Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 925,160 G→T 100% S420* (TCA→TAA)  cydD ← fused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273925,1600GT97.4% 792.7 / 10.1 233S420* (TCA→TAA) cydDfused glutathione, cysteine exporter subunits of ABC superfamily: membrane component/ATP‑binding component
Reads supporting (aligned to +/- strand):  ref base G (6/0);  new base T (107/120);  total (113/120)
Fisher's exact test for biased strand distribution p-value = 1.21e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 235 total aligned reads displayed.

GGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTA  >  CP009273/925013‑925306
                                                                                                                                                   |                                                                                                                                                  
ggTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTAt                                                                                                                                                  <  2:17954/150‑1 (MQ=255)
 gTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCattatt                                                                                                                                                 <  1:885941/150‑1 (MQ=255)
 gTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCattatt                                                                                                                                                 <  1:3630831/150‑1 (MQ=255)
  tAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTc                                                                                                                                                >  1:1305335/1‑150 (MQ=255)
  tAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTc                                                                                                                                                <  1:2345311/150‑1 (MQ=255)
   aGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCt                                                                                                                                               <  2:910443/150‑1 (MQ=255)
    gAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTg                                                                                                                                              >  1:3832340/1‑150 (MQ=255)
    gAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAAGTGTGGGTTTTGCCCAACCCCGGAGAGATGTTTACGCCATTATTCTg                                                                                                                                              >  2:3451587/1‑150 (MQ=255)
    gAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTAACCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTg                                                                                                                                              >  2:3451415/1‑150 (MQ=255)
     aaaCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTgg                                                                                                                                             >  2:1175842/1‑150 (MQ=255)
      aaCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGt                                                                                                                                            <  1:728468/150‑1 (MQ=255)
      aaCTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGt                                                                                                                                            >  2:458154/1‑150 (MQ=255)
        cTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGa                                                                                                                                          <  1:1607388/150‑1 (MQ=255)
        cTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGa                                                                                                                                          <  1:1607465/150‑1 (MQ=255)
         tCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGAt                                                                                                                                         <  1:3121900/150‑1 (MQ=255)
          cGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATa                                                                                                                                        <  1:4152451/150‑1 (MQ=255)
              gACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATc                                                                                                                                    >  2:254635/1‑150 (MQ=255)
               aCCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATcg                                                                                                                                   <  2:880167/150‑1 (MQ=255)
               aCCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATcg                                                                                                                                   >  1:2398173/1‑150 (MQ=255)
                cccAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATcgc                                                                                                                                  >  2:4053859/1‑150 (MQ=255)
                cccAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATcgc                                                                                                                                  <  2:2188616/150‑1 (MQ=255)
                  cAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGt                                                                                                                                <  2:248746/150‑1 (MQ=255)
                  cAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGt                                                                                                                                >  1:4117896/1‑150 (MQ=255)
                   aGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTa                                                                                                                               >  2:2901104/1‑150 (MQ=255)
                     gCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTCGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAAt                                                                                                                             <  2:1907173/150‑1 (MQ=255)
                      cGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAAtt                                                                                                                            <  1:2979616/150‑1 (MQ=255)
                      cGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAAtt                                                                                                                            <  1:3279738/150‑1 (MQ=255)
                       gTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTc                                                                                                                           >  1:84007/1‑150 (MQ=255)
                         tATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTa                                                                                                                         <  2:3896449/150‑1 (MQ=255)
                         tATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTa                                                                                                                         >  1:3233822/1‑150 (MQ=255)
                          aTCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTAt                                                                                                                        >  2:1507609/1‑150 (MQ=255)
                           tCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATc                                                                                                                       <  1:1116746/150‑1 (MQ=255)
                           tCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATc                                                                                                                       >  1:2272671/1‑150 (MQ=255)
                             cAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATccc                                                                                                                     <  1:1585818/150‑1 (MQ=255)
                             cAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATccc                                                                                                                     <  1:2643995/150‑1 (MQ=255)
                             cAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATccc                                                                                                                     <  2:795757/150‑1 (MQ=255)
                              aGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCg                                                                                                                    <  2:3764160/150‑1 (MQ=255)
                                  ctgctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGa                                                                                                                >  2:2881129/1‑150 (MQ=255)
                                  ctgctTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGa                                                                                                                <  1:2303472/150‑1 (MQ=255)
                                      ttGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCg                                                                                                            >  1:3492812/1‑150 (MQ=255)
                                       tGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGt                                                                                                           >  2:3972027/1‑150 (MQ=255)
                                        gTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTa                                                                                                          <  2:2716364/150‑1 (MQ=255)
                                         tAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAg                                                                                                         <  1:905710/150‑1 (MQ=255)
                                          aaTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGc                                                                                                        <  2:2682898/150‑1 (MQ=255)
                                           aTTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTGTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCg                                                                                                       >  2:72390/1‑150 (MQ=255)
                                                 gTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCt                                                                                                 <  1:210998/150‑1 (MQ=255)
                                                     gCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGata                                                                                             <  1:4011778/150‑1 (MQ=255)
                                                     gCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGata                                                                                             >  1:3015274/1‑150 (MQ=255)
                                                        ggCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATga                                                                                          <  2:3733065/150‑1 (MQ=255)
                                                             cAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaa                                                                                     >  1:2664856/1‑150 (MQ=255)
                                                              aGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaaa                                                                                    >  2:3008627/1‑150 (MQ=255)
                                                              aGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaaa                                                                                    <  1:551241/150‑1 (MQ=255)
                                                               ggTAGCGCCAGTATTACGTTATCAAGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGTAGAGATGTTTACGCCATTATTCTGGTGATAAATAGAGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGaaaa                                                                                   <  2:1701141/150‑1 (MQ=255)
                                                                gTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAAc                                                                                  <  1:1997025/150‑1 (MQ=255)
                                                                   gcgcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAg                                                                               <  1:2537690/150‑1 (MQ=255)
                                                                     gcCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGaa                                                                             <  2:1592161/150‑1 (MQ=255)
                                                                        agtagtACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAgc                                                                          >  2:2238157/1‑150 (MQ=255)
                                                                        agtagtACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAgc                                                                          <  2:783384/150‑1 (MQ=255)
                                                                        agtagtACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAgc                                                                          <  2:1199205/150‑1 (MQ=255)
                                                                           agtaCGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGcgcg                                                                       >  2:1913151/1‑150 (MQ=255)
                                                                             taCGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGcgcgat                                                                     >  2:3617719/1‑148 (MQ=255)
                                                                                   aTCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTcagcag                                                               >  1:677060/1‑150 (MQ=255)
                                                                                     cccGCATTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTg                                                             <  2:2809893/150‑1 (MQ=255)
                                                                                      ccGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGa                                                            >  1:3736315/1‑150 (MQ=255)
                                                                                      ccGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGa                                                            >  1:3462324/1‑150 (MQ=255)
                                                                                       cGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAg                                                           <  2:1284421/150‑1 (MQ=255)
                                                                                       cGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAg                                                           >  1:2762737/1‑150 (MQ=255)
                                                                                           aTGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCttt                                                       >  2:1148847/1‑150 (MQ=255)
                                                                                             gTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTa                                                     <  1:1374428/150‑1 (MQ=255)
                                                                                               tgctgcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTAcc                                                   >  2:809638/1‑150 (MQ=255)
                                                                                               tgctgcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTAcc                                                   <  1:1911732/150‑1 (MQ=255)
                                                                                                 ctgcCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTg                                                 <  1:1751372/150‑1 (MQ=255)
                                                                                                    ccGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAAc                                              >  1:1069220/1‑150 (MQ=255)
                                                                                                    ccGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAAc                                              <  2:2917530/150‑1 (MQ=255)
                                                                                                      ggTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCg                                            <  1:72390/150‑1 (MQ=255)
                                                                                                      ggTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCg                                            >  2:1315961/1‑150 (MQ=255)
                                                                                                        tAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCt                                          <  1:4106012/150‑1 (MQ=255)
                                                                                                         aaTTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTg                                         <  1:1804934/150‑1 (MQ=255)
                                                                                                         aaTTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATACTATCCCGTTGATTCGTAGCGATCCCTGATAGGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTg                                         >  2:421267/1‑150 (MQ=255)
                                                                                                                ggTTTTGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGaccaa                                  <  1:537513/150‑1 (MQ=255)
                                                                                                                     tGCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACcaaccaac                             >  1:1765569/1‑150 (MQ=255)
                                                                                                                      gCCCAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAaca                            <  2:4014977/150‑1 (MQ=255)
                                                                                                                        ccAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACAcc                          >  2:1674179/1‑150 (MQ=255)
                                                                                                                        ccAACCCAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACAcc                          <  1:2789936/150‑1 (MQ=255)
                                                                                                                            cccAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCAc                      <  2:3126567/150‑1 (MQ=255)
                                                                                                                              cAGGAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGt                    <  1:2992338/150‑1 (MQ=255)
                                                                                                                                ggAGAGATGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTg                  <  2:2590352/150‑1 (MQ=255)
                                                                                                                                 gagagaTGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTgg                 >  1:2840475/1‑150 (MQ=255)
                                                                                                                                   gagaTGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGcc               >  2:3142265/1‑150 (MQ=255)
                                                                                                                                   gagaTGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGcc               <  1:2739644/150‑1 (MQ=255)
                                                                                                                                      aTGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgc            >  2:1967541/1‑150 (MQ=255)
                                                                                                                                       tGTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgct           <  1:1162506/150‑1 (MQ=255)
                                                                                                                                        gTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgctg          >  2:3113158/1‑150 (MQ=255)
                                                                                                                                        gTTTACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCctgctg          >  2:3678269/1‑150 (MQ=255)
                                                                                                                                         tttACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTgg         >  2:2432821/1‑150 (MQ=255)
                                                                                                                                          ttACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGc        >  2:2737913/1‑150 (MQ=255)
                                                                                                                                          ttACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGc        >  2:2127901/1‑150 (MQ=255)
                                                                                                                                           tACGCCATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCa       >  2:640016/1‑150 (MQ=255)
                                                                                                                                               ccATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagt   >  1:1435048/1‑150 (MQ=255)
                                                                                                                                                cATTATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCaaagta  >  2:584167/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTAGAAACTCGCTGACCCAGGCGTTATCCAGCGCTGCTTGTAATTCTTGTTCGCTGGCATCAGGTCGCGCCAGTAGTACGTTATCCCGCAATGTTGCTGCCGGTAATTGTGGGTTTTGCCCAACCCAGGAGAGATGTTTACGCCATGATTCTGGTGATAAATCGCGTAATTCTATCCCGTTGATTCGTAGCGATCCCTGATATGAGAGAAAACCAGAAAGCGCGTTCAGCAGTGAGCTTTTACCTGAACCGCTGCGACCAACCAACACCGCACGTTGGCCTGCTGGCAAAGTA  >  CP009273/925013‑925306

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑