Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,277,786 G→A 100% D823N (GAC→AAC)  narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,277,7860GA97.7% 911.5 / 7.3 259D823N (GAC→AAC) narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (1/5);  new base A (127/125);  total (128/131)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 259 total aligned reads displayed.

TCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAA  >  CP009273/1277640‑1277932
                                                                                                                                                  |                                                                                                                                                  
tCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACt                                                                                                                                                 <  1:260938/150‑1 (MQ=255)
tCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACt                                                                                                                                                 <  1:4049634/150‑1 (MQ=255)
 ccGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTg                                                                                                                                                <  1:3000767/150‑1 (MQ=255)
   gTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTggg                                                                                                                                              >  2:1096874/1‑150 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                       <  2:2883108/150‑1 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                       >  1:1371809/1‑150 (MQ=255)
          gAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTa                                                                                                                                       >  1:813000/1‑150 (MQ=255)
           aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                      >  1:236307/1‑150 (MQ=255)
           aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                      <  1:43575/150‑1 (MQ=255)
           aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTAc                                                                                                                                      >  2:120811/1‑150 (MQ=255)
             cACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaa                                                                                                                                    <  2:2161008/150‑1 (MQ=255)
              aCCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACaaa                                                                                                                                   <  2:4070031/150‑1 (MQ=255)
                cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGc                                                                                                                                 <  2:131824/150‑1 (MQ=255)
                cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGc                                                                                                                                 >  2:1563262/1‑150 (MQ=255)
                cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGc                                                                                                                                 <  1:2201048/150‑1 (MQ=255)
                  gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCa                                                                                                                               <  1:2522698/150‑1 (MQ=255)
                  gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCa                                                                                                                               >  2:1323368/1‑150 (MQ=255)
                  gTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCa                                                                                                                               <  1:2982377/150‑1 (MQ=255)
                       tATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATcgcg                                                                                                                          <  2:2691184/150‑1 (MQ=255)
                        aTTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCga                                                                                                                         >  1:74689/1‑150 (MQ=255)
                         ttCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCgaa                                                                                                                        <  1:936944/150‑1 (MQ=255)
                               catcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAAtt                                                                                                                  >  2:1337128/1‑150 (MQ=255)
                                 tcatTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATtct                                                                                                                <  2:3253090/150‑1 (MQ=255)
                                   atTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTcc                                                                                                              >  1:3343499/1‑150 (MQ=255)
                                    ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCg                                                                                                             <  1:872830/150‑1 (MQ=255)
                                    ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCg                                                                                                             <  1:1775213/150‑1 (MQ=255)
                                     tttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGa                                                                                                            <  1:2109289/150‑1 (MQ=255)
                                       tGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAg                                                                                                          >  2:3139963/1‑150 (MQ=255)
                                            aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc                                                                                                     <  2:1371438/150‑1 (MQ=255)
                                            aCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGc                                                                                                     <  2:3463686/150‑1 (MQ=255)
                                                 gACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCgg                                                                                                >  2:3785969/1‑150 (MQ=255)
                                                  aCCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGc                                                                                               >  2:153871/1‑150 (MQ=255)
                                                     tGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGTGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCAt                                                                                            >  1:636104/1‑150 (MQ=255)
                                                     tGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCAt                                                                                            >  1:1290067/1‑150 (MQ=255)
                                                      ggTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATc                                                                                           >  2:1482328/1‑150 (MQ=255)
                                                       gTACGAAACAGTCGACATGAATACTTCGGATTTTCATGCGTTTATTCAGCCGCTGTCTGCGGCGGTCGATCCGTCCTGGTTAGCGAATAGCATCTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCt                                                                                          <  2:2602897/150‑1 (MQ=255)
                                                         aCGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTgg                                                                                        >  2:3185275/1‑150 (MQ=255)
                                                          cGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTggg                                                                                       <  2:3718412/150‑1 (MQ=255)
                                                           gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGTGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt                                                                                      >  1:1356636/1‑150 (MQ=255)
                                                           gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt                                                                                      >  2:4110951/1‑150 (MQ=255)
                                                           gAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGt                                                                                      >  2:4110906/1‑150 (MQ=255)
                                                            aaaaaGACGACATGAATACTTCGGATATGCGTCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                     >  1:3505733/1‑150 (MQ=255)
                                                            aaaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTa                                                                                     <  1:732569/150‑1 (MQ=255)
                                                             aaaaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTaa                                                                                    <  2:2840614/150‑1 (MQ=255)
                                                               aaGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAg                                                                                  <  2:3953835/150‑1 (MQ=255)
                                                                aGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa                                                                                 >  2:2372469/1‑150 (MQ=255)
                                                                aGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGa                                                                                 >  2:2140230/1‑150 (MQ=255)
                                                                 gacgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaa                                                                                <  2:2324403/150‑1 (MQ=255)
                                                                 gacgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGaa                                                                                <  1:3525264/150‑1 (MQ=255)
                                                                     acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                                            <  2:3763560/150‑1 (MQ=255)
                                                                     acATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGAAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCg                                                                            >  1:1959338/1‑150 (MQ=255)
                                                                       aTGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGAc                                                                          <  1:3809472/150‑1 (MQ=255)
                                                                          aaTACTCCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATc                                                                       <  1:2851995/150‑1 (MQ=255)
                                                                                tCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACg                                                                 <  1:3479870/150‑1 (MQ=255)
                                                                                 cGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGc                                                                <  2:1665490/150‑1 (MQ=255)
                                                                                   gagatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTg                                                              <  2:2989679/147‑1 (MQ=255)
                                                                                   gATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTg                                                              >  2:2569532/1‑150 (MQ=255)
                                                                                    atatGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGc                                                             >  1:1241890/1‑150 (MQ=255)
                                                                                      atGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                           >  2:1360963/1‑150 (MQ=255)
                                                                                      atGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                           >  2:3971007/1‑150 (MQ=255)
                                                                                      atGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt                                                           >  2:256978/1‑150 (MQ=255)
                                                                                       tGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTa                                                          >  2:2514312/1‑150 (MQ=255)
                                                                                           tCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCa                                                      >  2:3664973/1‑150 (MQ=255)
                                                                                            ccGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg                                                     <  1:2061922/150‑1 (MQ=255)
                                                                                             cGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGc                                                    <  2:1877666/150‑1 (MQ=255)
                                                                                              gTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCa                                                   >  1:1103731/1‑150 (MQ=255)
                                                                                                  nttCACCCGCTGTCTGNGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGNGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGAc                                               >  2:155828/2‑150 (MQ=255)
                                                                                                    tCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc                                             >  2:2213573/1‑150 (MQ=255)
                                                                                                    tCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc                                             >  1:308086/1‑150 (MQ=255)
                                                                                                    tCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActc                                             >  1:702132/1‑150 (MQ=255)
                                                                                                     cACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGActct                                            >  1:3279865/1‑150 (MQ=255)
                                                                                                      aCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTg                                           <  1:3742120/150‑1 (MQ=255)
                                                                                                        ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                         <  2:2206409/150‑1 (MQ=255)
                                                                                                        ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGcc                                         <  1:1219704/150‑1 (MQ=255)
                                                                                                         cGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCg                                        >  1:1884861/1‑150 (MQ=255)
                                                                                                           cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt                                      >  1:1697463/1‑150 (MQ=255)
                                                                                                           cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCt                                      <  1:2568597/150‑1 (MQ=255)
                                                                                                            tGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTg                                     <  1:2993925/150‑1 (MQ=255)
                                                                                                              tCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGaa                                   <  1:2264835/150‑1 (MQ=255)
                                                                                                                tGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACt                                 <  1:3555510/150‑1 (MQ=255)
                                                                                                                  cggcggTCGATCCGGCCTGGGAAGCGAAACGCAACTGGGAATTATACAACGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGCCATCGTCACGCTGCCTCTCCAGCATGACTCTGCCGCTGAACTgg                               <  1:3986798/150‑1 (MQ=255)
                                                                                                                  cggcggTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTgg                               >  2:3393772/1‑150 (MQ=255)
                                                                                                                    gcggTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGgcg                             <  2:3645964/150‑1 (MQ=255)
                                                                                                                       gTCGATCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAg                          >  1:2554159/1‑150 (MQ=255)
                                                                                                                            tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCt                     <  2:3682345/150‑1 (MQ=255)
                                                                                                                            tCCGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCt                     >  2:451205/1‑150 (MQ=255)
                                                                                                                              cGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTgg                   <  2:3266708/150‑1 (MQ=255)
                                                                                                                              cGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTgg                   <  2:2069347/150‑1 (MQ=255)
                                                                                                                              cGGCCTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTgg                   <  2:104809/150‑1 (MQ=255)
                                                                                                                                 ccTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtg                <  2:1763668/150‑1 (MQ=255)
                                                                                                                                  cTGGGAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAtgt               >  2:1816120/1‑150 (MQ=255)
                                                                                                                                     ggAAGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGaa            >  1:2576925/1‑150 (MQ=255)
                                                                                                                                       aaGCGAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAg          >  1:3045441/1‑150 (MQ=255)
                                                                                                                                           gAAAAGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTg      >  1:2785985/1‑150 (MQ=255)
                                                                                                                                            aaaaGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg     <  1:4082915/150‑1 (MQ=255)
                                                                                                                                            aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg     <  1:561059/150‑1 (MQ=255)
                                                                                                                                            aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg     <  1:1082788/150‑1 (MQ=255)
                                                                                                                                            aaaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTgg     <  2:655503/150‑1 (MQ=255)
                                                                                                                                             aaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGa    >  2:2687557/1‑150 (MQ=255)
                                                                                                                                             aaaGCAACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGa    >  2:4169753/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATCTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGCCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCATGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAA  >  CP009273/1277640‑1277932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑