Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,139,910 G→T 100% R1055L (CGA→CTA)  yegE → putative diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,139,9100GT99.3% 1030.4 / ‑1.6 292R1055L (CGA→CTA) yegEputative diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (159/131);  total (159/133)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 292 total aligned reads displayed.

CAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGA  >  CP009273/2139765‑2140056
                                                                                                                                                 |                                                                                                                                                  
cAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTc                                                                                                                                                >  2:3503852/1‑150 (MQ=255)
 aGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCg                                                                                                                                               <  2:4043906/150‑1 (MQ=255)
      ggCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATg                                                                                                                                          <  2:3449548/150‑1 (MQ=255)
      ggCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATg                                                                                                                                          <  2:1287036/150‑1 (MQ=255)
             aTCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCa                                                                                                                                   >  2:3770568/1‑150 (MQ=255)
              tCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCAt                                                                                                                                  >  2:3898400/1‑150 (MQ=255)
               cTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATc                                                                                                                                 >  1:3420492/1‑150 (MQ=255)
               cTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATc                                                                                                                                 <  2:3286309/150‑1 (MQ=255)
               cTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATc                                                                                                                                 <  2:3373960/150‑1 (MQ=255)
                 gCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGc                                                                                                                               >  1:1240193/1‑150 (MQ=255)
                  cAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGcc                                                                                                                              <  1:2908101/150‑1 (MQ=255)
                    aaTCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCgg                                                                                                                            <  2:1116831/150‑1 (MQ=255)
                         tCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAg                                                                                                                       >  2:3722779/1‑150 (MQ=255)
                          cAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGt                                                                                                                      <  1:949817/150‑1 (MQ=255)
                           aaCTCGCTGAAAGCGAATATGGCAGATTACCTGCTCCTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTc                                                                                                                     >  2:1610428/1‑150 (MQ=255)
                           aaCTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTc                                                                                                                     >  2:2769022/1‑150 (MQ=255)
                               cGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGttt                                                                                                                 <  2:1882325/150‑1 (MQ=255)
                                gCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGtttt                                                                                                                <  1:4028750/150‑1 (MQ=255)
                                gCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGtttt                                                                                                                >  2:3258804/1‑150 (MQ=255)
                                gCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGtttt                                                                                                                <  1:82047/150‑1 (MQ=255)
                                 cTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTa                                                                                                               >  2:1985088/1‑150 (MQ=255)
                                  tGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAc                                                                                                              >  2:2990478/1‑150 (MQ=255)
                                  tGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAc                                                                                                              >  1:137908/1‑150 (MQ=255)
                                  tGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTATAc                                                                                                              >  2:59372/1‑150 (MQ=255)
                                   gAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAcc                                                                                                             >  1:744262/1‑150 (MQ=255)
                                   gAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAcc                                                                                                             >  1:616742/1‑150 (MQ=255)
                                   gAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAcc                                                                                                             <  1:2643657/150‑1 (MQ=255)
                                     aaGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCt                                                                                                           >  1:3780023/1‑150 (MQ=255)
                                         gAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGt                                                                                                       >  1:83377/1‑150 (MQ=255)
                                           atatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGa                                                                                                     <  1:279554/150‑1 (MQ=255)
                                            tatGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAt                                                                                                    <  2:322306/150‑1 (MQ=255)
                                             atGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTAACCTTAGTGATg                                                                                                   >  2:2672087/1‑150 (MQ=255)
                                               ggCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGTTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACACTTAGTGATGGa                                                                                                 >  1:1343326/1‑150 (MQ=255)
                                                 cAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATa                                                                                               >  2:1266994/1‑150 (MQ=255)
                                                         cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCt                                                                                       <  1:2769475/150‑1 (MQ=255)
                                                         cTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGAAGGATACGCTTTCt                                                                                       >  2:3245487/1‑150 (MQ=255)
                                                          tGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTg                                                                                      <  2:4069231/150‑1 (MQ=255)
                                                          tGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTg                                                                                      >  2:587547/1‑150 (MQ=255)
                                                           gCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTgg                                                                                     <  1:535868/150‑1 (MQ=255)
                                                            ctactaGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGGTATGGATACGCtttaaggc                                                                                    >  1:702209/1‑145 (MQ=255)
                                                            cTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGc                                                                                    <  2:943211/150‑1 (MQ=255)
                                                            cTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGc                                                                                    >  1:1084459/1‑150 (MQ=255)
                                                              aCTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCAt                                                                                  <  2:2283666/150‑1 (MQ=255)
                                                               cTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATc                                                                                 >  2:506794/1‑150 (MQ=255)
                                                                 tGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCgg                                                                               >  1:264676/1‑150 (MQ=255)
                                                                    tGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGt                                                                            <  1:3109254/150‑1 (MQ=255)
                                                                           ttATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTg                                                                     >  1:2477673/1‑150 (MQ=255)
                                                                            tATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGa                                                                    >  1:1466607/1‑150 (MQ=255)
                                                                                   cAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATgg                                                             <  1:2376477/150‑1 (MQ=255)
                                                                                    atcGTGCAGGGTTATTTGATGGATGAGATGCTGATTTCGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                            <  1:1536737/148‑1 (MQ=255)
                                                                                    aaCGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggt                                                            >  1:3125129/1‑150 (MQ=255)
                                                                                      cGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtga                                                          <  2:2641246/150‑1 (MQ=255)
                                                                                        tGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATGTGATTTATggtgagg                                                        >  2:3932654/1‑150 (MQ=255)
                                                                                         gCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggt                                                       <  2:2479341/150‑1 (MQ=255)
                                                                                          cAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtg                                                      <  2:3872946/150‑1 (MQ=255)
                                                                                          cAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATggtgaggtg                                                      >  1:2118016/1‑150 (MQ=255)
                                                                                             ggTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGAtt                                                   <  1:1006940/150‑1 (MQ=255)
                                                                                              gTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTg                                                  <  1:2242436/150‑1 (MQ=255)
                                                                                               tAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGc                                                 >  2:4077885/1‑150 (MQ=255)
                                                                                                    tGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATg                                            <  1:2311849/150‑1 (MQ=255)
                                                                                                     gatggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGc                                           <  2:2177845/150‑1 (MQ=255)
                                                                                                      atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                          >  1:564837/1‑150 (MQ=255)
                                                                                                      atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                          <  1:1803748/150‑1 (MQ=255)
                                                                                                      atggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGcc                                          >  2:517057/1‑150 (MQ=255)
                                                                                                       tggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGGGAGGTGATTGCCGATGccc                                         >  1:3847112/1‑150 (MQ=255)
                                                                                                        ggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCa                                        >  2:1303824/1‑150 (MQ=255)
                                                                                                        ggatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATATGATTTATTGTGAGGTGATTGCCGATGCCCa                                        >  2:1787958/1‑150 (MQ=255)
                                                                                                         gatgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCaa                                       <  2:1491397/150‑1 (MQ=255)
                                                                                                          atgAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAAc                                      <  1:1492760/150‑1 (MQ=255)
                                                                                                              gaTGCTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCt                                  >  1:3078497/1‑150 (MQ=255)
                                                                                                                  cTGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATAAGCATTATGGCATCGGCGACGATAGGATTTATGGTGAGGTGATTGACGATGCCCAACCGCTGGAt                              >  2:1051801/1‑150 (MQ=255)
                                                                                                                   tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTATTGATGGATACGCTTTCAGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                             >  1:2089178/1‑150 (MQ=255)
                                                                                                                   tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAtt                             <  1:2519365/150‑1 (MQ=255)
                                                                                                                   tGATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGACCAACCGCTGGAtt                             <  1:2935132/150‑1 (MQ=255)
                                                                                                                    gATTTCGTTTATTCAGGGGCTCGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATTCGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGtttt                            <  1:3493754/150‑1 (MQ=255)
                                                                                                                    gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                            <  1:774004/150‑1 (MQ=255)
                                                                                                                    gATTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGAttt                            <  1:2697288/150‑1 (MQ=255)
                                                                                                                     aTTACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGGGAGGTGATTGCCGTTGCCCAACCGCTGGATTTg                           >  1:3574187/1‑150 (MQ=255)
                                                                                                                      ttACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGc                          >  1:3214980/1‑150 (MQ=255)
                                                                                                                       tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                         <  1:2445087/150‑1 (MQ=255)
                                                                                                                       tACGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCt                         <  1:3668907/150‑1 (MQ=255)
                                                                                                                         cGATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTgg                       >  1:939015/1‑150 (MQ=255)
                                                                                                                          gATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGt                      <  1:3819063/150‑1 (MQ=255)
                                                                                                                          gATTATTCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGt                      <  2:1413496/150‑1 (MQ=255)
                                                                                                                           attattCAGGGGCCCGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTg                     <  2:796828/150‑1 (MQ=255)
                                                                                                                           attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTg                     >  2:3089699/1‑150 (MQ=255)
                                                                                                                           attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTg                     <  1:4046125/150‑1 (MQ=255)
                                                                                                                           attattCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCGTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTg                     >  2:359395/1‑150 (MQ=255)
                                                                                                                           attagtCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTg                     <  2:7564/150‑1 (MQ=255)
                                                                                                                              attCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAt                  >  2:1202195/1‑150 (MQ=255)
                                                                                                                              attCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAt                  >  2:3354515/1‑150 (MQ=255)
                                                                                                                               ttCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAta                 >  1:2032668/1‑150 (MQ=255)
                                                                                                                               ttCAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAta                 >  1:3057593/1‑150 (MQ=255)
                                                                                                                                 cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagt               <  2:672122/150‑1 (MQ=255)
                                                                                                                                 cAGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagt               <  2:2014294/150‑1 (MQ=255)
                                                                                                                                  aGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagta              <  1:2108905/150‑1 (MQ=255)
                                                                                                                                  aGGGGCACGCTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAAtagta              >  2:3660267/1‑150 (MQ=255)
                                                                                                                                           cTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcg     <  1:3501169/150‑1 (MQ=255)
                                                                                                                                           cTCAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcg     <  1:3278914/150‑1 (MQ=255)
                                                                                                                                             cAGCTACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTcgcg   <  2:1451723/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CAGGTGGGCCGCGATCTGCAAATCTTCAACTCGCTGAAAGCGAATATGGCAGATTACCTGCTACTTGATGGTGAGTTATGCGCCAACGTGCAGGGTAATTTGATGGATGAGATGCTGATTACGATTATTCAGGGGCACGCTCAGCGACTCGGGATGAAAACCATCGCCGGGCCAGTCGTTTTACCCTTAGTGATGGATACGCTTTCTGGCATCGGCGTCGATCTGATTTATGGTGAGGTGATTGCCGATGCCCAACCGCTGGATTTGCTGGTGAATAGTAGTTATTTCGCGA  >  CP009273/2139765‑2140056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑