Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,850,880 C→A 38.4% V102V (GTG→GTT)  ydjH ← putative kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,850,8800CA38.4% 345.2 / 275.4 255V102V (GTG→GTT) ydjHputative kinase
Reads supporting (aligned to +/- strand):  ref base C (81/76);  new base A (41/57);  total (122/133)
Fisher's exact test for biased strand distribution p-value = 1.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 257 total aligned reads displayed.

GCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAC  >  CP009273/1850731‑1851028
                                                                                                                                                     |                                                                                                                                                    
gcTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTc                                                                                                                                                      >  2:741766/1‑150 (MQ=255)
 cTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCa                                                                                                                                                     >  1:3402414/1‑150 (MQ=255)
  tttACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCAc                                                                                                                                                    >  1:3531469/1‑150 (MQ=255)
     aCCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAg                                                                                                                                                 >  1:1841656/1‑150 (MQ=255)
      ccATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGt                                                                                                                                                <  1:3071147/150‑1 (MQ=255)
        aTCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTcc                                                                                                                                              >  1:1311588/1‑150 (MQ=255)
        aTCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACAGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTcc                                                                                                                                              <  2:3126437/150‑1 (MQ=255)
          ccAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCaa                                                                                                                                            >  1:2484325/1‑150 (MQ=255)
            aaTAGTGGACTGTTGAAAATCCTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                                                                                                          >  2:2219180/1‑150 (MQ=255)
            aaTAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACg                                                                                                                                          >  2:3200528/1‑150 (MQ=255)
               aGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTa                                                                                                                                       >  2:2506197/1‑150 (MQ=255)
               aGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTa                                                                                                                                       >  2:718249/1‑150 (MQ=255)
                  ggACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATa                                                                                                                                    <  2:3693001/150‑1 (MQ=255)
                  ggACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATa                                                                                                                                    <  1:596484/150‑1 (MQ=255)
                   gACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATag                                                                                                                                   <  1:3462227/150‑1 (MQ=255)
                    aCTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATaga                                                                                                                                  >  2:145835/1‑150 (MQ=255)
                    aCTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATaga                                                                                                                                  >  1:1072591/1‑150 (MQ=255)
                     cTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATagag                                                                                                                                 >  2:426429/1‑150 (MQ=255)
                           aaaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCt                                                                                                                           <  1:1039482/150‑1 (MQ=255)
                           aaaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCAGTAACCAGTCCAACGTTAATAGAGGTATCt                                                                                                                           <  1:2139441/150‑1 (MQ=255)
                            aaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTa                                                                                                                          <  1:3797912/150‑1 (MQ=255)
                            aaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTa                                                                                                                          <  2:2379444/150‑1 (MQ=255)
                                aCTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCt                                                                                                                      <  1:471530/150‑1 (MQ=255)
                                 cTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTg                                                                                                                     >  1:98428/1‑150 (MQ=255)
                                  tGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGa                                                                                                                    <  2:718058/150‑1 (MQ=255)
                                   ggCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGAc                                                                                                                   >  2:2593976/1‑150 (MQ=255)
                                        gTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCt                                                                                                              <  2:1636097/150‑1 (MQ=255)
                                        gTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCt                                                                                                              <  2:2893017/150‑1 (MQ=255)
                                         tGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTg                                                                                                             <  2:1770688/150‑1 (MQ=255)
                                           ataataATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCt                                                                                                           >  1:3343169/1‑150 (MQ=255)
                                              ataatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCa                                                                                                        <  2:1067189/150‑1 (MQ=255)
                                                aatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAgg                                                                                                      <  2:3593137/150‑1 (MQ=255)
                                                  tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCt                                                                                                    <  2:2904032/150‑1 (MQ=255)
                                                  tttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCt                                                                                                    <  1:2063664/150‑1 (MQ=255)
                                                   ttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCtt                                                                                                   <  1:2898250/150‑1 (MQ=255)
                                                    tCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCttt                                                                                                  <  2:26220/150‑1 (MQ=255)
                                                    tCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATGTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCttt                                                                                                  <  2:3249417/150‑1 (MQ=255)
                                                            gaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCa                                                                                          <  1:1486154/150‑1 (MQ=255)
                                                            gaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCa                                                                                          >  1:1062162/1‑150 (MQ=255)
                                                             aaaaCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCaa                                                                                         >  1:1581852/1‑150 (MQ=255)
                                                               aaCCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAata                                                                                       >  1:688060/1‑150 (MQ=255)
                                                                acagcgcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAatat                                                                                      <  1:302094/147‑1 (MQ=255)
                                                                aCCGCGCAAAATCAACATCATCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAatat                                                                                      >  2:1119959/1‑150 (MQ=255)
                                                                     gcAAAATCAACATCGTCAATATTCAATATCCACAGACTGCCATTACGGTTAGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATATAGGTATCTATGCTGACGGCCTGATTCATGCTATGAATATCAATATActct                                                                                 >  2:281022/1‑150 (MQ=255)
                                                                             aaCATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                         >  1:3873104/1‑150 (MQ=255)
                                                                             aaCATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                         >  1:3872840/1‑150 (MQ=255)
                                                                             aaCATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCa                                                                         <  2:1824097/150‑1 (MQ=255)
                                                                               cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAAt                                                                       <  1:3282497/150‑1 (MQ=255)
                                                                               cATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAAt                                                                       <  1:3940154/150‑1 (MQ=255)
                                                                                aTCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATg                                                                      <  2:59699/150‑1 (MQ=255)
                                                                                  cGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCACTATTCTCTTTGCGGCAATGGt                                                                    <  2:2274580/150‑1 (MQ=255)
                                                                                  cGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGt                                                                    <  1:2724845/150‑1 (MQ=255)
                                                                                   gTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTc                                                                   >  2:3948833/1‑150 (MQ=255)
                                                                                        tatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGaa                                                              >  2:699811/1‑150 (MQ=255)
                                                                                        tatTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGaa                                                              >  2:1920521/1‑150 (MQ=255)
                                                                                        tatTCAATGTCCACAGACTGCCAGTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGaa                                                              <  2:1143329/150‑1 (MQ=255)
                                                                                         atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAAt                                                             >  1:1163578/1‑150 (MQ=255)
                                                                                         atTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAAt                                                             >  1:71533/1‑150 (MQ=255)
                                                                                             aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAt                                                         >  2:2956944/1‑150 (MQ=255)
                                                                                             aaTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAt                                                         <  2:3522435/150‑1 (MQ=255)
                                                                                              aTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAAtt                                                        <  1:4075774/150‑1 (MQ=255)
                                                                                               tttCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTg                                                       <  1:1207596/150‑1 (MQ=255)
                                                                                                  ccACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGccc                                                    >  1:334359/1‑150 (MQ=255)
                                                                                                   cacaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCt                                                   <  1:941552/150‑1 (MQ=255)
                                                                                                   cacaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCt                                                   <  1:1008263/150‑1 (MQ=255)
                                                                                                    acaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTg                                                  >  2:4150603/1‑150 (MQ=255)
                                                                                                    acaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTg                                                  <  2:1239303/150‑1 (MQ=255)
                                                                                                    acaGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTg                                                  <  1:1972316/150‑1 (MQ=255)
                                                                                                      aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                <  1:3243406/150‑1 (MQ=255)
                                                                                                      aGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                                >  2:2118011/1‑150 (MQ=255)
                                                                                                       gACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCgg                                               <  2:2763109/150‑1 (MQ=255)
                                                                                                         cTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCa                                             >  2:1011429/1‑150 (MQ=255)
                                                                                                          tGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                            <  1:4172956/150‑1 (MQ=255)
                                                                                                             cATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCtt                                         >  1:1152323/1‑150 (MQ=255)
                                                                                                              aTTACGGTTTGTGACAACCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                        <  2:3665630/150‑1 (MQ=255)
                                                                                                              aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                        <  2:3903791/150‑1 (MQ=255)
                                                                                                              aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                        >  2:4027599/1‑150 (MQ=255)
                                                                                                              aTTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCttt                                        <  1:623535/150‑1 (MQ=255)
                                                                                                               ttACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTa                                       >  1:2966638/1‑150 (MQ=255)
                                                                                                                  cGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCa                                    >  2:1619415/1‑150 (MQ=255)
                                                                                                                     tttGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATa                                 >  1:2839630/1‑150 (MQ=255)
                                                                                                                      ttGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATAc                                <  1:3518881/150‑1 (MQ=255)
                                                                                                                      ttGTGACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATAc                                <  2:1740361/150‑1 (MQ=255)
                                                                                                                       tgtgACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACg                               >  1:1611559/1‑150 (MQ=255)
                                                                                                                       tgtgACAAACGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACg                               <  1:2076604/150‑1 (MQ=255)
                                                                                                                             aaaCGTCCGCTCGCCATCCTCCGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAt                         >  2:1147552/1‑150 (MQ=255)
                                                                                                                              aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                        <  2:155041/150‑1 (MQ=255)
                                                                                                                              aaCGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCAtt                        >  1:2307217/1‑150 (MQ=255)
                                                                                                                                 gTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                     <  1:2742993/150‑1 (MQ=255)
                                                                                                                                 gTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAAt                     <  1:2666367/150‑1 (MQ=255)
                                                                                                                                     gCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtg                 >  2:1956876/1‑150 (MQ=255)
                                                                                                                                       tCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCtgtg               >  1:3743979/1‑150 (MQ=255)
                                                                                                                                        cGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGc              >  1:663011/1‑150 (MQ=255)
                                                                                                                                         gCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCg             <  2:3944382/150‑1 (MQ=255)
                                                                                                                                           cATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGAt           >  1:2269403/1‑150 (MQ=255)
                                                                                                                                              cctccGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGc        <  1:4043020/150‑1 (MQ=255)
                                                                                                                                               ctccGTAACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGcc       >  2:517906/1‑150 (MQ=255)
                                                                                                                                                tccGTCACCAGTCAAACGTTAATAGAGGTATCTATGATGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCATTGGTCTAGAATAAATTGCCCTGCGTCATCTTTACCAATACGAATCATTAATGCTGTGCGATGGccc      <  2:8443/150‑1 (MQ=255)
                                                                                                                                                    tCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAc  >  2:3320374/1‑150 (MQ=255)
                                                                                                                                                    tCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAc  <  1:3304981/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCTTTACCATCCAATAGTGGACTGTTGAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGATGGCCCAGAC  >  CP009273/1850731‑1851028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑