Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 49,840 G→T 100% A6A (GCG→GCT)  folA → dihydrofolate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927349,8400GT99.2% 843.0 / ‑1.3 238A6A (GCG→GCT) folAdihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (132/104);  total (133/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

GGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTT  >  CP009273/49692‑49983
                                                                                                                                                    |                                                                                                                                               
ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg                                                                                                                                                <  2:91919/150‑1 (MQ=255)
    aggccAGGGAGAGAGCGTGGACTCGCCAGCAGAATCGAAAATTTTCCTCCCCATCATCCGCGCACCAGTCGCCGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGtt                                                                                                                                            <  2:3804676/147‑1 (MQ=255)
     gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTa                                                                                                                                           >  2:513986/1‑150 (MQ=255)
     gTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTa                                                                                                                                           >  2:4177824/1‑150 (MQ=255)
        cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                        <  2:614221/150‑1 (MQ=255)
        cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                        <  2:591191/150‑1 (MQ=255)
        cAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCg                                                                                                                                        >  2:2943382/1‑150 (MQ=255)
         aGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCgg                                                                                                                                       >  1:2819483/1‑150 (MQ=255)
              gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGAt                                                                                                                                  <  1:694140/150‑1 (MQ=255)
                 agCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATcgc                                                                                                                               >  1:1485946/1‑150 (MQ=255)
                     tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTa                                                                                                                           >  1:366927/1‑150 (MQ=255)
                        aCTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCg                                                                                                                        >  2:2889995/1‑150 (MQ=255)
                            gCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCAt                                                                                                                    >  2:2643916/1‑150 (MQ=255)
                             ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATg                                                                                                                   <  1:99190/150‑1 (MQ=255)
                              cagcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATgg                                                                                                                  >  1:2677527/1‑150 (MQ=255)
                               agcagAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGa                                                                                                                 <  2:1480126/150‑1 (MQ=255)
                                   gAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc                                                                                                             >  2:3155108/1‑150 (MQ=255)
                                     atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGc                                                                                                           >  1:1670898/1‑150 (MQ=255)
                                      tataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGcc                                                                                                          >  1:2718031/1‑150 (MQ=255)
                                       ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCa                                                                                                         <  1:160948/150‑1 (MQ=255)
                                        taAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAt                                                                                                        >  1:1055683/1‑150 (MQ=255)
                                         aaaaTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATg                                                                                                       >  2:3774300/1‑150 (MQ=255)
                                               ttCCTAAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTATCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTgg                                                                                                 >  1:2631923/1‑150 (MQ=255)
                                                tCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGa                                                                                                <  1:4043912/150‑1 (MQ=255)
                                                    cAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACTTAAATTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGATAGCGGTAGATCGCTTTATCTGAATGGAAAACGCCATGCCGTGGAAcct                                                                                            >  1:897736/1‑150 (MQ=255)
                                                     aaCATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctg                                                                                           <  1:2806071/150‑1 (MQ=255)
                                                        atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                        <  1:3814435/150‑1 (MQ=255)
                                                        atcatcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAAcctgcct                                                                                        <  1:3565247/150‑1 (MQ=255)
                                                          catcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACctgcctgc                                                                                      <  2:3951362/150‑1 (MQ=255)
                                                            tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCg                                                                                    <  1:1655400/150‑1 (MQ=255)
                                                             ccTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGa                                                                                   >  1:1915810/1‑150 (MQ=255)
                                                                cGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtct                                                                                >  1:642445/1‑150 (MQ=255)
                                                                 gCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtctc                                                                               >  1:454693/1‑150 (MQ=255)
                                                                 gCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAtctc                                                                               <  2:3494892/150‑1 (MQ=255)
                                                                  cACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCg                                                                              >  2:2770342/1‑150 (MQ=255)
                                                                   aCCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                                                             >  1:3084373/1‑150 (MQ=255)
                                                                   aCCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGc                                                                             >  1:3205987/1‑150 (MQ=255)
                                                                       gTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTgg                                                                         >  1:4380/1‑150 (MQ=255)
                                                                          gacgacGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                                                      <  2:2438038/150‑1 (MQ=255)
                                                                          gacgacGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATAGCTGAGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCAGGCCGTGGAACCTGCCTGCCGATCTCGCCTGGttt                                                                      >  2:1348293/1‑150 (MQ=255)
                                                                           acgacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa                                                                     <  1:2297708/150‑1 (MQ=255)
                                                                           acgacgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTa                                                                     <  2:3347915/150‑1 (MQ=255)
                                                                              tcgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc                                                                  <  1:4076098/149‑1 (MQ=255)
                                                                              acgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAAc                                                                  >  2:1728365/1‑150 (MQ=255)
                                                                               cgGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACg                                                                 <  1:2781226/150‑1 (MQ=255)
                                                                                 gTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCa                                                               >  1:2118433/1‑150 (MQ=255)
                                                                                   ttACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAac                                                             >  1:85134/1‑150 (MQ=255)
                                                                                        cTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCtt                                                        >  1:3425671/1‑150 (MQ=255)
                                                                                             cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata                                                   >  1:4137793/1‑150 (MQ=255)
                                                                                             cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata                                                   >  1:2564024/1‑150 (MQ=255)
                                                                                             cGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaata                                                   <  1:2026544/150‑1 (MQ=255)
                                                                                              gTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTtaaataa                                                  >  2:2685791/1‑150 (MQ=255)
                                                                                                ataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                <  1:300418/150‑1 (MQ=255)
                                                                                                ataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAc                                                >  2:235161/1‑150 (MQ=255)
                                                                                                ataGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAAGCCATGCCGTTGAACATGACTGCCGATCTCGCATGGTTTAAACGCAACACCTTAAATAAAc                                                >  2:1789303/1‑150 (MQ=255)
                                                                                                 taGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAcc                                               <  1:3019527/150‑1 (MQ=255)
                                                                                                  aGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                              <  2:932363/150‑1 (MQ=255)
                                                                                                  aGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                              <  1:1100769/150‑1 (MQ=255)
                                                                                                  aGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                              >  1:2410353/1‑150 (MQ=255)
                                                                                                   gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                             <  2:1371187/150‑1 (MQ=255)
                                                                                                   gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                             <  1:2277249/150‑1 (MQ=255)
                                                                                                   gTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCg                                             >  1:3132767/1‑150 (MQ=255)
                                                                                                     ggCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTg                                           <  1:2907752/150‑1 (MQ=255)
                                                                                                      gCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGa                                          >  2:3525640/1‑150 (MQ=255)
                                                                                                       cGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAt                                         <  1:1244185/150‑1 (MQ=255)
                                                                                                        gACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAtt                                        >  2:62868/1‑150 (MQ=255)
                                                                                                         aCAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTa                                       <  1:1870376/150‑1 (MQ=255)
                                                                                                           aaTTTTTTTTATCGGGAAATCCCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATg                                     >  2:2221492/1‑150 (MQ=255)
                                                                                                                tttttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccg                                >  1:1576945/1‑150 (MQ=255)
                                                                                                                  tttCTCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGgccgcc                              <  1:712340/150‑1 (MQ=255)
                                                                                                                  tttATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAGTATGGgccgcc                              >  2:2403868/1‑150 (MQ=255)
                                                                                                                    tATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                            >  2:3247874/1‑150 (MQ=255)
                                                                                                                    tATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                            <  2:3604685/150‑1 (MQ=255)
                                                                                                                    tATCGGGAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                            >  2:1024922/1‑150 (MQ=255)
                                                                                                                        gggAAATCTCACTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                        <  2:3732863/150‑1 (MQ=255)
                                                                                                                        gggAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCt                        <  1:1115993/150‑1 (MQ=255)
                                                                                                                         ggAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTg                       >  2:1133920/1‑150 (MQ=255)
                                                                                                                          gAAATCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTgg                      <  2:4015327/150‑1 (MQ=255)
                                                                                                                           aaaTCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                     >  1:3050408/1‑150 (MQ=255)
                                                                                                                           aaaTCTCAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTggg                     <  1:136918/150‑1 (MQ=255)
                                                                                                                            aaTCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGa                    >  1:411128/1‑150 (MQ=255)
                                                                                                                                tcAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca                >  2:673154/1‑150 (MQ=255)
                                                                                                                                 cAATGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaa               >  2:1225251/1‑150 (MQ=255)
                                                                                                                                  aaTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat              >  1:569413/1‑150 (MQ=255)
                                                                                                                                  aaTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat              >  1:2330455/1‑150 (MQ=255)
                                                                                                                                  aaTGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaat              >  1:362526/1‑150 (MQ=255)
                                                                                                                                    tGATCAGTCTGATTGCTGCGTTAGCGGTAGGTCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCg            <  1:553448/150‑1 (MQ=255)
                                                                                                                                    tGATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCg            <  2:765026/150‑1 (MQ=255)
                                                                                                                                     gATCAGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg           >  2:2698912/1‑150 (MQ=255)
                                                                                                                                         aGTCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtcgt       >  2:439223/1‑150 (MQ=255)
                                                                                                                                           tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc     <  2:2033974/150‑1 (MQ=255)
                                                                                                                                           tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc     <  2:3077985/150‑1 (MQ=255)
                                                                                                                                           tCTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTcc     <  1:383373/150‑1 (MQ=255)
                                                                                                                                            cTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg    <  2:1962628/150‑1 (MQ=255)
                                                                                                                                            cTGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCg    >  2:3305648/1‑150 (MQ=255)
                                                                                                                                             tGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt   <  1:2686936/150‑1 (MQ=255)
                                                                                                                                             tGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt   <  1:1478449/150‑1 (MQ=255)
                                                                                                                                             tGATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt   >  2:1355823/1‑150 (MQ=255)
                                                                                                                                              gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt  <  2:2576022/150‑1 (MQ=255)
                                                                                                                                              gATTGCTGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGtt  <  2:2652259/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                               
GGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGTT  >  CP009273/49692‑49983

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑