BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,353,889	C→A	34.0%	V335V (GTG→GTT)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,353,889	0	C	A	34.0%	409.5 / 224.4	238	V335V (GTG→GTT)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand): ref base C (73/84); new base A (39/42); total (112/126)
Fisher's exact test for biased strand distribution p-value = 8.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

AAACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353740‑2354038

  aCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAc                                                                                                                                                     <  1:3464448/150‑1 (MQ=255)

  aCCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCAc                                                                                                                                                     >  1:3673249/1‑150 (MQ=255)

    cGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCg                                                                                                                                                   >  1:3569938/1‑150 (MQ=255)

    cGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCg                                                                                                                                                   >  2:2679181/1‑150 (MQ=255)

       gTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCAt                                                                                                                                                >  2:467707/1‑150 (MQ=255)

        tttATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATg                                                                                                                                               <  2:944529/150‑1 (MQ=255)

        ttaaTCAAGAACTGATTTATGAATACGACCATTCACCGGAAACGGAACAAAGAGCATAATCGGGACAAACTTCGGACGCATCGTTGAAAAAACAGGGCTGGTCATCAGGAAATCGCTTAATGTCGTATTGGTGAATGTGATAAACGCATg                                                                                                                                               >  2:153447/4‑150 (MQ=255)

         ttATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATgg                                                                                                                                              >  1:2086163/1‑150 (MQ=255)

         ttATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATgg                                                                                                                                              <  2:3358584/150‑1 (MQ=255)

          tATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGt                                                                                                                                             >  2:434409/1‑150 (MQ=255)

           aTCAAGCTCTGATTTATGAATTCGACCATTCTCCGGAACCGGCTCATTGAGCAGACTCGGGTCTAACTGCGGACGCATCGTTGTACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTg                                                                                                                                            <  1:3116368/150‑1 (MQ=255)

           aTCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTg                                                                                                                                            <  1:1550787/150‑1 (MQ=255)

              aaGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgaga                                                                                                                                         >  2:3541669/1‑150 (MQ=255)

              aaGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTgaga                                                                                                                                         >  1:129430/1‑150 (MQ=255)

                gCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGt                                                                                                                                       <  1:2443689/150‑1 (MQ=255)

                  aCTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTaa                                                                                                                                     >  1:952727/1‑150 (MQ=255)

                      aTTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGACTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAg                                                                                                                                 >  2:3979543/1‑150 (MQ=255)

                       tttATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGa                                                                                                                                >  1:300996/1‑150 (MQ=255)

                         tATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACg                                                                                                                              >  2:169398/1‑150 (MQ=255)

                             aaTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAAcc                                                                                                                          <  2:3278841/150‑1 (MQ=255)

                              aTACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCg                                                                                                                         <  2:214307/150‑1 (MQ=255)

                                 cGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGc                                                                                                                      >  2:2071384/1‑150 (MQ=255)

                                    ccATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGc                                                                                                                   >  2:2038565/1‑150 (MQ=255)

                                     cATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGca                                                                                                                  >  1:3176075/1‑150 (MQ=255)

                                     cATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGca                                                                                                                  <  1:832115/150‑1 (MQ=255)

                                      aTTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcac                                                                                                                 >  1:1072495/1‑150 (MQ=255)

                                        tCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcacct                                                                                                               <  2:1668893/150‑2 (MQ=255)

                                         cACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcaccac                                                                                                              >  2:753643/1‑150 (MQ=255)

                                         cACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGcaccac                                                                                                              >  2:136068/1‑150 (MQ=255)

                                            cGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAg                                                                                                           >  2:2280448/1‑150 (MQ=255)

                                            cGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAg                                                                                                           <  1:3385380/150‑1 (MQ=255)

                                              gAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGtt                                                                                                         >  2:399096/1‑150 (MQ=255)

                                               aaCCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTg                                                                                                        >  2:1469272/1‑150 (MQ=255)

                                                 ccGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGcc                                                                                                      <  2:720337/150‑1 (MQ=255)

                                                  cGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccc                                                                                                     <  1:417837/150‑1 (MQ=255)

                                                    gCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGccccc                                                                                                   <  1:1767801/150‑1 (MQ=255)

                                                     cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                  <  1:672202/150‑1 (MQ=255)

                                                     cTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCt                                                                                                  <  2:3040210/150‑1 (MQ=255)

                                                        aTTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGa                                                                                               >  2:4066159/1‑150 (MQ=255)

                                                             gCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTgg                                                                                          <  2:2534170/150‑1 (MQ=255)

                                                              cAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGc                                                                                         >  1:1689099/1‑150 (MQ=255)

                                                              cAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGc                                                                                         <  1:2555383/150‑1 (MQ=255)

                                                                  aTCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGAtt                                                                                     <  2:838520/150‑1 (MQ=255)

                                                                       gTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGc                                                                                <  2:306227/150‑1 (MQ=255)

                                                                          aaaCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAt                                                                             >  2:3668732/1‑150 (MQ=255)

                                                                            aCTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATATGGCGATTGCGGCAAttt                                                                           >  2:844721/1‑150 (MQ=255)

                                                                                cGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccac                                                                       <  1:3541410/150‑1 (MQ=255)

                                                                                  gACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacca                                                                     >  2:479354/1‑150 (MQ=255)

                                                                                  gACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTccacca                                                                     <  1:4047839/150‑1 (MQ=255)

                                                                                     gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCg                                                                  >  1:3363972/1‑150 (MQ=255)

                                                                                     gCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCg                                                                  <  2:1201797/150‑1 (MQ=255)

                                                                                         cGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGt                                                              <  2:2911382/150‑1 (MQ=255)

                                                                                          gTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGtt                                                             >  2:2458570/1‑150 (MQ=255)

                                                                                            tGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTaa                                                           >  1:1901428/1‑150 (MQ=255)

                                                                                            tGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTaa                                                           >  2:2065318/1‑150 (MQ=255)

                                                                                              aaCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAac                                                         >  2:1578352/1‑150 (MQ=255)

                                                                                              aaCAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAac                                                         >  1:3698311/1‑150 (MQ=255)

                                                                                                  aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                     >  2:1110070/1‑150 (MQ=255)

                                                                                                  aTCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAaccacc                                                     <  2:523917/150‑1 (MQ=255)

                                                                                                         cTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAg                                              <  1:3104982/150‑1 (MQ=255)

                                                                                                           ggTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGcc                                            >  2:1683533/1‑150 (MQ=255)

                                                                                                            gTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCa                                           <  2:1036039/150‑1 (MQ=255)

                                                                                                            gTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCa                                           <  1:732385/150‑1 (MQ=255)

                                                                                                              catcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAAc                                         <  1:1802950/150‑1 (MQ=255)

                                                                                                              catcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAAc                                         <  2:2643326/150‑1 (MQ=255)

                                                                                                               atcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACa                                        >  1:3839761/1‑150 (MQ=255)

                                                                                                                tcaGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACAt                                       <  2:2543390/150‑1 (MQ=255)

                                                                                                                  aGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATcc                                     <  2:3988449/150‑1 (MQ=255)

                                                                                                                  aGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGATGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATcc                                     >  1:1593709/1‑150 (MQ=255)

                                                                                                                   ggAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCg                                    <  1:2035194/150‑1 (MQ=255)

                                                                                                                     aaTTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGc                                  >  1:633752/1‑150 (MQ=255)

                                                                                                                        tCGCTGAATGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGc                               <  2:2791579/150‑1 (MQ=255)

                                                                                                                          gCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCAt                             <  2:909520/150‑1 (MQ=255)

                                                                                                                           cTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATa                            >  2:4137146/1‑150 (MQ=255)

                                                                                                                            tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATaa                           >  1:1294113/1‑150 (MQ=255)

                                                                                                                            tGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGAGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATaa                           <  1:467707/150‑1 (MQ=255)

                                                                                                                             gAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAAt                          >  1:3329890/1‑150 (MQ=255)

                                                                                                                              aaTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATg                         <  2:300996/150‑1 (MQ=255)

                                                                                                                               aTGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGt                        <  2:2611736/150‑1 (MQ=255)

                                                                                                                                tGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       <  2:3883549/150‑1 (MQ=255)

                                                                                                                                tGGCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTc                       >  1:3225832/1‑150 (MQ=255)

                                                                                                                                 ggCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCg                      <  2:3173530/150‑1 (MQ=255)

                                                                                                                                 ggCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCg                      <  2:110595/150‑1 (MQ=255)

                                                                                                                                  gCGTATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCCCCAGTTGCCCACTGGATTTGGCGATTGCTGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGa                     <  2:1878155/150‑1 (MQ=255)

                                                                                                                                    gTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATa                   <  2:1287308/150‑1 (MQ=255)

                                                                                                                                     tATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAc                  <  1:2729445/150‑1 (MQ=255)

                                                                                                                                     tATTGGTGAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAc                  <  1:2912040/150‑1 (MQ=255)

                                                                                                                                      aTTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATAcc                 <  1:2716752/150‑1 (MQ=255)

                                                                                                                                         ggTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGt              >  1:2909410/1‑150 (MQ=255)

                                                                                                                                           tGAAGGTGATCACCGCGTGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGttt            <  2:635468/150‑1 (MQ=255)

                                                                                                                                           tGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAAGTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGttt            <  2:2846200/150‑1 (MQ=255)

                                                                                                                                            gAAGGTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTc           >  1:2822158/1‑150 (MQ=255)

                                                                                                                                             aaGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCt          <  2:1952777/150‑1 (MQ=255)

                                                                                                                                               ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGa        <  1:2574414/150‑1 (MQ=255)

                                                                                                                                               ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGa        <  1:2966302/150‑1 (MQ=255)

                                                                                                                                               ggTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGa        >  1:1819851/1‑150 (MQ=255)

                                                                                                                                                gTGATAACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGaa       >  2:3773988/1‑150 (MQ=255)

                                                                                                                                                  gATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAg     >  1:442462/1‑150 (MQ=255)

                                                                                                                                                   aTCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGc    >  2:777756/1‑150 (MQ=255)

                                                                                                                                                     cACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGt  <  1:3666684/150‑1 (MQ=255)

AAACCGGGTTTATCAAGCACTGATTTATGAATACGACCATTCACCGGAACCGGCTCATTGAGCAGAATCGGGTCAAACTGCGGACGCATCGTTGAACAATCCGGGCTGGTCATCAGGAATTCGCTGAATGGCGTATTGGTGAAGGTGATCACCGCATGGTGAGAGTAAACAGACGAACCGTGCGGCACCACCAGTTGCCCCCTGGATTTGGCGATTGCGGCAATTTCCACCAGCGTGGTTAAACCACCGCACCAGCCAACATCCGGCTGCATAATGTCGATACCGGTTTCTGAAAGCGT  >  CP009273/2353740‑2354038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`