Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,943,414 C→A 5.2% D456Y (GAT→TAT)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,943,4140CA 5.2% 902.9 / 17.8 231D456Y (GAT→TAT) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (112/107);  new base A (3/9);  total (115/116)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 237 total aligned reads displayed.

GATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCA  >  CP009273/1943265‑1943563
                                                                                                                                                     |                                                                                                                                                     
gATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACAAATGATGACAATATCTAAAGAGTTCGACACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCTTCATATc                                                                                                                                                       >  1:4148905/1‑150 (MQ=255)
 aTACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCt                                                                                                                                                      >  1:544036/1‑150 (MQ=255)
 aTACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCt                                                                                                                                                      <  1:3898273/150‑1 (MQ=255)
   aCCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCttt                                                                                                                                                    <  1:3044485/150‑1 (MQ=255)
   aCCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCttt                                                                                                                                                    >  1:2715458/1‑150 (MQ=255)
     ccAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCg                                                                                                                                                  >  1:507420/1‑150 (MQ=255)
     ccAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACAACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTATGCAGCCGTCAAATCTTTCg                                                                                                                                                  >  2:119721/1‑150 (MQ=255)
        gAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtg                                                                                                                                               >  2:3151686/1‑150 (MQ=255)
         aaTACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtga                                                                                                                                              <  1:3576356/150‑1 (MQ=255)
         aaTACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtga                                                                                                                                              <  1:4015538/150‑1 (MQ=255)
          aTACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgag                                                                                                                                             <  2:4051549/150‑1 (MQ=255)
           tACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgagg                                                                                                                                            >  2:437933/1‑150 (MQ=255)
           tACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCggtgagg                                                                                                                                            >  2:109958/1‑150 (MQ=255)
                aaCACCTTCTTCTGCATATCACCATTATGGATACTTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGAGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAAc                                                                                                                                       <  1:3156081/150‑1 (MQ=255)
                aaCACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAAc                                                                                                                                       >  2:3476422/1‑150 (MQ=255)
                 acacCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACg                                                                                                                                      <  1:4163405/150‑1 (MQ=255)
                  cacCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACgg                                                                                                                                     >  1:3791153/1‑150 (MQ=255)
                  cacCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACgg                                                                                                                                     >  1:943539/1‑150 (MQ=255)
                    ccGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGAt                                                                                                                                   <  2:669859/150‑1 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATg                                                                                                                                  >  1:3975159/1‑150 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATg                                                                                                                                  >  1:2811594/1‑150 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATg                                                                                                                                  >  2:601440/1‑150 (MQ=255)
                     cGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATg                                                                                                                                  <  1:4051820/150‑1 (MQ=255)
                      gTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATgg                                                                                                                                 <  2:2644616/150‑1 (MQ=255)
                      gTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATgg                                                                                                                                 >  2:3788015/1‑150 (MQ=255)
                      gTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATgg                                                                                                                                 >  2:1689111/1‑150 (MQ=255)
                      gTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATgg                                                                                                                                 >  1:2043335/1‑150 (MQ=255)
                        ctgctgCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTg                                                                                                                               <  1:3517676/150‑1 (MQ=255)
                        ctgctgCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTg                                                                                                                               <  1:1105373/150‑1 (MQ=255)
                          gctgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCa                                                                                                                             >  1:564849/1‑150 (MQ=255)
                           ctgcATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCAt                                                                                                                            <  1:2902734/150‑1 (MQ=255)
                              cATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGc                                                                                                                         <  2:1430551/150‑1 (MQ=255)
                                tatCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCg                                                                                                                       >  1:1917800/1‑150 (MQ=255)
                                 atCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGt                                                                                                                      >  2:4112219/1‑150 (MQ=255)
                                  tCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTc                                                                                                                     >  1:982074/1‑150 (MQ=255)
                                     ccATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAgg                                                                                                                  >  1:311667/1‑150 (MQ=255)
                                     ccATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAgg                                                                                                                  >  2:3275911/1‑150 (MQ=255)
                                          aTGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGgccgcc                                                                                                             >  1:317187/1‑150 (MQ=255)
                                               tACGTACTGAATCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAc                                                                                                        >  1:1076554/1‑150 (MQ=255)
                                               tACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTGTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCAc                                                                                                        >  2:255020/1‑150 (MQ=255)
                                                 cGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACcg                                                                                                      >  1:2800721/1‑150 (MQ=255)
                                                 cGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACcg                                                                                                      >  2:2560452/1‑150 (MQ=255)
                                                   tACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACcgtc                                                                                                    <  2:3986447/150‑1 (MQ=255)
                                                    aCTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACcgtcg                                                                                                   >  1:4157522/1‑150 (MQ=255)
                                                       gAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCt                                                                                                >  1:2146684/1‑150 (MQ=255)
                                                        aaCCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGACCGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCtt                                                                                               >  2:895192/1‑150 (MQ=255)
                                                          ccaccGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCa                                                                                             >  1:270488/1‑150 (MQ=255)
                                                           caccGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCaa                                                                                            >  2:2911967/1‑150 (MQ=255)
                                                              cGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACa                                                                                         >  1:4111014/1‑150 (MQ=255)
                                                              cGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACa                                                                                         >  1:2591412/1‑150 (MQ=255)
                                                                  cACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCgg                                                                                     >  1:3307747/1‑150 (MQ=255)
                                                                  cACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCgg                                                                                     <  2:2299327/150‑1 (MQ=255)
                                                                    cTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGa                                                                                   >  1:253926/1‑150 (MQ=255)
                                                                         tAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCg                                                                              <  1:3231379/150‑1 (MQ=255)
                                                                         tAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCg                                                                              <  1:268184/150‑1 (MQ=255)
                                                                           aCCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGAt                                                                            <  2:3076646/150‑1 (MQ=255)
                                                                             cATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATaa                                                                          <  2:1291606/150‑1 (MQ=255)
                                                                               ttGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAAcc                                                                        <  1:2292119/150‑1 (MQ=255)
                                                                                 gatgaCCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCca                                                                      >  1:1546095/1‑150 (MQ=255)
                                                                                     aCCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGc                                                                  <  1:1306273/150‑1 (MQ=255)
                                                                                      ccATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCg                                                                 <  2:215570/150‑1 (MQ=255)
                                                                                              tAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAt                                                         >  2:1959062/1‑150 (MQ=255)
                                                                                              tAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGACTTCAAACATCGGGAAGTCGAAAACCCACAGCGGTGCCCAt                                                         >  2:3235618/1‑150 (MQ=255)
                                                                                               aaGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAtt                                                        <  1:1830136/150‑1 (MQ=255)
                                                                                                aGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAttt                                                       >  1:1412228/1‑150 (MQ=255)
                                                                                                aGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAttt                                                       >  2:695810/1‑150 (MQ=255)
                                                                                                 gCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTg                                                      <  1:3711716/150‑1 (MQ=255)
                                                                                                  cGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGc                                                     >  1:3037023/1‑150 (MQ=255)
                                                                                                   gTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCt                                                    <  2:2017555/150‑1 (MQ=255)
                                                                                                      cGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtc                                                 <  1:1560400/150‑1 (MQ=255)
                                                                                                      cGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGAAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTtc                                                 <  1:1080931/150‑1 (MQ=255)
                                                                                                             gCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTc                                          <  1:2834619/150‑1 (MQ=255)
                                                                                                             gCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTc                                          >  2:797452/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCa                                         >  1:3424287/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCa                                         >  1:699876/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCa                                         >  2:1075058/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCAGTTGCTTTCGTCGGTCa                                         >  2:3862078/1‑150 (MQ=255)
                                                                                                              cATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCa                                         <  1:1752914/150‑1 (MQ=255)
                                                                                                               aTTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAg                                        >  1:1134071/1‑150 (MQ=255)
                                                                                                                ttttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGa                                       >  1:2438050/1‑150 (MQ=255)
                                                                                                                ttttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGa                                       >  1:783938/1‑150 (MQ=255)
                                                                                                                 tttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGAc                                      <  1:2015870/150‑1 (MQ=255)
                                                                                                                 tttCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGAc                                      >  2:3552598/1‑150 (MQ=255)
                                                                                                                      ggTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAgg                                 >  2:1983422/1‑150 (MQ=255)
                                                                                                                      ggTGCAGCTTTCAGTTCTGCAGCCGTCATATATTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAgg                                 <  1:28952/150‑1 (MQ=255)
                                                                                                                       gTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGt                                <  1:3151686/150‑1 (MQ=255)
                                                                                                                           aGCTTTCAGTTCTGCAGCCGTCAGATATTTCGGTGAGGTGANCGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGNTCAGACCAAGGTCttt                            <  2:2093584/150‑1 (MQ=255)
                                                                                                                             cTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAc                          >  2:3150908/1‑150 (MQ=255)
                                                                                                                               ttCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTAccc                        >  2:3051994/1‑150 (MQ=255)
                                                                                                                                tCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCa                       <  1:1239134/150‑1 (MQ=255)
                                                                                                                                   gTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACtt                    <  2:4144305/150‑1 (MQ=255)
                                                                                                                                    ttCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACttt                   <  2:3437133/150‑1 (MQ=255)
                                                                                                                                     tCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTc                  <  2:339259/150‑1 (MQ=255)
                                                                                                                                       tGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAg                <  1:1595332/150‑1 (MQ=255)
                                                                                                                                         cAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGgc              <  1:687180/150‑1 (MQ=255)
                                                                                                                                              gTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGt         >  1:3197047/1‑150 (MQ=255)
                                                                                                                                              gTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGt         <  1:1998687/150‑1 (MQ=255)
                                                                                                                                                cATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGc       <  1:1202132/150‑1 (MQ=255)
                                                                                                                                                cATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGc       <  2:564849/150‑1 (MQ=255)
                                                                                                                                                     cTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCa  >  2:2886384/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATACCCAGAATACCAAACACCGTCTGCTGCATATCACCATTATGGATACGTACTGAACCACCGCCCACTTCGTAACCATTGATGACCATATCGTAAGCGTTCGCCACCGCATTTTCCGGTGCAGCTTTCAGTTCTGCAGCCGTCATATCTTTCGGTGAGGTGAACGGATGGTGCATTGCCGTCAGGCCGCCTTCACCGTCGTCTTCAAACATCGGGAAGTCGATAACCCACAGCGGTGCCCATTTGCTTTCGTCGGTCAGACCAAGGTCTTTACCCACTTTCAGGCGCAGTGCACCCA  >  CP009273/1943265‑1943563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑