Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,946,006 G→T 49.2% D119Y (GAT→TAT)  yecE → UPF0759 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,946,0060GT49.2% 89.9 / 286.8 195D119Y (GAT→TAT) yecEUPF0759 family protein
Reads supporting (aligned to +/- strand):  ref base G (57/42);  new base T (44/52);  total (101/94)
Fisher's exact test for biased strand distribution p-value = 1.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 196 total aligned reads displayed.

GCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAG  >  CP009273/1945858‑1946153
                                                                                                                                                    |                                                                                                                                                   
gCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGa                                                                                                                                                    >  2:2776596/1‑150 (MQ=255)
 cATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGAt                                                                                                                                                   <  2:1473240/150‑1 (MQ=255)
  aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTAtt                                                                                                                                                  >  1:1033921/1‑150 (MQ=255)
  aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTAtt                                                                                                                                                  >  2:2899641/1‑150 (MQ=255)
  aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTAtt                                                                                                                                                  <  2:418259/150‑1 (MQ=255)
  aTCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGAtt                                                                                                                                                  >  2:2743468/1‑150 (MQ=255)
   tCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATtc                                                                                                                                                 >  1:1126184/1‑150 (MQ=255)
   tCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATtc                                                                                                                                                 <  2:2326220/150‑1 (MQ=255)
    cAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATtct                                                                                                                                                <  2:727593/150‑1 (MQ=255)
    cAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATtct                                                                                                                                                <  1:3773428/150‑1 (MQ=255)
         agcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTcc                                                                                                                                           <  2:211329/150‑1 (MQ=255)
          gcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCCCCGTTGGCTACGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACCTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGTTTCTCTTCCt                                                                                                                                          <  1:315022/150‑1 (MQ=255)
          gcaTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCt                                                                                                                                          <  2:834547/150‑1 (MQ=255)
           caTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTg                                                                                                                                         >  1:2479537/1‑150 (MQ=255)
           caTTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTg                                                                                                                                         >  2:3818004/1‑150 (MQ=255)
             ttACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGc                                                                                                                                       >  1:1008246/1‑150 (MQ=255)
               aCGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGa                                                                                                                                     <  1:2833206/150‑1 (MQ=255)
                 ggCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAAt                                                                                                                                   <  1:3710429/150‑1 (MQ=255)
                    aTTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTa                                                                                                                                >  1:4103331/1‑150 (MQ=255)
                    aTTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTa                                                                                                                                >  2:2018844/1‑150 (MQ=255)
                    aTTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTa                                                                                                                                >  1:2527062/1‑150 (MQ=255)
                     ttGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTaa                                                                                                                               <  1:1741347/150‑1 (MQ=255)
                       gCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAAtt                                                                                                                             <  2:2552474/150‑1 (MQ=255)
                       gCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAAtt                                                                                                                             <  2:1789824/150‑1 (MQ=255)
                       gCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAAtt                                                                                                                             <  1:2657954/150‑1 (MQ=255)
                        cGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCTCGCATTGGTCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTa                                                                                                                            <  2:2522973/150‑1 (MQ=255)
                         gatgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTAt                                                                                                                           <  1:1270509/150‑1 (MQ=255)
                         gatgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTAt                                                                                                                           <  2:2727363/150‑1 (MQ=255)
                         gatgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACAGTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTAt                                                                                                                           <  2:2727334/150‑1 (MQ=255)
                          atgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATg                                                                                                                          <  2:3592931/150‑1 (MQ=255)
                           tgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATgg                                                                                                                         <  1:2890030/150‑1 (MQ=255)
                           tgatTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATgg                                                                                                                         >  1:776385/1‑150 (MQ=255)
                              tttAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGt                                                                                                                      >  1:2107869/1‑150 (MQ=255)
                              tttAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGt                                                                                                                      >  2:1996484/1‑150 (MQ=255)
                                 aGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGa                                                                                                                   >  1:2565416/1‑150 (MQ=255)
                                     aCTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTc                                                                                                               <  1:1518302/150‑1 (MQ=255)
                                       tGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCg                                                                                                             <  2:1706955/150‑1 (MQ=255)
                                       tGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCg                                                                                                             >  2:3777459/1‑150 (MQ=255)
                                         aaTTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGcc                                                                                                           <  2:3706361/150‑1 (MQ=255)
                                         aaTTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGcc                                                                                                           <  2:3706343/150‑1 (MQ=255)
                                           tttttGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCAt                                                                                                         >  2:4115342/1‑150 (MQ=255)
                                             tttGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATcc                                                                                                       <  1:1391489/150‑1 (MQ=255)
                                                  cccGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGt                                                                                                  <  2:1165817/150‑1 (MQ=255)
                                                    cGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttt                                                                                                >  1:2261569/1‑150 (MQ=255)
                                                    cGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGttt                                                                                                >  2:3061656/1‑150 (MQ=255)
                                                     gCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGtttt                                                                                               >  1:3209260/1‑150 (MQ=255)
                                                        tGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCg                                                                                            >  2:86696/1‑150 (MQ=255)
                                                          tCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGcc                                                                                          >  1:258181/1‑150 (MQ=255)
                                                              cGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAg                                                                                      <  1:2702267/150‑1 (MQ=255)
                                                              cGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAg                                                                                      >  2:4167191/1‑150 (MQ=255)
                                                               gTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAgg                                                                                     <  1:2394112/150‑1 (MQ=255)
                                                               gTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAgg                                                                                     <  1:3531274/150‑1 (MQ=255)
                                                                ttGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGga                                                                                    >  1:3892020/1‑150 (MQ=255)
                                                                ttGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGga                                                                                    >  2:2629654/1‑150 (MQ=255)
                                                                  ggCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGgaga                                                                                  >  2:3865680/1‑150 (MQ=255)
                                                                  ggCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGACGTCCGCCATCCACAGTTTTTCGCCAAAGgaga                                                                                  >  2:899381/1‑150 (MQ=255)
                                                                     tCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGagaaga                                                                               >  1:2208736/1‑150 (MQ=255)
                                                                      ccGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAagag                                                                              <  2:2208130/150‑1 (MQ=255)
                                                                             ttGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACaaa                                                                       <  2:2574451/150‑1 (MQ=255)
                                                                              tGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAAc                                                                      <  2:1034957/150‑1 (MQ=255)
                                                                               gggCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACg                                                                     >  1:3360693/1‑150 (MQ=255)
                                                                                   aaTACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTa                                                                 >  2:2332829/1‑150 (MQ=255)
                                                                                     tACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAAt                                                               <  1:2756671/150‑1 (MQ=255)
                                                                                     tACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAAt                                                               >  2:3240978/1‑150 (MQ=255)
                                                                                       cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATcg                                                             <  1:859872/150‑1 (MQ=255)
                                                                                       cTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATcg                                                             >  1:3634739/1‑150 (MQ=255)
                                                                                              cAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTa                                                      <  1:3247/150‑1 (MQ=255)
                                                                                              cAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTa                                                      <  1:2189660/150‑1 (MQ=255)
                                                                                                 ctgcctgcCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACAt                                                   <  1:1798809/150‑1 (MQ=255)
                                                                                                 ctgcctgcCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACAt                                                   <  1:2964363/150‑1 (MQ=255)
                                                                                                  tgcctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATc                                                  <  2:545779/150‑1 (MQ=255)
                                                                                                   gcctgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCa                                                 >  1:637815/1‑150 (MQ=255)
                                                                                                      tgccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAgcg                                              <  1:52918/150‑1 (MQ=255)
                                                                                                        ccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGcgcg                                            >  1:1546880/1‑150 (MQ=255)
                                                                                                        ccACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGcgcg                                            >  1:3401264/1‑150 (MQ=255)
                                                                                                         cacaTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCgcgg                                           >  1:2159514/1‑150 (MQ=255)
                                                                                                         cacaTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCgcgg                                           >  1:918292/1‑150 (MQ=255)
                                                                                                         cacaTTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCgcgg                                           <  1:558187/150‑1 (MQ=255)
                                                                                                                    cACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCggg                                >  2:4145751/1‑150 (MQ=255)
                                                                                                                     aCGGGAGCTGCCTGCGCTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGt                               <  1:506356/150‑1 (MQ=255)
                                                                                                                      cGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTg                              >  1:1476044/1‑150 (MQ=255)
                                                                                                                      cGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTg                              >  1:3022351/1‑150 (MQ=255)
                                                                                                                         gAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAtt                           >  1:1247667/1‑150 (MQ=255)
                                                                                                                          aGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAttt                          >  1:2777850/1‑150 (MQ=255)
                                                                                                                          aGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAttt                          >  2:15628/1‑150 (MQ=255)
                                                                                                                          aGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGAttt                          <  2:1351142/150‑1 (MQ=255)
                                                                                                                                ctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACa                    >  2:965964/1‑150 (MQ=255)
                                                                                                                                ctgcGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACa                    >  2:3363503/1‑150 (MQ=255)
                                                                                                                                   cgcTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgcc                 >  1:1594171/1‑150 (MQ=255)
                                                                                                                                   cgcTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgcc                 <  2:2397972/150‑1 (MQ=255)
                                                                                                                                     cTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgccgc               >  1:3432698/1‑150 (MQ=255)
                                                                                                                                     cTTTGGCATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAgccgc               >  1:1339533/1‑150 (MQ=255)
                                                                                                                                        tGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCg            >  2:1231614/1‑150 (MQ=255)
                                                                                                                                          gCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGt          <  1:1012088/150‑1 (MQ=255)
                                                                                                                                           cATTTTCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGtt         >  1:4093729/1‑150 (MQ=255)
                                                                                                                                            aTTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTc        >  2:2463337/1‑150 (MQ=255)
                                                                                                                                            aTTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTc        >  2:73597/1‑150 (MQ=255)
                                                                                                                                             ttttCTCTATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCa       >  1:3012280/1‑150 (MQ=255)
                                                                                                                                                 ctctATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATgca   <  1:1991963/150‑1 (MQ=255)
                                                                                                                                                  tcGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATgcag  >  1:1650396/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GCATCAGGCAGCATTACGGCATTGCGATGATTTAGTGACTGAATTTTTGACCCGCATGTCACCGTTGGCTCCGCGCATTGGGCAATACTGGCTGCAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCGCTTTGGCATTTTCTCGATTCTCTTCCTGGCGAATTTAATTATGGCGTGGAAGTCCGCCATCCACAGTTTTTCGCCAAAGGAGAAGAGGAACAAACGCTTAATCGCGGTTTACATCAGCGCGGCGTTAATCGGGTGATTTTAGACAGCCGCCCGGTTCATGCAG  >  CP009273/1945858‑1946153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑