Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,220,503 C→T 34.9% V83M (GTG→ATG)  yohF ← putative oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,220,5030CT34.9% 299.4 / 248.8 232V83M (GTG→ATG) yohFputative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (85/66);  new base T (43/38);  total (128/104)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 234 total aligned reads displayed.

CCTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTG  >  CP009273/2220356‑2220650
                                                                                                                                                   |                                                                                                                                                   
cctgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACAt                                                                                                                                                   >  1:2360404/1‑150 (MQ=255)
 ctgccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATc                                                                                                                                                  >  1:2612935/1‑150 (MQ=255)
   gccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCaa                                                                                                                                                >  1:3782608/1‑150 (MQ=255)
   gccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCaa                                                                                                                                                >  1:1768785/1‑150 (MQ=255)
    ccctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAAt                                                                                                                                               <  2:4075826/150‑1 (MQ=255)
     cctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATg                                                                                                                                              <  1:2555208/150‑1 (MQ=255)
      ctTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATgc                                                                                                                                             >  1:4098405/1‑150 (MQ=255)
       ttGTTTCACCATCTGACGAGCCGCTATTTGCGAGCATAAGAATGCACCATCGACATCAACTGTAAAAATCTTGCGCCACTCATCAAAATCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATgcg                                                                                                                                            <  2:104520/150‑1 (MQ=255)
       ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATgcg                                                                                                                                            >  2:3599368/1‑150 (MQ=255)
       ttGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATgcg                                                                                                                                            >  2:772898/1‑150 (MQ=255)
         gTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGcc                                                                                                                                          <  1:3658326/150‑1 (MQ=255)
          tttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGccc                                                                                                                                         >  1:3269719/1‑150 (MQ=255)
           ttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGcccc                                                                                                                                        >  2:3874628/1‑150 (MQ=255)
           ttCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGcccc                                                                                                                                        >  2:2205308/1‑150 (MQ=255)
              aCCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGc                                                                                                                                     >  2:839608/1‑150 (MQ=255)
              aCCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCAAATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGc                                                                                                                                     >  1:1408226/1‑150 (MQ=255)
               ccATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGcc                                                                                                                                    <  1:2528445/150‑1 (MQ=255)
                 aTCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGt                                                                                                                                  <  1:3575716/150‑1 (MQ=255)
                 aTCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGt                                                                                                                                  <  2:3574904/150‑1 (MQ=255)
                   cTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTg                                                                                                                                >  2:2648829/1‑150 (MQ=255)
                     gACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGaa                                                                                                                              <  1:595675/150‑1 (MQ=255)
                     gACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGaa                                                                                                                              >  1:1829370/1‑150 (MQ=255)
                         aGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAg                                                                                                                          >  2:2788574/1‑150 (MQ=255)
                          gCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         >  2:1393839/1‑150 (MQ=255)
                          gCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         >  2:394180/1‑150 (MQ=255)
                          gCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGt                                                                                                                         <  2:3542279/150‑1 (MQ=255)
                            cGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGttt                                                                                                                       <  1:1723261/150‑1 (MQ=255)
                              cAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     <  1:1025073/150‑1 (MQ=255)
                              cAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     >  2:2387259/1‑150 (MQ=255)
                              cAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTtct                                                                                                                     <  1:1275469/150‑1 (MQ=255)
                                aTTTGCGAGCATCAGAATGCACCAGCGACATCACCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTcc                                                                                                                   <  1:55469/150‑1 (MQ=255)
                                 tttGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCa                                                                                                                  <  1:2925382/150‑1 (MQ=255)
                                        gCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAg                                                                                                           >  2:1671723/1‑150 (MQ=255)
                                              gAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGcccc                                                                                                     <  1:1232298/150‑1 (MQ=255)
                                                aTGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCtt                                                                                                   >  2:2524031/1‑150 (MQ=255)
                                                  gCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCt                                                                                                 <  1:671022/150‑1 (MQ=255)
                                                  gCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCt                                                                                                 >  1:3215337/1‑150 (MQ=255)
                                                   cACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTg                                                                                                <  1:2721291/150‑1 (MQ=255)
                                                    aCCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTgg                                                                                               <  1:371909/150‑1 (MQ=255)
                                                     ccATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGt                                                                                              >  1:1588892/1‑150 (MQ=255)
                                                       aTCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAg                                                                                            <  2:1848235/150‑1 (MQ=255)
                                                         cGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAt                                                                                          <  2:302333/150‑1 (MQ=255)
                                                          gACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                         <  2:3289338/150‑1 (MQ=255)
                                                          gACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGAtt                                                                                         >  1:922592/1‑150 (MQ=255)
                                                            cATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGACTGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGc                                                                                       >  2:1409961/1‑150 (MQ=255)
                                                            cATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGc                                                                                       <  1:416738/150‑1 (MQ=255)
                                                            cATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGc                                                                                       >  2:3381559/1‑150 (MQ=255)
                                                             aTCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGcc                                                                                      >  1:3558810/1‑150 (MQ=255)
                                                             aTCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGcc                                                                                      <  1:3459528/150‑1 (MQ=255)
                                                                aaCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCgag                                                                                   <  2:1952153/150‑1 (MQ=255)
                                                                aaCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCgag                                                                                   >  1:876086/1‑150 (MQ=255)
                                                                aaCGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCAATGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCgag                                                                                   >  2:121465/1‑150 (MQ=255)
                                                                    gTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCAGTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATcc                                                                               <  2:652194/150‑1 (MQ=255)
                                                                      aaaaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAg                                                                             >  1:1017719/1‑150 (MQ=255)
                                                                        aaaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCt                                                                           <  2:719816/150‑1 (MQ=255)
                                                                         aaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTg                                                                          >  2:3272066/1‑150 (MQ=255)
                                                                         aaTCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTATGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTg                                                                          <  2:341577/150‑1 (MQ=255)
                                                                             ttGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACg                                                                      >  1:2761698/1‑150 (MQ=255)
                                                                              tGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGa                                                                     >  1:461881/1‑150 (MQ=255)
                                                                                cgcCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtc                                                                   >  2:2322828/1‑150 (MQ=255)
                                                                                  ccACTCATCAAAAGCCATATCACGAAACGGCGCTTTGGTCATTGCACCCGAATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtctc                                                                 <  1:92762/150‑1 (MQ=255)
                                                                                  ccACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGAtctc                                                                 >  1:3296182/1‑150 (MQ=255)
                                                                                     cTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGc                                                              >  1:1882961/1‑150 (MQ=255)
                                                                                      tcatcaAAAGCCTTATCATGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCTGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCa                                                             <  1:2405515/150‑1 (MQ=255)
                                                                                       catcaAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCAc                                                            >  1:3339871/1‑150 (MQ=255)
                                                                                        ataaaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACg                                                           >  2:3541853/4‑150 (MQ=255)
                                                                                           aaaaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTAc                                                        <  2:2235899/150‑1 (MQ=255)
                                                                                             aaGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGc                                                      >  2:2115009/1‑150 (MQ=255)
                                                                                              aGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGcc                                                     <  2:110777/150‑1 (MQ=255)
                                                                                                  atatCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTgg                                                 <  1:3208011/150‑1 (MQ=255)
                                                                                                   tatCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGc                                                <  1:1619869/150‑1 (MQ=255)
                                                                                                     tCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTa                                              <  2:507561/150‑1 (MQ=255)
                                                                                                        aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACt                                           >  2:1797383/1‑150 (MQ=255)
                                                                                                        aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACt                                           <  1:2093214/150‑1 (MQ=255)
                                                                                                        aGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACt                                           >  2:718272/1‑150 (MQ=255)
                                                                                                          aaaCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTAc                                         >  1:2443742/1‑150 (MQ=255)
                                                                                                            aCGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCt                                       <  2:1875121/150‑1 (MQ=255)
                                                                                                             cGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTc                                      >  1:2696314/1‑150 (MQ=255)
                                                                                                                         cATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATc                          >  2:2901543/1‑150 (MQ=255)
                                                                                                                          aTTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                         <  1:772890/150‑1 (MQ=255)
                                                                                                                          aTTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCt                         <  2:2437731/150‑1 (MQ=255)
                                                                                                                           ttGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtt                        <  1:2161449/150‑1 (MQ=255)
                                                                                                                             gCACCCGCATTATTCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtttt                      <  1:1738809/150‑1 (MQ=255)
                                                                                                                             gCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtttt                      <  2:893664/150‑1 (MQ=255)
                                                                                                                             gCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCtttt                      <  2:705392/150‑1 (MQ=255)
                                                                                                                                  cGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGcccc                 <  1:980091/150‑1 (MQ=255)
                                                                                                                                   gCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCct                >  2:1706156/1‑150 (MQ=255)
                                                                                                                                     attattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttc              >  1:190090/1‑150 (MQ=255)
                                                                                                                                     attattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttc              >  1:1170352/1‑150 (MQ=255)
                                                                                                                                      ttataCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCACACGCGCGGTATCTATTGCCCcttct             >  1:1479640/1‑150 (MQ=255)
                                                                                                                                       tattCACCAGCATATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt            <  2:1718118/150‑1 (MQ=255)
                                                                                                                                       tattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt            <  1:315862/150‑1 (MQ=255)
                                                                                                                                       tattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt            <  1:77436/150‑1 (MQ=255)
                                                                                                                                       tattCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCcttctt            <  1:2483318/150‑1 (MQ=255)
                                                                                                                                        attCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCttcttc           >  2:1917427/1‑150 (MQ=255)
                                                                                                                                           cACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATc        >  1:38013/1‑150 (MQ=255)
                                                                                                                                            aCCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCt       >  2:2833664/1‑150 (MQ=255)
                                                                                                                                              cAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGa     >  2:2117694/1‑150 (MQ=255)
                                                                                                                                               aGCACATCAATGCGCCCCAGCCGTTGAATGATTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAg    >  1:3094338/1‑150 (MQ=255)
                                                                                                                                               aGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAg    >  2:3606716/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCTGCCCTTGTTTCACCATCTGACGAGCCGCAATTTGCGAGCATAAGAATGCACCATCGACATCAACGGTAAAAATCTTGCGCCACTCATCAAAAGCCATATCAAGAAACGGCGCTTTGGTCATTGCACCCGCATTATTCACCAGCACATCAATGCGCCCCAGCCGTTGAATGAGTTTCTCCAGCGCCAGTGCCCCTTCTGGTAGATTGCCGAGATCCAGCTGCACGATCTCCGCACGTACGCCGTGGCTAACTACCTCACGCGCGGTATCTTTTGCCCCTTCTTCATCTGAGTG  >  CP009273/2220356‑2220650

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑