Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 81,562 C→A 100% I236I (ATC→ATA)  leuO → global transcription factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927381,5620CA96.3% 782.6 / 19.6 241I236I (ATC→ATA) leuOglobal transcription factor
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (141/90);  total (146/95)
Fisher's exact test for biased strand distribution p-value = 7.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 251 total aligned reads displayed.

GCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAG  >  CP009273/81415‑81708
                                                                                                                                                   |                                                                                                                                                  
 cTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATagcg                                                                                                                                                 <  2:2551092/150‑1 (MQ=255)
  tGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGt                                                                                                                                                >  1:2527385/1‑150 (MQ=255)
      aGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACCTGATGTTTATCACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCa                                                                                                                                            <  1:397984/150‑1 (MQ=255)
           gCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCa                                                                                                                                       <  2:3584487/150‑1 (MQ=255)
              aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATgg                                                                                                                                    <  2:2045927/150‑1 (MQ=255)
              aaaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATgg                                                                                                                                    <  1:2091591/150‑1 (MQ=255)
               aaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGc                                                                                                                                   >  1:331947/1‑150 (MQ=255)
               aaaTCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGc                                                                                                                                   <  2:2117886/150‑1 (MQ=255)
                   catcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatg                                                                                                                               <  2:2612720/150‑1 (MQ=255)
                   catcCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatg                                                                                                                               >  1:2056615/1‑150 (MQ=255)
                      ccAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAatgatg                                                                                                                            >  1:3591732/1‑150 (MQ=255)
                        aacaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAg                                                                                                                          >  2:577470/1‑150 (MQ=255)
                         acaaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGc                                                                                                                         <  1:3366862/150‑1 (MQ=255)
                          caaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCg                                                                                                                        <  1:630170/150‑1 (MQ=255)
                          caaTTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCg                                                                                                                        <  1:1805087/150‑1 (MQ=255)
                            aTTAAGGGCCCGTTACTGAAACAGGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTa                                                                                                                      <  2:948853/150‑1 (MQ=255)
                              tAAGGGCCCGTTACTGAAACCTGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                    <  1:3209785/150‑1 (MQ=255)
                              tAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACt                                                                                                                    >  1:823368/1‑150 (MQ=255)
                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                                                                                                                  >  2:3258403/1‑150 (MQ=255)
                                aGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTa                                                                                                                  <  2:2910947/150‑1 (MQ=255)
                                  ggCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGc                                                                                                                <  1:2734077/150‑1 (MQ=255)
                                      cGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtgg                                                                                                            >  2:3885413/1‑150 (MQ=255)
                                        ttACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCgtggtg                                                                                                          >  2:70203/1‑150 (MQ=255)
                                         tACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGgtgt                                                                                                         >  2:1317615/1‑150 (MQ=255)
                                          aCTGCAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTc                                                                                                        <  2:1413631/150‑1 (MQ=255)
                                           cTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCg                                                                                                       >  1:434824/1‑150 (MQ=255)
                                            tGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                      >  1:1779972/1‑150 (MQ=255)
                                            tGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGc                                                                                                      >  1:1551137/1‑150 (MQ=255)
                                              aaaCATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCaa                                                                                                    >  2:2670638/1‑150 (MQ=255)
                                                  atgatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGc                                                                                                >  1:2046578/1‑150 (MQ=255)
                                                    gatgTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCAt                                                                                              <  2:1524240/150‑1 (MQ=255)
                                                          tataACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTc                                                                                        <  2:2465064/150‑1 (MQ=255)
                                                             aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCt                                                                                     <  2:1390351/150‑1 (MQ=255)
                                                             aaCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCt                                                                                     >  1:883296/1‑150 (MQ=255)
                                                              aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTa                                                                                    >  1:559642/1‑150 (MQ=255)
                                                              aCGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTa                                                                                    >  2:3411517/1‑150 (MQ=255)
                                                                gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAtt                                                                                  <  2:1911941/150‑1 (MQ=255)
                                                                gAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTAtt                                                                                  >  1:1689196/1‑150 (MQ=255)
                                                                  acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgc                                                                                >  1:2213706/1‑150 (MQ=255)
                                                                  acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgc                                                                                >  2:1196609/1‑150 (MQ=255)
                                                                  acaacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgc                                                                                <  1:2068723/150‑1 (MQ=255)
                                                                   caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcg                                                                               >  2:149567/1‑150 (MQ=255)
                                                                   caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcg                                                                               <  1:1370813/150‑1 (MQ=255)
                                                                   caacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcg                                                                               <  1:2152349/150‑1 (MQ=255)
                                                                    aacaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTgcgc                                                                              >  2:1132319/1‑150 (MQ=255)
                                                                     acaTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGcgcc                                                                             >  1:103916/1‑150 (MQ=255)
                                                                      caTGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGcgccg                                                                            <  1:3757617/150‑1 (MQ=255)
                                                                           ggcggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtg                                                                       >  1:1895786/1‑150 (MQ=255)
                                                                              ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                    >  1:771442/1‑150 (MQ=255)
                                                                              ggTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTtggct                                                                    <  1:2551655/150‑1 (MQ=255)
                                                                                     cTCGATCGGTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGaa                                                             <  2:3688613/150‑1 (MQ=255)
                                                                                      tCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAag                                                            >  2:2715575/1‑150 (MQ=255)
                                                                                           cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTc                                                       <  2:4114717/150‑1 (MQ=255)
                                                                                           cGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTc                                                       >  2:1199579/1‑150 (MQ=255)
                                                                                            gTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCg                                                      <  2:3556609/150‑1 (MQ=255)
                                                                                              ttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCt                                                    <  2:1719174/150‑1 (MQ=255)
                                                                                              ttCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCt                                                    >  2:2957908/1‑150 (MQ=255)
                                                                                                cgcgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGa                                                  <  2:2647932/150‑1 (MQ=255)
                                                                                                  cgTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAAt                                                >  1:2271223/1‑150 (MQ=255)
                                                                                                   gTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATc                                               >  2:309733/1‑150 (MQ=255)
                                                                                                      aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCtt                                            >  1:546906/1‑150 (MQ=255)
                                                                                                      aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCtt                                            <  1:2677952/150‑1 (MQ=255)
                                                                                                      aTTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCtt                                            >  2:3299774/1‑150 (MQ=255)
                                                                                                        ttAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAg                                          >  2:3351722/1‑150 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       <  2:162173/150‑1 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       >  2:770026/1‑150 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       >  1:2462158/1‑150 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       <  1:421702/150‑1 (MQ=255)
                                                                                                           gTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATGTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAt                                       >  1:3656034/1‑150 (MQ=255)
                                                                                                            tCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAAtt                                      >  2:2237213/1‑150 (MQ=255)
                                                                                                               aCCTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACa                                   >  1:161986/1‑150 (MQ=255)
                                                                                                                 cTTGGTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAgg                                 <  1:2503112/150‑1 (MQ=255)
                                                                                                                     gTATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTAtt                             >  1:1322377/1‑150 (MQ=255)
                                                                                                                      tATGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTa                            >  2:43088/1‑150 (MQ=255)
                                                                                                                       aTGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAc                           <  1:1067302/150‑1 (MQ=255)
                                                                                                                       aTGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAc                           >  2:3526661/1‑150 (MQ=255)
                                                                                                                        tGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAcc                          <  1:1914252/150‑1 (MQ=255)
                                                                                                                        tGACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTAcc                          <  1:3045954/150‑1 (MQ=255)
                                                                                                                         gACACGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCg                         >  2:1247922/1‑150 (MQ=255)
                                                                                                                           cacGGTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCt                       <  2:1590054/150‑1 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    <  1:2885685/150‑1 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    <  1:3123133/150‑1 (MQ=255)
                                                                                                                              ggTAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGcc                    >  1:1957930/1‑150 (MQ=255)
                                                                                                                                tAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                  >  2:2627933/1‑150 (MQ=255)
                                                                                                                                tAGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGGTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGt                  >  1:1951015/1‑150 (MQ=255)
                                                                                                                                 aGATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGtt                 <  2:2585274/150‑1 (MQ=255)
                                                                                                                                  gATAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTa                >  1:876110/1‑150 (MQ=255)
                                                                                                                                   aTAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaa               >  2:2303891/1‑150 (MQ=255)
                                                                                                                                    tAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaa              <  1:206859/150‑1 (MQ=255)
                                                                                                                                    tAAGCAAGCCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaa              >  1:4014068/1‑150 (MQ=255)
                                                                                                                                      agcaagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaac            >  1:3486754/1‑150 (MQ=255)
                                                                                                                                      agcaagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaac            <  1:2904556/150‑1 (MQ=255)
                                                                                                                                         aagcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaa         >  1:3665360/1‑150 (MQ=255)
                                                                                                                                          agcCAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTaaaacaaaa        <  1:644183/150‑1 (MQ=255)
                                                                                                                                            ccAGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAaaacaaaaca      <  1:1494544/150‑1 (MQ=255)
                                                                                                                                              aGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc    >  1:2995677/1‑150 (MQ=255)
                                                                                                                                              aGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc    >  1:905580/1‑150 (MQ=255)
                                                                                                                                              aGTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagc    >  2:3718159/1‑150 (MQ=255)
                                                                                                                                               gTATAGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagca   >  2:1062072/1‑150 (MQ=255)
                                                                                                                                                tataGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAAcagcag  >  1:2769976/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GCTGGTAGCCAGCAAAAATCATCCAACAATTAAGGGCCCGTTACTGAAACATGATGTTTATAACGAACAACATGCGGCGGTTTCGCTCGATCGTTTCGCGTCATTTAGTCAACCTTGGTATGACACGGTAGATAAGCAAGCCAGTATCGCGTATCAGGGCATGGCAATGATGAGCGTACTTAGCGTGGTGTCGCAAACGCATTTGGTCGCTATTGCGCCGCGTTGGCTGGCTGAAGAGTTCGCTGAATCCTTAGAATTACAGGTATTACCGCTGCCGTTAAAACAAAACAGCAG  >  CP009273/81415‑81708

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑