Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,689,180 T→G 56.4% E383D (GAA→GAC)  uidA ← beta‑D‑glucuronidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,689,1800TG56.4% 207.0 / 243.9 203E383D (GAA→GAC) uidAbeta‑D‑glucuronidase
Reads supporting (aligned to +/- strand):  ref base T (42/47);  new base G (40/74);  total (82/121)
Fisher's exact test for biased strand distribution p-value = 8.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 204 total aligned reads displayed.

CGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTT  >  CP009273/1689031‑1689325
                                                                                                                                                     |                                                                                                                                                 
cgcgTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGtt                                                                                                                                                   <  1:1362817/150‑1 (MQ=255)
  cgTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTc                                                                                                                                                 >  2:1305624/1‑150 (MQ=255)
  cgTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTc                                                                                                                                                 >  2:3398734/1‑150 (MQ=255)
   gTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTcc                                                                                                                                                >  1:196560/1‑150 (MQ=255)
     tGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTcccc                                                                                                                                              >  1:1427287/1‑150 (MQ=255)
      gCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCg                                                                                                                                             <  1:2361521/150‑1 (MQ=255)
         tCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTg                                                                                                                                          >  2:3336371/1‑150 (MQ=255)
         tCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTg                                                                                                                                          <  1:2416737/150‑1 (MQ=255)
            gCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACt                                                                                                                                       >  1:913903/1‑150 (MQ=255)
            gCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACt                                                                                                                                       >  1:3344004/1‑150 (MQ=255)
            gCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACt                                                                                                                                       <  2:3307504/150‑1 (MQ=255)
             ccAGTGGCGCGAAATAGTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTg                                                                                                                                      <  2:1712426/150‑1 (MQ=255)
              cAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGc                                                                                                                                     <  1:295451/150‑1 (MQ=255)
               aGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGcc                                                                                                                                    >  2:2629244/1‑150 (MQ=255)
               aGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGcc                                                                                                                                    >  1:2521484/1‑150 (MQ=255)
                gTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTTGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCct                                                                                                                                   <  1:2076887/150‑1 (MQ=255)
                 tGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCctc                                                                                                                                  >  2:3221998/1‑150 (MQ=255)
                 tGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCctc                                                                                                                                  <  2:3721582/150‑1 (MQ=255)
                   gcgcGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCtt                                                                                                                                <  1:54027/150‑1 (MQ=255)
                    cgcgAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTc                                                                                                                               >  1:1595718/1‑150 (MQ=255)
                       gAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCt                                                                                                                            >  2:1345943/1‑150 (MQ=255)
                        aaaTATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTg                                                                                                                           >  1:2718853/1‑150 (MQ=255)
                          atatTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTa                                                                                                                         >  2:1221349/1‑150 (MQ=255)
                           tatTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTAc                                                                                                                        >  1:3638958/1‑150 (MQ=255)
                            atTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                       <  2:1362785/150‑1 (MQ=255)
                            atTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                       >  2:3520254/1‑150 (MQ=255)
                            agtCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACa                                                                                                                       <  1:4056094/148‑1 (MQ=255)
                               cccGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGtt                                                                                                                    >  2:3423100/1‑150 (MQ=255)
                               cccGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGtt                                                                                                                    <  1:212947/150‑1 (MQ=255)
                                   tGCACCGTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCACGTTGACTGCCTCTTCGCTGTACAGTTCttt                                                                                                                <  2:3224705/150‑1 (MQ=255)
                                    gCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTc                                                                                                               <  1:3318290/150‑1 (MQ=255)
                                    gCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTc                                                                                                               <  1:2747810/150‑1 (MQ=255)
                                    gCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTc                                                                                                               <  2:1183505/150‑1 (MQ=255)
                                         ttGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCtt                                                                                                          <  2:3629151/150‑1 (MQ=255)
                                          tGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCttg                                                                                                         >  1:2359580/1‑150 (MQ=255)
                                                   gTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCt                                                                                                <  1:3390563/150‑1 (MQ=255)
                                                   gTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCt                                                                                                <  2:2397051/150‑1 (MQ=255)
                                                    tATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCtt                                                                                               <  2:2593175/150‑1 (MQ=255)
                                                      tCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCg                                                                                             <  1:1623578/150‑1 (MQ=255)
                                                         ggTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGaaa                                                                                          <  1:534255/150‑1 (MQ=255)
                                                              gTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAAt                                                                                     <  2:2231303/150‑1 (MQ=255)
                                                              gTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAAt                                                                                     <  1:1521399/150‑1 (MQ=255)
                                                              gTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAAt                                                                                     <  1:1952021/150‑1 (MQ=255)
                                                               ttGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATg                                                                                    <  1:1040048/150‑1 (MQ=255)
                                                                 ggCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGcc                                                                                  <  1:3549039/150‑1 (MQ=255)
                                                                   cAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTa                                                                                <  2:204703/150‑1 (MQ=255)
                                                                    aaTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaa                                                                               <  1:827992/150‑1 (MQ=255)
                                                                    aaTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaa                                                                               <  1:245945/150‑1 (MQ=255)
                                                                     aTACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTaaa                                                                              <  2:2583568/150‑1 (MQ=255)
                                                                      tACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAag                                                                             <  2:10000/150‑1 (MQ=255)
                                                                         tCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                          >  1:2592011/1‑150 (MQ=255)
                                                                         tCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAagaga                                                                          >  1:3521498/1‑150 (MQ=255)
                                                                              aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTa                                                                     >  1:214639/1‑150 (MQ=255)
                                                                              aTCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTa                                                                     <  2:2845693/150‑1 (MQ=255)
                                                                                  ccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGc                                                                 <  2:513744/150‑1 (MQ=255)
                                                                                  ccacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGc                                                                 <  2:37804/150‑1 (MQ=255)
                                                                                   cacGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGcc                                                                >  2:3911549/1‑150 (MQ=255)
                                                                                    acGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCg                                                               >  2:1447905/1‑150 (MQ=255)
                                                                                       cTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAca                                                            <  2:4075048/150‑1 (MQ=255)
                                                                                       cTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAca                                                            <  2:1974944/150‑1 (MQ=255)
                                                                                         tGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                          >  1:109219/1‑150 (MQ=255)
                                                                                         tGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGAcagc                                                          >  1:2265004/1‑150 (MQ=255)
                                                                                           ggtggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCATGTAAGTGCGCTTGCTGAGTTTACCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCATAAAGAGATGTTAAAGCCGAcagcag                                                        >  1:3688448/1‑150 (MQ=255)
                                                                                             tggtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATACCTAAAGAGAGGTTAAAGCCGAcagcagca                                                      >  2:2129771/1‑150 (MQ=255)
                                                                                               gtTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGt                                                    >  2:714964/1‑150 (MQ=255)
                                                                                                  tttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtc                                                 <  2:2616681/150‑1 (MQ=255)
                                                                                                   ttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtca                                                <  1:4033107/150‑1 (MQ=255)
                                                                                                   ttGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtca                                                <  2:1076297/150‑1 (MQ=255)
                                                                                                     gTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtcatc                                              <  2:2744487/150‑1 (MQ=255)
                                                                                                     gTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTtcatc                                              <  1:2083181/150‑1 (MQ=255)
                                                                                                        aCGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaat                                           <  2:1697804/150‑1 (MQ=255)
                                                                                                        aCGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCatcaat                                           <  2:1904967/150‑1 (MQ=255)
                                                                                                           cgcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcac                                        <  2:251663/150‑1 (MQ=255)
                                                                                                           cgcTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcac                                        >  1:4125303/1‑150 (MQ=255)
                                                                                                              tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                     <  2:3738022/150‑1 (MQ=255)
                                                                                                              tATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATcaccac                                     >  2:60177/1‑150 (MQ=255)
                                                                                                                   gCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGc                                >  1:1547628/1‑150 (MQ=255)
                                                                                                                    ctctTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGcc                               >  1:3952579/1‑150 (MQ=255)
                                                                                                                     tctTTAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCa                              >  2:2366717/1‑150 (MQ=255)
                                                                                                                         tAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGtt                          <  2:847046/150‑1 (MQ=255)
                                                                                                                         tAATCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGtt                          <  2:846969/150‑1 (MQ=255)
                                                                                                                          aaTCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTc                         <  2:3039300/150‑1 (MQ=255)
                                                                                                                          aaTCGCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTc                         <  1:2802666/150‑1 (MQ=255)
                                                                                                                           aTCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCa                        <  2:1646988/150‑1 (MQ=255)
                                                                                                                            tCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCTAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCAt                       <  1:420885/150‑1 (MQ=255)
                                                                                                                             cGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATc                      <  1:1305624/150‑1 (MQ=255)
                                                                                                                             cGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATc                      <  1:3398734/150‑1 (MQ=255)
                                                                                                                              gCCTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCt                     >  1:832658/1‑150 (MQ=255)
                                                                                                                               ccTGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTg                    >  2:380915/1‑150 (MQ=255)
                                                                                                                                 tGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGcc                  <  1:3117636/150‑1 (MQ=255)
                                                                                                                                 tGTAAGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGcc                  <  1:458749/150‑1 (MQ=255)
                                                                                                                                     aGTGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGt              <  1:1719380/150‑1 (MQ=255)
                                                                                                                                       tGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCg            <  1:1173204/150‑1 (MQ=255)
                                                                                                                                       tGCGCTTGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCg            <  2:2529679/150‑1 (MQ=255)
                                                                                                                                        gcgcTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGa           >  1:90985/1‑150 (MQ=255)
                                                                                                                                         cgcTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAg          <  2:1517444/150‑1 (MQ=255)
                                                                                                                                            ttGCTGAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAt       <  2:3638960/150‑1 (MQ=255)
                                                                                                                                             tGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCAtc      >  1:651422/1‑150 (MQ=255)
                                                                                                                                                tGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATctct   <  2:3442807/150‑1 (MQ=255)
                                                                                                                                                 gAGTGTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCtt  <  2:668150/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
CGCGTTGCTTCCGCCAGTGGCGCGAAATATTCCCGTGCACCTTGCGGACGGGTATCCGGTTCGTTGGCAATACTCCACATCACCACGCTTGGGTGGTTTTTGTCACGCGCTATCAGCTCTTTAATCGCCTGTAAGTGCGCTTGCTGAGTTTCCCCGTTGACTGCCTCTTCGCTGTACAGTTCTTTCGGCTTGTTGCCCGCTTCGAAACCAATGCCTAAAGAGAGGTTAAAGCCGACAGCAGCAGTTTCATCAATCACCACGATGCCATGTTCATCTGCCCAGTCGAGCATCTCTT  >  CP009273/1689031‑1689325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑