BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	734,498	C→A	6.7%	I14I (ATC→ATA)	ybgA →	DUF1722 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	734,498	0	C	A	6.7%	872.1 / 26.3	225	I14I (ATC→ATA)	ybgA	DUF1722 family protein
Reads supporting (aligned to +/- strand): ref base C (113/97); new base A (9/6); total (122/103)
Fisher's exact test for biased strand distribution p-value = 7.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 229 total aligned reads displayed.

TGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCA  >  CP009273/734350‑734647

tGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCt                                                                                                                                                      <  2:3559317/150‑1 (MQ=255)

  cGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCttt                                                                                                                                                    <  2:2264239/150‑1 (MQ=255)

        gCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCtt                                                                                                                                              <  2:2172467/150‑1 (MQ=255)

         cAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTc                                                                                                                                             <  1:3549433/150‑1 (MQ=255)

           ggCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCAt                                                                                                                                           <  2:3884004/150‑1 (MQ=255)

            gCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATg                                                                                                                                          >  1:2126320/1‑150 (MQ=255)

             cTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGa                                                                                                                                         >  2:709818/1‑150 (MQ=255)

              tttCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAg                                                                                                                                        >  2:2885947/1‑150 (MQ=255)

              tttCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAg                                                                                                                                        <  1:1019324/150‑1 (MQ=255)

                tCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGtt                                                                                                                                      <  2:448881/150‑1 (MQ=255)

                   tAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACa                                                                                                                                   >  1:3818020/1‑150 (MQ=255)

                   tAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACa                                                                                                                                   <  1:4074988/150‑1 (MQ=255)

                    aaaTTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACAt                                                                                                                                  <  2:3392018/150‑1 (MQ=255)

                          gTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTg                                                                                                                            >  1:2695506/1‑150 (MQ=255)

                             cTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGaaa                                                                                                                         >  1:3955267/1‑150 (MQ=255)

                             cTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGaaa                                                                                                                         >  2:2683325/1‑150 (MQ=255)

                               gCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGa                                                                                                                       <  1:2680126/150‑1 (MQ=255)

                                cTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGaa                                                                                                                      >  2:3140329/1‑150 (MQ=255)

                                  acaacaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACa                                                                                                                    >  1:1604716/1‑150 (MQ=255)

                                    aacaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATg                                                                                                                  >  2:2457887/1‑150 (MQ=255)

                                     acaGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATgg                                                                                                                 <  1:310474/150‑1 (MQ=255)

                                       aGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCt                                                                                                               <  2:4160624/150‑1 (MQ=255)

                                            tAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAAcg                                                                                                          >  1:1211029/1‑150 (MQ=255)

                                              aCTTCACAAATATCATTTCTCAACGTCTACACATACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAAcgcg                                                                                                        >  1:98100/1‑150 (MQ=255)

                                                  cacaAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCggg                                                                                                    >  2:1460907/1‑150 (MQ=255)

                                                  cacaAATATAATTTCTCAAAGTCTACACTAACTACTGTAAACAGCTAAGTAGCAGTAATGAATCTACAAAGATTAGATGACAGCACCCTAATCCGTATCTATTCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTAAACGCGCtgg                                                                                                    >  2:2477861/1‑150 (MQ=255)

                                                    caAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgc                                                                                                  <  1:1762794/150‑1 (MQ=255)

                                                    caAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgc                                                                                                  <  2:2036089/150‑1 (MQ=255)

                                                     aaaTATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgcg                                                                                                 >  1:2337453/1‑150 (MQ=255)

                                                     aaaTATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGgcg                                                                                                 <  1:582946/150‑1 (MQ=255)

                                                       atatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCt                                                                                               <  1:3042947/150‑1 (MQ=255)

                                                        tatCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATAGACTGAAAGAACATGGCTTAACGCGCGGGGCGCtt                                                                                              >  2:2626358/1‑150 (MQ=255)

                                                          tCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtct                                                                                            <  2:3564945/150‑1 (MQ=255)

                                                           cATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtctc                                                                                           <  2:549258/150‑1 (MQ=255)

                                                           cATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtctc                                                                                           <  1:1127005/150‑1 (MQ=255)

                                                           cATTTATCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTtctc                                                                                           <  2:49638/150‑1 (MQ=255)

                                                            aTTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCg                                                                                          >  1:1996214/1‑150 (MQ=255)

                                                              ttCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAt                                                                                        >  1:2228701/1‑150 (MQ=255)

                                                               tctcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGAtt                                                                                       >  2:507198/1‑150 (MQ=255)

                                                                ctcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTa                                                                                      >  1:696864/1‑150 (MQ=255)

                                                                 tcAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTAt                                                                                     >  1:1283731/1‑150 (MQ=255)

                                                                   aaCGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATca                                                                                   <  2:1145515/150‑1 (MQ=255)

                                                                     cGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATcaca                                                                                 >  1:4175158/1‑150 (MQ=255)

                                                                         tACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCg                                                                             >  1:1165910/1‑150 (MQ=255)

                                                                           cacTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCt                                                                           <  2:940007/150‑1 (MQ=255)

                                                                           cacTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCt                                                                           <  2:3164010/150‑1 (MQ=255)

                                                                                aCTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTAaaaa                                                                      >  2:182391/1‑150 (MQ=255)

                                                                                aCTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATaaa                                                                      >  2:1063064/1‑150 (MQ=255)

                                                                                aCTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATaaa                                                                      >  2:4089578/1‑150 (MQ=255)

                                                                                  tCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACt                                                                    >  1:472411/1‑150 (MQ=255)

                                                                                  tCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACt                                                                    <  1:3896431/150‑1 (MQ=255)

                                                                                   ccTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTc                                                                   >  2:3296193/1‑150 (MQ=255)

                                                                                     tGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGt                                                                 >  1:1835785/1‑150 (MQ=255)

                                                                                     tGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGt                                                                 <  1:2576397/150‑1 (MQ=255)

                                                                                       tAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCt                                                               <  1:2745080/150‑1 (MQ=255)

                                                                                       tAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACAACGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCt                                                               >  1:3487782/1‑150 (MQ=255)

                                                                                         aaCCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCttt                                                             <  1:227259/150‑1 (MQ=255)

                                                                                         aaCCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCttt                                                             <  1:583645/150‑1 (MQ=255)

                                                                                         aaCCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCttt                                                             <  1:375398/150‑1 (MQ=255)

                                                                                           ccGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCt                                                           <  2:89796/150‑1 (MQ=255)

                                                                                             gCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTgg                                                         <  1:213972/150‑1 (MQ=255)

                                                                                               tCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGgcg                                                       <  2:1587824/150‑1 (MQ=255)

                                                                                                 aGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCa                                                     >  1:2876949/1‑150 (MQ=255)

                                                                                                 aGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCa                                                     <  2:3419590/150‑1 (MQ=255)

                                                                                                 aGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCGATAAACTCGTCTTAATGGCGCa                                                     >  1:980681/1‑150 (MQ=255)

                                                                                                   gAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCAtt                                                   >  1:4018400/1‑150 (MQ=255)

                                                                                                    aGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATtc                                                  <  1:710042/150‑1 (MQ=255)

                                                                                                     gCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATtct                                                 <  2:845093/150‑1 (MQ=255)

                                                                                                     gCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATtct                                                 >  1:923484/1‑150 (MQ=255)

                                                                                                        gTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAg                                              >  1:3304035/1‑150 (MQ=255)

                                                                                                          aaTGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGcc                                            <  1:785165/150‑1 (MQ=255)

                                                                                                          aaTGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGcc                                            <  1:1368250/150‑1 (MQ=255)

                                                                                                             gAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGa                                         >  1:2391657/1‑150 (MQ=255)

                                                                                                                tCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTa                                      >  2:149104/1‑150 (MQ=255)

                                                                                                                 cTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTAc                                     <  1:3064758/150‑1 (MQ=255)

                                                                                                                 cTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTAc                                     >  1:3413317/1‑150 (MQ=255)

                                                                                                                     aaCGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCa                                 >  2:2757999/1‑150 (MQ=255)

                                                                                                                       cGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCaaa                               <  2:1730437/150‑1 (MQ=255)

                                                                                                                         aTTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACACGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACt                             <  1:3840493/150‑1 (MQ=255)

                                                                                                                          tttGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACtt                            <  1:3312022/150‑1 (MQ=255)

                                                                                                                          tttGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACtt                            >  1:3248465/1‑150 (MQ=255)

                                                                                                                            tgatgaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTgg                          >  2:3467011/1‑150 (MQ=255)

                                                                                                                             gatgaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGc                         <  2:544094/150‑1 (MQ=255)

                                                                                                                               tgaCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGccc                       >  1:2691979/1‑150 (MQ=255)

                                                                                                                                 aCAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGt                     >  1:2315612/1‑150 (MQ=255)

                                                                                                                                  cAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGtt                    >  1:3180455/1‑150 (MQ=255)

                                                                                                                                   aGCACCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTc                   <  2:4129166/150‑1 (MQ=255)

                                                                                                                                      aCCCTAATCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTg                >  2:1159966/1‑150 (MQ=255)

                                                                                                                                         cTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCt             <  2:405145/150‑1 (MQ=255)

                                                                                                                                         cTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCt             <  2:3997349/150‑1 (MQ=255)

                                                                                                                                          tAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTg            <  1:89367/150‑1 (MQ=255)

                                                                                                                                            aTCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGat          >  1:1946097/1‑150 (MQ=255)

                                                                                                                                            aTCCGTATATTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGat          >  1:4170575/1‑150 (MQ=255)

                                                                                                                                                 tATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCa     <  1:520221/150‑1 (MQ=255)

                                                                                                                                                 tATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCa     <  1:1790805/150‑1 (MQ=255)

                                                                                                                                                  aTCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCAc    <  2:964275/150‑1 (MQ=255)

                                                                                                                                                  aTCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCAc    <  2:964854/150‑1 (MQ=255)

                                                                                                                                                   tCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCAcc   >  1:664440/1‑150 (MQ=255)

                                                                                                                                                    cTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCa  <  2:4029903/150‑1 (MQ=255)

                                                                                                                                                    cTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCa  <  2:1558848/150‑1 (MQ=255)

TGCGGGGAGCAGGCTTTCGTAAATTTGTCCTGCTACAACAGGATTAACTTCACAAATATCATTTCTCAACGTCTACACTTACTCCTGTAAACCGCTCAGGAGCAGTAATGAATCTACAACGATTTGATGACAGCACCCTAATCCGTATCTTTGCCCTTCATGAGTTACATCGACTGAAAGAACATGGCTTAACGCGCGGGGCGCTTCTCGATTATCACAGCCGCTATAAACTCGTCTTTCTGGCGCATTCTCAGCCGGAGTACCGCAAACTTGGCCCGTTCGTGGCTGATATTCACCA  >  CP009273/734350‑734647

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`