Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,602,131 G→T 44.0% D177Y (GAT→TAT)  tam → trans‑aconitate methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,602,1310GT44.0% 208.8 / 297.4 232D177Y (GAT→TAT) tamtrans‑aconitate methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (56/74);  new base T (54/48);  total (110/122)
Fisher's exact test for biased strand distribution p-value = 1.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 237 total aligned reads displayed.

CAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGA  >  CP009273/1601982‑1602279
                                                                                                                                                     |                                                                                                                                                    
cAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCg                                                                                                                                                      >  2:461088/1‑150 (MQ=255)
cAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCg                                                                                                                                                      <  1:1344846/150‑1 (MQ=255)
  gTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGat                                                                                                                                                    <  1:2268820/150‑1 (MQ=255)
    aCAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTatat                                                                                                                                                  <  2:1330973/150‑1 (MQ=255)
      aGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCt                                                                                                                                                >  1:965478/1‑150 (MQ=255)
       gATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTg                                                                                                                                               <  1:2400796/150‑1 (MQ=255)
          gCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCg                                                                                                                                            >  2:2219192/1‑150 (MQ=255)
              gATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAacc                                                                                                                                        <  1:17280/150‑1 (MQ=255)
              gATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAacc                                                                                                                                        <  1:17828/150‑1 (MQ=255)
                   ctggctggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACcta                                                                                                                                   <  2:3935595/150‑1 (MQ=255)
                    tggctggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACctac                                                                                                                                  >  1:1486472/1‑150 (MQ=255)
                        tggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATc                                                                                                                              >  1:3914725/1‑150 (MQ=255)
                        tggAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATc                                                                                                                              >  1:1848186/1‑150 (MQ=255)
                              cGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGa                                                                                                                        >  1:3340343/1‑150 (MQ=255)
                               gACCCATGTGCTCATGCGCGAAGTTGCCTGGGACCAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGAt                                                                                                                       >  2:1179409/1‑150 (MQ=255)
                               gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGAt                                                                                                                       >  2:2254426/1‑150 (MQ=255)
                               gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGAt                                                                                                                       >  2:2798159/1‑150 (MQ=255)
                               gACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGAt                                                                                                                       >  2:3652361/1‑150 (MQ=255)
                                aCCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATg                                                                                                                      <  2:793425/150‑1 (MQ=255)
                                aCCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATg                                                                                                                      <  2:2177681/150‑1 (MQ=255)
                                aCCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATg                                                                                                                      >  1:1185239/1‑150 (MQ=255)
                                 cccATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGc                                                                                                                     >  1:343296/1‑150 (MQ=255)
                                  ccATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGcc                                                                                                                    <  2:1496347/150‑1 (MQ=255)
                                    aTGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGt                                                                                                                  <  2:255018/150‑1 (MQ=255)
                                    aTGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGt                                                                                                                  <  2:255248/150‑1 (MQ=255)
                                            aTGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAg                                                                                                          >  2:1118022/1‑150 (MQ=255)
                                             tGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAgg                                                                                                         >  1:1832394/1‑150 (MQ=255)
                                                gcgAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGa                                                                                                      <  1:2547023/150‑1 (MQ=255)
                                                gcgAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGa                                                                                                      <  2:1404860/150‑1 (MQ=255)
                                                  gAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGAtt                                                                                                    >  1:438203/1‑150 (MQ=255)
                                                   aaGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTa                                                                                                   >  2:4138649/1‑150 (MQ=255)
                                                   aaGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTa                                                                                                   >  1:2575443/1‑150 (MQ=255)
                                                    aGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTAt                                                                                                  >  2:965383/1‑150 (MQ=255)
                                                       tGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGa                                                                                               >  2:3984546/1‑150 (MQ=255)
                                                        gCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGAt                                                                                              >  2:2354340/1‑150 (MQ=255)
                                                         ccTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGAtt                                                                                             >  1:233175/1‑150 (MQ=255)
                                                         ccTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGAtt                                                                                             <  2:4032248/150‑1 (MQ=255)
                                                         ccTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGAtt                                                                                             <  1:2955801/150‑1 (MQ=255)
                                                          cTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTg                                                                                            >  1:661476/1‑150 (MQ=255)
                                                           tGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTgg                                                                                           >  2:1827614/1‑150 (MQ=255)
                                                           tGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTgg                                                                                           >  2:2995253/1‑150 (MQ=255)
                                                             ggAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGt                                                                                         >  2:2187980/1‑150 (MQ=255)
                                                             ggAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGt                                                                                         >  1:3310691/1‑150 (MQ=255)
                                                                  aaaaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTg                                                                                    <  2:1208149/150‑1 (MQ=255)
                                                                   aaaCTACCCGGATCGCGGGCGTGAGCAGTTGGCTGTAGTTCATGCTTACTACGATATTTTGATAGAAGCCGGATGTGAGGTAGATATATGGCGAACCACCTACTATCAACAGAATCCCACACACAATGCGATTATCGATTGGGTGAATGc                                                                                   >  1:2177639/1‑150 (MQ=255)
                                                                    aaCTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGcc                                                                                  >  1:2006017/1‑150 (MQ=255)
                                                                       tACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACt                                                                               <  2:2895172/150‑1 (MQ=255)
                                                                        aCCCGGATCGCGGGCGTGAGCCGTTGGCTGTCGTTCATGCTTACTACGATATATTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTg                                                                              <  2:3534538/150‑1 (MQ=255)
                                                                           cGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGAt                                                                           >  1:3209169/1‑150 (MQ=255)
                                                                           cGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGAt                                                                           >  1:1261780/1‑150 (MQ=255)
                                                                            ggATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGAtt                                                                          >  2:2101800/1‑150 (MQ=255)
                                                                            ggATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGAtt                                                                          <  1:831135/150‑1 (MQ=255)
                                                                              aTCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTAc                                                                        <  1:3271214/150‑1 (MQ=255)
                                                                               tCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACg                                                                       >  1:2165847/1‑150 (MQ=255)
                                                                                cgcgGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGt                                                                      >  2:2613084/1‑150 (MQ=255)
                                                                                  cgGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTcc                                                                    <  2:3853961/150‑1 (MQ=255)
                                                                                  cgGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTcc                                                                    <  2:1795987/150‑1 (MQ=255)
                                                                                   gggCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCg                                                                   >  2:3012854/1‑150 (MQ=255)
                                                                                        tGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGtt                                                              >  2:367699/1‑150 (MQ=255)
                                                                                         gAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTa                                                             >  1:3451246/1‑150 (MQ=255)
                                                                                             cGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAgg                                                         <  2:2115771/150‑1 (MQ=255)
                                                                                               ttGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGAt                                                       <  1:1452394/150‑1 (MQ=255)
                                                                                                 ggctggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCt                                                     <  1:1123375/150‑1 (MQ=255)
                                                                                                 ggctggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCt                                                     <  1:2034634/150‑1 (MQ=255)
                                                                                                  gctggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTg                                                    <  1:3115478/150‑1 (MQ=255)
                                                                                                  gctggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTg                                                    <  1:2906848/150‑1 (MQ=255)
                                                                                                    tggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGAc                                                  >  1:2197768/1‑150 (MQ=255)
                                                                                                     ggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGAcc                                                 <  2:1708015/150‑1 (MQ=255)
                                                                                                     ggcGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGAcc                                                 <  2:677634/150‑1 (MQ=255)
                                                                                                       cGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCga                                               >  2:1117407/1‑150 (MQ=255)
                                                                                                        gTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCgag                                              >  2:432452/1‑150 (MQ=255)
                                                                                                         ttCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGNTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTNCAGGATCTGACCgagn                                             <  2:284694/150‑2 (MQ=255)
                                                                                                              gCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTTTATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGTTTGGGTGACTGCCACTGGATTTCGTCCGTGGTTACAGGATCTGACCGAGAGCGaa                                        <  2:3380337/150‑1 (MQ=255)
                                                                                                              gCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGaa                                        <  2:3380637/150‑1 (MQ=255)
                                                                                                               cTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAc                                       >  2:3716249/1‑150 (MQ=255)
                                                                                                               cTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAc                                       <  1:4098992/150‑1 (MQ=255)
                                                                                                                   ctacGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAcagca                                   <  2:659522/150‑1 (MQ=255)
                                                                                                                   ctacGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAcagca                                   <  1:3580121/150‑1 (MQ=255)
                                                                                                                   ctacGATATATTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAcagca                                   <  1:3177420/150‑1 (MQ=255)
                                                                                                                    tacGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAAcagcag                                  >  1:1047453/1‑150 (MQ=255)
                                                                                                                     acGATATTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACagcagc                                 <  2:3270021/150‑1 (MQ=255)
                                                                                                                     acGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACagcagc                                 <  1:1586033/150‑1 (MQ=255)
                                                                                                                        atatTTTGAGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCttt                              <  2:3149663/150‑1 (MQ=255)
                                                                                                                            tttGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCt                          <  2:1976129/150‑1 (MQ=255)
                                                                                                                            tttGAGCGAAGCCGGATGTGAGGTAGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACAACAGGCGATTATCGATTGGGTGACTGCAACTGGATTACGTACGTGGTTAGAGGTTCTGACCGAGAGCTAACAGCATCtttttat                          <  2:917576/150‑3 (MQ=255)
                                                                                                                             ttGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCtt                         <  2:747444/150‑1 (MQ=255)
                                                                                                                                aGCGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAg                      <  1:1118990/150‑1 (MQ=255)
                                                                                                                                  cGAAGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAgcg                    <  1:3630123/150‑1 (MQ=255)
                                                                                                                                  cGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAgcg                    <  2:2699827/150‑1 (MQ=255)
                                                                                                                                  cGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAgcg                    >  1:672691/1‑150 (MQ=255)
                                                                                                                                  cGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAgcg                    <  2:779163/150‑1 (MQ=255)
                                                                                                                                    aaGCCGGATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCt                  <  2:1577165/150‑1 (MQ=255)
                                                                                                                                    aaGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCt                  >  1:1405443/1‑150 (MQ=255)
                                                                                                                                     aGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTa                 <  1:3700917/150‑1 (MQ=255)
                                                                                                                                         ggATGTGAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCAt             >  1:2769456/1‑150 (MQ=255)
                                                                                                                                         ggATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCAt             >  1:2858246/1‑150 (MQ=255)
                                                                                                                                         ggATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCAt             <  2:522257/150‑1 (MQ=255)
                                                                                                                                               gAGGTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATg       >  1:1902167/1‑150 (MQ=255)
                                                                                                                                                  gTCTATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTg    <  1:2851338/150‑1 (MQ=255)
                                                                                                                                                  gTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTg    >  1:849792/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGTACAGATGCCAGATAACTGGCTGGAGCCGACCCATGTGCTCATGCGCGAAGTTGCCTGGGAACAAAACTACCCGGATCGCGGGCGTGAGCCGTTGGCTGGCGTTCATGCTTACTACGATATTTTGAGCGAAGCCGGATGTGAGGTCGATATCTGGCGAACCACCTACTATCACCAGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGA  >  CP009273/1601982‑1602279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑