Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,795,267 A→T 33.6% F521I (TTC→ATC)  thrS ← threonyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,795,2670AT33.6% 283.0 / 241.1 217F521I (TTC→ATC) thrSthreonyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base A (74/70);  new base T (37/36);  total (111/106)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 223 total aligned reads displayed.

TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAT  >  CP009273/1795119‑1795416
                                                                                                                                                    |                                                                                                                                                     
ttAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGaaa                                                                                                                                                      >  1:2396000/1‑150 (MQ=255)
   acacGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGt                                                                                                                                                   >  1:3283974/1‑150 (MQ=255)
     acGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTc                                                                                                                                                 <  2:3878159/150‑1 (MQ=255)
      cGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTcc                                                                                                                                                <  1:3427294/150‑1 (MQ=255)
      cGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTcc                                                                                                                                                <  1:1314197/150‑1 (MQ=255)
      cGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTcc                                                                                                                                                <  1:1502317/150‑1 (MQ=255)
        aaTGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCAt                                                                                                                                              >  1:880157/1‑150 (MQ=255)
          tGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCg                                                                                                                                            >  1:1567925/1‑150 (MQ=255)
           gCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGa                                                                                                                                           <  2:2129555/150‑1 (MQ=255)
           gCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGa                                                                                                                                           <  1:50130/150‑1 (MQ=255)
                 aTTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAg                                                                                                                                     <  1:3778347/150‑1 (MQ=255)
                   ttGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGaa                                                                                                                                   >  1:2873069/1‑150 (MQ=255)
                     gATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAAtt                                                                                                                                 <  1:237877/150‑1 (MQ=255)
                      aTAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTg                                                                                                                                >  2:1753452/1‑150 (MQ=255)
                      aTAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTg                                                                                                                                >  1:2658875/1‑150 (MQ=255)
                          tttttGCGTCAATTCGTTAACGTATTCAGACTGTTAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATAACGATGAAACGTTCCATCGACCCCAGAATTGcgcg                                                                                                                            <  1:3578626/150‑1 (MQ=255)
                             ttGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTg                                                                                                                         <  2:415765/150‑1 (MQ=255)
                              tGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGa                                                                                                                        >  1:3258424/1‑150 (MQ=255)
                              tGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGa                                                                                                                        <  2:2285983/150‑1 (MQ=255)
                               gCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGaa                                                                                                                       <  1:2506479/150‑1 (MQ=255)
                               gCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGaa                                                                                                                       <  1:3309708/150‑1 (MQ=255)
                               gCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACTTTCCATCGACCCCAGAATTGCGCGGTGaa                                                                                                                       <  1:616597/150‑1 (MQ=255)
                                cGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAAt                                                                                                                      >  1:1998056/1‑150 (MQ=255)
                                   cAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAt                                                                                                                   >  2:2636168/1‑150 (MQ=255)
                                   cAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAt                                                                                                                   <  2:271068/150‑1 (MQ=255)
                                   cAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAt                                                                                                                   <  2:1056252/150‑1 (MQ=255)
                                    aaTTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAtt                                                                                                                  >  2:1445497/1‑150 (MQ=255)
                                      ttCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTAc                                                                                                                <  2:1233398/150‑1 (MQ=255)
                                      ttCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTAc                                                                                                                <  2:2313956/150‑1 (MQ=255)
                                         gTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCgg                                                                                                             >  2:870632/1‑150 (MQ=255)
                                          ttAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGt                                                                                                            <  2:1650620/150‑1 (MQ=255)
                                           tAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTa                                                                                                           <  1:1194958/150‑1 (MQ=255)
                                            aaCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTAc                                                                                                          <  2:2245542/150‑1 (MQ=255)
                                            aaCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTAc                                                                                                          >  2:3008336/1‑150 (MQ=255)
                                            aaCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTAc                                                                                                          >  1:2299258/1‑150 (MQ=255)
                                             aCGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACt                                                                                                         <  1:804202/150‑1 (MQ=255)
                                              cGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACtt                                                                                                        <  2:199279/150‑1 (MQ=255)
                                               gTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACttt                                                                                                       <  2:496151/150‑1 (MQ=255)
                                               gTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACttt                                                                                                       <  2:3979255/150‑1 (MQ=255)
                                                      gACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCa                                                                                                >  2:2901172/1‑150 (MQ=255)
                                                       aCTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCAt                                                                                               >  1:3734584/1‑150 (MQ=255)
                                                          gtgAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGt                                                                                            <  2:1841123/150‑1 (MQ=255)
                                                          gtgAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGt                                                                                            >  2:671433/1‑150 (MQ=255)
                                                             aaTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCtt                                                                                         <  2:3986152/150‑1 (MQ=255)
                                                              aTCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTc                                                                                        <  2:2333441/150‑1 (MQ=255)
                                                               tCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCg                                                                                       <  2:137526/150‑1 (MQ=255)
                                                                cGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGc                                                                                      >  2:854259/1‑150 (MQ=255)
                                                                 ggTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGcc                                                                                     >  1:3907252/1‑150 (MQ=255)
                                                                   tAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTa                                                                                   >  2:128176/1‑150 (MQ=255)
                                                                       atTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATa                                                                               >  1:295169/1‑150 (MQ=255)
                                                                        ttCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaa                                                                              <  2:3183757/150‑1 (MQ=255)
                                                                        ttCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaa                                                                              <  1:3707400/150‑1 (MQ=255)
                                                                         tCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaag                                                                             >  2:1851775/1‑150 (MQ=255)
                                                                          cATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaaga                                                                            <  1:1683105/150‑1 (MQ=255)
                                                                          cATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaaga                                                                            >  1:2928988/1‑150 (MQ=255)
                                                                          cATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaaga                                                                            >  1:3623897/1‑150 (MQ=255)
                                                                           aTGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaa                                                                           <  2:531962/150‑1 (MQ=255)
                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                          >  2:706464/1‑150 (MQ=255)
                                                                            tGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATaagaag                                                                          <  1:3886702/150‑1 (MQ=255)
                                                                              aTAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgcg                                                                        >  1:1016973/1‑150 (MQ=255)
                                                                              aTAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgcg                                                                        >  1:3439832/1‑150 (MQ=255)
                                                                              aTAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgcg                                                                        >  2:371703/1‑150 (MQ=255)
                                                                               tAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAgcgc                                                                       >  1:3533467/1‑150 (MQ=255)
                                                                                aacaacGTGAACCTGCGCAAGCCAGGTCGGGAAGAAACCTGCGAACTCTTCGGTCAGGTTACCGATGAAACGTTCCATCGTGCCCAGTATTGCGCGGTTAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTTCATAAGAAGCGCt                                                                      <  2:2304044/150‑1 (MQ=255)
                                                                                aacaacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCt                                                                      >  2:2954965/1‑150 (MQ=255)
                                                                                   aacCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCag                                                                   >  1:54414/1‑150 (MQ=255)
                                                                                     ccTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagac                                                                 >  1:637376/1‑150 (MQ=255)
                                                                                     ccTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagac                                                                 <  2:3574035/150‑1 (MQ=255)
                                                                                      cTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacg                                                                <  1:1734760/150‑1 (MQ=255)
                                                                                         aaCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACCTAAGAAGCGCTCagacgaga                                                             >  2:3464678/1‑150 (MQ=255)
                                                                                         aaCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgaga                                                             >  1:3073916/1‑150 (MQ=255)
                                                                                          aCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgagac                                                            <  1:607410/150‑1 (MQ=255)
                                                                                          aCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgagac                                                            >  1:2791679/1‑150 (MQ=255)
                                                                                          aCCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgagac                                                            <  2:2069361/150‑1 (MQ=255)
                                                                                           ccGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCagacgagacg                                                           <  1:2128912/150‑1 (MQ=255)
                                                                                              gcgcAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCa                                                        >  1:3647622/1‑150 (MQ=255)
                                                                                                  aaGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAaga                                                    >  2:550136/1‑150 (MQ=255)
                                                                                                   aGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAagag                                                   <  1:3200785/150‑1 (MQ=255)
                                                                                                    gCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAgaga                                                  <  1:245833/150‑1 (MQ=255)
                                                                                                      cAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAg                                                >  2:2223062/1‑150 (MQ=255)
                                                                                                      cAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAg                                                >  2:350713/1‑150 (MQ=255)
                                                                                                       atgTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCAATACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCGCAGACGAGACGGCAAAGAGAAGt                                               >  2:3125114/3‑150 (MQ=255)
                                                                                                        ggTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTc                                              >  1:3386368/1‑150 (MQ=255)
                                                                                                               aagaaACCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgt                                       <  1:3929341/150‑1 (MQ=255)
                                                                                                               aagaaACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgt                                       >  2:2878850/1‑150 (MQ=255)
                                                                                                                  aaaCCAGCGAACTCTTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGATGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGctgtact                                    >  1:2742855/1‑150 (MQ=255)
                                                                                                                      cAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCtgtactgtac                                <  1:2455429/150‑1 (MQ=255)
                                                                                                                           aaCTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCAc                           <  1:1052545/150‑1 (MQ=255)
                                                                                                                               ctTCGGTCAGGATACCGATGATACGTTCCATCGACCCCAGACTTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGcc                       <  2:304502/150‑1 (MQ=255)
                                                                                                                                   ggTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                   >  1:2535663/1‑150 (MQ=255)
                                                                                                                                   ggTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                   >  1:2173596/1‑150 (MQ=255)
                                                                                                                                   ggTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGc                   >  1:124650/1‑150 (MQ=255)
                                                                                                                                    gTCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCa                  >  1:1466946/1‑150 (MQ=255)
                                                                                                                                     tCAGGATACCGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAATTCCAGCTGTACTGTACCGCACTGCCATGCAc                 >  1:2760863/1‑150 (MQ=255)
                                                                                                                                         gATTCCGATGATAAGTTCCATCGACCCCAGTATTTCGCGGTGAATCATTACCTGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTTCTGTACCGCACTGCCATGCACGATc             <  1:2907055/150‑1 (MQ=255)
                                                                                                                                         gATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATc             <  2:3970144/150‑1 (MQ=255)
                                                                                                                                            aCCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAg          <  1:1177776/150‑1 (MQ=255)
                                                                                                                                             ccGATGATACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAgg         <  1:758218/150‑1 (MQ=255)
                                                                                                                                                  gAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTc    >  2:3887040/1‑150 (MQ=255)
                                                                                                                                                   aaaCGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCa   <  1:3133052/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACAACCTGAACCGGCGCAAGCCAGGTCGGGAAGAAACCAGCGAACTCTTCGGTCAGGATACCGATGAAACGTTCCATCGACCCCAGAATTGCGCGGTGAATCATTACCGGTACTTTACGTTCATTGTCTTCGCCTACATAAGAAGCGCTCAGACGAGACGGCAAAGAGAAGTCCAGCTGTACTGTACCGCACTGCCATGCACGATCGAGGCAGTCAT  >  CP009273/1795119‑1795416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑