BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,842,604	A→T	5.8%	S110S (TCT→TCA)	ydjA ←	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,842,604	0	A	T	5.8%	874.1 / 31.4	257	S110S (TCT→TCA)	ydjA	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (112/130); new base T (3/12); total (115/142)
Fisher's exact test for biased strand distribution p-value = 6.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 259 total aligned reads displayed.

AGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTAC  >  CP009273/1842455‑1842753

aGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCa                                                                                                                                                       >  1:522253/1‑150 (MQ=255)

   tAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGAc                                                                                                                                                    >  1:4108593/1‑150 (MQ=255)

     gagaAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAt                                                                                                                                                  >  2:3325913/1‑150 (MQ=255)

     gagaAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACAt                                                                                                                                                  <  2:3055837/150‑1 (MQ=255)

        aaaaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTc                                                                                                                                               >  1:1037390/1‑150 (MQ=255)

          aaCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCt                                                                                                                                             <  1:1618840/150‑1 (MQ=255)

           aCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTg                                                                                                                                            >  1:2241601/1‑150 (MQ=255)

           aCCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTg                                                                                                                                            <  1:1714003/150‑1 (MQ=255)

             cGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGtt                                                                                                                                          >  2:1477055/1‑150 (MQ=255)

             cGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGtt                                                                                                                                          >  2:1005107/1‑150 (MQ=255)

              gACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCTGACATTTCCTGTTc                                                                                                                                         <  2:87630/150‑1 (MQ=255)

              gACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTc                                                                                                                                         <  1:248330/150‑1 (MQ=255)

                 aaTTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCa                                                                                                                                      <  1:2235364/150‑1 (MQ=255)

                 aaTTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCa                                                                                                                                      >  2:3789426/1‑150 (MQ=255)

                        tCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCggg                                                                                                                               >  1:1085122/1‑150 (MQ=255)

                         ccTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGa                                                                                                                              >  2:1169432/1‑150 (MQ=255)

                         ccTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGa                                                                                                                              <  2:499359/150‑1 (MQ=255)

                          cTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGAc                                                                                                                             <  1:701451/150‑1 (MQ=255)

                          cTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGAc                                                                                                                             <  1:1651604/150‑1 (MQ=255)

                          cTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGAc                                                                                                                             <  2:2223866/150‑1 (MQ=255)

                           tGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACt                                                                                                                            >  1:1131779/1‑150 (MQ=255)

                           tGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACt                                                                                                                            <  2:2634922/150‑1 (MQ=255)

                             cTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACttt                                                                                                                          >  1:2158008/1‑150 (MQ=255)

                             cTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACttt                                                                                                                          >  2:1678365/1‑150 (MQ=255)

                                  ggCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGAt                                                                                                                     >  2:3239054/1‑150 (MQ=255)

                                    cAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCTGACATTTCCTGTTCCCAGCGCGGGACTTTATGATtc                                                                                                                   >  1:386881/1‑150 (MQ=255)

                                    cAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATtc                                                                                                                   <  2:3472007/150‑1 (MQ=255)

                                     aaCCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATtct                                                                                                                  >  1:2317222/1‑150 (MQ=255)

                                     aaCCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATtct                                                                                                                  >  2:170292/1‑150 (MQ=255)

                                       ccGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTctct                                                                                                                <  1:1820590/150‑1 (MQ=255)

                                       ccGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTctct                                                                                                                >  2:2842842/1‑150 (MQ=255)

                                         gAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTc                                                                                                              >  2:2677972/1‑150 (MQ=255)

                                          aaTGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCg                                                                                                             <  2:1738994/150‑1 (MQ=255)

                                             gCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCAt                                                                                                          <  2:3993149/150‑1 (MQ=255)

                                               ttCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCAttt                                                                                                        <  1:3124744/150‑1 (MQ=255)

                                                tCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTc                                                                                                       <  1:2027217/150‑1 (MQ=255)

                                                         aCCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCaccacc                                                                                              <  1:1376685/150‑1 (MQ=255)

                                                           cGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGt                                                                                            >  1:2008052/1‑150 (MQ=255)

                                                              aCTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgat                                                                                         <  1:1006658/150‑1 (MQ=255)

                                                                tttCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGtgatga                                                                                       <  1:864828/150‑1 (MQ=255)

                                                                 ttcaaatAATGCGCCACACAGCCATATTACGACAAACCCCTGGGCAACTGCTGCCAATAGCATCGCCATCACCGCGCAACCGGCTGACATTTCCTGTTCCCATCGCTGGACATTATGAATCACTTCGCAGATCGCCACCACCGtgatgat                                                                                      >  2:1387679/7‑150 (MQ=255)

                                                                     gTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGc                                                                                  >  1:3962686/1‑150 (MQ=255)

                                                                      ttAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCg                                                                                 >  1:3706690/1‑150 (MQ=255)

                                                                            cgcCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCTGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTgcgc                                                                           <  2:3952422/150‑1 (MQ=255)

                                                                              ccACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCgg                                                                         >  1:1247450/1‑150 (MQ=255)

                                                                               cACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCTCCATGACCGCGCATCCTGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGa                                                                        <  1:812285/150‑1 (MQ=255)

                                                                               cACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGa                                                                        <  1:2901870/150‑1 (MQ=255)

                                                                               cACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGa                                                                        <  2:415525/150‑1 (MQ=255)

                                                                                aCTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGaa                                                                       >  2:1066412/1‑150 (MQ=255)

                                                                                 cTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAAc                                                                      <  1:4004931/150‑1 (MQ=255)

                                                                                 cTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAAc                                                                      <  2:3938136/150‑1 (MQ=255)

                                                                                 cTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAAc                                                                      <  2:4088650/150‑1 (MQ=255)

                                                                                  tGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACg                                                                     <  2:693916/150‑1 (MQ=255)

                                                                                  tGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACg                                                                     >  1:3606075/1‑150 (MQ=255)

                                                                                   gcgcCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACgg                                                                    >  1:1756420/1‑150 (MQ=255)

                                                                                       cAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCa                                                                >  2:3044693/1‑150 (MQ=255)

                                                                                         gATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCACCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCAtt                                                              <  2:4107625/150‑1 (MQ=255)

                                                                                          aTGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTa                                                             >  1:1852957/1‑150 (MQ=255)

                                                                                          aTGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTa                                                             <  2:2975755/150‑1 (MQ=255)

                                                                                                 cAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCtt                                                      >  1:473041/1‑150 (MQ=255)

                                                                                                    aCCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGt                                                   >  1:3527601/1‑150 (MQ=255)

                                                                                                    aCCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGt                                                   <  2:2392853/150‑1 (MQ=255)

                                                                                                      cccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCg                                                 <  1:4113054/150‑1 (MQ=255)

                                                                                                       ccTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGa                                                >  2:3974347/1‑150 (MQ=255)

                                                                                                         tGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATa                                              >  1:1305460/1‑150 (MQ=255)

                                                                                                         tGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATa                                              <  2:3546792/150‑1 (MQ=255)

                                                                                                         tGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTNTGTCGATa                                              <  1:3770388/150‑1 (MQ=255)

                                                                                                          gggCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAg                                             <  2:3880101/150‑1 (MQ=255)

                                                                                                           ggCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGc                                            >  1:2806016/1‑150 (MQ=255)

                                                                                                            gCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCt                                           >  1:1927736/1‑150 (MQ=255)

                                                                                                             cAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCtt                                          <  1:2866480/150‑1 (MQ=255)

                                                                                                             cAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCtt                                          >  1:416318/1‑150 (MQ=255)

                                                                                                             cAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCtt                                          <  1:777983/150‑1 (MQ=255)

                                                                                                              aaCTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCttt                                         >  2:1831468/1‑150 (MQ=255)

                                                                                                               aCTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTg                                        <  2:2432951/150‑1 (MQ=255)

                                                                                                               aCTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTg                                        <  2:60137/150‑1 (MQ=255)

                                                                                                                   ctgcCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGtcat                                    <  1:1453529/150‑1 (MQ=255)

                                                                                                                      ccATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCAc                                 >  1:1542034/1‑150 (MQ=255)

                                                                                                                          ttGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTAcc                             <  1:1358342/150‑1 (MQ=255)

                                                                                                                           tGCATCGCCATGACCGCGCATCCGGCTGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCg                            <  2:757447/150‑1 (MQ=255)

                                                                                                                            gCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCgg                           <  2:3194573/150‑1 (MQ=255)

                                                                                                                               tCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcag                        <  2:42889/150‑1 (MQ=255)

                                                                                                                                 gCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcagca                      <  2:3669465/150‑1 (MQ=255)

                                                                                                                                 gCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcagca                      <  2:1278904/150‑1 (MQ=255)

                                                                                                                                 gCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcagca                      >  1:2170773/1‑150 (MQ=255)

                                                                                                                                 gCCATGACCGCGCATCCGGCAGACATTTCCTGGTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGgcagca                      <  2:2075225/150‑1 (MQ=255)

                                                                                                                                   cATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAAt                    >  2:1630177/1‑150 (MQ=255)

                                                                                                                                    aTGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATc                   <  1:2941849/150‑1 (MQ=255)

                                                                                                                                    aTGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATc                   <  1:2665570/150‑1 (MQ=255)

                                                                                                                                     tGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCg                  >  1:3646242/1‑150 (MQ=255)

                                                                                                                                        ccGCGCATCCGGCTGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccc               <  2:2924135/150‑1 (MQ=255)

                                                                                                                                        ccGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGccc               >  2:2693936/1‑150 (MQ=255)

                                                                                                                                           cgcATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCt            <  1:3239054/150‑1 (MQ=255)

                                                                                                                                           cgcATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCt            <  1:3580636/150‑1 (MQ=255)

                                                                                                                                           cgcATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCt            >  2:2435291/1‑150 (MQ=255)

                                                                                                                                            gcATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTg           <  1:2761456/150‑1 (MQ=255)

                                                                                                                                              aTCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGtt         <  2:4034997/150‑1 (MQ=255)

                                                                                                                                              aTCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGtt         <  2:4034714/150‑1 (MQ=255)

                                                                                                                                                  ggCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAg     <  2:15673/150‑1 (MQ=255)

                                                                                                                                                    cAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTa   >  2:2575622/1‑150 (MQ=255)

AGGTAGAGAAAACCGACAATTTTATCCTGCTCACGGCAACCGAATGCTTCACGCACTACCGGACTTTCAGTTAATGCGCCACTGCGCCAGATGCCGCCAAACCCCTGGGCAACTGCTGCCATTTGCATCGCCATGACCGCGCATCCGGCAGACATTTCCTGTTCCCAGCGCGGGACTTTATGATTCTCTTCGCATTTCGCCACCACCGTGATGATGAGCGGTGCGCGGAACGGCGCATTACGGGCTTTGTCGATAGCTTTGTCATCACTACCGGCAGCAATCGCCCCCTGTTCCAGTAC  >  CP009273/1842455‑1842753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`