Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,115,361 C→A 42.7% R367L (CGC→CTC)  cpsG ← phosphomannomutase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,115,3610CA42.7% 262.8 / inf 262R367L (CGC→CTC) cpsGphosphomannomutase
Reads supporting (aligned to +/- strand):  ref base C (64/86);  new base A (46/66);  total (110/152)
Fisher's exact test for biased strand distribution p-value = 8.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 267 total aligned reads displayed.

TCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115214‑2115510
                                                                                                                                                   |                                                                                                                                                     
tCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGt                                                                                                                                                     <  2:3712361/150‑1 (MQ=255)
  ggCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTAc                                                                                                                                                   >  2:2250944/1‑150 (MQ=255)
  ggCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTAc                                                                                                                                                   >  1:1488539/1‑150 (MQ=255)
    cAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCa                                                                                                                                                 <  2:1470739/150‑1 (MQ=255)
    cAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCa                                                                                                                                                 <  1:74939/150‑1 (MQ=255)
     aaaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAg                                                                                                                                                >  1:720641/1‑150 (MQ=255)
      aaGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGt                                                                                                                                               <  1:476769/150‑1 (MQ=255)
        ggTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTc                                                                                                                                             >  2:1740760/1‑150 (MQ=255)
         gTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCg                                                                                                                                            <  2:3619019/150‑1 (MQ=255)
         gTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCg                                                                                                                                            <  2:2034171/150‑1 (MQ=255)
            aTGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGccc                                                                                                                                         <  1:2447610/150‑1 (MQ=255)
               cTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGc                                                                                                                                      <  1:1855184/150‑1 (MQ=255)
               cTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGc                                                                                                                                      >  2:1521023/1‑150 (MQ=255)
                tGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCg                                                                                                                                     <  2:2816802/150‑1 (MQ=255)
                 gATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGt                                                                                                                                    <  1:3003503/150‑1 (MQ=255)
                    gCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGtttt                                                                                                                                 <  1:3031898/150‑1 (MQ=255)
                     ccATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTa                                                                                                                                >  2:674871/1‑150 (MQ=255)
                     ccATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTa                                                                                                                                >  1:2247578/1‑150 (MQ=255)
                        tCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCt                                                                                                                             <  2:2876677/150‑1 (MQ=255)
                         cGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCCGTTCGCCCAGCGTGTTATCtt                                                                                                                            >  2:3240981/1‑150 (MQ=255)
                         cGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCtt                                                                                                                            <  1:1066314/150‑1 (MQ=255)
                                aTCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGca                                                                                                                     >  2:1323624/1‑150 (MQ=255)
                                  ccACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGcaca                                                                                                                   <  1:3995824/150‑1 (MQ=255)
                                    aCCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACAcc                                                                                                                 <  1:2154265/150‑1 (MQ=255)
                                    aCCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACAcc                                                                                                                 >  1:2827439/1‑150 (MQ=255)
                                       gCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGt                                                                                                              <  2:3843776/150‑1 (MQ=255)
                                          aGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCg                                                                                                           <  1:837711/150‑1 (MQ=255)
                                          aGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCg                                                                                                           >  2:2410336/1‑150 (MQ=255)
                                          aGCGCCTCACGGCTAAAATGATGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCg                                                                                                           <  1:837776/150‑1 (MQ=255)
                                           gcgcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCgg                                                                                                          >  1:2788962/1‑150 (MQ=255)
                                             gcCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCg                                                                                                        <  2:897315/150‑1 (MQ=255)
                                                 cACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACca                                                                                                    <  1:2686651/150‑1 (MQ=255)
                                                 cACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACca                                                                                                    >  1:2745583/1‑150 (MQ=255)
                                                  aCGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcag                                                                                                   <  1:3173460/150‑1 (MQ=255)
                                                     gCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagcag                                                                                                >  2:1423885/1‑150 (MQ=255)
                                                     gCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACcagcag                                                                                                <  2:1207167/150‑1 (MQ=255)
                                                         aaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCAc                                                                                            <  2:1093075/150‑1 (MQ=255)
                                                         aaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCAc                                                                                            <  2:535531/150‑1 (MQ=255)
                                                         aaaTGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCAc                                                                                            >  2:2533241/1‑150 (MQ=255)
                                                             gCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGa                                                                                        <  2:1981737/150‑1 (MQ=255)
                                                              cTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGAt                                                                                       <  1:3151496/150‑1 (MQ=255)
                                                                 ttCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCAt                                                                                    <  2:3446215/150‑1 (MQ=255)
                                                                  tCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATg                                                                                   <  1:1790573/150‑1 (MQ=255)
                                                                    cACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGcc                                                                                 <  1:971209/150‑1 (MQ=255)
                                                                         ggTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGt                                                                            >  1:3387756/1‑150 (MQ=255)
                                                                         ggTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGt                                                                            <  1:1177795/150‑1 (MQ=255)
                                                                          gTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTc                                                                           <  1:2961025/150‑1 (MQ=255)
                                                                           ttAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCg                                                                          <  1:2992672/150‑1 (MQ=255)
                                                                            tAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGc                                                                         >  1:2187670/1‑150 (MQ=255)
                                                                             aaTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCa                                                                        >  2:267184/1‑150 (MQ=255)
                                                                             aaTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCa                                                                        <  2:257636/150‑1 (MQ=255)
                                                                             aaTCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCa                                                                        >  2:3531589/1‑150 (MQ=255)
                                                                                cGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTa                                                                     <  1:163549/150‑1 (MQ=255)
                                                                                 gCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTaa                                                                    >  2:2494107/1‑150 (MQ=255)
                                                                                  ccTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAg                                                                   >  2:2807761/1‑150 (MQ=255)
                                                                                  ccTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAg                                                                   >  1:3670887/1‑150 (MQ=255)
                                                                                         gggTTGCGCCAGTTTTCTGTTGTTCTCTCCGCTTGCCGGATACGCCGCCATCCGGTCGAGTACCATTTCGCCCAGCGTTTTATCTTTCATGCACACCAGTTCGGCGACCAGCAGCCTCGGGATCTTGCCGCTGTCGCAGTAAGCGAAATc                                                            <  1:3655770/150‑1 (MQ=255)
                                                                                          ggTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCa                                                           <  1:3408309/150‑1 (MQ=255)
                                                                                           gTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCAc                                                          >  1:905468/1‑150 (MQ=255)
                                                                                           gTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCAc                                                          >  1:2557324/1‑150 (MQ=255)
                                                                                            ttGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACg                                                         <  2:396029/150‑1 (MQ=255)
                                                                                            ttGCGCCAGTTTGCTGTTGATCTCACAGCTTGCCGGAAACGCCGCCATCCGGTCGAGTTCCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGGAGTAAGCGAAATCAct                                                         <  2:1595595/150‑2 (MQ=255)
                                                                                               cgcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGaa                                                      <  2:1363808/150‑1 (MQ=255)
                                                                                                gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAg                                                     <  1:1386153/150‑1 (MQ=255)
                                                                                                gcCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAg                                                     >  2:3776450/1‑150 (MQ=255)
                                                                                                 ccAGTTTGCTGTTGATCTCCCCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGt                                                    <  1:469154/150‑1 (MQ=255)
                                                                                                  cAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTGTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTa                                                   >  1:4071148/1‑150 (MQ=255)
                                                                                                   aGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTaa                                                  >  2:3408166/1‑150 (MQ=255)
                                                                                                    gTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAt                                                 >  1:2027269/1‑150 (MQ=255)
                                                                                                     tttGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtg                                                <  1:3970962/150‑1 (MQ=255)
                                                                                                      ttGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtgg                                               >  2:696755/1‑150 (MQ=255)
                                                                                                         cTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAtggtgg                                            <  2:3036468/150‑1 (MQ=255)
                                                                                                              gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                       >  1:1072198/1‑150 (MQ=255)
                                                                                                              gATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCt                                       >  2:1785700/1‑150 (MQ=255)
                                                                                                                tctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCa                                     <  1:3605063/150‑1 (MQ=255)
                                                                                                                tctcACCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCa                                     <  1:3363058/150‑1 (MQ=255)
                                                                                                                    aCCGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTc                                 >  2:2993127/1‑150 (MQ=255)
                                                                                                                     ccGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg                                <  2:2788433/150‑1 (MQ=255)
                                                                                                                     ccGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg                                <  1:4169148/150‑1 (MQ=255)
                                                                                                                     ccGCTTGCCGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCg                                >  1:3870860/1‑150 (MQ=255)
                                                                                                                          tGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGccacc                           >  1:1244870/1‑150 (MQ=255)
                                                                                                                             cGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATa                        <  1:1902545/150‑1 (MQ=255)
                                                                                                                             cGGAAACGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATa                        >  2:3807309/1‑150 (MQ=255)
                                                                                                                              ggAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGGTATATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAAAGGGGGGCGCTCATTTCGCCACCATAg                       >  2:3139727/1‑150 (MQ=255)
                                                                                                                              ggAAACGCCGCCATCCGGTCGAGTACCAGTNCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAg                       >  1:3853626/1‑150 (MQ=255)
                                                                                                                               gAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGa                      >  1:603874/1‑150 (MQ=255)
                                                                                                                                aaaCGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGAt                     <  1:1510234/150‑1 (MQ=255)
                                                                                                                                 aaCGCCGCCATCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATg                    <  1:648660/150‑1 (MQ=255)
                                                                                                                                  aCGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATgg                   <  2:2052236/150‑1 (MQ=255)
                                                                                                                                   cgccgcCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGc                  <  1:443764/150‑1 (MQ=255)
                                                                                                                                      cgccATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTc               >  2:2094133/1‑150 (MQ=255)
                                                                                                                                         cATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTc            <  2:1016560/150‑1 (MQ=255)
                                                                                                                                         cATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTc            <  2:1016456/150‑1 (MQ=255)
                                                                                                                                          aTCCGGTCGAGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTcc           <  2:3096471/150‑1 (MQ=255)
                                                                                                                                            ccGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt         >  2:3684179/1‑150 (MQ=255)
                                                                                                                                            ccGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCtt         <  2:1723710/150‑1 (MQ=255)
                                                                                                                                             cGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTg        <  2:611342/150‑1 (MQ=255)
                                                                                                                                               gTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTgcg      >  1:1828750/1‑150 (MQ=255)
                                                                                                                                                 cgcgTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCa    >  2:4176216/1‑150 (MQ=255)
                                                                                                                                                   cgTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATa  <  1:1858172/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TCGGCAAAGGTCATGCTGATGCCATCGGTGCGATCCACCGCCAGCGCCTCACGGCTAAAATGCTGTTCCACGCGGTTAATCGCCTCAACGGGTTGCGCCAGTTTGCTGTTGATCTCACCGCTTGCCGGAAACGCCGCCATCCGGTCGCGTACCAGTTCGCCCAGCGTTTTATCTTTCAGGCACACCAGTTCGGCGACCAGCAGCCACGGGATCATGCCGCTGTCGCAGTAAGCGAAATCACGGAAGTAATGGTGGGCGCTCATTTCGCCACCATAGATGGCGTCTTCCTTGCGCATA  >  CP009273/2115214‑2115510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑