Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,225,190 C→T 100% G231G (GGC→GGT)  yohK → LrgB family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,225,1900CT97.4% 822.3 / 7.1 232G231G (GGC→GGT) yohKLrgB family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base T (104/122);  total (106/126)
Fisher's exact test for biased strand distribution p-value = 6.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

GACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225043‑2225336
                                                                                                                                                   |                                                                                                                                                  
gACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTa                                                                                                                                                  <  2:2709377/150‑1 (MQ=255)
gACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTa                                                                                                                                                  <  1:618513/150‑1 (MQ=255)
  cTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaa                                                                                                                                                <  1:2134112/150‑1 (MQ=255)
   tGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTaaaa                                                                                                                                               >  2:902917/1‑150 (MQ=255)
     ccTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAAtt                                                                                                                                             >  2:2966669/1‑150 (MQ=255)
      cTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAAttt                                                                                                                                            <  1:582018/150‑1 (MQ=255)
      cTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAAttt                                                                                                                                            <  2:793844/150‑1 (MQ=255)
         gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCg                                                                                                                                         >  1:975203/1‑150 (MQ=255)
         gCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCg                                                                                                                                         <  2:1164386/150‑1 (MQ=255)
           aCGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAAGGGGTTAAAATTTGCGAt                                                                                                                                       >  1:2868941/1‑150 (MQ=255)
            cGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATg                                                                                                                                      <  1:3697206/150‑1 (MQ=255)
                     tACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCa                                                                                                                             <  2:901196/150‑1 (MQ=255)
                       cGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAtt                                                                                                                           <  1:178521/150‑1 (MQ=255)
                        ggCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttt                                                                                                                          >  1:2913894/1‑150 (MQ=255)
                         gcgcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAtttt                                                                                                                         >  1:3337282/1‑150 (MQ=255)
                          cgcgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCAttttt                                                                                                                        <  2:1043026/150‑1 (MQ=255)
                            cgcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGa                                                                                                                      <  2:2338719/150‑1 (MQ=255)
                             gcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                     >  1:3718316/1‑150 (MQ=255)
                             gcTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGat                                                                                                                     >  1:3718211/1‑150 (MQ=255)
                                gcgcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgta                                                                                                                  <  1:1467960/150‑1 (MQ=255)
                                  gcCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAAAGTGTTAAAATTTGCGATGCGTCGCGCATTTTTGatgtatc                                                                                                                >  2:2937831/1‑149 (MQ=255)
                                     gAGCTGGATTATCAGGAAGGTGCTTTTAGTTCGCTAGCGATGGTGTTATGCGGGATAATTACTTCGCTGTTCGCACCGTTCCTTTTCCCGATTATTCTGGCTGTAATGGGTTATAATTTGCGATGCGTCGCGCATTTTTGATGTATGttt                                                                                                             <  2:656249/150‑1 (MQ=255)
                                       gCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCa                                                                                                           <  2:2446586/150‑1 (MQ=255)
                                       gCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCa                                                                                                           >  1:2691890/1‑150 (MQ=255)
                                         tGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcg                                                                                                         >  2:3253432/1‑150 (MQ=255)
                                           gATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                       >  1:1415138/1‑150 (MQ=255)
                                           gATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCAcgcg                                                                                                       <  1:2630343/150‑1 (MQ=255)
                                             ttATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGtt                                                                                                     <  1:2229806/150‑1 (MQ=255)
                                              tATCAGTAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCTCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTg                                                                                                    >  1:3655488/1‑150 (MQ=255)
                                               aTCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAAGTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGc                                                                                                   <  2:3525870/150‑1 (MQ=255)
                                                     aaGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatt                                                                                             <  2:3218025/150‑1 (MQ=255)
                                                      aGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatta                                                                                            >  1:2579131/1‑150 (MQ=255)
                                                      aGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaatta                                                                                            <  1:1500834/150‑1 (MQ=255)
                                                       ggTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaa                                                                                           <  1:3083571/150‑1 (MQ=255)
                                                        gTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCAtaattaat                                                                                          <  1:2368391/150‑1 (MQ=255)
                                                            aTTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgaga                                                                                      >  2:2601086/1‑150 (MQ=255)
                                                            aTTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATgaga                                                                                      <  1:4160015/150‑1 (MQ=255)
                                                             tttAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAt                                                                                     <  1:790012/150‑1 (MQ=255)
                                                              ttAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGAtt                                                                                    <  2:3542998/150‑1 (MQ=255)
                                                                   tCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGAt                                                                               >  1:909095/1‑150 (MQ=255)
                                                                     gCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATca                                                                             >  2:204212/1‑150 (MQ=255)
                                                                      cTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATcac                                                                            <  2:2644355/150‑1 (MQ=255)
                                                                       tAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATcaca                                                                           >  1:71758/1‑150 (MQ=255)
                                                                          cgcTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACatat                                                                        >  2:3767604/1‑150 (MQ=255)
                                                                            cTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaa                                                                      >  2:1119433/1‑150 (MQ=255)
                                                                             tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                     <  2:2624437/150‑1 (MQ=255)
                                                                             tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                     >  2:221393/1‑150 (MQ=255)
                                                                             tGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATaaa                                                                     <  2:3827027/150‑1 (MQ=255)
                                                                                 gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                 <  2:3160120/150‑1 (MQ=255)
                                                                                 gtTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCca                                                                 <  2:2755678/150‑1 (MQ=255)
                                                                                  ttATGAGTGATAATTTCTTCGCTGATCGCACCGTTCCTTTTCCCGATTTTTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCcac                                                                <  1:3410490/150‑1 (MQ=255)
                                                                                    aTGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTCTTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              >  2:101131/1‑150 (MQ=255)
                                                                                    aTGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              >  1:1043242/1‑150 (MQ=255)
                                                                                    aTGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACaa                                                              >  2:32889/1‑150 (MQ=255)
                                                                                     tGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                             <  1:4072022/150‑1 (MQ=255)
                                                                                     tGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAAc                                                             >  1:40103/1‑150 (MQ=255)
                                                                                        gggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACggg                                                          <  1:1589525/150‑1 (MQ=255)
                                                                                         ggATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGt                                                         >  2:701174/1‑150 (MQ=255)
                                                                                          gATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGtt                                                        <  2:1912363/150‑1 (MQ=255)
                                                                                           aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  2:1769901/150‑1 (MQ=255)
                                                                                           aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  1:466529/150‑1 (MQ=255)
                                                                                           aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  2:3991220/150‑1 (MQ=255)
                                                                                           aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  2:1245087/150‑1 (MQ=255)
                                                                                           aTAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTc                                                       <  1:4031439/150‑1 (MQ=255)
                                                                                               ttACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTaa                                                   <  2:564878/150‑1 (MQ=255)
                                                                                                 aCTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAAc                                                 >  2:490892/1‑150 (MQ=255)
                                                                                                     cGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGtt                                             <  2:468961/150‑1 (MQ=255)
                                                                                                        tGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATc                                          <  2:2476620/150‑1 (MQ=255)
                                                                                                         gATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATcc                                         <  2:2576607/150‑1 (MQ=255)
                                                                                                         gATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATcc                                         <  1:832675/150‑1 (MQ=255)
                                                                                                          aTCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATccc                                        <  2:4067885/150‑1 (MQ=255)
                                                                                                             gCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCAtt                                     >  1:1533723/1‑150 (MQ=255)
                                                                                                              cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    <  2:2740675/150‑1 (MQ=255)
                                                                                                              cACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTa                                    >  2:3496070/1‑150 (MQ=255)
                                                                                                               aCCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTAc                                   >  1:946593/1‑150 (MQ=255)
                                                                                                                  gTTCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATg                                >  1:67188/1‑150 (MQ=255)
                                                                                                                   ttCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGa                               >  2:9147/1‑150 (MQ=255)
                                                                                                                    tCCTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAt                              >  1:1166665/1‑150 (MQ=255)
                                                                                                                     ccTTTTCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGAtt                             >  2:310942/1‑150 (MQ=255)
                                                                                                                          tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                        >  2:18662/1‑150 (MQ=255)
                                                                                                                          tCCCGATTATTCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAg                        >  2:392389/1‑150 (MQ=255)
                                                                                                                             cGATTATTCTGGCAGGAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCa                     <  2:1271299/150‑1 (MQ=255)
                                                                                                                               attattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAAc                   <  1:817420/150‑1 (MQ=255)
                                                                                                                                 tattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc                 >  2:1615247/1‑150 (MQ=255)
                                                                                                                                 tattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc                 >  2:3556424/1‑150 (MQ=255)
                                                                                                                                 tattCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGc                 >  2:1407612/1‑150 (MQ=255)
                                                                                                                                  attCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGcc                >  2:2397162/1‑150 (MQ=255)
                                                                                                                                   ttCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCa               <  1:2411945/150‑1 (MQ=255)
                                                                                                                                    tCTGGCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCAt              <  2:1540236/150‑1 (MQ=255)
                                                                                                                                       ggCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa           >  2:520672/1‑150 (MQ=255)
                                                                                                                                       ggCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCa           <  1:301683/150‑1 (MQ=255)
                                                                                                                                        gCAGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCAt          >  2:4100834/1‑150 (MQ=255)
                                                                                                                                        gCAGTAATGGGTTAAAATTTGAGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCAt          >  1:2749614/1‑150 (MQ=255)
                                                                                                                                          aGTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATcc        <  1:1216112/150‑1 (MQ=255)
                                                                                                                                           gTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa       >  1:2268862/1‑150 (MQ=255)
                                                                                                                                           gTAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCa       >  2:2010451/1‑150 (MQ=255)
                                                                                                                                            tAATGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCAc      <  2:617774/150‑1 (MQ=255)
                                                                                                                                              aTGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGt    >  1:91609/1‑150 (MQ=255)
                                                                                                                                               tGGGTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGtt   >  1:1996733/1‑150 (MQ=255)
                                                                                                                                                gggTTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGttt  <  1:4124616/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GACTGCCTCGCACGCCCTCGGTACGGCGCGCTGCGCCGAGCTGGATTATCAGGAAGGTGCATTTAGTTCGCTAGCGCTGGTGTTATGCGGGATAATTACTTCGCTGATCGCACCGTTCCTTTTCCCGATTATTCTGGCAGTAATGGGCTAAAATTTGCGATGCGTCGCGCATTTTTGATGTATGTTTCACGCGTTGCATAATTAATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTT  >  CP009273/2225043‑2225336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑