Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,043,389 G→T 7.5% L1657F (TTG→TTT)  yeeJ → putative adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,043,3890GT 7.5% 1110.2 / 40.4 288L1657F (TTG→TTT) yeeJputative adhesin
Reads supporting (aligned to +/- strand):  ref base G (133/133);  new base T (11/11);  total (144/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 288 total aligned reads displayed.

CAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGA  >  CP009273/2043241‑2043536
                                                                                                                                                    |                                                                                                                                                   
cAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGa                                                                                                                                                    >  2:3742336/1‑150 (MQ=255)
 aaGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGAt                                                                                                                                                   >  2:3028184/1‑150 (MQ=255)
  aGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGAtt                                                                                                                                                  >  1:3148377/1‑150 (MQ=255)
  aGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGAtt                                                                                                                                                  <  1:2425261/150‑1 (MQ=255)
    cTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGc                                                                                                                                                >  1:3570963/1‑150 (MQ=255)
        aCTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGAt                                                                                                                                            <  1:513565/150‑1 (MQ=255)
            tcGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTg                                                                                                                                        <  1:3593134/150‑1 (MQ=255)
             cGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTgg                                                                                                                                       >  1:1703714/1‑150 (MQ=255)
             cGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTgg                                                                                                                                       >  1:3157514/1‑150 (MQ=255)
              ggACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGt                                                                                                                                      <  1:3848176/150‑1 (MQ=255)
              ggACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGt                                                                                                                                      <  2:1147598/150‑1 (MQ=255)
               gACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTa                                                                                                                                     >  2:3506341/1‑150 (MQ=255)
                aCAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTAc                                                                                                                                    <  1:1915868/150‑1 (MQ=255)
                  aaGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCg                                                                                                                                  <  1:328357/150‑1 (MQ=255)
                    ggCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCa                                                                                                                                >  2:2418913/1‑150 (MQ=255)
                    ggCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCa                                                                                                                                >  2:3531508/1‑150 (MQ=255)
                         ttCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACt                                                                                                                           <  1:768519/150‑1 (MQ=255)
                            cGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGc                                                                                                                        <  1:3074878/150‑1 (MQ=255)
                                aTTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGcctcc                                                                                                                    >  2:3615713/1‑150 (MQ=255)
                                aTTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCCACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGcctcc                                                                                                                    <  2:2633283/150‑1 (MQ=255)
                                  tACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCtt                                                                                                                  <  1:3418720/150‑1 (MQ=255)
                                    cTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTaa                                                                                                                >  1:1442625/1‑150 (MQ=255)
                                     tGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAAc                                                                                                               <  2:2258820/150‑1 (MQ=255)
                                       atatCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACct                                                                                                             >  2:2004657/1‑150 (MQ=255)
                                        tatCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACctc                                                                                                            <  2:4033148/150‑1 (MQ=255)
                                          tCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTg                                                                                                          >  1:4069814/1‑150 (MQ=255)
                                           cAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGt                                                                                                         >  2:1238070/1‑150 (MQ=255)
                                           cAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGt                                                                                                         >  2:2690013/1‑150 (MQ=255)
                                           cAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGt                                                                                                         <  2:1505302/150‑1 (MQ=255)
                                           cAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGt                                                                                                         >  1:2132705/1‑150 (MQ=255)
                                             aCGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGtat                                                                                                       <  2:218464/150‑1 (MQ=255)
                                              cGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGtata                                                                                                      >  2:1768865/1‑150 (MQ=255)
                                              cGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGtata                                                                                                      >  2:1404919/1‑150 (MQ=255)
                                               ggCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATAc                                                                                                     >  2:663980/1‑150 (MQ=255)
                                                  aaGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCa                                                                                                  <  2:206746/150‑1 (MQ=255)
                                                    ggCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCAtt                                                                                                <  2:3272138/150‑1 (MQ=255)
                                                      cTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATtcg                                                                                              >  2:338938/1‑150 (MQ=255)
                                                      cTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATtcg                                                                                              >  2:184164/1‑150 (MQ=255)
                                                       tGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATtcgt                                                                                             >  2:4111329/1‑150 (MQ=255)
                                                         aaGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATtcgtcg                                                                                           >  2:390606/1‑150 (MQ=255)
                                                           gTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCgtcgtc                                                                                         <  2:230295/150‑1 (MQ=255)
                                                              acacTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGc                                                                                      >  1:2852459/1‑150 (MQ=255)
                                                                 cTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACg                                                                                   <  1:1961525/150‑1 (MQ=255)
                                                                 cTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACg                                                                                   <  2:238236/150‑1 (MQ=255)
                                                                   gAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGAc                                                                                 <  1:1013565/150‑1 (MQ=255)
                                                                    aGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGAcc                                                                                <  2:1544815/150‑1 (MQ=255)
                                                                       ggTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAg                                                                             <  1:2280832/150‑1 (MQ=255)
                                                                       ggTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAg                                                                             >  1:2315685/1‑150 (MQ=255)
                                                                       ggTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAg                                                                             >  1:2267746/1‑150 (MQ=255)
                                                                          aCAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACTACCGAGGgc                                                                          >  1:1435933/1‑150 (MQ=255)
                                                                          aCAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGgc                                                                          <  1:1510111/150‑1 (MQ=255)
                                                                           cAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGgcg                                                                         <  1:1129173/150‑1 (MQ=255)
                                                                             aaaTCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCa                                                                       >  2:540654/1‑150 (MQ=255)
                                                                             aaaTCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCa                                                                       >  1:1846255/1‑150 (MQ=255)
                                                                              aaTCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACATCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCaa                                                                      >  2:1170846/1‑150 (MQ=255)
                                                                               aTCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAAc                                                                     <  2:306479/150‑1 (MQ=255)
                                                                               aTCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAAc                                                                     <  2:3866796/150‑1 (MQ=255)
                                                                                  cGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCAt                                                                  <  2:3347145/150‑1 (MQ=255)
                                                                                     cACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGAc                                                               >  2:3536860/1‑150 (MQ=255)
                                                                                     cACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGAc                                                               >  2:1439684/1‑150 (MQ=255)
                                                                                      aCCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACg                                                              >  1:1341218/1‑150 (MQ=255)
                                                                                      aCCGACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACg                                                              <  2:1049026/150‑1 (MQ=255)
                                                                                                tGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCa                                                    >  1:2072568/1‑150 (MQ=255)
                                                                                                 gACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCAc                                                   >  1:3800345/1‑150 (MQ=255)
                                                                                                  aCAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACt                                                  <  1:3160328/150‑1 (MQ=255)
                                                                                                  aCAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACt                                                  <  1:444944/150‑1 (MQ=255)
                                                                                                   cAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTg                                                 <  1:2681936/150‑1 (MQ=255)
                                                                                                    aGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGa                                                <  2:429881/150‑1 (MQ=255)
                                                                                                        aGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCt                                            >  1:562591/1‑150 (MQ=255)
                                                                                                         gCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTa                                           <  1:1717798/150‑1 (MQ=255)
                                                                                                           gTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAAc                                         <  1:3485907/150‑1 (MQ=255)
                                                                                                            tGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACg                                        <  2:3838433/150‑1 (MQ=255)
                                                                                                                   ttATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAccc                                 <  2:1686327/150‑1 (MQ=255)
                                                                                                                   ttATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAccc                                 >  1:2182070/1‑150 (MQ=255)
                                                                                                                   ttATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAAccc                                 >  1:649091/1‑150 (MQ=255)
                                                                                                                        gtgtCAGTGATACGAAACAGGTGACTTTTATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAg                            <  1:802367/150‑1 (MQ=255)
                                                                                                                        gtgtCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAg                            <  2:3679829/150‑1 (MQ=255)
                                                                                                                           tCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAg                         >  2:2801550/1‑150 (MQ=255)
                                                                                                                               tGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCAt                     >  1:947209/1‑150 (MQ=255)
                                                                                                                                gATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATa                    <  2:2422004/150‑1 (MQ=255)
                                                                                                                                  tACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaa                  <  2:310009/150‑1 (MQ=255)
                                                                                                                                   aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                 <  2:231981/150‑1 (MQ=255)
                                                                                                                                   aCGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATaaaa                 >  2:3992984/1‑150 (MQ=255)
                                                                                                                                    cGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAg                >  2:509922/1‑150 (MQ=255)
                                                                                                                                    cGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAg                >  2:605108/1‑150 (MQ=255)
                                                                                                                                     gAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGt               <  1:3927151/150‑1 (MQ=255)
                                                                                                                                        aCAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTaa            >  2:1934027/1‑150 (MQ=255)
                                                                                                                                          aGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAtt          >  1:2656953/1‑150 (MQ=255)
                                                                                                                                          aGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAtt          >  2:2740797/1‑150 (MQ=255)
                                                                                                                                          aGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAtt          >  2:1609937/1‑150 (MQ=255)
                                                                                                                                          aGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAtt          <  1:1203084/150‑1 (MQ=255)
                                                                                                                                           ggTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAttt         <  1:1778853/150‑1 (MQ=255)
                                                                                                                                           ggTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAAttt         >  2:26230/1‑150 (MQ=255)
                                                                                                                                            gTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATATc        >  1:3233558/1‑150 (MQ=255)
                                                                                                                                              gACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCcg      >  2:3247376/1‑150 (MQ=255)
                                                                                                                                                cTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCcgcg    <  2:1577425/150‑1 (MQ=255)
                                                                                                                                                 tttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCgg   >  2:2492289/1‑150 (MQ=255)
                                                                                                                                                  ttGTTTGCCGATGCTGTTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGTGTGCGCACCCTTGACGGCAAGCGTCAGTGACGCTAACGTCAACCCGGTAGAAGGCATAAAAGTTAATTTGcgcgta  <  2:834596/150‑3 (MQ=255)
                                                                                                                                                  ttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGa  <  1:789739/150‑1 (MQ=255)
                                                                                                                                                  ttGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGa  >  2:2655655/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
CAAGCTTTACTCTCGGACAAGGCGGTTCCGCCATTACTGATATCAACGGCAAGGCTGAAGTTACACTGAGCGGTACAAAATCCGGCACCTACCCCGTGACAGTTAGCGTGAACAATTATGGTGTCAGTGATACGAAACAGGTGACTTTGATTGCCGATGCTGGTACCGCAAAACTAGCCTCCTTAACCTCTGTATACTCATTCGTCGTCAGCACGACCGAGGGCGCAACCATGACGGCAAGCGTCACTGACGCTAACGGCAACCCGGTAGAAGGCATAAAAGTTAATTTCCGCGGA  >  CP009273/2043241‑2043536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑