BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,050,363	G→A	9.6%	V9I (GTT→ATT)	yeeN →	UPF0082 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,050,363	0	G	A	9.6%	728.9 / 47.6	219	V9I (GTT→ATT)	yeeN	UPF0082 family protein
Reads supporting (aligned to +/- strand): ref base G (86/112); new base A (12/9); total (98/121)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

Only 100 of 233 total aligned reads displayed.

TATGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAGGCACAA  >  CP009273/2050215‑2050512

 aTGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATAttgtt                                                                                                                                                     >  1:1354873/1‑150 (MQ=255)

 aTGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATAttgtt                                                                                                                                                     <  2:3697537/150‑1 (MQ=255)

 aTGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATAttgtt                                                                                                                                                     >  1:246863/1‑150 (MQ=255)

  tGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATAttgttg                                                                                                                                                    >  1:3408806/1‑150 (MQ=255)

    gCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATAttgttgtt                                                                                                                                                  >  2:1873090/1‑148 (MQ=255)

    gCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCt                                                                                                                                                  <  2:1102868/150‑1 (MQ=255)

       ttAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaa                                                                                                                                               <  1:1814286/150‑1 (MQ=255)

         aGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaa                                                                                                                                             >  2:595492/1‑150 (MQ=255)

         aGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaa                                                                                                                                             >  2:1234772/1‑150 (MQ=255)

          gAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaaa                                                                                                                                            <  2:1240023/150‑1 (MQ=255)

           aaTATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTaaaaaaa                                                                                                                                           <  2:1325640/150‑1 (MQ=255)

             tatTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACg                                                                                                                                         <  2:1026001/150‑1 (MQ=255)

             tatTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACg                                                                                                                                         >  1:2720571/1‑150 (MQ=255)

                ttttATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCt                                                                                                                                      <  1:2252214/150‑1 (MQ=255)

                   tATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTaaa                                                                                                                                   >  2:1947327/1‑150 (MQ=255)

                         tAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGt                                                                                                                             <  1:2072796/150‑1 (MQ=255)

                          aaTAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTg                                                                                                                            >  1:3098800/1‑150 (MQ=255)

                          aaTAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTg                                                                                                                            >  1:3098625/1‑150 (MQ=255)

                          aaTAGACGAGATTTTTCCTGTTATGATATAATATGCCGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTg                                                                                                                            >  1:198111/1‑150 (MQ=255)

                              gACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAAc                                                                                                                        >  1:3504307/1‑150 (MQ=255)

                               aCGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACg                                                                                                                       <  2:3714489/150‑1 (MQ=255)

                               aCGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACg                                                                                                                       <  1:653978/150‑1 (MQ=255)

                                   gaTTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTa                                                                                                                   >  2:425584/1‑150 (MQ=255)

                                    aTTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTaa                                                                                                                  >  1:2400189/1‑150 (MQ=255)

                                    aTTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTaa                                                                                                                  <  2:45283/150‑1 (MQ=255)

                                         tCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAAttt                                                                                                             >  1:853737/1‑150 (MQ=255)

                                           cTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTAt                                                                                                           >  2:3293344/1‑150 (MQ=255)

                                           cTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTAt                                                                                                           <  2:512009/150‑1 (MQ=255)

                                           cTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTAt                                                                                                           <  2:625686/150‑1 (MQ=255)

                                              ttATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCa                                                                                                        <  2:1632215/150‑1 (MQ=255)

                                               tATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaa                                                                                                       >  1:3071450/1‑150 (MQ=255)

                                                 tGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaaaa                                                                                                     <  2:807341/150‑1 (MQ=255)

                                                 tGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCaaaa                                                                                                     <  2:2849838/150‑1 (MQ=255)

                                                    tataATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTc                                                                                                  >  1:3875345/1‑150 (MQ=255)

                                                    tataATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTc                                                                                                  >  2:3209526/1‑150 (MQ=255)

                                                         tatGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGgtgt                                                                                             <  1:108006/150‑1 (MQ=255)

                                                             cTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGaa                                                                                         >  2:1982070/1‑150 (MQ=255)

                                                               gAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAAt                                                                                       >  1:2505271/1‑150 (MQ=255)

                                                                 aTTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCt                                                                                     <  2:2277758/150‑1 (MQ=255)

                                                                     acacATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgc                                                                                 >  2:1356638/1‑150 (MQ=255)

                                                                      cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTCTGCAAAATTCGGTGTAGAAATCTAtgct                                                                                >  1:1480907/1‑150 (MQ=255)

                                                                      cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgct                                                                                >  1:1137524/1‑150 (MQ=255)

                                                                      cacaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGTCGGTGCAACGTCTAATATTTATGCAAAATTCGGTGTAGAAATATAtgct                                                                                <  2:1184127/150‑1 (MQ=255)

                                                                       acaTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctg                                                                               >  2:672513/1‑150 (MQ=255)

                                                                         aTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgct                                                                             <  1:49520/150‑1 (MQ=255)

                                                                         aTGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTAtgctgct                                                                             <  2:1381873/150‑1 (MQ=255)

                                                                                 gATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACa                                                                     >  1:2297537/1‑150 (MQ=255)

                                                                                  aTTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAATCGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACaa                                                                    >  2:804184/1‑150 (MQ=255)

                                                                                  aTTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACaa                                                                    >  2:1602957/1‑150 (MQ=255)

                                                                                   tttACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAg                                                                   >  1:2532405/1‑150 (MQ=255)

                                                                                      aCCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTg                                                                <  2:2035395/150‑1 (MQ=255)

                                                                                      aCCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTg                                                                <  2:1882649/150‑1 (MQ=255)

                                                                                       ccAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGa                                                               >  2:537453/1‑150 (MQ=255)

                                                                                       ccAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGa                                                               <  1:2436519/150‑1 (MQ=255)

                                                                                        cAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGaa                                                              >  2:313649/1‑150 (MQ=255)

                                                                                         aGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAc                                                             <  1:217881/150‑1 (MQ=255)

                                                                                          gTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                                            <  1:1078789/150‑1 (MQ=255)

                                                                                          gTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAAcc                                                            <  2:930730/150‑1 (MQ=255)

                                                                                            aaTGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCg                                                          <  2:2724771/150‑1 (MQ=255)

                                                                                               gTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATc                                                       <  2:566912/150‑1 (MQ=255)

                                                                                               gTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATc                                                       >  1:3279210/1‑150 (MQ=255)

                                                                                                taaataaaTTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATcc                                                      <  1:1901916/150‑1 (MQ=255)

                                                                                                     aaatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGTTGAACCCGATCCAGAAt                                                 >  2:621504/1‑150 (MQ=255)

                                                                                                     aaatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAAt                                                 <  1:1336082/150‑1 (MQ=255)

                                                                                                      aatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACTAGGTGAACCCGATCCAGAAtt                                                >  2:2158673/1‑150 (MQ=255)

                                                                                                      aatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAAtt                                                >  2:2389166/1‑150 (MQ=255)

                                                                                                      aatTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATATATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAAtt                                                >  1:3985417/1‑150 (MQ=255)

                                                                                                       atTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTa                                               >  2:4097333/1‑150 (MQ=255)

                                                                                                         tttCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTaaa                                             <  1:3455811/150‑1 (MQ=255)

                                                                                                          ttCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAac                                            <  1:384615/150‑1 (MQ=255)

                                                                                                             gaggagATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAAcaca                                         >  2:4169442/1‑150 (MQ=255)

                                                                                                             gaggagATCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAAcaca                                         >  1:575423/1‑150 (MQ=255)

                                                                                                               ggagATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATc                                       <  1:2026017/150‑1 (MQ=255)

                                                                                                                gagaTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCt                                      <  2:934159/150‑1 (MQ=255)

                                                                                                                gagaTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCt                                      <  1:3813546/150‑1 (MQ=255)

                                                                                                                 agaTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCtt                                     >  2:3573736/1‑150 (MQ=255)

                                                                                                                 agaTCATTCCAGTGGGACGTAAATGGGCCAATATTATTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCtt                                     >  1:1801085/1‑150 (MQ=255)

                                                                                                                   aTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTa                                   >  1:416467/1‑150 (MQ=255)

                                                                                                                   aTCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTa                                   >  1:323942/1‑150 (MQ=255)

                                                                                                                    tCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTaa                                  >  2:2775336/1‑150 (MQ=255)

                                                                                                                     cATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTaaa                                 <  1:960672/150‑1 (MQ=255)

                                                                                                                      aTTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTaaaa                                <  2:4003359/150‑1 (MQ=255)

                                                                                                                       ttCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAAt                               <  1:1554271/150‑1 (MQ=255)

                                                                                                                           aGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGt                           >  2:155356/1‑150 (MQ=255)

                                                                                                                             tGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTa                         <  1:649975/150‑1 (MQ=255)

                                                                                                                                gACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAACACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTg                      <  1:3121567/150‑1 (MQ=255)

                                                                                                                                    tAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACg                  <  1:1611046/150‑1 (MQ=255)

                                                                                                                                    tAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACg                  >  2:3491371/1‑150 (MQ=255)

                                                                                                                                     aaaTGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGt                 >  2:1198976/1‑150 (MQ=255)

                                                                                                                                      aaTGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTg                <  2:246592/150‑1 (MQ=255)

                                                                                                                                         gggCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCaa             >  2:3899367/1‑150 (MQ=255)

                                                                                                                                          ggCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCaaa            <  1:747963/150‑1 (MQ=255)

                                                                                                                                           gCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAg           <  2:906515/150‑1 (MQ=255)

                                                                                                                                             cAATCTTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCa         <  2:2017812/150‑1 (MQ=255)

                                                                                                                                             cAATATTGTTGCTAAAAAAACGGCTAACGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCa         <  2:374537/150‑1 (MQ=255)

                                                                                                                                             cAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAATGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCa         >  2:2197733/1‑150 (MQ=255)

                                                                                                                                              aaTATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAg        <  1:1105739/150‑1 (MQ=255)

                                                                                                                                                 attattGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAGGca     >  1:1227784/1‑150 (MQ=255)

                                                                                                                                                 atTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCTATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAGGca     >  2:3536969/1‑150 (MQ=255)

                                                                                                                                                 atTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAGGca     >  2:1844787/1‑150 (MQ=255)

TATGGCTTTAGAATATTTTTATTTCTAATAGACGAGATTTTTCCTGTTATGATATAATATGCTGAATTAACACATGTTAACGATTTACCAGTAATGTAAATAAATTTTCGAGGAGATCATTCCAGTGGGACGTAAATGGGCCAATATTGTTGCTAAAAAAACGGCTAAAGACGGTGCAACGTCTAAAATTTATGCAAAATTCGGTGTAGAAATCTATGCTGCTGCTAAACAAGGTGAACCCGATCCAGAATTAAACACATCTTTAAAATTCGTTATTGAACGTGCAAAGCAGGCACAA  >  CP009273/2050215‑2050512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`