BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,942,066	G→T	31.5%	H115N (CAT→AAT)	yebC ←	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,942,066	0	G	T	31.5%	466.4 / 209.0	241	H115N (CAT→AAT)	yebC	UPF0082 family protein
Reads supporting (aligned to +/- strand): ref base G (75/90); new base T (35/41); total (110/131)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 244 total aligned reads displayed.

GGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTG  >  CP009273/1941917‑1942215

 gTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGa                                                                                                                                                      >  2:2064263/1‑150 (MQ=255)

   cacaACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACg                                                                                                                                                    <  1:3095014/150‑1 (MQ=255)

     caACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCa                                                                                                                                                  <  1:2542970/150‑1 (MQ=255)

           cTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAg                                                                                                                                            <  1:2389860/150‑1 (MQ=255)

           cTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAg                                                                                                                                            <  1:2294300/150‑1 (MQ=255)

             tCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCa                                                                                                                                          >  2:2255060/1‑150 (MQ=255)

              cAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCaa                                                                                                                                         >  1:1682440/1‑150 (MQ=255)

              cAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCaa                                                                                                                                         >  1:3780922/1‑150 (MQ=255)

               aGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAac                                                                                                                                        >  2:1032587/1‑150 (MQ=255)

                gCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacg                                                                                                                                       <  1:1202617/150‑1 (MQ=255)

                   ccTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAacggta                                                                                                                                    <  1:1073321/150‑1 (MQ=255)

                      gCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAacggtacgg                                                                                                                                 >  2:3627029/1‑150 (MQ=255)

                        ttCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTAcggtt                                                                                                                               <  1:1152244/150‑1 (MQ=255)

                         tCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTAcggttg                                                                                                                              <  2:1152662/150‑1 (MQ=255)

                         tCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTAcggttg                                                                                                                              >  1:170258/1‑150 (MQ=255)

                           cAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTAcggttgcg                                                                                                                            <  2:2947655/150‑1 (MQ=255)

                             gTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTAcggttgcggt                                                                                                                          <  1:1213401/150‑1 (MQ=255)

                              tgctgcTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTAcggttgcggtt                                                                                                                         >  1:2836686/1‑150 (MQ=255)

                                ctgctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGt                                                                                                                       >  2:138241/1‑150 (MQ=255)

                                 tgctTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTc                                                                                                                      <  1:763787/150‑1 (MQ=255)

                                           atgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACAt                                                                                                            <  1:108163/150‑1 (MQ=255)

                                           atgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACAt                                                                                                            <  1:155916/150‑1 (MQ=255)

                                            tgGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACAtt                                                                                                           >  1:2336981/1‑150 (MQ=255)

                                                  cTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCa                                                                                                     <  1:4102275/150‑1 (MQ=255)

                                                   ttCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCAt                                                                                                    <  2:2571305/150‑1 (MQ=255)

                                                    tCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATg                                                                                                   >  2:742395/1‑150 (MQ=255)

                                                      aTCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGAt                                                                                                 >  2:582023/1‑150 (MQ=255)

                                                         gCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgc                                                                                              >  1:2855408/1‑150 (MQ=255)

                                                           cTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccg                                                                                            >  2:3743684/1‑150 (MQ=255)

                                                           cTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccg                                                                                            <  1:3743003/150‑1 (MQ=255)

                                                             ttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtg                                                                                          >  1:166174/1‑150 (MQ=255)

                                                             ttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtg                                                                                          >  1:149602/1‑150 (MQ=255)

                                                             ttCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtg                                                                                          <  1:2163764/150‑1 (MQ=255)

                                                               ctcGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATtgccgtgcc                                                                                        >  2:2138143/1‑150 (MQ=255)

                                                                 cGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTgccgc                                                                                      <  1:3186881/150‑1 (MQ=255)

                                                                 cGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACGTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATAGCCGTgccgc                                                                                      >  2:2449741/1‑150 (MQ=255)

                                                                  gAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTgccgcc                                                                                     >  2:2528117/1‑150 (MQ=255)

                                                                   aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCa                                                                                    <  2:3307133/150‑1 (MQ=255)

                                                                   aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCa                                                                                    >  2:3106424/1‑150 (MQ=255)

                                                                   aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCa                                                                                    <  2:3497128/150‑1 (MQ=255)

                                                                   aaGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCa                                                                                    <  1:2605913/150‑1 (MQ=255)

                                                                    aGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAg                                                                                   <  2:1018648/150‑1 (MQ=255)

                                                                     ggAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAgg                                                                                  <  2:1245367/150‑1 (MQ=255)

                                                                       agaTCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGAc                                                                                >  1:1927423/1‑150 (MQ=255)

                                                                        gaTCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGAcc                                                                               >  1:3987873/1‑150 (MQ=255)

                                                                        gaTCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGAcc                                                                               >  1:2503619/1‑150 (MQ=255)

                                                                         aTCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCg                                                                              >  1:439568/1‑150 (MQ=255)

                                                                          tCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGCGCCGCCAGGACCGt                                                                             >  2:3280299/1‑150 (MQ=255)

                                                                           cACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTa                                                                            >  1:3176561/1‑150 (MQ=255)

                                                                                cTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCtt                                                                       >  1:3024575/1‑150 (MQ=255)

                                                                                 ttttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTc                                                                      <  1:3882038/150‑1 (MQ=255)

                                                                                 ttttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTc                                                                      <  1:507384/150‑1 (MQ=255)

                                                                                   ttttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGt                                                                    <  1:7862/150‑1 (MQ=255)

                                                                                    tttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTa                                                                   <  2:415777/150‑1 (MQ=255)

                                                                                    tttGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTa                                                                   <  2:2795100/150‑1 (MQ=255)

                                                                                      tGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAga                                                                 <  1:2712869/150‑1 (MQ=255)

                                                                                           aaCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGatgatg                                                            >  2:203894/1‑150 (MQ=255)

                                                                                           aaCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGatgatg                                                            <  2:656076/150‑1 (MQ=255)

                                                                                           aaCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGatgatg                                                            <  2:1968468/150‑1 (MQ=255)

                                                                                            aCAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATgg                                                           >  1:1407073/1‑150 (MQ=255)

                                                                                              aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         >  1:463803/1‑150 (MQ=255)

                                                                                              aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         <  2:1638898/150‑1 (MQ=255)

                                                                                              aGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGtt                                                         >  1:930804/1‑150 (MQ=255)

                                                                                                aTAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTc                                                       <  2:3077013/150‑1 (MQ=255)

                                                                                                   ggCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCAt                                                    <  2:3383376/150‑1 (MQ=255)

                                                                                                    gCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATg                                                   <  1:3260584/150‑1 (MQ=255)

                                                                                                    gCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATg                                                   <  2:2225966/150‑1 (MQ=255)

                                                                                                     cAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGt                                                  >  1:1570551/1‑150 (MQ=255)

                                                                                                     cAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGt                                                  >  1:191196/1‑150 (MQ=255)

                                                                                                      aaCGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGtt                                                 >  2:422461/1‑150 (MQ=255)

                                                                                                      aaCGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGtt                                                 >  2:3941826/1‑150 (MQ=255)

                                                                                                        cGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTg                                               >  2:40659/1‑150 (MQ=255)

                                                                                                        cGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTg                                               <  1:2261856/150‑1 (MQ=255)

                                                                                                         ggAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGc                                              >  1:3205652/1‑150 (MQ=255)

                                                                                                          gAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGca                                             >  1:1517693/1‑150 (MQ=255)

                                                                                                          gAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGca                                             >  1:3580246/1‑150 (MQ=255)

                                                                                                              cATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcat                                         <  2:3982943/150‑1 (MQ=255)

                                                                                                               ctCAGTACAGTGGTTTCCGCCACATTGGCTAAATGCATTACTCACTTCAGCAACGGTACGGTTGCTGTTGTCAGACTGACTTTCTTTCATGTTTGACGTGCCGCCAGGTCCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatc                                        <  1:2475351/149‑1 (MQ=255)

                                                                                                                 cAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGcatcatcat                                      <  2:689696/150‑1 (MQ=255)

                                                                                                                    tACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCAc                                   >  2:1941670/1‑150 (MQ=255)

                                                                                                                    tACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCAc                                   >  2:4018893/1‑150 (MQ=255)

                                                                                                                     aCCGAGGTTACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCAcc                                  >  2:4033773/1‑150 (MQ=255)

                                                                                                                         aGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGccc                              >  1:1006458/1‑150 (MQ=255)

                                                                                                                           gTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCcac                            <  2:2326231/150‑1 (MQ=255)

                                                                                                                             tACCGCCACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCacac                          >  2:2584686/1‑150 (MQ=255)

                                                                                                                             tACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCacac                          <  2:230828/150‑1 (MQ=255)

                                                                                                                                cGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCAcaccac                       <  1:1710670/150‑1 (MQ=255)

                                                                                                                                  ccACATTTGCTAAATGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGt                     >  2:2474058/1‑150 (MQ=255)

                                                                                                                                   cacaTTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTg                    >  1:3145260/1‑150 (MQ=255)

                                                                                                                                    acaTTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGc                   >  2:2841148/1‑150 (MQ=255)

                                                                                                                                      aTTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCaa                 >  1:3848632/1‑150 (MQ=255)

                                                                                                                                         tGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTg              <  2:1623232/150‑1 (MQ=255)

                                                                                                                                             aaaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGcgcg          <  1:2064263/150‑1 (MQ=255)

                                                                                                                                             aaaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGcgcg          >  1:3361982/1‑150 (MQ=255)

                                                                                                                                             aaaTGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGcgcg          >  1:3362008/1‑150 (MQ=255)

                                                                                                                                                tGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGtt       <  1:1053676/150‑1 (MQ=255)

                                                                                                                                                tGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGtt       >  2:181865/1‑150 (MQ=255)

                                                                                                                                                tGCATTACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGtt       <  2:1799974/150‑1 (MQ=255)

                                                                                                                                                 gCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTc      <  1:1852964/150‑1 (MQ=255)

                                                                                                                                                 gCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTc      <  1:3938919/150‑1 (MQ=255)

GGTCACAACGTCTTCAGCACCTGCTTCCAGTGCTGCTTCCATGATGGTGTCTTCATCGCCTTTCTCGAAGGAGATCACGCCTTTTTTGCTGAACAGATAGGCAACGGAACCATCAGTACCGAGGTTACCGCCACATTTGCTAAATGCATGACGCACTTCAGCAACGGTACGGTTGCGGTTGTCAGACAGACATTCAATCATGATTGCCGTGCCGCCAGGACCGTAACCTTCGTAGATGATGGTTTCCATGTTTGCATCATCATCACCGCCCACACCACGTGCAATTGCGCGGTTCAGTG  >  CP009273/1941917‑1942215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`