Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,121,234 C→A 36.1% E188* (GAA→TAA)  gmd ← GDP‑D‑mannose dehydratase, NAD(P)‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,121,2340CA36.1% 315.3 / 212.2 208E188* (GAA→TAA) gmdGDP‑D‑mannose dehydratase, NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (55/78);  new base A (39/36);  total (94/114)
Fisher's exact test for biased strand distribution p-value = 1.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

Only 100 of 215 total aligned reads displayed.

CTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAC  >  CP009273/2121085‑2121383
                                                                                                                                                     |                                                                                                                                                     
cTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTc                                                                                                                                                       <  2:1444744/150‑1 (MQ=255)
cTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTc                                                                                                                                                       <  2:2431764/150‑1 (MQ=255)
   cATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATg                                                                                                                                                    <  1:4087068/150‑1 (MQ=255)
     tttttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGt                                                                                                                                                  <  2:3772238/150‑1 (MQ=255)
     tttttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGt                                                                                                                                                  >  2:1367533/1‑150 (MQ=255)
      ttttACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGtt                                                                                                                                                 <  1:190556/150‑1 (MQ=255)
            gTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAagag                                                                                                                                           >  2:3632303/1‑150 (MQ=255)
             tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGACTCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAgaga                                                                                                                                          >  2:2052450/1‑150 (MQ=255)
             tAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAgaga                                                                                                                                          >  2:3956576/1‑150 (MQ=255)
                tCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAAtt                                                                                                                                       <  2:431267/150‑1 (MQ=255)
                 cTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTc                                                                                                                                      >  1:447336/1‑150 (MQ=255)
                 cTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTc                                                                                                                                      >  2:2260866/1‑150 (MQ=255)
                  tttGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTcc                                                                                                                                     >  1:513917/1‑150 (MQ=255)
                    tGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGt                                                                                                                                   >  1:3575348/1‑150 (MQ=255)
                      gCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTa                                                                                                                                 <  2:1657875/150‑1 (MQ=255)
                       cGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTAc                                                                                                                                >  1:3337213/1‑150 (MQ=255)
                       cGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTAc                                                                                                                                >  1:3620214/1‑150 (MQ=255)
                        gTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACa                                                                                                                               >  1:2417910/1‑150 (MQ=255)
                        gTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACa                                                                                                                               >  1:2202816/1‑150 (MQ=255)
                          ggCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAgg                                                                                                                             >  2:3181778/1‑150 (MQ=255)
                            ccccAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCg                                                                                                                           <  1:1937990/150‑1 (MQ=255)
                             cccAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGt                                                                                                                          <  1:3068375/150‑1 (MQ=255)
                               cAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTAc                                                                                                                        <  1:1356375/150‑1 (MQ=255)
                                aGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACa                                                                                                                       >  2:858092/1‑150 (MQ=255)
                                  tCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATg                                                                                                                     >  1:3224026/1‑150 (MQ=255)
                                     cGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCg                                                                                                                  <  1:1427549/150‑1 (MQ=255)
                                       cAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                <  2:1307353/150‑1 (MQ=255)
                                       cAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                <  2:1092196/150‑1 (MQ=255)
                                       cAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTa                                                                                                                <  2:1801271/150‑1 (MQ=255)
                                          ggAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGa                                                                                                             <  2:215932/150‑1 (MQ=255)
                                              tCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                         <  1:1417820/150‑1 (MQ=255)
                                              tCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTGGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCa                                                                                                         <  2:3742877/150‑1 (MQ=255)
                                               ccATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                                                                                        <  2:1660269/150‑1 (MQ=255)
                                               ccATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCAc                                                                                                        <  2:2903013/150‑1 (MQ=255)
                                                 atatTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACgg                                                                                                      <  1:2748234/150‑1 (MQ=255)
                                                  tatTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGt                                                                                                     >  1:3726505/1‑150 (MQ=255)
                                                  tatTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGt                                                                                                     >  2:1633773/1‑150 (MQ=255)
                                                  tatTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGt                                                                                                     <  1:1922154/150‑1 (MQ=255)
                                                   atTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTa                                                                                                    >  1:1618789/1‑150 (MQ=255)
                                                         gAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAAc                                                                                              <  2:2667575/150‑1 (MQ=255)
                                                            gTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGt                                                                                           <  2:2948490/150‑1 (MQ=255)
                                                               cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGAt                                                                                        >  1:1921851/1‑150 (MQ=255)
                                                               cAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGAt                                                                                        <  1:2864588/150‑1 (MQ=255)
                                                                aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATc                                                                                       <  2:402775/150‑1 (MQ=255)
                                                                aGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATc                                                                                       >  2:2022341/1‑150 (MQ=255)
                                                                   cACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                                                                                    <  1:588719/150‑1 (MQ=255)
                                                                   cACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAg                                                                                    <  2:500354/150‑1 (MQ=255)
                                                                    tcGACTCCAGCCCCGGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGt                                                                                   <  1:893886/149‑1 (MQ=255)
                                                                      gACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAg                                                                                 <  1:2918720/150‑1 (MQ=255)
                                                                       aCTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAgg                                                                                <  2:1390285/150‑1 (MQ=255)
                                                                        cTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGc                                                                               >  1:1270499/1‑150 (MQ=255)
                                                                           cAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTa                                                                            <  2:902560/150‑1 (MQ=255)
                                                                            aGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTAc                                                                           >  2:761084/1‑150 (MQ=255)
                                                                                   gggCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTgg                                                                    >  2:4081439/1‑150 (MQ=255)
                                                                                     gCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGCAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCg                                                                  <  2:2224309/150‑1 (MQ=255)
                                                                                     gCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCg                                                                  <  2:12416/150‑1 (MQ=255)
                                                                                      cGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGa                                                                 >  2:2117971/1‑150 (MQ=255)
                                                                                        aTGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGAcc                                                               >  1:2312416/1‑150 (MQ=255)
                                                                                            tGGCGATTGCGCGGGTGATTATGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGATAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCAt                                                           >  1:2055578/1‑150 (MQ=255)
                                                                                              gCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATAc                                                         <  1:2782745/150‑1 (MQ=255)
                                                                                                gATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                       >  2:252897/1‑150 (MQ=255)
                                                                                                gATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACgg                                                       <  2:3154654/150‑1 (MQ=255)
                                                                                                   tGCGCGGGTGATTTTGCGGGTAGCGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGgaga                                                    >  2:1206936/1‑150 (MQ=255)
                                                                                                   tGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGgaga                                                    >  2:235148/1‑150 (MQ=255)
                                                                                                     cgcgGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATc                                                  <  1:3710347/150‑1 (MQ=255)
                                                                                                        gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATcgcg                                               <  1:3005006/150‑1 (MQ=255)
                                                                                                        gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATcgcg                                               >  1:1050611/1‑150 (MQ=255)
                                                                                                        gggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATcgcg                                               <  1:1729855/150‑1 (MQ=255)
                                                                                                         ggTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCgg                                              >  1:3398018/1‑150 (MQ=255)
                                                                                                          gTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCggg                                             >  1:1137424/1‑150 (MQ=255)
                                                                                                           tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGt                                            <  1:400103/150‑1 (MQ=255)
                                                                                                           tGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGt                                            <  2:1880151/150‑1 (MQ=255)
                                                                                                               tttGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGaa                                        >  1:2556979/1‑150 (MQ=255)
                                                                                                                     ggTAACGAAGGTTTCGCCTCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTAACGGTAGTTAACGGTTATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGt                                  >  2:1123466/1‑150 (MQ=255)
                                                                                                                     ggTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGt                                  <  1:3264538/150‑1 (MQ=255)
                                                                                                                      gTAACGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTg                                 >  1:2057888/1‑150 (MQ=255)
                                                                                                                        aaCGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGt                               >  2:3233277/1‑150 (MQ=255)
                                                                                                                        aaCGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGt                               >  2:2994092/1‑150 (MQ=255)
                                                                                                                         aCGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtc                              <  2:3972090/150‑1 (MQ=255)
                                                                                                                          cGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtct                             <  2:192263/150‑1 (MQ=255)
                                                                                                                          cGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAAGTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtct                             <  2:3889009/150‑1 (MQ=255)
                                                                                                                          cGAAGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGtct                             <  2:3378600/150‑1 (MQ=255)
                                                                                                                            aaGGTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTctct                           <  1:3181778/150‑1 (MQ=255)
                                                                                                                             aGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCtt                          >  1:86588/1‑150 (MQ=255)
                                                                                                                               gTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTc                        >  2:3427953/1‑150 (MQ=255)
                                                                                                                               gTTTCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTc                        <  2:2473224/150‑1 (MQ=255)
                                                                                                                                  tCGCCGCGGCGCGGGGATTCATGGTTGAAGAGACTTCCGTTACTGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGc                     <  1:2352316/150‑1 (MQ=255)
                                                                                                                                  tCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGc                     <  2:4001999/150‑1 (MQ=255)
                                                                                                                                  tCGCCGCGGCGCGGGGATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGc                     >  1:2697622/1‑150 (MQ=255)
                                                                                                                                       gcggcgcggGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAAt                <  1:3545942/150‑1 (MQ=255)
                                                                                                                                            gcggGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt           <  1:546988/150‑1 (MQ=255)
                                                                                                                                            gcggGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCt           >  2:1583574/1‑150 (MQ=255)
                                                                                                                                              ggggATTCATGGTTGAAGATAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGc         >  2:1522241/1‑150 (MQ=255)
                                                                                                                                                ggATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAc       >  2:2302802/1‑150 (MQ=255)
                                                                                                                                                ggATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAc       <  2:3555269/150‑1 (MQ=255)
                                                                                                                                                 gATTAATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCAcc      >  1:3215789/1‑150 (MQ=255)
                                                                                                                                                  aTTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCa     <  1:2301408/150‑1 (MQ=255)
                                                                                                                                                  aTTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCa     <  1:2301243/150‑1 (MQ=255)
                                                                                                                                                   ttCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAg    <  1:2695514/150‑1 (MQ=255)
                                                                                                                                                   ttCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAg    <  1:3896973/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGCATTTTTACGTAGTCTTTGGCGTGGCCCCAGTCACGCAGGGAATCCATATTGCCGAGGTACAGGCACGACTCCAGCCCCTGGGCGATGTTGGCGATTGCGCGGGTGATTTTGCGGGTAACGAAGGTTTCGCCGCGGCGCGGGGATTCATGGTTGAAGAGAATTCCGTTACAGGCGTACATGCCGTAGGATTCACGGTAGTTAACGGTGATCCAGTAGGCGTACAGTTTGGCGACCGCATACGGAGATCGCGGGTAGAACGGCGTGGTCTCTTTCTGCGGAATTTCCTGCACCAGAC  >  CP009273/2121085‑2121383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑