Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 832,445 G→T 100% P213P (CCG→CCT)  ybiC → putative dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273832,4450GT97.2% 856.8 / 12.8 254P213P (CCG→CCT) ybiCputative dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (2/5);  new base T (115/132);  total (117/137)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 265 total aligned reads displayed.

GTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACA  >  CP009273/832298‑832591
                                                                                                                                                   |                                                                                                                                                  
gTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctac                                                                                                                                                  >  1:2939161/1‑150 (MQ=255)
gTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctac                                                                                                                                                  <  1:1368587/150‑1 (MQ=255)
gTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctac                                                                                                                                                  >  1:3254077/1‑150 (MQ=255)
 ttCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCctacg                                                                                                                                                 >  1:4044109/1‑150 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCtacgac                                                                                                                                               <  1:2541668/150‑1 (MQ=255)
   cTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCtacgac                                                                                                                                               <  1:1305184/150‑1 (MQ=255)
     gtgtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCa                                                                                                                                             <  2:1331056/150‑1 (MQ=255)
     gtgtgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCa                                                                                                                                             >  1:3778758/1‑150 (MQ=255)
        tgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATc                                                                                                                                          >  2:1796109/1‑150 (MQ=255)
        tgGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATc                                                                                                                                          >  2:32642/1‑150 (MQ=255)
         ggTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATcc                                                                                                                                         <  1:1603837/150‑1 (MQ=255)
         ggTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATcc                                                                                                                                         <  1:895704/150‑1 (MQ=255)
         ggTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATcc                                                                                                                                         >  1:3907644/1‑150 (MQ=255)
          gTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcg                                                                                                                                        >  1:1302567/1‑150 (MQ=255)
            tttCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCcggc                                                                                                                                      <  1:4166403/150‑1 (MQ=255)
               cccTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGt                                                                                                                                   >  1:3123463/1‑150 (MQ=255)
                 cTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTaa                                                                                                                                 >  2:1934339/1‑150 (MQ=255)
                   cGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATg                                                                                                                               >  2:1426424/1‑150 (MQ=255)
                     tAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCa                                                                                                                             >  2:2746676/1‑150 (MQ=255)
                      aaaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAg                                                                                                                            <  2:3543822/150‑1 (MQ=255)
                      aaaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAg                                                                                                                            <  2:2254429/150‑1 (MQ=255)
                      aaaGATAATTACCCGCTCTAGCTAGATTACTCCACCAGCGCCATTGCATTTGCCAAAACCCGCGTCGCCTGGCATAAAGTCGTCCCCGTGACGCCAGCTTGCCAGATTGACGTTAACGGCGTGCCTACGACCAAACCGGCGGTAAAGCAg                                                                                                                            >  2:3770042/1‑150 (MQ=255)
                       aaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAgg                                                                                                                           <  2:1097593/150‑1 (MQ=255)
                       aaGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAgg                                                                                                                           >  1:4122706/1‑150 (MQ=255)
                         gATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGCTTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAg                                                                                                                         <  2:463944/150‑1 (MQ=255)
                           tAATTTCCCTCTGTTGCTTGATTACGCCACCAGCGCCAGTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTc                                                                                                                       <  2:946632/150‑1 (MQ=255)
                           tAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTCACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTc                                                                                                                       >  2:3339859/1‑150 (MQ=255)
                           tAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTc                                                                                                                       >  2:3888059/1‑150 (MQ=255)
                           tAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTc                                                                                                                       <  2:877895/150‑1 (MQ=255)
                            aaTTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCg                                                                                                                      >  1:191698/1‑150 (MQ=255)
                             aTTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGc                                                                                                                     <  2:3486538/150‑1 (MQ=255)
                              tttCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGcc                                                                                                                    <  1:1784476/150‑1 (MQ=255)
                                    gCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTggg                                                                                                              >  1:660098/1‑150 (MQ=255)
                                    gCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTggg                                                                                                              >  2:2804340/1‑150 (MQ=255)
                                    gCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTggg                                                                                                              >  1:3592588/1‑150 (MQ=255)
                                     cTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGt                                                                                                             >  2:2008491/1‑150 (MQ=255)
                                      tGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCATGAGTCGCCTTTGGGtt                                                                                                            <  2:439234/150‑1 (MQ=255)
                                      tGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGtt                                                                                                            >  1:341268/1‑150 (MQ=255)
                                      tGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGtt                                                                                                            <  1:457208/150‑1 (MQ=255)
                                       gTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTc                                                                                                           >  1:208483/1‑150 (MQ=255)
                                            ttGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGt                                                                                                      >  2:3490955/1‑150 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                     <  2:3455600/150‑1 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                     >  1:501047/1‑150 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                     <  2:70544/150‑1 (MQ=255)
                                             tGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGtt                                                                                                     <  1:108428/150‑1 (MQ=255)
                                                 tACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGAcc                                                                                                 >  2:3020570/1‑150 (MQ=255)
                                                       accaGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGcc                                                                                           >  2:409066/1‑150 (MQ=255)
                                                          aGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGaa                                                                                        <  2:3667282/150‑1 (MQ=255)
                                                           gcgcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAAc                                                                                       <  2:4004238/150‑1 (MQ=255)
                                                             gcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATTTAGGCGTCCACGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGTGTTCGCTGTTGACCTTTGCCGAACAt                                                                                     <  1:1003471/150‑1 (MQ=255)
                                                             gcCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACAt                                                                                     <  1:1003497/150‑1 (MQ=255)
                                                               cATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaa                                                                                   >  2:1741108/1‑150 (MQ=255)
                                                               cATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATaa                                                                                   >  1:1511335/1‑150 (MQ=255)
                                                                  tGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAgg                                                                                >  1:972017/1‑150 (MQ=255)
                                                                   gCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGc                                                                               >  1:3808662/1‑150 (MQ=255)
                                                                   gCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGc                                                                               >  2:3048716/1‑150 (MQ=255)
                                                                     aTTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTa                                                                             >  1:745929/1‑150 (MQ=255)
                                                                          gCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGccc                                                                        <  2:973907/150‑1 (MQ=255)
                                                                          gCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGccc                                                                        <  1:1716903/150‑1 (MQ=255)
                                                                           cAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCt                                                                       >  2:2272491/1‑150 (MQ=255)
                                                                            aaaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCtt                                                                      <  2:2012306/150‑1 (MQ=255)
                                                                            aaaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCtt                                                                      <  1:120429/150‑1 (MQ=255)
                                                                            aaaaCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCtt                                                                      <  2:2461250/150‑1 (MQ=255)
                                                                               aCCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCa                                                                   <  1:2321598/150‑1 (MQ=255)
                                                                                 ccGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGc                                                                 <  2:1865191/150‑1 (MQ=255)
                                                                                     gTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtg                                                             >  1:3227710/1‑150 (MQ=255)
                                                                                        gCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGGGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtgtgt                                                          >  1:2292867/1‑150 (MQ=255)
                                                                                         ccTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGAtgtgtg                                                         <  1:3329114/150‑1 (MQ=255)
                                                                                          cTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGa                                                        >  1:2461223/1‑150 (MQ=255)
                                                                                           tGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGaa                                                       >  2:1698023/1‑150 (MQ=255)
                                                                                               aTAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTc                                                   >  1:526616/1‑150 (MQ=255)
                                                                                               aTAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTc                                                   >  2:1133089/1‑150 (MQ=255)
                                                                                                tAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCt                                                  >  1:2609131/1‑150 (MQ=255)
                                                                                                       gTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCggg                                           >  2:3715821/1‑150 (MQ=255)
                                                                                                         ccccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgc                                         <  2:3924058/150‑1 (MQ=255)
                                                                                                         ccccGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGgc                                         <  2:3758457/150‑1 (MQ=255)
                                                                                                             gTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCtt                                     >  1:222089/1‑150 (MQ=255)
                                                                                                             gTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCtt                                     >  2:2986295/1‑150 (MQ=255)
                                                                                                              tGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCttt                                    <  2:2448110/150‑1 (MQ=255)
                                                                                                              tGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCttt                                    >  2:1446158/1‑150 (MQ=255)
                                                                                                                   ccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggc                               >  1:2506839/1‑150 (MQ=255)
                                                                                                                   ccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggc                               >  1:3054052/1‑150 (MQ=255)
                                                                                                                   ccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggc                               >  2:263718/1‑150 (MQ=255)
                                                                                                                   ccAGGTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCcggc                               <  2:3949934/150‑1 (MQ=255)
                                                                                                                      ggTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGt                            <  2:2432339/150‑1 (MQ=255)
                                                                                                                      ggTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGt                            <  1:186150/150‑1 (MQ=255)
                                                                                                                       gTTGCCTGATTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTa                           <  2:1759242/150‑1 (MQ=255)
                                                                                                                            cTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacg                      <  2:1497321/150‑1 (MQ=255)
                                                                                                                               aTTGACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAacgacg                   <  2:1889740/150‑1 (MQ=255)
                                                                                                                                  gACGTTAACGGCGTGCCTACGACCAATCCGGCGGTATTTCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCAt                <  2:4137948/150‑1 (MQ=255)
                                                                                                                                  gACGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCAt                <  1:133502/150‑1 (MQ=255)
                                                                                                                                   aCGTTAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATc               <  2:544904/150‑1 (MQ=255)
                                                                                                                                       tAACGGCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTAGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGa           <  2:623732/150‑1 (MQ=255)
                                                                                                                                           ggCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACg       <  2:1297607/150‑1 (MQ=255)
                                                                                                                                           ggCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACg       <  2:84575/150‑1 (MQ=255)
                                                                                                                                           ggCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACg       >  1:2554564/1‑150 (MQ=255)
                                                                                                                                            gCGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGt      <  2:2869554/150‑1 (MQ=255)
                                                                                                                                             cGTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGtt     <  1:209227/150‑1 (MQ=255)
                                                                                                                                              gTGCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTa    >  2:3169417/1‑150 (MQ=255)
                                                                                                                                                gCCTACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACa  <  1:621723/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GTTCTGTGTGGTTTTCCCTCGTAAAGATAATTTCCCGCTGTTGCTTGATTACGCCACCAGCGCCATTGCATTTGGCAAAACCCGCGTCGCCTGGCATAAAGGCGTCCCCGTGCCGCCAGGTTGCCTGATTGACGTTAACGGCGTGCCGACGACCAATCCGGCGGTAATGCAGGAGTCGCCGTTGGGTTCGCTGTTGACCTTTGCCGAACATAAAGGCTACGCCCTTGCAGCGATGTGTGAAATTCTTGGCGGGGCGCTTTCCGGCGGTAAAACGACGCATCAGGAAACGTTACA  >  CP009273/832298‑832591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑