BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,753,293	G→T	40.5%	V85V (GTC→GTA)	sufE ←	sulfur acceptor protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,753,293	0	G	T	40.5%	217.3 / 231.7	205	V85V (GTC→GTA)	sufE	sulfur acceptor protein
Reads supporting (aligned to +/- strand): ref base G (51/71); new base T (43/40); total (94/111)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.35e-01

Only 100 of 216 total aligned reads displayed.

GCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTCGCGTAATT  >  CP009273/1753144‑1753441

gCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAg                                                                                                                                                      <  1:3398182/150‑1 (MQ=255)

gCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAg                                                                                                                                                      <  1:3141965/150‑1 (MQ=255)

gCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAg                                                                                                                                                      <  1:2136349/150‑1 (MQ=255)

  ggCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAtac                                                                                                                                                    >  2:2942704/1‑150 (MQ=255)

  ggCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAtac                                                                                                                                                    >  2:3641019/1‑150 (MQ=255)

      ttGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGAcc                                                                                                                                                <  1:3320657/150‑1 (MQ=255)

       tGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCg                                                                                                                                               >  2:3803435/1‑150 (MQ=255)

       tGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCg                                                                                                                                               <  2:3755629/150‑1 (MQ=255)

        ggCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGc                                                                                                                                              >  1:2636561/1‑150 (MQ=255)

            cgAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATa                                                                                                                                          >  1:1360874/1‑150 (MQ=255)

             gAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATaa                                                                                                                                         >  1:2244374/1‑150 (MQ=255)

               aTTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGc                                                                                                                                       >  1:1956947/1‑150 (MQ=255)

               aTTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGc                                                                                                                                       <  2:1310518/150‑1 (MQ=255)

                ttGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGcc                                                                                                                                      <  2:4096119/150‑1 (MQ=255)

                 tGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGccc                                                                                                                                     <  2:2192968/150‑1 (MQ=255)

                   cgcgAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCtt                                                                                                                                   >  1:37526/1‑150 (MQ=255)

                   cgcgAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCtt                                                                                                                                   <  2:1939015/150‑1 (MQ=255)

                     cgAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTc                                                                                                                                 <  2:3130010/150‑1 (MQ=255)

                      gAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTca                                                                                                                                >  1:1018642/1‑150 (MQ=255)

                          catcGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAAt                                                                                                                            >  1:2376260/1‑150 (MQ=255)

                           atcGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATc                                                                                                                           >  2:2663048/1‑150 (MQ=255)

                           atcGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATc                                                                                                                           <  1:2745486/150‑1 (MQ=255)

                             cGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATcgc                                                                                                                         >  2:1717209/1‑150 (MQ=255)

                               cTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATcgccg                                                                                                                       >  1:3249865/1‑150 (MQ=255)

                                  ccAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCAt                                                                                                                    <  1:1369541/150‑1 (MQ=255)

                                  ccAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCAt                                                                                                                    >  1:4018255/1‑150 (MQ=255)

                                   cAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATc                                                                                                                   <  1:181501/150‑1 (MQ=255)

                                     gACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGc                                                                                                                 >  2:606465/1‑150 (MQ=255)

                                      aCCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCt                                                                                                                <  1:3311909/150‑1 (MQ=255)

                                      aCCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCt                                                                                                                <  1:2032454/150‑1 (MQ=255)

                                        cTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGt                                                                                                              <  2:903005/150‑1 (MQ=255)

                                           gtgAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGc                                                                                                           >  2:3644090/1‑150 (MQ=255)

                                           gtgAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGc                                                                                                           >  2:2127317/1‑150 (MQ=255)

                                            tgAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGcc                                                                                                          >  1:2414350/1‑150 (MQ=255)

                                              aaCGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCt                                                                                                        <  2:3723735/150‑1 (MQ=255)

                                              aaCGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCt                                                                                                        <  2:573660/150‑1 (MQ=255)

                                               aCGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTg                                                                                                       <  2:2901769/150‑1 (MQ=255)

                                               aCGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTg                                                                                                       >  2:2545069/1‑150 (MQ=255)

                                                 gagaTGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTa                                                                                                     >  2:3324254/1‑150 (MQ=255)

                                                  agaTGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTaa                                                                                                    <  2:3857205/150‑1 (MQ=255)

                                                    aTGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAAtt                                                                                                  >  1:1172617/1‑150 (MQ=255)

                                                      ggggTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCa                                                                                                >  1:911640/1‑150 (MQ=255)

                                                           gagaTGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCaataat                                                                                           <  1:3266098/150‑1 (MQ=255)

                                                            agaTGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAAtt                                                                                          >  1:2392563/1‑150 (MQ=255)

                                                                gTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCt                                                                                      <  1:3843139/150‑1 (MQ=255)

                                                                 ttGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATNGCTGTCGCCCTGTAATTCAATAATTCCCTg                                                                                     <  1:3855071/150‑1 (MQ=255)

                                                                  tGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTgg                                                                                    <  2:2569818/150‑1 (MQ=255)

                                                                  tGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTgg                                                                                    <  1:1548386/150‑1 (MQ=255)

                                                                    ggTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGc                                                                                  <  2:2263155/150‑1 (MQ=255)

                                                                     gTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCa                                                                                 <  2:2723899/150‑1 (MQ=255)

                                                                        aGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTc                                                                              <  1:1094227/150‑1 (MQ=255)

                                                                         gcgcCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCt                                                                             <  2:460696/150‑1 (MQ=255)

                                                                          cgcCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTg                                                                            <  2:397293/150‑1 (MQ=255)

                                                                               ttttttCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCa                                                                       <  2:1228252/150‑1 (MQ=255)

                                                                                 ttttCAAACCACGGACGGACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATg                                                                     <  2:1590057/150‑1 (MQ=255)

                                                                                  tttCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGa                                                                    <  2:3312318/150‑1 (MQ=255)

                                                                                  tttCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGa                                                                    >  2:1605627/1‑150 (MQ=255)

                                                                                    tCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACa                                                                  <  2:3799746/150‑1 (MQ=255)

                                                                                    tCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACa                                                                  <  2:3198439/150‑1 (MQ=255)

                                                                                      aaaCCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAAt                                                                >  2:338076/1‑150 (MQ=255)

                                                                                      aaaCCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAAt                                                                >  2:2859087/1‑150 (MQ=255)

                                                                                       aaCCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATc                                                               >  2:4154110/1‑150 (MQ=255)

                                                                                           aCGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCcaca                                                           >  2:1357241/1‑150 (MQ=255)

                                                                                            cGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCacac                                                          >  1:655463/1‑150 (MQ=255)

                                                                                              gACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCt                                                        <  2:1233979/150‑1 (MQ=255)

                                                                                                     aTCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTg                                                 >  2:3512363/1‑150 (MQ=255)

                                                                                                       cGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACTACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGAc                                               <  1:2838072/150‑1 (MQ=255)

                                                                                                       cGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGAc                                               >  1:1397749/1‑150 (MQ=255)

                                                                                                       cGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGAc                                               <  1:3283506/150‑1 (MQ=255)

                                                                                                       cGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGAc                                               <  1:2838115/150‑1 (MQ=255)

                                                                                                        gAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACa                                              <  1:1197679/150‑1 (MQ=255)

                                                                                                             tGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCt                                         <  2:801619/150‑1 (MQ=255)

                                                                                                             tGACAATATCCTGCGGCGTCATCTGATAGTAGTGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCTATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCt                                         <  1:54035/150‑1 (MQ=255)

                                                                                                              gACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATAGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTg                                        >  2:3405403/1‑150 (MQ=255)

                                                                                                               aCAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGa                                       <  2:1764929/150‑1 (MQ=255)

                                                                                                               aCAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGa                                       <  2:3095200/150‑1 (MQ=255)

                                                                                                                 aaTATCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAAt                                     >  1:2580139/1‑150 (MQ=255)

                                                                                                                 aaTATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAAt                                     >  1:3806694/1‑150 (MQ=255)

                                                                                                                 aaTATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAAt                                     <  2:3326980/150‑1 (MQ=255)

                                                                                                                  atatCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATg                                    >  2:846247/1‑150 (MQ=255)

                                                                                                                  atatCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATg                                    <  1:1870002/150‑1 (MQ=255)

                                                                                                                  atatCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATg                                    <  1:1870007/150‑1 (MQ=255)

                                                                                                                  atatCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATg                                    <  1:2090897/150‑1 (MQ=255)

                                                                                                                   tatCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGc                                   >  1:2902649/1‑150 (MQ=255)

                                                                                                                    atCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCt                                  <  1:173630/150‑1 (MQ=255)

                                                                                                                     tCCTGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCtc                                 >  2:3060534/1‑149 (MQ=255)

                                                                                                                        tGCGGCGTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTAttt                              <  1:2263492/150‑1 (MQ=255)

                                                                                                                        tGCGGCGTAATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTAttt                              <  1:2473341/150‑1 (MQ=255)

                                                                                                                          cggcgTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTtg                            >  2:3079565/1‑150 (MQ=255)

                                                                                                                           ggcgTCATCTGATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTtgt                           >  1:3931431/1‑150 (MQ=255)

                                                                                                                                     gATCGTAGAGAATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGt                 >  1:3043689/1‑150 (MQ=255)

                                                                                                                                         gTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtc             <  1:624443/150‑1 (MQ=255)

                                                                                                                                           agagAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtcgt           <  1:705384/150‑1 (MQ=255)

                                                                                                                                            gagaATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtcgtc          <  2:452714/150‑1 (MQ=255)

                                                                                                                                            gagaATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtcgtc          <  2:3694867/150‑1 (MQ=255)

                                                                                                                                            gagaATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtcgtc          <  2:1004510/150‑1 (MQ=255)

                                                                                                                                            gagaATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTtcgtc          <  2:1166215/150‑1 (MQ=255)

                                                                                                                                              gaATAAATACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTcgc        <  2:2018204/150‑1 (MQ=255)

                                                                                                                                              gaATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTcgc        >  2:3520160/1‑150 (MQ=255)

                                                                                                                                                 tAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTCGCGTa     <  1:2789329/150‑1 (MQ=255)

GCGGCTTTGGCGCGAATTGCGCGAATCATCGCTTCCAGACCTTGTGAACGAGATGGGGTGAGATGTTGGGTGAGCGCCATTTTTTCAAACCACGGACGCACATCGAAATTGACAATATCCTGCGGCGTCATCTGATCGTAGAGAATAAAGACGACCGCAATAAGCCCTTTCACAATCGCCGCATCGCTGTCGCCCTGTAATTCAATAATTCCCTGGGCATTCTGGCGCATGACAATCCACACCTGACTCTGACAGCCCTGAATGCTATTTTGTGGACTTCTGTCTTCGTCGCGTAATT  >  CP009273/1753144‑1753441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`