Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 825,641 G→T 40.5% A153A (GCC→GCA)  ybiH ← DUF1956 domain‑containing tetR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273825,6410GT40.5% 292.2 / 297.5 252A153A (GCC→GCA) ybiHDUF1956 domain‑containing tetR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (74/76);  new base T (58/44);  total (132/120)
Fisher's exact test for biased strand distribution p-value = 2.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.20e-01

Only 100 of 259 total aligned reads displayed.

ATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCT  >  CP009273/825492‑825790
                                                                                                                                                     |                                                                                                                                                     
     ttCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAAt                                                                                                                                                  >  2:2761916/1‑150 (MQ=255)
     ttCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAAt                                                                                                                                                  <  2:3743580/150‑1 (MQ=255)
       cGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCa                                                                                                                                                >  1:3886463/1‑150 (MQ=255)
        ggTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAg                                                                                                                                               >  1:635073/1‑150 (MQ=255)
        ggTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAg                                                                                                                                               <  1:3703356/150‑1 (MQ=255)
        ggTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAg                                                                                                                                               <  1:3958605/150‑1 (MQ=255)
           cTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCg                                                                                                                                            <  1:2940445/150‑1 (MQ=255)
            ttttCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCgt                                                                                                                                           >  1:3434491/1‑150 (MQ=255)
               tcttcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTc                                                                                                                                        <  2:3447540/150‑1 (MQ=255)
                  tcATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAgg                                                                                                                                     >  1:1626868/1‑150 (MQ=255)
                       gtaCGCGGTCCAGCCGGTACGTAACAGAATTGGTTCTTTGCCAAGACTGAACGCCAGAATCTGTCCAATCAACGCATGGGTTTGAAGGATCTTGCGGGTGTCATTGGCGTCGCAGCCGTTCCAGGAGGCAATCAGGCTTGTCAGGGGGCt                                                                                                                                <  2:736365/148‑1 (MQ=255)
                       gAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCt                                                                                                                                >  2:1221087/1‑150 (MQ=255)
                           gcgGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTgtgt                                                                                                                            <  1:432546/150‑1 (MQ=255)
                             ggTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCTTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAg                                                                                                                          >  2:2641234/1‑150 (MQ=255)
                                     cGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTaa                                                                                                                  <  1:2246189/150‑1 (MQ=255)
                                       gTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATc                                                                                                                >  1:1772937/1‑150 (MQ=255)
                                          cGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                                             >  1:858691/1‑150 (MQ=255)
                                          cGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                                             >  2:1433788/1‑150 (MQ=255)
                                          cGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCAcc                                                                                                             <  1:2775328/150‑1 (MQ=255)
                                           gTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCt                                                                                                            <  1:1089941/150‑1 (MQ=255)
                                                aGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCg                                                                                                       >  2:1982398/1‑150 (MQ=255)
                                                aGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCg                                                                                                       >  2:1982608/1‑150 (MQ=255)
                                                 gAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGt                                                                                                      <  2:3915197/150‑1 (MQ=255)
                                                    ttGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCa                                                                                                   <  1:1866823/150‑1 (MQ=255)
                                                    ttGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCa                                                                                                   >  2:3651672/1‑150 (MQ=255)
                                                    ttGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCa                                                                                                   <  1:2444636/150‑1 (MQ=255)
                                                      gTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCAcc                                                                                                 >  1:267444/1‑150 (MQ=255)
                                                       tttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCa                                                                                                >  1:1492657/1‑150 (MQ=255)
                                                       tttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCa                                                                                                >  1:1946260/1‑150 (MQ=255)
                                                        ttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAg                                                                                               <  1:240363/150‑1 (MQ=255)
                                                        ttCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAg                                                                                               >  2:2261525/1‑150 (MQ=255)
                                                         tCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAgg                                                                                              >  2:4050401/1‑150 (MQ=255)
                                                          cTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAggt                                                                                             <  2:516779/150‑1 (MQ=255)
                                                             tGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAggtggt                                                                                          <  2:2278701/150‑1 (MQ=255)
                                                              gCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTa                                                                                         <  2:3034393/150‑1 (MQ=255)
                                                               ccAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAg                                                                                        <  1:770853/150‑1 (MQ=255)
                                                               ccAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAg                                                                                        <  1:3338496/150‑1 (MQ=255)
                                                               ccAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAg                                                                                        >  2:3962436/1‑150 (MQ=255)
                                                                     cGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGcc                                                                                  <  1:3503567/150‑1 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 >  2:1503049/1‑150 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGGTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 >  2:4085284/1‑150 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 <  2:20268/150‑1 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 >  2:3339352/1‑150 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 >  1:1641186/1‑150 (MQ=255)
                                                                      ggAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCg                                                                                 >  2:3004351/1‑150 (MQ=255)
                                                                        aaCGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTg                                                                               >  2:815958/1‑150 (MQ=255)
                                                                         aCGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTgg                                                                              <  1:3649112/150‑1 (MQ=255)
                                                                          cGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTggg                                                                             >  2:1590302/1‑150 (MQ=255)
                                                                          cGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTggg                                                                             >  2:581827/1‑150 (MQ=255)
                                                                            ccAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgag                                                                           >  2:3124047/1‑150 (MQ=255)
                                                                            ccAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgag                                                                           >  2:3123926/1‑150 (MQ=255)
                                                                             cAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgaga                                                                          <  2:2949417/150‑1 (MQ=255)
                                                                              aGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgagag                                                                         >  1:3271701/1‑150 (MQ=255)
                                                                               gAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGgagaga                                                                        <  1:2881618/150‑1 (MQ=255)
                                                                                  tctcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgct                                                                     <  1:2699812/150‑1 (MQ=255)
                                                                                   ctcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgctg                                                                    <  1:3510010/150‑1 (MQ=255)
                                                                                   ctcACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAgctg                                                                    <  1:3146806/150‑1 (MQ=255)
                                                                                        cAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCAc                                                               >  1:996180/1‑150 (MQ=255)
                                                                                             aaCGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGgag                                                          <  2:3517672/150‑1 (MQ=255)
                                                                                             aaCGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGgag                                                          >  1:4019958/1‑150 (MQ=255)
                                                                                             aaCGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGgag                                                          <  2:2703674/150‑1 (MQ=255)
                                                                                               cGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGAt                                                        <  1:3339104/150‑1 (MQ=255)
                                                                                                     ggTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACtt                                                  <  1:1822166/150‑1 (MQ=255)
                                                                                                     ggTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACtt                                                  <  1:3056631/150‑1 (MQ=255)
                                                                                                        aTGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCt                                               >  1:2150242/1‑150 (MQ=255)
                                                                                                          gAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGa                                             <  2:319147/150‑1 (MQ=255)
                                                                                                            aGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAgg                                           >  1:1170323/1‑150 (MQ=255)
                                                                                                             ggATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGt                                          <  2:1540868/150‑1 (MQ=255)
                                                                                                              gATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                         <  2:941785/150‑1 (MQ=255)
                                                                                                              gATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                         >  2:2251199/1‑150 (MQ=255)
                                                                                                              gATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                         >  1:2589185/1‑150 (MQ=255)
                                                                                                              gATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGtt                                         >  1:4070547/1‑150 (MQ=255)
                                                                                                                 cATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGAc                                      >  2:438141/1‑150 (MQ=255)
                                                                                                                  aTGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACg                                     >  1:1117268/1‑150 (MQ=255)
                                                                                                                          gtCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATc                             <  2:2755523/150‑1 (MQ=255)
                                                                                                                             aTTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTg                          <  1:2568728/150‑1 (MQ=255)
                                                                                                                             aTTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTg                          >  2:643498/1‑150 (MQ=255)
                                                                                                                                ggCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGt                       <  2:2085438/150‑1 (MQ=255)
                                                                                                                                 gCGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTg                      <  1:2773062/150‑1 (MQ=255)
                                                                                                                                 gCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTg                      <  2:1736722/150‑1 (MQ=255)
                                                                                                                                  cGTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGa                     <  2:2143863/150‑1 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                    >  1:541181/1‑150 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                    >  1:3901670/1‑150 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                    <  2:491059/150‑1 (MQ=255)
                                                                                                                                   gTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGag                    <  2:3073793/150‑1 (MQ=255)
                                                                                                                                    tCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGagc                   <  1:1357602/150‑1 (MQ=255)
                                                                                                                                      gCAGCCGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGagcag                 <  1:4074431/150‑1 (MQ=255)
                                                                                                                                        aGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCt               >  2:2876914/1‑150 (MQ=255)
                                                                                                                                        aGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCt               <  2:111228/150‑1 (MQ=255)
                                                                                                                                          ccGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTa             <  1:133078/150‑1 (MQ=255)
                                                                                                                                           cGGTCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTaa            >  1:2716627/1‑150 (MQ=255)
                                                                                                                                           cGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATACACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTaa            <  1:582795/150‑1 (MQ=255)
                                                                                                                                             gTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATc          >  2:1489665/1‑150 (MQ=255)
                                                                                                                                              tCCAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCa         >  2:2391408/1‑150 (MQ=255)
                                                                                                                                               ccAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCAt        <  1:2270790/150‑1 (MQ=255)
                                                                                                                                                cAGGCTGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATg       <  2:1853041/150‑1 (MQ=255)
                                                                                                                                                 aGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGt      >  1:3028136/1‑150 (MQ=255)
                                                                                                                                                  ggcggcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGtt     <  2:804826/150‑1 (MQ=255)
                                                                                                                                                     ggcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCt  >  2:3763711/1‑150 (MQ=255)
                                                                                                                                                     ggcAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCt  <  1:1193123/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATCAGTTCGGTCTTTTCTTCATCGAACGCGGTCCAGCCGGTACGTAACAGAATTGTTTCTTTGCCAAGACGGAACGCCAGAATCTCACCAATCAACGCATGGGTATGAAGGATCATGCGGGTGTCATTGGCGTCGCAGCCGGTCCAGGCGGCAATCAGGCGTGTCAGGTGGCTGTGTAGCGGACTAATCACCTGTTCGTGCACCAGGTGGTAGGCTGCCGTGGGAGAGAGCTGCTCACGGGAGATAAACTTGCTGAGGTTGACGGTGTCATCCTGGGTGAGCAGCTTAATCATGTTCCT  >  CP009273/825492‑825790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑