Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,529,506 C→T 10.4% G204S (GGC→AGC)  yddE ← PhzC‑PhzF family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,529,5060CT10.4% 997.8 / 76.2 306G204S (GGC→AGC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base C (115/159);  new base T (14/18);  total (129/177)
Fisher's exact test for biased strand distribution p-value = 8.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTG  >  CP009273/1529357‑1529653
                                                                                                                                                     |                                                                                                                                                   
gCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGcc                                                                                                                                                     <  2:1130512/150‑1 (MQ=255)
  cTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTg                                                                                                                                                   >  1:2934083/1‑150 (MQ=255)
   tttAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTgg                                                                                                                                                  <  2:881700/150‑1 (MQ=255)
   tttAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTgg                                                                                                                                                  <  2:2639957/150‑1 (MQ=255)
     tAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGc                                                                                                                                                >  2:3246338/1‑150 (MQ=255)
      aaCACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCg                                                                                                                                               <  1:892536/150‑1 (MQ=255)
           gCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCt                                                                                                                                          <  2:815885/150‑1 (MQ=255)
            cAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTg                                                                                                                                         <  1:2788407/150‑1 (MQ=255)
                acGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAAc                                                                                                                                     <  1:1520103/150‑1 (MQ=255)
                   ttGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACggg                                                                                                                                  <  2:1359022/150‑1 (MQ=255)
                   ttGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACggg                                                                                                                                  <  2:3538031/150‑1 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                 <  2:2738390/150‑1 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                 >  1:1000456/1‑150 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                 <  2:3700335/150‑1 (MQ=255)
                    tGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGga                                                                                                                                 <  2:1722241/150‑1 (MQ=255)
                      ccATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGgaag                                                                                                                               >  1:1874334/1‑150 (MQ=255)
                       cATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGgaaga                                                                                                                              >  1:2061428/1‑150 (MQ=255)
                           gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                          >  1:1924008/1‑150 (MQ=255)
                           gTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAAcc                                                                                                                          >  2:3482489/1‑150 (MQ=255)
                             ggggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGTCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCAt                                                                                                                        >  2:1329537/1‑150 (MQ=255)
                               ggCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTg                                                                                                                      <  2:1851009/150‑1 (MQ=255)
                                gCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGc                                                                                                                     <  2:1749925/150‑1 (MQ=255)
                                 cAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCa                                                                                                                    <  1:3964207/150‑1 (MQ=255)
                                  aaTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAg                                                                                                                   >  1:1807881/1‑150 (MQ=255)
                                  aaTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAg                                                                                                                   <  2:1177037/150‑1 (MQ=255)
                                  aaTACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAg                                                                                                                   >  1:1212159/1‑150 (MQ=255)
                                     aCGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCa                                                                                                                <  2:2265964/150‑1 (MQ=255)
                                     aCGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCa                                                                                                                >  2:2736054/1‑150 (MQ=255)
                                       gTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAAt                                                                                                              <  1:1687155/150‑1 (MQ=255)
                                        ttGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCGGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATc                                                                                                             >  2:1554495/1‑150 (MQ=255)
                                         tgtgATGTACCACCCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCt                                                                                                            <  1:3894485/150‑1 (MQ=255)
                                         tgtgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCt                                                                                                            >  2:2311804/1‑150 (MQ=255)
                                           tgATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttt                                                                                                          >  2:3107181/1‑150 (MQ=255)
                                             aTGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCttttt                                                                                                        <  2:1068823/150‑1 (MQ=255)
                                                tACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCt                                                                                                     <  2:1281283/150‑1 (MQ=255)
                                                  ccaaccaTGCACCCCTCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGa                                                                                                   <  2:3469040/150‑1 (MQ=255)
                                                       caTGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCg                                                                                              <  2:1643020/150‑1 (MQ=255)
                                                         tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGt                                                                                            >  2:1453492/1‑150 (MQ=255)
                                                         tGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGt                                                                                            >  1:1831328/1‑150 (MQ=255)
                                                           cACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCa                                                                                          >  2:3757576/1‑150 (MQ=255)
                                                             cccATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgc                                                                                        >  2:2191546/1‑150 (MQ=255)
                                                             cccATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTCTTGCTGATAGCGGTCAgc                                                                                        <  1:4066090/150‑1 (MQ=255)
                                                              ccATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAgcg                                                                                       >  2:2491467/1‑150 (MQ=255)
                                                                aTCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCa                                                                                     <  1:638823/150‑1 (MQ=255)
                                                                  cGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCAtt                                                                                   >  1:4168498/1‑150 (MQ=255)
                                                                    ggCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTaa                                                                                 >  1:1953588/1‑150 (MQ=255)
                                                                     gCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAg                                                                                <  2:2520016/150‑1 (MQ=255)
                                                                     gCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAg                                                                                <  1:593070/150‑1 (MQ=255)
                                                                       ctttGGCATTGCAAGTCACCGGATCTTCCACTATACAAATCGCAGGCGATTACATGCTACCATCGGTTTCTTTTTTGCTTGGTCGGATCTTTAACGGTAAGATACCATTGCAGCCAATCTTTTTGCTGATATCGGTCAGCGCATTAAGGt                                                                              <  2:2448930/148‑1 (MQ=255)
                                                                       cATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGt                                                                              <  2:3587569/150‑1 (MQ=255)
                                                                         ttGGCATTGCCAGTCACCGGATCTTCCACAATCCCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCa                                                                            <  2:3782521/150‑1 (MQ=255)
                                                                           ggCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAgg                                                                          <  2:406680/150‑1 (MQ=255)
                                                                           ggCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAgg                                                                          <  1:3539546/150‑1 (MQ=255)
                                                                             cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                        <  2:1673452/150‑1 (MQ=255)
                                                                             cATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCg                                                                        <  1:3420778/150‑1 (MQ=255)
                                                                                tGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGaaa                                                                     <  1:3511556/150‑1 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                    >  1:3150969/1‑150 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                    >  2:3997726/1‑150 (MQ=255)
                                                                                 gCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAg                                                                    >  1:2662358/1‑150 (MQ=255)
                                                                                    aGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGc                                                                 <  1:3950254/150‑1 (MQ=255)
                                                                                       cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                              >  1:2145143/1‑150 (MQ=255)
                                                                                       cACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTc                                                              <  1:3184423/150‑1 (MQ=255)
                                                                                           ggATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGata                                                          <  2:2585147/150‑1 (MQ=255)
                                                                                            gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                         >  1:1636558/1‑150 (MQ=255)
                                                                                            gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                         >  2:1244233/1‑150 (MQ=255)
                                                                                            gATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGatat                                                         >  2:4143270/1‑150 (MQ=255)
                                                                                               cTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCa                                                      <  1:3339003/150‑1 (MQ=255)
                                                                                                ttCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCAc                                                     >  1:1988057/1‑150 (MQ=255)
                                                                                                   cacaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTc                                                  <  2:3805261/150‑1 (MQ=255)
                                                                                                   cacaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTc                                                  >  2:2742679/1‑150 (MQ=255)
                                                                                                    acaATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTcc                                                 <  2:677070/150‑1 (MQ=255)
                                                                                                     caATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCg                                                >  1:2387880/1‑150 (MQ=255)
                                                                                                     caATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCg                                                >  2:4132360/1‑150 (MQ=255)
                                                                                                       aTACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGt                                              <  1:2463293/150‑1 (MQ=255)
                                                                                                             aTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGTTCTGTAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGATAGGGCGTCGATATCCACTTCCGGTTTCAGt                                        <  1:1272400/150‑1 (MQ=255)
                                                                                                             aTCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGt                                        >  1:1542529/1‑150 (MQ=255)
                                                                                                                 cAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGa                                    <  1:3003891/150‑1 (MQ=255)
                                                                                                                     cGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcat                                <  2:306558/150‑1 (MQ=255)
                                                                                                                     cGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcat                                <  2:3476505/150‑1 (MQ=255)
                                                                                                                      gagaACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGatcatc                               <  1:1627014/150‑1 (MQ=255)
                                                                                                                         aaCATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACt                            >  1:774519/1‑150 (MQ=255)
                                                                                                                           cATGCGACCATCGGTTTCGTTTTTGCTTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACttt                          <  2:2793899/150‑1 (MQ=255)
                                                                                                                           cATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCAGTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACttt                          <  2:1563387/150‑1 (MQ=255)
                                                                                                                               cGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAg                      <  1:3762904/150‑1 (MQ=255)
                                                                                                                                gACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgt                     >  1:2102359/1‑150 (MQ=255)
                                                                                                                                 aCCATCGGTTTCGTTTTTGCTTGGGCGGATNTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTACGGTTTCAGTGGGATCATCACTGTTGAgtg                    >  1:3858735/1‑150 (MQ=255)
                                                                                                                                  ccATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAgtgt                   >  1:27260/1‑150 (MQ=255)
                                                                                                                                   cATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTc                  <  2:3863242/150‑1 (MQ=255)
                                                                                                                                    aTCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTcc                 <  1:2562528/150‑1 (MQ=255)
                                                                                                                                     tCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCt                <  2:3256562/150‑1 (MQ=255)
                                                                                                                                      cGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtg               <  2:2341713/150‑1 (MQ=255)
                                                                                                                                       ggTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgt              >  2:1968510/1‑150 (MQ=255)
                                                                                                                                          ttCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCtgttgt           >  2:2629105/1‑150 (MQ=255)
                                                                                                                                           tCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTgttgtt          <  1:1352968/150‑1 (MQ=255)
                                                                                                                                            cGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGttgttg         <  1:1133150/150‑1 (MQ=255)
                                                                                                                                                 ttGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCAcc    <  2:2910439/150‑1 (MQ=255)
                                                                                                                                                  tGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCt   <  2:2626430/150‑1 (MQ=255)
                                                                                                                                                  tGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCt   <  1:3305894/150‑1 (MQ=255)
                                                                                                                                                  tGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCt   <  2:502760/150‑1 (MQ=255)
                                                                                                                                                   gCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTg  <  1:607079/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GCCTTTAACACGCAACACGTTGCCATCGTGGGGCAATACGTTGTGATGTACCAACCATGCACCCATCGGGCCATTGGCATTGCCAGTCACCGGATCTTCCACAATACCAATCGCAGGCGAGAACATGCGACCATCGGTTTCGTTTTTGCCTGGGCGGATCTGGAACGGGAAGAAACCATTGCAGCCAATCTTTTTGCTGATAGCGGTCAGCGCATTAAGGTCAGGCGAAAGGGCGTCGATATCCACTTCCGGTTTCAGTGGGATCATCACTTTTGAGTGTCCTGTTGTTGCCACCTG  >  CP009273/1529357‑1529653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑