTCAACTCTTCCATATTACAGTAGGTCAGCATCACGGTTggg                   >  2:1536589/1‑150 (MQ=255)
                                                                                                                                   aTACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCg                 <  1:3703812/150‑1 (MQ=255)
                                                                                                                                    tACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGt                <  1:1989627/150‑1 (MQ=255)
                                                                                                                                             ccTGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaagaa       >  2:2372330/1‑150 (MQ=255)
                                                                                                                                             ccTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGgaagaa       <  1:2251106/150‑1 (MQ=255)
                                                                                                                                               tGTTCCCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAagaaga     <  2:2476655/150‑1 (MQ=255)
                                                                                                                                                gTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAagag    >  1:2923815/1‑150 (MQ=255)
                                                                                                                                                 ttACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgc   >  2:2410617/1‑150 (MQ=255)
                                                                                                                                                  tACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAgcg  >  1:2318037/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GACGCAGCATGCACCCCGGTGGAAATTAATGCGCTGGCGGCAATGGCACAGCACAGAAGATGTTTAACAGTTTTCATCATCATTACTCAAGGTGGAATTGTGTCGCAGTATACCGCCAGAAACGGGGATTAATACTCTTTACCTGTTACCCGGCTACGGTAGCTATCCCAGTTGAAGATCACCCACAAACTGTTACCCAGGCGCATACGGTCCATCACGCGTTCGCCCAGCAACTTGGTCATCTCTTCCATATTACTGTTGGTCAGCATCCCGGTTGGGCGTTTGGAAGAAGAGCG  >  CP009273/4589927‑4590222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑