Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 759,000 C→A 100% F177L (TTC→TTA)  sucC → succinyl‑CoA synthetase, beta subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273759,0000CA98.5% 933.1 / 3.9 269F177L (TTC→TTA) sucCsuccinyl‑CoA synthetase, beta subunit
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (146/119);  total (147/122)
Fisher's exact test for biased strand distribution p-value = 3.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 269 total aligned reads displayed.

AGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGCCA  >  CP009273/758853‑759146
                                                                                                                                                   |                                                                                                                                                  
aGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAc                                                                                                                                                  >  1:1348494/1‑150 (MQ=255)
 ggcggcGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAAcc                                                                                                                                                 <  1:1922176/150‑1 (MQ=255)
   cggcgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCaa                                                                                                                                               >  2:5717/1‑150 (MQ=255)
     gcgTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCaaaa                                                                                                                                             <  1:3279538/150‑1 (MQ=255)
       gTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATc                                                                                                                                           >  1:4082652/1‑150 (MQ=255)
       gTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATc                                                                                                                                           >  2:315057/1‑150 (MQ=255)
       gTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATc                                                                                                                                           <  1:1600639/150‑1 (MQ=255)
        tGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCt                                                                                                                                          <  2:2043647/150‑1 (MQ=255)
           aaaTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCa                                                                                                                                       >  2:197291/1‑150 (MQ=255)
           aaaTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCa                                                                                                                                       <  2:519755/150‑1 (MQ=255)
            aaTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGGTGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCAt                                                                                                                                      >  1:1138049/1‑150 (MQ=255)
             aTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATg                                                                                                                                     >  1:3332056/1‑150 (MQ=255)
             aTCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATg                                                                                                                                     >  2:3310623/1‑150 (MQ=255)
                gAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGc                                                                                                                                  <  1:1027026/150‑1 (MQ=255)
                gAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGc                                                                                                                                  <  1:239184/150‑1 (MQ=255)
                   aaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                               <  2:3904144/150‑1 (MQ=255)
                   aaaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTg                                                                                                                               >  2:448389/1‑150 (MQ=255)
                    aaGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTgg                                                                                                                              <  2:2786727/150‑1 (MQ=255)
                       tGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCGGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGa                                                                                                                           <  2:895900/150‑1 (MQ=255)
                       tGGCGGAAGAAACTACTCACCTGATCCATAAATTTGCGCTTGATCCGCTGACTGGCCCGATGACGTATCAGGGACGCGAGCTGGCGTTCAAACTGTGTATGGATGGTAATCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGa                                                                                                                           <  1:294573/150‑1 (MQ=255)
                        ggCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAATCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGAc                                                                                                                          >  2:235859/1‑150 (MQ=255)
                         gCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGAcc                                                                                                                         <  1:62054/150‑1 (MQ=255)
                              agaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtttt                                                                                                                    >  2:3366045/1‑150 (MQ=255)
                              agaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCAtttt                                                                                                                    <  1:3708692/150‑1 (MQ=255)
                               gaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTc                                                                                                                   >  2:2480016/1‑150 (MQ=255)
                               gaaACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTc                                                                                                                   <  2:4050825/150‑1 (MQ=255)
                                aaaCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTcc                                                                                                                  >  2:1734004/1‑150 (MQ=255)
                                  aCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTg                                                                                                                <  1:2670724/150‑1 (MQ=255)
                                  aCTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTg                                                                                                                >  1:2174146/1‑150 (MQ=255)
                                   cTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTgg                                                                                                               >  2:3392478/1‑150 (MQ=255)
                                   cTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTgg                                                                                                               <  2:3712179/150‑1 (MQ=255)
                                     ccGCACCTGATCCATAAAGTTGCGCTAGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAg                                                                                                             <  1:2371084/150‑1 (MQ=255)
                                       gCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAgcg                                                                                                           <  1:3468831/150‑1 (MQ=255)
                                        cACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGCATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAgcgc                                                                                                          >  1:2114045/1‑150 (MQ=255)
                                          ccTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGa                                                                                                        >  2:1387409/1‑150 (MQ=255)
                                            tGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGAcc                                                                                                      >  2:3222981/1‑150 (MQ=255)
                                             gATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCt                                                                                                     >  1:94486/1‑150 (MQ=255)
                                             gATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGCATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCt                                                                                                     <  2:3302043/150‑1 (MQ=255)
                                              aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTg                                                                                                    >  2:1194407/1‑150 (MQ=255)
                                              aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTg                                                                                                    <  2:1885060/150‑1 (MQ=255)
                                              aTCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTg                                                                                                    <  1:3032047/150‑1 (MQ=255)
                                               tCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTgg                                                                                                   >  1:3069143/1‑150 (MQ=255)
                                                  aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGt                                                                                                <  2:1390139/150‑1 (MQ=255)
                                                  aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGt                                                                                                <  2:1626241/150‑1 (MQ=255)
                                                  aTAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGt                                                                                                <  1:896892/150‑1 (MQ=255)
                                                    aaaGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTg                                                                                              >  1:390620/1‑150 (MQ=255)
                                                     aaGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGGCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGa                                                                                             >  2:2385531/1‑150 (MQ=255)
                                                      aGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGAt                                                                                            >  1:2322506/1‑150 (MQ=255)
                                                       gTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATc                                                                                           <  1:2275280/150‑1 (MQ=255)
                                                        ttGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCg                                                                                          >  1:3167657/1‑150 (MQ=255)
                                                         tGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGa                                                                                         >  2:663282/1‑150 (MQ=255)
                                                         tGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGa                                                                                         <  2:3847157/150‑1 (MQ=255)
                                                           cgcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGaaa                                                                                       >  2:2717857/1‑150 (MQ=255)
                                                           cgcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGaaa                                                                                       <  2:3993168/150‑1 (MQ=255)
                                                            gcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAAt                                                                                      >  1:1807617/1‑150 (MQ=255)
                                                            gcTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAAt                                                                                      >  1:2316103/1‑150 (MQ=255)
                                                                 aTCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAcc                                                                                 >  2:2952609/1‑150 (MQ=255)
                                                                 aTCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCATGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAAcc                                                                                 >  1:2745974/1‑150 (MQ=255)
                                                                    cGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGc                                                                              >  2:2253138/1‑150 (MQ=255)
                                                                     gCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCt                                                                             <  1:3542500/150‑1 (MQ=255)
                                                                       tGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTgg                                                                           >  2:2585478/1‑150 (MQ=255)
                                                                         aCTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGtc                                                                         >  1:67858/1‑150 (MQ=255)
                                                                            ggCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGtcatc                                                                      >  1:918609/1‑150 (MQ=255)
                                                                                   tGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAAc                                                               >  2:808980/1‑150 (MQ=255)
                                                                                        tATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGc                                                          <  1:1104676/150‑1 (MQ=255)
                                                                                           cAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGAt                                                       <  2:2893518/150‑1 (MQ=255)
                                                                                            aGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATc                                                      >  2:361660/1‑150 (MQ=255)
                                                                                               gACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGa                                                   <  2:1107953/150‑1 (MQ=255)
                                                                                                 cgcgAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTGTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGAtt                                                 <  1:2531991/150‑1 (MQ=255)
                                                                                                    gAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGc                                              >  2:2356600/1‑150 (MQ=255)
                                                                                                           cGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACg                                       <  2:870320/150‑1 (MQ=255)
                                                                                                                 aaCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAAc                                 <  1:3507214/150‑1 (MQ=255)
                                                                                                                  aCTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACt                                <  2:4136013/150‑1 (MQ=255)
                                                                                                                    tGGGTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTgg                              >  1:4113360/1‑150 (MQ=255)
                                                                                                                     gggTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGAGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTggg                             >  1:496133/1‑150 (MQ=255)
                                                                                                                       gTCTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGgcg                           >  2:3912493/1‑150 (MQ=255)
                                                                                                                         cTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCt                         <  2:4140426/150‑1 (MQ=255)
                                                                                                                         cTGGAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCt                         <  1:3409014/150‑1 (MQ=255)
                                                                                                                            gAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGAc                      <  1:2819131/150‑1 (MQ=255)
                                                                                                                            gAAGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGAc                      <  1:730567/150‑1 (MQ=255)
                                                                                                                              aGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACgg                    >  2:3593931/1‑150 (MQ=255)
                                                                                                                              aGGTAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACgg                    >  2:3758738/1‑150 (MQ=255)
                                                                                                                                 tAAACTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCaa                 >  2:1482021/1‑150 (MQ=255)
                                                                                                                                  aaaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCCAc                >  2:3528173/1‑150 (MQ=255)
                                                                                                                                  aaaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAAc                >  1:604358/1‑150 (MQ=255)
                                                                                                                                   aaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACg               >  1:3314221/1‑150 (MQ=255)
                                                                                                                                   aaCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACg               <  1:1738161/150‑1 (MQ=255)
                                                                                                                                    aCTGGTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGc              >  2:717508/1‑150 (MQ=255)
                                                                                                                                       ggTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACt           >  2:2089083/1‑150 (MQ=255)
                                                                                                                                       ggTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGACAACGCACt           >  1:2508273/1‑150 (MQ=255)
                                                                                                                                        gTTCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTg          >  1:2626041/1‑150 (MQ=255)
                                                                                                                                         ttCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGt         >  2:1350605/1‑150 (MQ=255)
                                                                                                                                         ttCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGt         >  2:2951437/1‑150 (MQ=255)
                                                                                                                                          tCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTggt        <  2:2650715/150‑3 (MQ=255)
                                                                                                                                          tCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGtt        <  2:4106462/150‑1 (MQ=255)
                                                                                                                                          tCAGCAGTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGtt        >  1:2774778/1‑150 (MQ=255)
                                                                                                                                           cagcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAACTCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTc       <  1:1088303/150‑1 (MQ=255)
                                                                                                                                            agcagTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTcc      >  1:1752615/1‑150 (MQ=255)
                                                                                                                                               agTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGcc   >  2:3317201/1‑150 (MQ=255)
                                                                                                                                                gTTAACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGCCa  >  1:2270503/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AGGCGGCGTGGAAATCGAAAAAGTGGCGGAAGAAACTCCGCACCTGATCCATAAAGTTGCGCTTGATCCGCTGACTGGCCCGATGCCGTATCAGGGACGCGAGCTGGCGTTCAAACTGGGTCTGGAAGGTAAACTGGTTCAGCAGTTCACCAAAATCTTCATGGGCCTGGCGACCATTTTCCTGGAGCGCGACCTGGCGTTGATCGAAATCAACCCGCTGGTCATCACCAAACAGGGCGATCTGATTTGCCTCGACGGCAAACTGGGCGCTGACGGCAACGCACTGTTCCGCCA  >  CP009273/758853‑759146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑