BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,698,343	C→A	41.2%	D75Y (GAT→TAT)	ydgJ ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,698,343	0	C	A	41.2%	255.9 / 269.0	232	D75Y (GAT→TAT)	ydgJ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base C (44/92); new base A (55/41); total (99/133)
Fisher's exact test for biased strand distribution p-value = 2.54e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

ACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTGCCCGGGCGTGCCCG  >  CP009273/1698194‑1698492

aCGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATc                                                                                                                                                       >  2:3477885/1‑150 (MQ=255)

  tgTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGAATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTAACCGCCTCAAGCGACGCTTTGGCTAACGGGAAATGGGTATAGt                                                                                                                                                     >  1:626934/2‑150 (MQ=255)

    ttATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTg                                                                                                                                                   <  2:1002073/150‑1 (MQ=255)

     tATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGACATGGGTATCGTTgg                                                                                                                                                  >  2:4006033/1‑150 (MQ=255)

      aTGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTggg                                                                                                                                                 >  2:1421717/1‑150 (MQ=255)

       tGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGgt                                                                                                                                                <  2:232593/150‑1 (MQ=255)

        ggAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGgtg                                                                                                                                               <  2:1121843/150‑1 (MQ=255)

          aaTACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTa                                                                                                                                             <  2:2444519/150‑1 (MQ=255)

            tACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAgg                                                                                                                                           <  2:834012/150‑1 (MQ=255)

              cagacagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGaa                                                                                                                                         <  2:372530/150‑1 (MQ=255)

               agacagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAAt                                                                                                                                        >  2:3022865/1‑150 (MQ=255)

                 acagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGa                                                                                                                                      <  2:2772176/150‑1 (MQ=255)

                 acagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGa                                                                                                                                      >  1:2677518/1‑150 (MQ=255)

                  cagCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGAc                                                                                                                                     <  1:67177/150‑1 (MQ=255)

                     cacaCGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAAt                                                                                                                                  <  2:1531088/150‑1 (MQ=255)

                     cacaCGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAAt                                                                                                                                  >  2:1819918/1‑150 (MQ=255)

                      acacGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATc                                                                                                                                 >  2:2977356/1‑150 (MQ=255)

                       cacGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACCGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCa                                                                                                                                <  2:3809855/150‑1 (MQ=255)

                             ccAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTa                                                                                                                          <  2:1759040/150‑1 (MQ=255)

                              cAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAATGTTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTAt                                                                                                                         <  1:2603568/150‑1 (MQ=255)

                              cAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTAt                                                                                                                         >  2:2702991/1‑150 (MQ=255)

                               aGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATg                                                                                                                        <  2:4175909/150‑1 (MQ=255)

                                 gCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGtt                                                                                                                      >  2:26501/1‑150 (MQ=255)

                                    ttttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg                                                                                                                   <  1:1708776/150‑1 (MQ=255)

                                    ttttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg                                                                                                                   <  1:159152/150‑1 (MQ=255)

                                    ttttGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTggg                                                                                                                   <  1:1865447/150‑1 (MQ=255)

                                          cAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGtt                                                                                                             <  1:397641/150‑1 (MQ=255)

                                          cAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGtt                                                                                                             <  2:1941891/150‑1 (MQ=255)

                                           aGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTa                                                                                                            >  1:1863043/1‑150 (MQ=255)

                                              gcATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAAc                                                                                                         <  1:2604743/150‑1 (MQ=255)

                                              gcATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAAc                                                                                                         <  1:4123438/150‑1 (MQ=255)

                                              gcATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAAc                                                                                                         >  2:3080271/1‑150 (MQ=255)

                                               cATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACa                                                                                                        >  2:2189603/1‑150 (MQ=255)

                                                aTCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAg                                                                                                       <  2:3826235/150‑1 (MQ=255)

                                                aTCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAg                                                                                                       <  2:1840990/150‑1 (MQ=255)

                                                 tCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGa                                                                                                      >  1:2954215/1‑150 (MQ=255)

                                                   cAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATg                                                                                                    <  2:395177/150‑1 (MQ=255)

                                                      ctctCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCtt                                                                                                 <  2:3237798/150‑1 (MQ=255)

                                                       tctcGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTc                                                                                                <  2:461277/150‑1 (MQ=255)

                                                          cgcgCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGc                                                                                             >  2:1280952/1‑150 (MQ=255)

                                                            cgcTTGTGACAGTGTCACGGTAAAGGGTTTATCACCGACCACCTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTATATGTTGGGATCGTTAAACAGATGCTTCGGCTc                                                                                           <  2:3317415/150‑1 (MQ=255)

                                                             gcTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCa                                                                                          >  2:1191163/1‑150 (MQ=255)

                                                               ttGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCaga                                                                                        <  2:151917/150‑1 (MQ=255)

                                                                  tgACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGAc                                                                                     >  2:4174663/1‑150 (MQ=255)

                                                                  tgACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTATACAGATGCTTCGGCTCAGAGAc                                                                                     >  2:2419350/1‑150 (MQ=255)

                                                                  tgACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGAc                                                                                     <  1:1874655/150‑1 (MQ=255)

                                                                   gACAGTGCCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACa                                                                                    >  2:3783556/1‑150 (MQ=255)

                                                                      aGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACaacc                                                                                 <  1:3354793/150‑1 (MQ=255)

                                                                      aGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACaacc                                                                                 >  1:3966314/1‑150 (MQ=255)

                                                                          tCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACaaccgtaa                                                                             <  2:2359093/150‑1 (MQ=255)

                                                                            aCGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACaaccgtaacc                                                                           >  1:1933555/1‑150 (MQ=255)

                                                                             cGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACaaccgtaaccg                                                                          <  1:2768948/150‑1 (MQ=255)

                                                                                      gTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGt                                                                 <  1:2286139/150‑1 (MQ=255)

                                                                                      gTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGt                                                                 >  2:577272/1‑150 (MQ=255)

                                                                                       tttATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTc                                                                <  1:2437694/150‑1 (MQ=255)

                                                                                        ttATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCg                                                               >  1:2330717/1‑150 (MQ=255)

                                                                                        ttATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCg                                                               <  2:2570735/150‑1 (MQ=255)

                                                                                        ttATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCg                                                               <  2:3725025/150‑1 (MQ=255)

                                                                                          aTCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGc                                                             >  2:1340253/1‑150 (MQ=255)

                                                                                              aCGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCtttt                                                         <  2:3924500/150‑1 (MQ=255)

                                                                                               cGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTa                                                        >  2:1883984/1‑150 (MQ=255)

                                                                                                gACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTAc                                                       >  2:3121613/1‑150 (MQ=255)

                                                                                                   cacaTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTCAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACttt                                                    >  2:369134/1‑150 (MQ=255)

                                                                                                   cacaTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACttt                                                    <  1:571902/150‑1 (MQ=255)

                                                                                                     caTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTg                                                  <  1:1959705/150‑1 (MQ=255)

                                                                                                      aTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGt                                                 >  2:3624596/1‑150 (MQ=255)

                                                                                                      aTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGt                                                 >  1:822442/1‑150 (MQ=255)

                                                                                                      aTGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGt                                                 >  1:1156257/1‑150 (MQ=255)

                                                                                                        gTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGttt                                               >  1:880598/1‑150 (MQ=255)

                                                                                                         tttACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTtc                                              <  2:1182742/150‑1 (MQ=255)

                                                                                                          ttACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTtca                                             <  1:2629544/150‑1 (MQ=255)

                                                                                                           tACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTtcat                                            <  2:491071/150‑1 (MQ=255)

                                                                                                           tACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTtcat                                            <  1:3624477/150‑1 (MQ=255)

                                                                                                            aCCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTtcatc                                           <  2:3297199/150‑1 (MQ=255)

                                                                                                              ccGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCAc                                         >  1:91454/1‑150 (MQ=255)

                                                                                                                gCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCActg                                       <  1:3233240/150‑1 (MQ=255)

                                                                                                                gCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCActg                                       <  2:3382496/150‑1 (MQ=255)

                                                                                                                    cAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTgg                                   <  2:2012235/150‑1 (MQ=255)

                                                                                                                     aaGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGGTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGGTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGga                                  >  2:72208/1‑150 (MQ=255)

                                                                                                                      aGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGgag                                 <  1:2095549/150‑1 (MQ=255)

                                                                                                                      aGCGCCGCTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGgag                                 >  2:919744/1‑150 (MQ=255)

                                                                                                                      aGCGCCGCTTTGGCAAACGGGAAAAGGGAATAGTTGGGTGTAGGAATGACAATCAGGACAATGATTGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGATTGCAACTCAGCTTTAACTTTTGTTTCATCACTGCAGgag                                 >  2:1650388/1‑150 (MQ=255)

                                                                                                                        cgccgcTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGAt                               <  1:2691495/150‑1 (MQ=255)

                                                                                                                        cgccgcTTTGGCTAACGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGGTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGAt                               <  2:2098349/150‑1 (MQ=255)

                                                                                                                          ccgcTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTa                             <  2:1562866/150‑1 (MQ=255)

                                                                                                                           cgcTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTAc                            <  2:758504/150‑1 (MQ=255)

                                                                                                                              tttGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGc                         >  1:1373861/1‑150 (MQ=255)

                                                                                                                                tGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCa                       <  1:3410792/150‑1 (MQ=255)

                                                                                                                                 ggCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAg                      >  1:1450246/1‑150 (MQ=255)

                                                                                                                                    tAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTc                   <  1:2833012/150‑1 (MQ=255)

                                                                                                                                    tAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTc                   >  1:1986125/1‑150 (MQ=255)

                                                                                                                                     aaCGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTcc                  >  2:2167326/1‑150 (MQ=255)

                                                                                                                                      aCGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCt                 <  1:4028776/150‑1 (MQ=255)

                                                                                                                                      aCGGGAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCt                 <  2:534280/150‑1 (MQ=255)

                                                                                                                                       cGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTg                <  2:3100514/150‑1 (MQ=255)

                                                                                                                                        gggAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTGc               >  1:1410578/1‑150 (MQ=255)

                                                                                                                                         ggAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAAAAGATGCTTCGGCTCATAGACAACCGTAACCGTTTGCCATACGGCTTTTACTTTAGTTTCATCACTTCTGGAGATTACCGCCATATCCTGca              >  2:4172600/1‑149 (MQ=255)

                                                                                                                                          gAAATGGGTATAGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTGccc             <  1:3141227/150‑1 (MQ=255)

                                                                                                                                           aaaTGGGTATAGTTGGGAGTAGTAATGAAAATCATGTCTATGTTTGGATCGTTAAACAGATGCATCGGCTCAGAGAAAACCGTAACCTATGGCCAGTAGGCTTTTACTTTTGTTTCATAACAGCTGGAGATTACCGCCAGTTCCTGCCCg            >  2:2089336/1‑150 (MQ=255)

                                                                                                                                              tGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTGCCCGGGc         >  2:285617/1‑150 (MQ=255)

ACGGTTATGGAATACAGACAGCACACGCCCCAGGCTTTTTGCCAGCGCATCCAGCTCTCGCGCTTGTGACAGTGTCACGGTAAAGGGTTTATCAACGACCACATGTTTACCCGCCTCAAGCGCCGCTTTGGCTAACGGGAAATGGGTATCGTTGGGTGTAGGAATGACAATCAGGTCTATGTTGGGATCGTTAAACAGATGCTTCGGCTCAGAGACAACCGTAACCGTTGGCCAGTCGGCTTTTACTTTTGTTTCATCACTGCTGGAGATTACCGCCAGTTCCTGCCCGGGCGTGCCCG  >  CP009273/1698194‑1698492

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`