Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,520,062 G→T 11.3% A59E (GCG→GAG)  ansP ← L‑asparagine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,520,0620GT11.3% 860.6 / 53.8 242A59E (GCG→GAG) ansPL‑asparagine transporter
Reads supporting (aligned to +/- strand):  ref base G (124/90);  new base T (15/13);  total (139/103)
Fisher's exact test for biased strand distribution p-value = 6.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 246 total aligned reads displayed.

AAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCT  >  CP009273/1519916‑1520211
                                                                                                                                                  |                                                                                                                                                     
aaGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCa                                                                                                                                                    <  2:2909069/150‑1 (MQ=255)
aaGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCa                                                                                                                                                    >  2:2295783/1‑150 (MQ=255)
aaGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCa                                                                                                                                                    >  2:2295837/1‑150 (MQ=255)
   cGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCAttt                                                                                                                                                 >  1:1857277/1‑150 (MQ=255)
   cGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCAttt                                                                                                                                                 <  1:420716/150‑1 (MQ=255)
     gCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGc                                                                                                                                               <  1:3951758/150‑1 (MQ=255)
      cTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCa                                                                                                                                              >  2:3169125/1‑150 (MQ=255)
       tttCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAg                                                                                                                                             >  1:1147330/1‑150 (MQ=255)
        ttCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGt                                                                                                                                            >  2:1482440/1‑150 (MQ=255)
         tctcACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCACTGCCAGTGCGGGCCCCTCCATTTGCAGTc                                                                                                                                           <  1:875162/150‑1 (MQ=255)
          ctcACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCg                                                                                                                                          >  2:3216027/1‑150 (MQ=255)
            cACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCggg                                                                                                                                        >  1:1832200/1‑150 (MQ=255)
                cAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTcc                                                                                                                                    <  2:4149610/150‑1 (MQ=255)
                  aaaaCTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTg                                                                                                                                  <  1:3281101/150‑1 (MQ=255)
                  aaaaCTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTg                                                                                                                                  <  1:2059749/150‑1 (MQ=255)
                   aaaCTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGc                                                                                                                                 <  1:1341899/150‑1 (MQ=255)
                   aaaCTCACGGGCATAAGAAACAAAACTGCCACAGGAAGGGCGGTGTAGCACCAGCTCACCCAAAGCACGCAGAATAAAAAACGAAAAAAAGCCAAAAATTAAATAAACCAGTGCCAGTGCTGGCCCCGCAATTTGCAGTCGGGCTCCTGc                                                                                                                                 >  1:3416716/1‑150 (MQ=255)
                      cTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCAcc                                                                                                                              <  1:1780901/150‑1 (MQ=255)
                      cTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCAcc                                                                                                                              >  1:3155673/1‑150 (MQ=255)
                          cGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTaaa                                                                                                                          >  1:2012283/1‑150 (MQ=255)
                             gCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAAc                                                                                                                       <  2:1726255/150‑1 (MQ=255)
                              cATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACa                                                                                                                      <  2:3979101/150‑1 (MQ=255)
                               aTAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACaa                                                                                                                     >  1:1011071/1‑150 (MQ=255)
                               aTAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACaa                                                                                                                     >  1:3836662/1‑150 (MQ=255)
                                tAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAg                                                                                                                    >  2:374760/1‑150 (MQ=255)
                                tAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAg                                                                                                                    <  2:775481/150‑1 (MQ=255)
                                 aaGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGc                                                                                                                   >  1:314886/1‑150 (MQ=255)
                                  aGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGcc                                                                                                                  <  1:2840672/150‑1 (MQ=255)
                                   gAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCg                                                                                                                 >  2:3311331/1‑150 (MQ=255)
                                     aaCAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGt                                                                                                               <  2:2971548/150‑1 (MQ=255)
                                     aaCAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGt                                                                                                               >  1:2015850/1‑150 (MQ=255)
                                         aaaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCa                                                                                                           <  1:2519438/150‑1 (MQ=255)
                                          aaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCaa                                                                                                          >  1:3900290/1‑150 (MQ=255)
                                          aaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCaa                                                                                                          <  2:1238346/150‑1 (MQ=255)
                                          aaCTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCaa                                                                                                          <  2:488304/150‑1 (MQ=255)
                                            cTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATc                                                                                                        <  2:1239936/150‑1 (MQ=255)
                                             tGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcg                                                                                                       >  2:3020388/1‑150 (MQ=255)
                                               ccACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATcgcg                                                                                                     >  1:1009196/1‑150 (MQ=255)
                                                 aCTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGcgcc                                                                                                   <  2:3115217/150‑1 (MQ=255)
                                                 aCTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGcgcc                                                                                                   <  1:2007342/150‑1 (MQ=255)
                                                   tGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGAGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGcgccgc                                                                                                 >  2:2515480/1‑150 (MQ=255)
                                                     gAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCa                                                                                               <  2:2487830/150‑1 (MQ=255)
                                                     gAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCa                                                                                               >  1:706997/1‑150 (MQ=255)
                                                      aaGGGCGGTGTAGCACCAGATCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCaa                                                                                              <  2:2459649/150‑1 (MQ=255)
                                                            ggTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGa                                                                                        >  2:2715035/1‑150 (MQ=255)
                                                            ggTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGa                                                                                        >  1:2957274/1‑150 (MQ=255)
                                                              tgtAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAACTAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGatc                                                                                      <  1:4103639/150‑1 (MQ=255)
                                                                   cACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                                                                 >  1:2936930/1‑150 (MQ=255)
                                                                   cACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                                                                 >  1:2194837/1‑150 (MQ=255)
                                                                   cACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTg                                                                                 >  1:2936829/1‑150 (MQ=255)
                                                                    aCCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGc                                                                                <  2:925881/150‑1 (MQ=255)
                                                                    aCCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGc                                                                                >  1:839883/1‑150 (MQ=255)
                                                                      cAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCAc                                                                              >  2:1412030/1‑150 (MQ=255)
                                                                       aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCAcc                                                                             >  1:2686806/1‑150 (MQ=255)
                                                                       aGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCAcc                                                                             >  1:468980/1‑150 (MQ=255)
                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCt                                                                            <  2:2065243/150‑1 (MQ=255)
                                                                        gCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCACCAATGGCGATCATCTGCACCt                                                                            >  1:2173291/1‑150 (MQ=255)
                                                                         cTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTg                                                                           >  2:2691919/1‑150 (MQ=255)
                                                                         cTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTg                                                                           <  1:1798870/150‑1 (MQ=255)
                                                                              ccAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGAt                                                                      <  2:2141071/150‑1 (MQ=255)
                                                                                aaTGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTg                                                                    <  1:1501795/150‑1 (MQ=255)
                                                                                   gCACGCAGAATAAAAAACGAACACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGcct                                                                 <  2:3999112/150‑2 (MQ=255)
                                                                                     aCGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCAt                                                               >  1:934357/1‑150 (MQ=255)
                                                                                         aGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCCTTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                           >  2:415496/1‑150 (MQ=255)
                                                                                         aGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACACGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                           >  2:810177/1‑150 (MQ=255)
                                                                                         aGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                           >  1:2319627/1‑150 (MQ=255)
                                                                                         aGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCt                                                           >  1:2852796/1‑150 (MQ=255)
                                                                                          gAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCtt                                                          >  2:74641/1‑150 (MQ=255)
                                                                                            aTAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtg                                                        >  2:3868682/1‑150 (MQ=255)
                                                                                              aaaaaaCGAAAACCAGCCACAAATTACATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtgtg                                                      <  2:3776725/150‑1 (MQ=255)
                                                                                              aaaaaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTtgtg                                                      >  1:1641380/1‑150 (MQ=255)
                                                                                                 aaaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATa                                                   <  2:2433481/150‑1 (MQ=255)
                                                                                                  aaCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAc                                                  >  1:1021620/1‑150 (MQ=255)
                                                                                                   aCGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTAATAcc                                                 <  2:2551233/150‑1 (MQ=255)
                                                                                                     gAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATAcccc                                               <  2:338897/150‑1 (MQ=255)
                                                                                                       aaaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCctc                                             >  1:4024574/1‑150 (MQ=255)
                                                                                                       aaaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCctc                                             <  1:1452740/150‑1 (MQ=255)
                                                                                                        aaCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCctct                                            >  1:3203408/1‑150 (MQ=255)
                                                                                                         aCAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTAATACCCCTCtt                                           <  1:654170/150‑1 (MQ=255)
                                                                                                          cAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTc                                          <  1:1891712/150‑1 (MQ=255)
                                                                                                           aaGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCg                                         <  2:2644004/150‑1 (MQ=255)
                                                                                                            aGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGt                                        >  1:3656238/1‑150 (MQ=255)
                                                                                                             gCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTg                                       <  2:1885027/150‑1 (MQ=255)
                                                                                                              ccACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTgg                                      >  1:169853/1‑150 (MQ=255)
                                                                                                              ccACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTgg                                      >  1:152107/1‑150 (MQ=255)
                                                                                                                   aaTTAAATAAACCAGTGCCAGTGCGGGCCCCTCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCAtt                                 <  2:2213497/150‑1 (MQ=255)
                                                                                                                         ataaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCa                           <  2:3150064/150‑1 (MQ=255)
                                                                                                                          taaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAg                          >  1:1420964/1‑150 (MQ=255)
                                                                                                                           aaaCCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAgc                         >  2:1130297/1‑150 (MQ=255)
                                                                                                                                 gtgccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGtt                   >  2:3469432/1‑150 (MQ=255)
                                                                                                                                   gccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTc                 <  2:3415821/150‑1 (MQ=255)
                                                                                                                                   gccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTAATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTc                 <  1:3065944/150‑1 (MQ=255)
                                                                                                                                    ccagtgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTcg                >  2:3812144/1‑150 (MQ=255)
                                                                                                                                        tgcGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATCGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCgcggc            <  2:1655779/150‑1 (MQ=255)
                                                                                                                                           gggCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCgtg         >  2:3955613/1‑150 (MQ=255)
                                                                                                                                              ccccGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTg      >  2:1585602/1‑150 (MQ=255)
                                                                                                                                               cccGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGa     <  1:300226/150‑1 (MQ=255)
                                                                                                                                                 cGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATc   <  1:4097736/150‑1 (MQ=255)
                                                                                                                                                  gCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCt  >  2:549385/1‑150 (MQ=255)
                                                                                                                                                  gCCATGTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCt  <  2:1045804/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AAGCGGCTTTCTCACCCAAAAACTCACGGGCATAAGAAACAAAACTGCCACTGGAAGGGCGGTGTAGCACCAGCTCACCCAATGCACGCAGAATAAAAAACGAAAACAAGCCACAAATTAAATAAACCAGTGCCAGTGCGGGCCCCGCCATTTGCAGTCGGGCTCCTGCACCTAAAAACAAGCCGGTGCCAATCGCGCCGCCAATGGCGATCATCTGCACCTGGCGATTGCCCATCGCTTTGTGATACCCCTCTTCGTGGGCATTAAGCCAGCGGCGTTTCGCGGCGTGTTGATCT  >  CP009273/1519916‑1520211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑