Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,984,987 C→A 55.1% T38T (ACG→ACT)  yecA ← UPF0149 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,984,9870CA55.1% ‑4.5 / inf 218T38T (ACG→ACT) yecAUPF0149 family protein
Reads supporting (aligned to +/- strand):  ref base C (44/54);  new base A (60/60);  total (104/114)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

GCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984841‑1985136
                                                                                                                                                  |                                                                                                                                                     
gCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTc                                                                                                                                                    <  1:542499/150‑1 (MQ=255)
gCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTc                                                                                                                                                    <  1:2755360/150‑1 (MQ=255)
    tATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATa                                                                                                                                                <  1:3868090/150‑1 (MQ=255)
     aTCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATaa                                                                                                                                               <  2:3809957/150‑1 (MQ=255)
        ggCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAAcc                                                                                                                                            <  2:3641627/150‑1 (MQ=255)
         gCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCg                                                                                                                                           >  1:166195/1‑150 (MQ=255)
         gCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCg                                                                                                                                           >  2:3983403/1‑150 (MQ=255)
         gCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCg                                                                                                                                           >  1:638782/1‑150 (MQ=255)
          ccATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGt                                                                                                                                          <  1:2118583/150‑1 (MQ=255)
           cATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTc                                                                                                                                         <  2:530997/150‑1 (MQ=255)
            atatGTTGATAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTcc                                                                                                                                        <  2:645374/150‑1 (MQ=255)
            atatGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTcc                                                                                                                                        <  2:2262607/150‑1 (MQ=255)
               tGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGc                                                                                                                                     <  2:2123780/150‑1 (MQ=255)
                 ttGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTc                                                                                                                                   >  2:3213055/1‑150 (MQ=255)
                  tGAAAAGCCCGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTcc                                                                                                                                  >  2:4025430/1‑150 (MQ=255)
                  tGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTcc                                                                                                                                  <  2:4082528/150‑1 (MQ=255)
                   gAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCg                                                                                                                                 >  1:3650771/1‑150 (MQ=255)
                   gAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCg                                                                                                                                 <  1:1612809/150‑1 (MQ=255)
                      aaGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCca                                                                                                                              >  2:3525559/1‑150 (MQ=255)
                      aaGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCca                                                                                                                              <  1:3624849/150‑1 (MQ=255)
                      aaGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCca                                                                                                                              >  2:3861186/1‑150 (MQ=255)
                         ccAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACAt                                                                                                                           <  2:752456/150‑1 (MQ=255)
                           aGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCa                                                                                                                         >  2:631624/1‑150 (MQ=255)
                            gATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCaa                                                                                                                        <  2:1853169/150‑1 (MQ=255)
                             aTTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAg                                                                                                                       <  1:3502799/150‑1 (MQ=255)
                               tCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGa                                                                                                                     >  1:1718306/1‑150 (MQ=255)
                                cATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAt                                                                                                                    <  1:815181/150‑1 (MQ=255)
                                cATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGAt                                                                                                                    <  1:906475/150‑1 (MQ=255)
                                 aTAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATg                                                                                                                   >  2:3088879/1‑150 (MQ=255)
                                  tAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATgg                                                                                                                  <  2:793595/150‑1 (MQ=255)
                                   aaaGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGc                                                                                                                 <  1:2704185/150‑1 (MQ=255)
                                   aaaGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGc                                                                                                                 <  1:1757415/150‑1 (MQ=255)
                                    aaGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCg                                                                                                                >  2:4119864/1‑150 (MQ=255)
                                     aGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGt                                                                                                               <  2:1061713/150‑1 (MQ=255)
                                     aGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGt                                                                                                               >  1:2775306/1‑150 (MQ=255)
                                       cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                             >  2:2337867/1‑150 (MQ=255)
                                       cgcgTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                             >  1:3297080/1‑150 (MQ=255)
                                       cgcgTCATCTCCTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTgg                                                                                                             >  1:1333977/1‑150 (MQ=255)
                                             aTCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgtg                                                                                                       <  1:1000213/150‑1 (MQ=255)
                                             aTCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgtg                                                                                                       >  2:4139783/1‑150 (MQ=255)
                                             aTCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgtg                                                                                                       >  2:907609/1‑150 (MQ=255)
                                              tctcTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAgtgt                                                                                                      <  2:137239/150‑1 (MQ=255)
                                                 ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                                   <  1:582388/150‑1 (MQ=255)
                                                 ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                                   >  2:1169277/1‑150 (MQ=255)
                                                 ctTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGtgttgt                                                                                                   <  1:2199786/150‑1 (MQ=255)
                                                     ctctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAttt                                                                                               <  2:939846/150‑1 (MQ=255)
                                                     ctctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAttt                                                                                               <  2:2980139/150‑1 (MQ=255)
                                                     ctctGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTAttt                                                                                               <  1:3842301/150‑1 (MQ=255)
                                                        tGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGt                                                                                            <  1:1861705/150‑1 (MQ=255)
                                                         gACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTc                                                                                           >  2:2871966/1‑150 (MQ=255)
                                                             cccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGaa                                                                                       >  2:4164893/1‑150 (MQ=255)
                                                             cccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGaa                                                                                       >  2:2117302/1‑150 (MQ=255)
                                                              ccAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAat                                                                                      <  2:784433/150‑1 (MQ=255)
                                                               cAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAata                                                                                     >  2:3388630/1‑150 (MQ=255)
                                                                 gcgcGGCACATAGTCAGCCCCACCCCACCCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATc                                                                                   <  2:1707628/150‑1 (MQ=255)
                                                                    cgGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTc                                                                                <  1:1972294/150‑1 (MQ=255)
                                                                     ggCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               <  1:2778948/150‑1 (MQ=255)
                                                                     ggCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTcc                                                                               >  1:2525338/1‑150 (MQ=255)
                                                                      gCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCa                                                                              <  2:191925/150‑1 (MQ=255)
                                                                       cacaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAg                                                                             >  2:2949437/1‑150 (MQ=255)
                                                                       cacaTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAg                                                                             <  1:189327/150‑1 (MQ=255)
                                                                          aTAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCa                                                                          <  1:3784815/150‑1 (MQ=255)
                                                                              tCAGCCCCACCCCACCCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGAGGAGAACTCAACACCGCATTCAATAAACCGTCCAGCTCCGCCACATCAATGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTGCAGCCATTcc                                                                      <  2:390099/150‑1 (MQ=255)
                                                                               cAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCa                                                                     >  1:1471346/1‑150 (MQ=255)
                                                                                aGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCaa                                                                    >  1:2784025/1‑150 (MQ=255)
                                                                                aGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCaa                                                                    >  2:852580/1‑150 (MQ=255)
                                                                                   cccaccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTc                                                                 >  2:3495843/1‑150 (MQ=255)
                                                                                     caccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAATGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCAc                                                               >  1:469525/1‑150 (MQ=255)
                                                                                      accccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACt                                                              >  1:354102/1‑150 (MQ=255)
                                                                                      accccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACt                                                              >  2:3060527/1‑150 (MQ=255)
                                                                                       ccccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACtt                                                             <  2:2733695/150‑1 (MQ=255)
                                                                                         ccacACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTc                                                           >  1:1908193/1‑150 (MQ=255)
                                                                                          cacaCGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCg                                                          <  1:421824/150‑1 (MQ=255)
                                                                                               ggCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTaa                                                     >  1:2579595/1‑150 (MQ=255)
                                                                                                gCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAAc                                                    <  1:2904412/150‑1 (MQ=255)
                                                                                                   aCCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGt                                                 <  1:1272911/150‑1 (MQ=255)
                                                                                                    ccAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTc                                                <  1:2460936/150‑1 (MQ=255)
                                                                                                      aGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTccc                                              >  2:701882/1‑150 (MQ=255)
                                                                                                         cACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGtt                                           <  2:2652849/150‑1 (MQ=255)
                                                                                                            tGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTc                                        <  2:1139462/150‑1 (MQ=255)
                                                                                                              ttCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCAt                                      <  1:1655574/150‑1 (MQ=255)
                                                                                                                    ttCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtt                                >  1:73181/1‑150 (MQ=255)
                                                                                                                     tCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCttt                               >  1:2574327/1‑150 (MQ=255)
                                                                                                                      cAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtttt                              <  1:1410941/150‑1 (MQ=255)
                                                                                                                      cAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCtttt                              <  2:2604245/150‑1 (MQ=255)
                                                                                                                         tctcTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCt                           >  2:1946269/1‑150 (MQ=255)
                                                                                                                          ctctTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCtt                          <  1:1651600/150‑1 (MQ=255)
                                                                                                                             ttGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTtgtg                       <  2:3650771/150‑1 (MQ=255)
                                                                                                                              tGCGGAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTgg                      >  1:1095314/1‑150 (MQ=255)
                                                                                                                                 ggAGAACTCAACACCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCTTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATAAGCTTTTCCTTGGGGAtc                   >  1:1351878/1‑150 (MQ=255)
                                                                                                                                   agaACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtctc                 <  1:3773389/150‑1 (MQ=255)
                                                                                                                                   agaACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGAtctc                 >  1:3167006/1‑150 (MQ=255)
                                                                                                                                      aCTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAAc              >  1:322888/1‑150 (MQ=255)
                                                                                                                                       cTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACt             >  2:3187311/1‑150 (MQ=255)
                                                                                                                                         cAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCg           >  2:3284832/1‑150 (MQ=255)
                                                                                                                                           acacCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGcc         <  1:1524845/150‑1 (MQ=255)
                                                                                                                                           acacCGCAGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGcc         <  2:1883338/150‑1 (MQ=255)
                                                                                                                                              ccgccgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGc      >  1:2084700/1‑150 (MQ=255)
                                                                                                                                               cgccgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCa     >  2:952101/1‑150 (MQ=255)
                                                                                                                                                  cgTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCCCTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCATCTCGCCAGCACCt  >  2:3685567/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GCGGTATCGGCCATATGTTGAAAAGCCAGATTCATAAAGCGCGTCATCTCTTTCTCTGACGCCCAGCGCGGCACATAGTCAGCCCCACCCCACACGGCAACCAGCCACTGTTCCGGTTCAATCTCTTGCGGAGAACTCAACACCGCCGTCAATAAACCGTCCAGCTCCGCCACATCAAGGATGGCGTGGTCAGTGTTGTATTTGGTCAGAATATCGTCCAGCCATTCCAACTCACTTTCGTTTAACGGTCCCGTTTTCATACGCTTTTCCTTGTGGATCTCAACTCGCCAGCACCT  >  CP009273/1984841‑1985136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑