Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 549,397 G→T 100% intergenic (‑1/+2) lpxH ← / ← ppiB UDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273549,3970GT98.8% 860.3 / 1.7 246intergenic (‑1/+2)lpxH/ppiBUDP‑2,3‑diacylglucosamine pyrophosphohydrolase/peptidyl‑prolyl cis‑trans isomerase B (rotamase B)
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base T (109/134);  total (109/137)
Fisher's exact test for biased strand distribution p-value = 2.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 246 total aligned reads displayed.

GTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA  >  CP009273/549250‑549542
                                                                                                                                                   |                                                                                                                                                 
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                 <  1:1224566/150‑1 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                 >  2:4129448/1‑150 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                 <  1:1978941/150‑1 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCACTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                 <  2:2230693/150‑1 (MQ=255)
gtcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCACTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAt                                                                                                                                                 <  2:3842634/150‑1 (MQ=255)
 tcgCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTAtt                                                                                                                                                <  2:3083638/150‑1 (MQ=255)
   gCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTAc                                                                                                                                              >  2:573508/1‑150 (MQ=255)
         ccATGCTTCAAACAGAGCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTa                                                                                                                                        <  1:3202507/150‑1 (MQ=255)
          cATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTaa                                                                                                                                       <  2:2407512/150‑1 (MQ=255)
            tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACg                                                                                                                                     <  1:947144/150‑1 (MQ=255)
            tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACg                                                                                                                                     <  1:2871176/150‑1 (MQ=255)
            tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACg                                                                                                                                     >  2:270330/1‑150 (MQ=255)
            tGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACg                                                                                                                                     <  1:3700702/150‑1 (MQ=255)
               ttCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTc                                                                                                                                  <  2:3473818/150‑1 (MQ=255)
                tCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCa                                                                                                                                 >  1:2249927/1‑150 (MQ=255)
                tCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCa                                                                                                                                 >  2:2460489/1‑150 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                                <  1:2972892/150‑1 (MQ=255)
                 cAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCAc                                                                                                                                <  1:2292109/150‑1 (MQ=255)
                  aaaCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACg                                                                                                                               >  1:1318112/1‑150 (MQ=255)
                   aaCAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGc                                                                                                                              <  2:854818/150‑1 (MQ=255)
                     cAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCtt                                                                                                                            <  1:3908226/150‑1 (MQ=255)
                      aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCttt                                                                                                                           <  1:2038485/150‑1 (MQ=255)
                      aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCttt                                                                                                                           >  1:3816542/1‑150 (MQ=255)
                      aGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCttt                                                                                                                           >  1:2753788/1‑150 (MQ=255)
                         tCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCaa                                                                                                                        >  1:2703298/1‑150 (MQ=255)
                          cGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAAt                                                                                                                       >  1:3625178/1‑150 (MQ=255)
                           gCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAACGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATg                                                                                                                      >  2:2790888/1‑150 (MQ=255)
                           gCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATg                                                                                                                      >  1:351851/1‑150 (MQ=255)
                              aaGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATa                                                                                                                   <  2:2811227/150‑1 (MQ=255)
                                gAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAAc                                                                                                                 <  2:195711/150‑1 (MQ=255)
                                 aaTATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACg                                                                                                                >  2:927572/1‑150 (MQ=255)
                                  atatACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGt                                                                                                               <  2:1917462/150‑1 (MQ=255)
                                      aCAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGtcttc                                                                                                           <  1:4036721/150‑1 (MQ=255)
                                        aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTCACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                         >  1:2612381/1‑150 (MQ=255)
                                        aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                         <  1:996533/150‑1 (MQ=255)
                                        aGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTcttctt                                                                                                         >  2:1441977/1‑150 (MQ=255)
                                          cgcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCtttt                                                                                                       <  1:893306/150‑1 (MQ=255)
                                           gcgTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTg                                                                                                      <  2:1536992/150‑1 (MQ=255)
                                           gcgTCGGCCTTGCGGGATTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTg                                                                                                      <  2:1685125/150‑1 (MQ=255)
                                             gTCGGCCTTGTGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGc                                                                                                    <  2:3998931/150‑1 (MQ=255)
                                              tCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCa                                                                                                   >  1:937558/1‑150 (MQ=255)
                                              tCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCa                                                                                                   <  1:3799156/150‑1 (MQ=255)
                                              tCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCa                                                                                                   >  1:2522804/1‑150 (MQ=255)
                                               cGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCAc                                                                                                  <  2:1755664/150‑1 (MQ=255)
                                                ggCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGACGATCTGCAATAACGAGTGTCGCCACTCTTACTCGCTAACGGTCACGCTTTCAATGATCACGTCTTCTTTTGGCACg                                                                                                 <  2:318132/150‑1 (MQ=255)
                                                        gggCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTg                                                                                         >  1:2615750/1‑150 (MQ=255)
                                                          gCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCa                                                                                       >  1:3251431/1‑150 (MQ=255)
                                                           cTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAt                                                                                      >  1:3428964/1‑150 (MQ=255)
                                                           cTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCAt                                                                                      <  1:1828691/150‑1 (MQ=255)
                                                             tCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAc                                                                                    >  1:1824014/1‑150 (MQ=255)
                                                              cccccGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATAcc                                                                                   <  1:3552663/150‑1 (MQ=255)
                                                                  cGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTa                                                                               <  1:1304601/150‑1 (MQ=255)
                                                                      aaaaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGAc                                                                           >  2:1883539/1‑150 (MQ=255)
                                                                        aaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTCCGACCg                                                                         >  1:218120/1‑150 (MQ=255)
                                                                        aaaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCg                                                                         <  1:1587930/150‑1 (MQ=255)
                                                                         aaaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCgg                                                                        >  2:4045003/1‑150 (MQ=255)
                                                                          aaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGt                                                                       >  1:1103104/1‑150 (MQ=255)
                                                                          aaCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGt                                                                       >  1:1459078/1‑150 (MQ=255)
                                                                           aCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGTGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGtt                                                                      <  1:1663604/150‑1 (MQ=255)
                                                                           aCGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGtt                                                                      <  2:339418/150‑1 (MQ=255)
                                                                            cGCAGACAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCGACGACCGGTTg                                                                     <  1:829865/150‑1 (MQ=255)
                                                                             gCAGAACACCGGCGGTGATCGCCGGTTCTACCACGCAGAGATGACGATCTGCAATACAGAGTGTCGCCACTCTTACTCGCTACCGGTCACGCTGTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGc                                                                    <  2:1175230/150‑1 (MQ=255)
                                                                              cAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCa                                                                   <  2:1092352/150‑1 (MQ=255)
                                                                                 aaaaCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAaca                                                                >  1:4172658/1‑150 (MQ=255)
                                                                                   aaCCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACAcc                                                              >  1:484412/1‑150 (MQ=255)
                                                                                       ggcggTGATCGCCGGTACTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTg                                                          <  2:2200121/150‑1 (MQ=255)
                                                                                        gcggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGa                                                         <  1:1045574/150‑1 (MQ=255)
                                                                                         cggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAt                                                        <  2:199962/150‑1 (MQ=255)
                                                                                          ggTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAtt                                                       >  1:2935901/1‑150 (MQ=255)
                                                                                           gTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAttt                                                      <  2:2497138/150‑1 (MQ=255)
                                                                                           gTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGAttt                                                      >  2:3204086/1‑150 (MQ=255)
                                                                                             gATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTg                                                    >  1:2921172/1‑150 (MQ=255)
                                                                                              aTCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGt                                                   >  2:394627/1‑150 (MQ=255)
                                                                                              aTCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGt                                                   >  1:1418741/1‑150 (MQ=255)
                                                                                               tCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTc                                                  >  1:2357567/1‑150 (MQ=255)
                                                                                                cGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCt                                                 <  1:1252277/150‑1 (MQ=255)
                                                                                                 gCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTa                                                >  1:764319/1‑150 (MQ=255)
                                                                                                  ccGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAc                                               <  2:1318112/150‑1 (MQ=255)
                                                                                                  ccGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTAc                                               <  1:2968127/150‑1 (MQ=255)
                                                                                                    ggTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCa                                             >  2:1221394/1‑150 (MQ=255)
                                                                                                       tcttcCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGt                                          >  1:2911100/1‑150 (MQ=255)
                                                                                                            cACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCGTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTAGCAACACCTTTGATGTTGTCTACCACGTCCATg                                     >  1:1734489/1‑150 (MQ=255)
                                                                                                             aCGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGc                                    >  2:2727331/1‑150 (MQ=255)
                                                                                                             aCGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGc                                    >  1:516995/1‑150 (MQ=255)
                                                                                                              cGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGcc                                   <  2:450538/150‑1 (MQ=255)
                                                                                                               gCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCg                                  <  1:1747890/150‑1 (MQ=255)
                                                                                                                cAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGt                                 <  1:1531438/150‑1 (MQ=255)
                                                                                                                       gAAGATCTGCAATAAAGAGTGTCGCCACTATTACACGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCAAGTCCTGGTGCATACAGCTACGACCGGATGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGACAAacac                          >  1:3036461/1‑150 (MQ=255)
                                                                                                                        aaGATCTGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACt                         >  2:1286111/1‑150 (MQ=255)
                                                                                                                             cTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGc                    <  1:3366289/150‑1 (MQ=255)
                                                                                                                              tGCAATAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCa                   >  1:233395/1‑150 (MQ=255)
                                                                                                                                 aaTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAac                >  2:3517219/1‑150 (MQ=255)
                                                                                                                                  aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca               >  1:2050672/1‑150 (MQ=255)
                                                                                                                                  aTAAAGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAaca               >  1:310775/1‑150 (MQ=255)
                                                                                                                                    aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg             >  2:2950626/1‑150 (MQ=255)
                                                                                                                                    aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg             <  2:538869/150‑1 (MQ=255)
                                                                                                                                    aaaGAGTGTCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACg             >  1:2762100/1‑150 (MQ=255)
                                                                                                                                         gtgtCGCCACTATTACTCGCTAACTGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTa        <  2:3601275/150‑1 (MQ=255)
                                                                                                                                           gtCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGc      <  2:3642190/150‑1 (MQ=255)
                                                                                                                                           gtCGCCACTATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGc      >  1:1212868/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GTCGCCAATCCATGCTTCAAACAGATCGCCAAGAATATACAGCGCGTCGGCCTTGCGGGCTTCCCCCGCTAAAAAACGCAGAAAACCGGCGGTGATCGCCGGTTCTTCCACGCAGAGATGAAGATCTGCAATAAAGAGTGTCGCCACGATTACTCGCTAACGGTCACGCTTTCAATGATAACGTCTTCTTTTGGCACGTCCTGGTGCATACCGCTACGACCGGTTGCAACACCTTTGATTTTGTCTACCACGTCCATGCCGTCAACCACTTCAGCAAACACGCAGTAGCCCCA  >  CP009273/549250‑549542

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑