Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,944,177 C→A 100% L201L (CTG→CTT)  aspS ← aspartyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,944,1770CA99.6% 953.6 / ‑4.1 265L201L (CTG→CTT) aspSaspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (119/145);  total (119/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 276 total aligned reads displayed.

GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944031‑1944323
                                                                                                                                                  |                                                                                                                                                  
   tCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAagcagc                                                                                                                                              <  2:1322347/150‑1 (MQ=255)
   tCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAagcagc                                                                                                                                              >  1:3718960/1‑150 (MQ=255)
    ccATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCt                                                                                                                                             >  2:2966979/1‑150 (MQ=255)
    ccATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCt                                                                                                                                             <  1:907517/150‑1 (MQ=255)
       tCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGtt                                                                                                                                          <  1:775271/150‑1 (MQ=255)
          cTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGa                                                                                                                                       <  2:2720464/150‑1 (MQ=255)
                gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACt                                                                                                                                 <  2:1258784/150‑1 (MQ=255)
                gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACt                                                                                                                                 <  2:1202206/150‑1 (MQ=255)
                gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACt                                                                                                                                 <  2:2467276/150‑1 (MQ=255)
                gCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATATGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACt                                                                                                                                 <  2:3113348/150‑1 (MQ=255)
                 cACGTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACNGGACATCATAAGCAGCTGTTTGAACAACTg                                                                                                                                <  1:3858052/150‑1 (MQ=255)
                       cggcgCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGa                                                                                                                          <  1:1599840/150‑1 (MQ=255)
                          cgcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTg                                                                                                                       <  1:2096302/150‑1 (MQ=255)
                          cgcgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTg                                                                                                                       <  1:1298656/150‑1 (MQ=255)
                            cgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCg                                                                                                                     >  2:872041/1‑150 (MQ=255)
                            cgGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCg                                                                                                                     >  2:2056660/1‑150 (MQ=255)
                             ggTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCgg                                                                                                                    >  1:61745/1‑150 (MQ=255)
                             ggTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCgg                                                                                                                    >  1:2560062/1‑150 (MQ=255)
                              gTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGc                                                                                                                   <  2:3140216/150‑1 (MQ=255)
                               tCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCa                                                                                                                  <  1:2508196/150‑1 (MQ=255)
                                  tGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTg                                                                                                               <  2:917442/150‑1 (MQ=255)
                                        aagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGTTCAGCACGCAGTTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGTTCAAAACCGGACATAATAAGCAGCTGTTTGAACAACTGAGGGGATTGCGGCAGTGCGTAGa                                                                                                         >  1:949341/1‑150 (MQ=255)
                                        aagTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGa                                                                                                         >  2:3502100/1‑150 (MQ=255)
                                         agTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                        >  2:2760706/1‑150 (MQ=255)
                                         agTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGaa                                                                                                        <  1:748457/150‑1 (MQ=255)
                                          gTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAAt                                                                                                       <  1:1492737/150‑1 (MQ=255)
                                                acaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCt                                                                                                 >  2:2989373/1‑150 (MQ=255)
                                                acaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCt                                                                                                 <  1:3591714/150‑1 (MQ=255)
                                                acaTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCt                                                                                                 <  1:1440642/150‑1 (MQ=255)
                                                 caTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAACCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCtt                                                                                                <  2:4177090/150‑1 (MQ=255)
                                                  aTCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCttt                                                                                               <  1:3199759/150‑1 (MQ=255)
                                                   tCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtg                                                                                              <  1:3049960/150‑1 (MQ=255)
                                                    cGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgt                                                                                             <  2:4168079/150‑1 (MQ=255)
                                                     gATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                            <  2:3960768/150‑1 (MQ=255)
                                                     gATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTtgtg                                                                                            >  2:1899770/1‑150 (MQ=255)
                                                      aTCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGc                                                                                           >  1:1787209/1‑150 (MQ=255)
                                                      aTCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGc                                                                                           >  1:3454623/1‑150 (MQ=255)
                                                       tCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGca                                                                                          >  1:1186719/1‑150 (MQ=255)
                                                        cTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGcac                                                                                         <  1:2448465/150‑1 (MQ=255)
                                                           aGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACg                                                                                      >  2:3356308/1‑150 (MQ=255)
                                                           aGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACg                                                                                      >  2:1113791/1‑150 (MQ=255)
                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                     >  1:3655519/1‑150 (MQ=255)
                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                     >  1:826966/1‑150 (MQ=255)
                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                     >  1:3429421/1‑150 (MQ=255)
                                                            gTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACga                                                                                     >  2:52663/1‑150 (MQ=255)
                                                              aaaTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACgaga                                                                                   >  2:2450368/1‑150 (MQ=255)
                                                              aaaTTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACgaga                                                                                   <  2:3919531/150‑1 (MQ=255)
                                                                 ttCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAgg                                                                                <  1:2001204/150‑1 (MQ=255)
                                                                   cAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCa                                                                              >  1:1096825/1‑150 (MQ=255)
                                                                    aGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCAc                                                                             >  1:1675761/1‑150 (MQ=255)
                                                                        tGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAgg                                                                         <  2:3842148/150‑1 (MQ=255)
                                                                             gTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTc                                                                    >  1:4037634/1‑150 (MQ=255)
                                                                              tCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCa                                                                   <  2:1832649/150‑1 (MQ=255)
                                                                                 gCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcgc                                                                >  1:1890502/1‑150 (MQ=255)
                                                                                  cACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcgcg                                                               <  1:2860067/150‑1 (MQ=255)
                                                                                    cGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCAcgcgcg                                                             <  1:3837161/150‑1 (MQ=255)
                                                                                        ggTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCtt                                                         <  2:3022375/150‑1 (MQ=255)
                                                                                        ggTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCtt                                                         >  2:724949/1‑150 (MQ=255)
                                                                                          tCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTcc                                                       <  2:656711/150‑1 (MQ=255)
                                                                                            ttCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCgg                                                     >  2:3183096/1‑150 (MQ=255)
                                                                                              cGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCg                                                   >  1:3473383/1‑150 (MQ=255)
                                                                                               gTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                  <  2:1179044/150‑1 (MQ=255)
                                                                                               gTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                  >  1:2665007/1‑150 (MQ=255)
                                                                                               gTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                  <  1:122258/150‑1 (MQ=255)
                                                                                               gTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                  >  1:3670935/1‑150 (MQ=255)
                                                                                               gTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGt                                                  >  1:2267070/1‑150 (MQ=255)
                                                                                                tCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTg                                                 <  1:3622395/150‑1 (MQ=255)
                                                                                                  aCGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGc                                               >  1:998301/1‑150 (MQ=255)
                                                                                                    ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATACGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCtt                                             <  1:449756/150‑1 (MQ=255)
                                                                                                    ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCtt                                             >  1:695735/1‑150 (MQ=255)
                                                                                                    ggAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCtt                                             <  2:2217241/150‑1 (MQ=255)
                                                                                                     gAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCttt                                            >  1:4129900/1‑150 (MQ=255)
                                                                                                         cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTc                                        <  2:3268295/150‑1 (MQ=255)
                                                                                                         cATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTc                                        >  2:1470178/1‑150 (MQ=255)
                                                                                                           tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGTGATTGCTGCAGTGCTTAGAATTTACCTTAGTGCACACGAGAAGGCACCAGGTAGTAACGCGCGCCTTCCTGCGAGGCATAGTTCAg                                      >  2:3658107/1‑150 (MQ=255)
                                                                                                           tttAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAg                                      <  2:1512086/150‑1 (MQ=255)
                                                                                                             tAACGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCa                                    >  2:4026089/1‑150 (MQ=255)
                                                                                                              aaCGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCAt                                   >  2:2788113/1‑150 (MQ=255)
                                                                                                              aaCGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCAt                                   <  1:2035441/150‑1 (MQ=255)
                                                                                                                cGATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCg                                 <  2:1625884/150‑1 (MQ=255)
                                                                                                                 gATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                >  2:243862/1‑150 (MQ=255)
                                                                                                                 gATCTGATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCgg                                >  2:3776755/1‑150 (MQ=255)
                                                                                                                      gATAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGttt                           >  1:2151197/1‑150 (MQ=255)
                                                                                                                       aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGTGTTTc                          <  2:3165680/150‑1 (MQ=255)
                                                                                                                       aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                          >  2:926639/1‑150 (MQ=255)
                                                                                                                       aTAGTAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTc                          <  2:1896951/150‑1 (MQ=255)
                                                                                                                           tAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATg                      >  2:3802125/1‑150 (MQ=255)
                                                                                                                           tAACGGTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATg                      <  1:2387286/150‑1 (MQ=255)
                                                                                                                               ggTCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGa                  >  1:2794321/1‑150 (MQ=255)
                                                                                                                                 tCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg                >  2:2557966/1‑150 (MQ=255)
                                                                                                                                 tCAAAACCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAgg                <  1:2833232/150‑1 (MQ=255)
                                                                                                                                   aaaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGaa              <  1:204880/150‑1 (MQ=255)
                                                                                                                                    aaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg             >  2:1576915/1‑150 (MQ=255)
                                                                                                                                    aaaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAg             <  1:762460/150‑1 (MQ=255)
                                                                                                                                     aaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc            <  2:3655519/150‑1 (MQ=255)
                                                                                                                                     aaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc            <  2:758074/150‑1 (MQ=255)
                                                                                                                                     aaCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGc            >  1:1355739/1‑150 (MQ=255)
                                                                                                                                      aCCGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGcc           <  1:2515159/150‑1 (MQ=255)
                                                                                                                                       ccGGACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCg          >  1:1758478/1‑150 (MQ=255)
                                                                                                                                         ggACATCATAAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTg        <  1:970319/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GCTTCCATCACTTCACGCACTTGCGGCGCGGTCATGAAAGAAGTTTCCACATCGATCTGAGTAAATTCAGGCTGACGGTCAGCACGCAGGTCTTCGTCACGGAAGCATTTAACGATCTGATAGTAACGGTCAAAACCGGACATCATCAGCAGCTGTTTGAACAACTGCGGGGATTGCGGCAGTGCGTAGAATTTACCTTTGTGCACACGAGAAGGCACCAGGTAGTCACGCGCGCCTTCCGGCGTGGCTTTGGTCAGCATCGGAGTTTCGATGTCGAGGAAGCCGTGGTCATC  >  CP009273/1944031‑1944323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑