BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,282,439	A→G	6.0%	intergenic (+457/+83)	narI → / ← rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,282,439	0	A	G	6.0%	416.2 / 11.2	180	intergenic (+457/+83)	narI/rttR	nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand): ref base A (85/75); new base G (7/3); total (95/85)
Fisher's exact test for biased strand distribution p-value = 3.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

AGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAA  >  CP009273/1282293‑1282585

aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCAGCCGCAACCCTCACACGCTGACGCACGATCCCAAACATAGTc                                                                                                                                                 >  1:423745/1‑150 (MQ=14)

aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                                 >  2:4031061/1‑150 (MQ=12)

aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                                 <  2:810489/150‑1 (MQ=255)

aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                                                 <  1:3212009/150‑1 (MQ=255)

 gAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                                >  2:3191491/1‑150 (MQ=12)

 gAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCg                                                                                                                                                <  2:1021449/150‑1 (MQ=255)

  aaTATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACACGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                               >  2:3330539/1‑150 (MQ=255)

   atatTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCTCTCTCGCTGACGCTAGATACGAACCATAGTCGaa                                                                                                                                              >  2:1547595/1‑150 (MQ=14)

    tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGGGGCCAAGCGATCACGCAGACGCACGACACGAACCAAAGTAGaaa                                                                                                                                             >  1:746341/1‑149 (MQ=14)

      cttGATTGGGGATTAGTTTGAGTATTCCTGGGTTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                                           <  2:4171764/149‑1 (MQ=14)

            tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGTCGCTCGAGACCAACCGTAGTCGAAGCTTCACAt                                                                                                                                     >  2:2227348/1‑150 (MQ=14)

                gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATCCtt                                                                                                                                 >  2:2297430/1‑150 (MQ=255)

                    cGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                                             <  1:4031061/150‑1 (MQ=12)

                     gTTTGAGTATTCGGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                            <  1:2467740/150‑1 (MQ=255)

                     gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCACATccgaacacc                                                                                                                            >  2:2630282/1‑143 (MQ=255)

                     gTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCACGCTGACGCTCGAGCCGTACCATAGTCGAAGCTTCTCATCCTTACCCg                                                                                                                            >  1:1013704/1‑150 (MQ=14)

                        tGAGCATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                         <  1:3032446/150‑1 (MQ=255)

                        tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCACTCAAGCTGACGATAGATCCGAACAATACTCGAATCTAATCATCCTTCCCCGCag                                                                                                                         >  2:2268618/1‑149 (MQ=14)

                        tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                         <  2:3830418/150‑1 (MQ=255)

                        tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCCCaa                                                                                                                         >  2:3257513/1‑149 (MQ=255)

                          agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                                       <  1:842098/150‑1 (MQ=255)

                          agaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAAAGCTCTCTCGCGGACGCGAGAGCCGGACCGTAGTCGTAGCTTCTAATCCTTGCCCGCAggg                                                                                                                       >  1:1802201/1‑150 (MQ=14)

                           gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                                      >  2:2421959/1‑150 (MQ=255)

                             aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCATCTCATCCTTACCCGCATGGGCa                                                                                                                    >  2:3754617/1‑150 (MQ=255)

                             aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGAATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCa                                                                                                                    >  2:2326451/1‑150 (MQ=255)

                             aTTCCGGGGCTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCATTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGACGCTCGAGACGACCCTTAGTCGAAGCATCTCATACTTCCCCGCATGGGCa                                                                                                                    >  2:1992211/1‑150 (MQ=14)

                              ttCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCAACCCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCGTCTCATCCATCCCCGCATGGGCAg                                                                                                                   >  2:3794460/1‑150 (MQ=255)

                               tcgggggCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                                  <  2:483365/147‑1 (MQ=255)

                               tCCGGGGCTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                                  <  1:2749470/150‑1 (MQ=255)

                                 cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCTGACGCTCGAGCCGAACCGTATTAGAAGATTCTAATCCTTACACGCAATGGCAGAat                                                                                                                >  1:473880/1‑150 (MQ=14)

                                 cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCACGCAGACGCTCGAGCCCAACCAAAGTCGAACCATCACATCCATCCCCCCAAGGGCAGaag                                                                                                                >  1:4049602/1‑149 (MQ=14)

                                 cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCTGACGCTCGAGCCGAACCTTGGTCGAAGCATCTCATCCTTCCCCGCCTGGGCAGAat                                                                                                                >  2:1511350/1‑150 (MQ=11)

                                   gggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAatat                                                                                                              >  2:4098870/1‑150 (MQ=255)

                                   gggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATAACACAACACTCCGCGCTGCACCCTTCGTGAATTTGCCTGAGTCAAACAACGATCGCTCACTATCGATACGAACCTTAGTCGTAGCTTCTGATCCTTCCCCGGAGGGGCCGActag                                                                                                              >  2:4098858/1‑149 (MQ=2)

                                    ggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                             <  1:1263860/150‑1 (MQ=38)

                                     ctttttGAAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                                            <  2:1375819/148‑1 (MQ=37)

                                      tttaaggaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCATCACATCCGTCCCCGCATGGGCAGAATATTTg                                                                                                           >  2:3883699/8‑150 (MQ=11)

                                       ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCACTCTCGCTGGCGCTCGAGCCGAACCGTAGTCGAAGCTTATCATCCATCCCCGCATGGGGAGAataagtga                                                                                                          >  2:5825/1‑145 (MQ=11)

                                        tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAt                                                                                                         <  1:3897989/150‑1 (MQ=39)

                                        tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACACGCTGACCCTCGAGCCGAACCGTAGTCGAAGCTTATCATCCTTCCCCGCATGGGCCGAATAATTGAt                                                                                                         >  1:4136680/1‑150 (MQ=14)

                                           gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAAACATAGTCGAAGCTTCTCATCCATCCCCGCAGGGGCAGAATATTTGATTGc                                                                                                      >  2:3858628/1‑150 (MQ=14)

                                            aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGCCGGAGGCAACGCTCACTCGCTGGCGCTCGAGACGAAACTTATTCGAAGCATCACAACCATACCCGCATGGGGAGGATATTTGGTTggg                                                                                                     >  2:1764246/1‑148 (MQ=11)

                                               gcgATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGAt                                                                                                  <  1:1180554/148‑1 (MQ=39)

                                                    ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCt                                                                                             <  2:3485119/150‑1 (MQ=40)

                                                           ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGt                                                                                      >  1:2745245/1‑150 (MQ=255)

                                                           ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGt                                                                                      <  2:1198441/150‑1 (MQ=255)

                                                            gggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGtt                                                                                     >  2:3703355/1‑150 (MQ=255)

                                                              gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                                                                   <  1:1578394/150‑1 (MQ=255)

                                                               aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAg                                                                                  <  2:2680642/150‑1 (MQ=255)

                                                                 ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                                                                <  1:1644826/150‑1 (MQ=255)

                                                                  gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGATTAGCTTGAGAGTTCAgggc                                                                               >  2:3146450/1‑149 (MQ=255)

                                                                   aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGCATCTTTGATTGCGGATTCGCGTGAGAGTTCAGGGAc                                                                              >  2:204820/1‑150 (MQ=255)

                                                                   aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCGGACGCACGCGCCGAACCATGGTCGAAGCGTCTCATCCGTACCCCCCAGGGCCGGATAATTGATTGCGGATTCGGTTGGGAGTTCAGGGAc                                                                              >  1:3503436/1‑150 (MQ=11)

                                                                   aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGAc                                                                              <  1:318390/150‑1 (MQ=255)

                                                                     tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                                                            <  1:3883699/150‑1 (MQ=255)

                                                                      aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGGTTGAGAGGTCAGGGACttt                                                                           >  2:2945803/1‑150 (MQ=255)

                                                                       cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                                          <  1:3540813/150‑1 (MQ=255)

                                                                       cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                                          <  1:710057/150‑1 (MQ=255)

                                                                       cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACtttt                                                                          <  2:4064461/150‑1 (MQ=255)

                                                                        tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGGCGCTAGAGGCGAACCTTAGTCGAAGCGTCTCATACGTCCCCCCAAGGGCAGTATAATTTATTGCGGATTCGGTTGTGAGTTCAGGGACTTTTg                                                                         >  1:1722271/1‑150 (MQ=11)

                                                                          aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                                       <  1:1046592/150‑1 (MQ=255)

                                                                          aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                                       <  2:1410180/150‑1 (MQ=255)

                                                                             gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                                    <  2:3080008/150‑1 (MQ=255)

                                                                             gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                                    <  2:556373/150‑1 (MQ=255)

                                                                              cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                                                   <  1:436675/150‑1 (MQ=255)

                                                                              cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                                                                   <  1:436490/150‑1 (MQ=255)

                                                                               tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGA                                                                  <  2:881578‑M1/150‑1 (MQ=255)

                                                                                   cgcTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCGTAGGCGGAGCTTATCATCCTTCCCCGCAGGGGCAGAATATTTTATTGCGGCTTCGGGTGGGAGTTCAGGGACGTTTGAAAGTggtggt                                                              >  2:1589154/1‑150 (MQ=11)

                                                                                     cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAACCTTCCCCGCCTGGGCAGAATATTTTATTGCGGATTCGGTTGGTAGTTCAGGGACGTGTGAAAGGGCTGGTGG                                                            >  2:2856830‑M1/1‑150 (MQ=255)

                                                                                       tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                                          <  1:327259‑M1/150‑1 (MQ=255)

                                                                                         gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAAACATCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTAAGGGACGTTTGAAAGTGATGGTGGTGGG                                                        >  2:3033582‑M1/1‑150 (MQ=255)

                                                                                           ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGG                                                      <  1:3206037‑M1/150‑1 (MQ=255)

                                                                                            cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                                     <  2:3955333‑M1/150‑1 (MQ=255)

                                                                                            cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                                     <  2:3955091‑M1/150‑1 (MQ=255)

                                                                                            cTTCGGGTCGTTGCCTGCCGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCgtt                                                                                             >  2:135432/1‑108 (MQ=255)

                                                                                             ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                                    <  1:2249966‑M1/150‑1 (MQ=255)

                                                                                               cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATACTTACCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG                                                  >  2:3059950‑M1/1‑150 (MQ=255)

                                                                                                   tCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGGCGAACCTTAGTCGACGCTTCTCACCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                                              <  2:1206024/150‑1 (MQ=14)

                                                                                                   tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGACTATTTGATTTCGGATTCGCTTGAGCGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTa                                              >  1:623863/1‑150 (MQ=17)

                                                                                                     gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                                            <  1:2639014/150‑1 (MQ=255)

                                                                                                         ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTAATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGCAAGTGATGGTGGTGGGGGAAGGATTagtaagc                                        >  2:1880780‑M1/1‑143 (MQ=255)

                                                                                                         ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                                        <  2:1251882/150‑1 (MQ=255)

                                                                                                           tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGGGGGGAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTagaaccgtc                                      >  2:3378943‑M1/1‑141 (MQ=255)

                                                                                                           tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgc                                      >  2:500134/1‑150 (MQ=255)

                                                                                                            gCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCt                                     <  1:2860974/150‑1 (MQ=255)

                                                                                                                    gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGTGTTCAGGGACTTTTGAAAGTGATTGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                             >  1:4058375/1‑150 (MQ=255)

                                                                                                                     ctctctCGCTGCCGCGCGTGTCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGCTCGCGGATGCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                            <  2:2797820/150‑1 (MQ=11)

                                                                                                                     ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                            >  1:1913577/1‑150 (MQ=255)

                                                                                                                       ctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCa                                          >  1:1978032/1‑134 (MQ=255)

                                                                                                                        tctcGCTGCCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGTTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGNGAAGGATTcgaaccttcgaagtcgatgacg                         <  1:3858628‑M1/150‑23 (MQ=255)

                                                                                                                         ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTACCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGGTCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAAGGATTagaaccatcgtagaccatgacgg                        >  1:1007804‑M1/1‑127 (MQ=255)

                                                                                                                                aCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgggg                 >  1:920692/1‑149 (MQ=255)

                                                                                                                                  gCTCCAGCCGAACCTTCGTCGAAGCTTCTCCTCCTTCCCCGCATGCGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGCTGGTGGTGGGGGACGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg               <  1:1982147/150‑1 (MQ=14)

                                                                                                                                   cTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGt              >  2:2344541/1‑150 (MQ=255)

                                                                                                                                   cTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCATCGGGTCGt              >  2:3921523/1‑150 (MQ=255)

                                                                                                                                    tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGtt             <  1:3794460/150‑1 (MQ=255)

                                                                                                                                       aGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc          >  2:3110142/1‑150 (MQ=255)

                                                                                                                                        gCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCGTCGGGTCGTTGCCt         >  2:647682/1‑150 (MQ=255)

                                                                                                                                           gAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCg      >  2:2741359/1‑150 (MQ=255)

                                                                                                                                               cTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCGGGGGCaa  >  1:876361/1‑150 (MQ=255)

AGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAA  >  CP009273/1282293‑1282585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`