BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	4,612,781	C→A	7.5%	A399E (GCG→GAG)	yjjJ →	putative protein kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	4,612,781	0	C	A	7.5%	1074.6 / 39.5	279	A399E (GCG→GAG)	yjjJ	putative protein kinase
Reads supporting (aligned to +/- strand): ref base C (133/125); new base A (15/6); total (148/131)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 280 total aligned reads displayed.

TTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCC  >  CP009273/4612634‑4612930

tttatttaTCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGa                                                                                                                                                     >  2:93484/1‑150 (MQ=255)

   atttatCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATcc                                                                                                                                                  >  2:1856052/1‑150 (MQ=255)

   atttatCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATcc                                                                                                                                                  >  1:2424334/1‑150 (MQ=255)

   atttatCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATcc                                                                                                                                                  >  1:2925335/1‑150 (MQ=255)

    tttatCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATccc                                                                                                                                                 <  2:3901646/150‑1 (MQ=255)

     ttatCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCg                                                                                                                                                >  1:1631286/1‑150 (MQ=255)

       atCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCt                                                                                                                                              >  1:2845240/1‑150 (MQ=255)

        tCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTg                                                                                                                                             <  1:2721537/150‑1 (MQ=255)

          tGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGgc                                                                                                                                           <  2:4073284/150‑1 (MQ=255)

          tGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGgc                                                                                                                                           <  1:2867392/150‑1 (MQ=255)

            aaCCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGgcgc                                                                                                                                         >  2:3016084/1‑150 (MQ=255)

                     ttGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCt                                                                                                                                >  1:3267696/1‑150 (MQ=255)

                     ttGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCt                                                                                                                                <  1:2673629/150‑1 (MQ=255)

                      tGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTg                                                                                                                               <  2:1545391/150‑1 (MQ=255)

                      tGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTg                                                                                                                               <  1:237596/150‑1 (MQ=255)

                               gCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGt                                                                                                                      <  2:1994411/150‑1 (MQ=255)

                               gCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGt                                                                                                                      >  2:2622960/1‑150 (MQ=255)

                                   gTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGcc                                                                                                                  >  2:3798890/1‑150 (MQ=255)

                                   gTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGcc                                                                                                                  >  1:257226/1‑150 (MQ=255)

                                      tACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCaga                                                                                                               <  1:3364483/150‑1 (MQ=255)

                                      tACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCaga                                                                                                               <  1:790936/150‑1 (MQ=255)

                                         gACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGAt                                                                                                            >  2:1530124/1‑150 (MQ=255)

                                         gACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGAt                                                                                                            >  2:1698935/1‑150 (MQ=255)

                                         gACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGAt                                                                                                            >  1:1671677/1‑150 (MQ=255)

                                          aCATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATc                                                                                                           >  2:1060262/1‑150 (MQ=255)

                                            aTGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCcg                                                                                                         <  1:2034295/150‑1 (MQ=255)

                                             tgctgcCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCcgc                                                                                                        <  1:1054666/150‑1 (MQ=255)

                                             tgctgcCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCcgc                                                                                                        <  1:4056382/150‑1 (MQ=255)

                                               ctgcCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGt                                                                                                      >  1:696762/1‑150 (MQ=255)

                                                tgcCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTa                                                                                                     >  2:2104313/1‑150 (MQ=255)

                                                   cGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGACAGAGATCCGCGTATCa                                                                                                  <  1:1974603/150‑1 (MQ=255)

                                                    gATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCac                                                                                                 <  1:2173583/150‑2 (MQ=255)

                                                    gATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAg                                                                                                 >  1:768196/1‑150 (MQ=255)

                                                        gTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAg                                                                                             >  2:2545805/1‑150 (MQ=255)

                                                         tCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAgg                                                                                            >  1:2267664/1‑150 (MQ=255)

                                                            aTGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGt                                                                                         <  1:1757333/150‑1 (MQ=255)

                                                            aTGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGt                                                                                         <  2:1634741/150‑1 (MQ=255)

                                                               cACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTc                                                                                      <  1:1799814/150‑1 (MQ=255)

                                                               cACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTc                                                                                      <  1:1836070/150‑1 (MQ=255)

                                                                aCCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCg                                                                                     >  1:3723025/1‑150 (MQ=255)

                                                                aCCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCg                                                                                     >  2:3732685/1‑150 (MQ=255)

                                                                      cAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACAt                                                                               >  2:1168185/1‑150 (MQ=255)

                                                                      cAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACAt                                                                               <  1:1165321/150‑1 (MQ=255)

                                                                       aGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACAtt                                                                              <  1:3474090/150‑1 (MQ=255)

                                                                          gcTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTgcg                                                                           <  1:73727/150‑1 (MQ=255)

                                                                          gcTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTgcg                                                                           <  1:3575048/150‑1 (MQ=255)

                                                                          gcTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTgcg                                                                           >  2:2955815/1‑150 (MQ=255)

                                                                            tGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCa                                                                         >  1:3324935/1‑150 (MQ=255)

                                                                              gAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAg                                                                       >  1:3926290/1‑150 (MQ=255)

                                                                                aTGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGaa                                                                     >  2:502962/1‑150 (MQ=255)

                                                                                  gctgcGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAAt                                                                   >  2:3777538/1‑150 (MQ=255)

                                                                                   ctgcGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTCTCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATg                                                                  >  2:2795767/1‑150 (MQ=255)

                                                                                   ctgcGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATg                                                                  >  2:2287799/1‑150 (MQ=255)

                                                                                    tgcGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGc                                                                 <  2:1200124/150‑1 (MQ=255)

                                                                                      cGTGATGCTGCCATTGAGGTGTAGTTTGATCTTATCGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCg                                                               <  2:3204478/150‑1 (MQ=255)

                                                                                         gATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGaa                                                            <  2:405059/150‑1 (MQ=255)

                                                                                             ctgcCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGaaaaaa                                                        >  2:3535121/1‑150 (MQ=255)

                                                                                             ctgcCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGaaaaaa                                                        <  1:3515046/150‑1 (MQ=255)

                                                                                                 cATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCg                                                    <  2:3944865/150‑1 (MQ=255)

                                                                                                   ttGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGc                                                  <  2:3829632/150‑1 (MQ=255)

                                                                                                    tGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCa                                                 <  1:2718258/150‑1 (MQ=255)

                                                                                                    tGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCa                                                 <  1:3237462/150‑1 (MQ=255)

                                                                                                     gAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCaa                                                <  2:3657949/150‑1 (MQ=255)

                                                                                                        gTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATc                                             <  2:1512778/150‑1 (MQ=255)

                                                                                                         tGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCg                                            <  2:4121436/150‑1 (MQ=255)

                                                                                                            aGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCATCGAGGCGTTTCGCCACATTTCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAag                                         >  1:2505022/1‑150 (MQ=255)

                                                                                                            aGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAag                                         >  2:644572/1‑150 (MQ=255)

                                                                                                              tttGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAagag                                       >  1:1189173/1‑150 (MQ=255)

                                                                                                              tttGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAagag                                       >  1:935854/1‑150 (MQ=255)

                                                                                                              tttGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAagag                                       >  1:3903172/1‑150 (MQ=255)

                                                                                                                 gATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGaaa                                    >  1:1646207/1‑150 (MQ=255)

                                                                                                                 gATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGaaa                                    <  2:1245497/150‑1 (MQ=255)

                                                                                                                 gATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGaaa                                    >  1:178145/1‑150 (MQ=255)

                                                                                                                  aTCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAg                                   >  2:377786/1‑150 (MQ=255)

                                                                                                                  aTCTTAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAg                                   >  2:879210/1‑150 (MQ=255)

                                                                                                                   tCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGt                                  >  2:2090792/1‑150 (MQ=255)

                                                                                                                    cTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGtt                                 >  2:2310368/1‑150 (MQ=255)

                                                                                                                    cTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGtt                                 >  1:4089006/1‑150 (MQ=255)

                                                                                                                      tAACGTCAGTAAAAGCGCTTGGTTAACGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGc                               >  1:3061078/1‑150 (MQ=255)

                                                                                                                        aCGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGccc                             <  1:1886429/150‑1 (MQ=255)

                                                                                                                         tgTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCg                            >  2:452724/2‑150 (MQ=255)

                                                                                                                         cGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCg                            <  1:1749610/150‑1 (MQ=255)

                                                                                                                           tCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCa                          >  2:4035108/1‑150 (MQ=255)

                                                                                                                            cAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCAt                         <  1:1086473/150‑1 (MQ=255)

                                                                                                                                aaaaGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGc                     >  2:3866238/1‑150 (MQ=255)

                                                                                                                                aaaaGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGc                     >  2:3331822/1‑150 (MQ=255)

                                                                                                                                aaaaGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGc                     >  2:2851524/1‑150 (MQ=255)

                                                                                                                                 aaaGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCCCATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCg                    >  2:699781/1‑150 (MQ=255)

                                                                                                                                 aaaGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCg                    >  1:3950401/1‑150 (MQ=255)

                                                                                                                                    gcgcTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGt                 <  2:596671/150‑1 (MQ=255)

                                                                                                                                     cgcTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTa                <  1:3507635/150‑1 (MQ=255)

                                                                                                                                          ggTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTAc           >  1:3012882/1‑150 (MQ=255)

                                                                                                                                          ggTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTAc           >  2:1310342/1‑150 (MQ=255)

                                                                                                                                             tAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCtt        >  1:3829564/1‑150 (MQ=255)

                                                                                                                                              aaCGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACCTTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTa       >  1:48443/1‑150 (MQ=255)

                                                                                                                                               aCGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAACAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTAc      <  2:244954/150‑1 (MQ=255)

                                                                                                                                               aCGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTAc      <  1:1382625/150‑1 (MQ=255)

                                                                                                                                                cGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACa     <  2:3262906/150‑1 (MQ=255)

                                                                                                                                                cGGAGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGACAAAATCCGGCAAATCGAAGAGACAGTTGCCCGCATGGGCGGGTAACTACCTTACa     >  2:1456152/1‑150 (MQ=255)

                                                                                                                                                 ggCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACAGTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAg    >  2:1419113/1‑150 (MQ=255)

TTTATTTATCTGAACCGCCATTTGCGCTGACGCCCGTCTACGACATGCTGCCGATGGTCTATGCACCAAACAGCGCTGGAATGCTGCGTGATGCTGCCATTGAGGTGAAGTTTGATCTTAACGTCAGTAAAAGCGCTTGGTTAACGGCGATCCCGCTGGCGCAGCAGTTCTGGCAAACGGTCGCCAGAGATCCGCGTATCAGCGAGGCGTTTCGCCACATTGCGCAAGAAATGCCGGAAAAAATCCGGCAAATCGAAGAGAAAGTTGCCCGCATGGGCGGGTAACTACCTTACAGCC  >  CP009273/4612634‑4612930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`