Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,825,763 C→A 100% A159A (GCG→GCT)  astC ← succinylornithine transaminase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,825,7630CA95.1% 721.7 / 26.0 224A159A (GCG→GCT) astCsuccinylornithine transaminase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (10/1);  new base A (118/95);  total (128/96)
Fisher's exact test for biased strand distribution p-value = 2.61e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

CCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGC  >  CP009273/1825617‑1825909
                                                                                                                                                  |                                                                                                                                                  
ccTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCa                                                                                                                                                 <  1:414467/150‑1 (MQ=255)
    gTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                             >  2:2813340/1‑150 (MQ=255)
    gTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAAt                                                                                                                                             >  1:155690/1‑150 (MQ=255)
      aaaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATcc                                                                                                                                           >  2:2146410/1‑150 (MQ=255)
       aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCt                                                                                                                                          >  2:339529/1‑150 (MQ=255)
       aaaaCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCt                                                                                                                                          <  2:1758859/150‑1 (MQ=255)
          aCGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCGGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCtgtg                                                                                                                                       <  2:3082520/150‑1 (MQ=255)
           cgcgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGa                                                                                                                                      >  2:492125/1‑150 (MQ=255)
             cgTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAAt                                                                                                                                    <  2:1074812/150‑1 (MQ=255)
             cgTTGCTGGATGGCACCACACCGCCTTCCCCCTGGATGGGTTAGACAAACACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAAt                                                                                                                                    >  1:1242180/1‑150 (MQ=255)
                   tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                              >  2:310772/1‑150 (MQ=255)
                   tggctggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAggctg                                                                                                                              >  1:144417/1‑150 (MQ=255)
                       tggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGgctggctg                                                                                                                          >  2:3411608/1‑150 (MQ=255)
                        ggcACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGc                                                                                                                         >  1:1771135/1‑150 (MQ=255)
                           accacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCa                                                                                                                      >  1:52404/1‑150 (MQ=255)
                            ccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAc                                                                                                                     <  2:3970729/150‑1 (MQ=255)
                            ccacACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAc                                                                                                                     <  2:408565/150‑1 (MQ=255)
                             cacaCCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAcc                                                                                                                    >  1:1462324/1‑150 (MQ=255)
                              acacCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCAccc                                                                                                                   >  2:1847407/1‑150 (MQ=255)
                                 ccGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCa                                                                                                                <  1:3564326/150‑1 (MQ=255)
                                  cGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCAc                                                                                                               >  1:3811246/1‑150 (MQ=255)
                                     cTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGa                                                                                                            >  2:2188664/1‑150 (MQ=255)
                                             tGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                    >  2:253424/1‑150 (MQ=255)
                                             tGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAc                                                                                                    >  1:3974200/1‑150 (MQ=255)
                                              ggATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACa                                                                                                   <  2:2254229/150‑1 (MQ=255)
                                                aTGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGc                                                                                                 <  2:2549694/150‑1 (MQ=255)
                                                  gggTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGt                                                                                               >  1:3084986/1‑150 (MQ=255)
                                                  gggTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGt                                                                                               <  1:1989900/150‑1 (MQ=255)
                                                        gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAcc                                                                                         <  2:1166383/150‑1 (MQ=255)
                                                        gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAcc                                                                                         >  2:2031243/1‑150 (MQ=255)
                                                        gACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGAcc                                                                                         >  2:3183177/1‑150 (MQ=255)
                                                         aCAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCa                                                                                        <  1:408240/150‑1 (MQ=255)
                                                          cAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCAt                                                                                       >  1:4009311/1‑150 (MQ=255)
                                                            aTCACCGCACAGGTCGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                     >  2:1159269/1‑150 (MQ=255)
                                                            aTCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGa                                                                                     >  2:1372289/1‑150 (MQ=255)
                                                             tCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGaa                                                                                    <  2:630507/150‑1 (MQ=255)
                                                               aCCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAAc                                                                                  <  2:398514/150‑1 (MQ=255)
                                                                   cacaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAt                                                                              <  2:3880095/150‑1 (MQ=255)
                                                                   cacaGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAt                                                                              >  1:938685/1‑150 (MQ=255)
                                                                     caGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttt                                                                            >  2:2239113/1‑150 (MQ=255)
                                                                     caGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttt                                                                            <  1:837297/150‑1 (MQ=255)
                                                                      aGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGACAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAtttt                                                                           <  1:26422/150‑1 (MQ=255)
                                                                       ggTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCAttttt                                                                          >  1:92943/1‑150 (MQ=255)
                                                                        gTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTg                                                                         <  1:1320140/150‑1 (MQ=255)
                                                                        gTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTg                                                                         >  1:1165133/1‑150 (MQ=255)
                                                                         tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                        >  2:1669454/1‑150 (MQ=255)
                                                                         tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                        <  2:863413/150‑1 (MQ=255)
                                                                         tAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGa                                                                        >  1:2560027/1‑150 (MQ=255)
                                                                          agagTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGaa                                                                       >  2:4052621/1‑150 (MQ=255)
                                                                             gtcgtcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGc                                                                    >  2:323233/1‑150 (MQ=255)
                                                                                 tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACg                                                                >  1:768792/1‑150 (MQ=255)
                                                                                 tcAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACg                                                                >  1:3124725/1‑150 (MQ=255)
                                                                                   aaTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                              >  1:2150624/1‑150 (MQ=255)
                                                                                   aaTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGAt                                                              >  1:3318363/1‑150 (MQ=255)
                                                                                    aTCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATg                                                             >  1:2506869/1‑150 (MQ=255)
                                                                                          gcgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctc                                                       <  2:4172683/150‑1 (MQ=255)
                                                                                           cgcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGctct                                                      >  1:831955/1‑150 (MQ=255)
                                                                                            gcTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCtt                                                     >  2:2534156/1‑150 (MQ=255)
                                                                                             cTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTa                                                    <  2:10078/150‑1 (MQ=255)
                                                                                              tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                   >  1:3485424/1‑150 (MQ=255)
                                                                                              tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                   >  2:3596017/1‑150 (MQ=255)
                                                                                              tGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTAt                                                   >  2:20999/1‑150 (MQ=255)
                                                                                                 cAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGc                                                >  1:3773188/1‑150 (MQ=255)
                                                                                                  agagTTACTATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCt                                               <  1:2847099/150‑1 (MQ=255)
                                                                                                    agTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAAAAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGc                                             >  2:256685/1‑150 (MQ=255)
                                                                                                     gTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGcc                                            <  1:1432948/150‑1 (MQ=255)
                                                                                                       tAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGt                                          >  1:3884509/1‑150 (MQ=255)
                                                                                                        aaTATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                         >  1:3209213/1‑150 (MQ=255)
                                                                                                        aaTATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTa                                         >  1:1850720/1‑150 (MQ=255)
                                                                                                         atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAg                                        >  2:2021608/1‑150 (MQ=255)
                                                                                                         atatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAg                                        >  2:2021423/1‑150 (MQ=255)
                                                                                                          tatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                       <  2:2925792/150‑1 (MQ=255)
                                                                                                          tatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                       <  1:4128090/150‑1 (MQ=255)
                                                                                                          tatCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGc                                       <  1:261438/150‑1 (MQ=255)
                                                                                                              gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                   >  1:1436396/1‑150 (MQ=255)
                                                                                                              gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                   <  2:3424157/150‑1 (MQ=255)
                                                                                                              gTTATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTc                                   <  1:2326260/150‑1 (MQ=255)
                                                                                                               ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCg                                  <  2:1913319/150‑1 (MQ=255)
                                                                                                               ttATATGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTAATGGCTGCCGTAGCGGTCg                                  >  2:2745336/1‑150 (MQ=255)
                                                                                                                tataTGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGt                                 <  1:2747019/150‑1 (MQ=255)
                                                                                                                 atatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                                >  1:67063/1‑150 (MQ=255)
                                                                                                                 atatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTg                                >  1:3763487/1‑150 (MQ=255)
                                                                                                                  tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGa                               <  2:2613527/150‑1 (MQ=255)
                                                                                                                  tatGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGa                               >  2:1583428/1‑150 (MQ=255)
                                                                                                                   atGCAGCATGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAg                              <  2:2948713/150‑1 (MQ=255)
                                                                                                                       agcaTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGaa                          >  1:1638849/1‑150 (MQ=255)
                                                                                                                         caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                        <  2:88183/150‑1 (MQ=255)
                                                                                                                         caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                        <  1:2962154/150‑1 (MQ=255)
                                                                                                                         caTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAtt                        <  1:2171878/150‑1 (MQ=255)
                                                                                                                          aTGACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAAttt                       <  1:704457/150‑1 (MQ=255)
                                                                                                                            gACGAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAc                     <  1:3434164/150‑1 (MQ=255)
                                                                                                                               gAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgcg                  >  2:1341088/1‑150 (MQ=255)
                                                                                                                               gAATATCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTAcgcg                  >  2:589038/1‑150 (MQ=255)
                                                                                                                                 atatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGcc                >  1:2834176/1‑150 (MQ=255)
                                                                                                                                 atatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGcc                >  1:1058830/1‑150 (MQ=255)
                                                                                                                                  tatCCGCCGGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCa               >  1:149463/1‑150 (MQ=255)
                                                                                                                                        ccgGCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTa         <  1:1017558/150‑1 (MQ=255)
                                                                                                                                          ggCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAgc       >  1:3017395/1‑150 (MQ=255)
                                                                                                                                           gCAGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAgcg      >  2:3364128/1‑150 (MQ=255)
                                                                                                                                             aGTGGAGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGcgcc    <  2:1518447/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CCTTGTAAAAACGCGTTGCTGGCTGGCACCACACCGCCTTCCCCCTGGATGGGTTCGACAATCACCGCACAGGTAGAGTCGTCAATCAGCGCGCTGGCAGAGTTAATATCGTTATATGCAGCATGACGAATATCCGCCGGCAGTGGCGCAAAATCCTGTGAATAGGCTGGCTGCCCACCCGCACTGACAGTAAACAGCGTGCGACCATGAAACGCATTTTTGAACGCCACGATGCCGCTCTTATGGCTGCCGTAGCGGTCGTGAGCGAATTTACGCGCCAGTTTTAGCGCCGC  >  CP009273/1825617‑1825909

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑