BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	2,373,129	C→A	7.2%	D332Y (GAT→TAT)	menF ←	isochorismate synthase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,373,129	0	C	A	7.2%	962.8 / 33.2	249	D332Y (GAT→TAT)	menF	isochorismate synthase 2
Reads supporting (aligned to +/- strand): ref base C (105/126); new base A (8/10); total (113/136)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01

Only 100 of 262 total aligned reads displayed.

TTCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCT  >  CP009273/2372980‑2373278

ttCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCAcatc                                                                                                                                                       >  2:3325733/1‑150 (MQ=255)

   gCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACatcatc                                                                                                                                                    <  2:3687293/150‑1 (MQ=255)

   gCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACatcatc                                                                                                                                                    >  1:1879899/1‑150 (MQ=255)

         ttgtAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCttt                                                                                                                                              <  2:1193303/150‑1 (MQ=255)

            tAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTttgtt                                                                                                                                           >  1:300745/1‑150 (MQ=255)

                 agagaTAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTg                                                                                                                                      <  2:310929/150‑1 (MQ=255)

                       aGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTcc                                                                                                                                >  2:3137430/1‑150 (MQ=255)

                       aGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTcc                                                                                                                                >  2:3137402/1‑150 (MQ=255)

                       aGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTcc                                                                                                                                >  1:4152811/1‑150 (MQ=255)

                           cGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGAt                                                                                                                            <  2:3779265/150‑1 (MQ=255)

                             cAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATAc                                                                                                                          <  1:2811504/150‑1 (MQ=255)

                             cAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATAc                                                                                                                          <  1:589850/150‑1 (MQ=255)

                             cAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATAc                                                                                                                          >  1:641966/1‑150 (MQ=255)

                              aGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACa                                                                                                                         >  2:3890824/1‑150 (MQ=255)

                              aGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACa                                                                                                                         <  1:2062097/150‑1 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        <  2:1095535/150‑1 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  1:2887825/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  1:2797868/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  1:4170393/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  1:1139804/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  2:608542/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        >  2:608414/1‑150 (MQ=255)

                               gAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAg                                                                                                                        <  1:3598822/150‑1 (MQ=255)

                                aaCCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTGTGTTGAGTGAAGTCCAGATACAgc                                                                                                                       >  2:1876471/1‑150 (MQ=255)

                                aaCCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTGTGTTGAGTGAAGTCCAGATACAgc                                                                                                                       >  2:2266009/1‑150 (MQ=255)

                                   cGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcggc                                                                                                                    <  2:2939319/150‑1 (MQ=255)

                                   cGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAgcggc                                                                                                                    <  1:3276505/150‑1 (MQ=255)

                                   cGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTGTGTTGAGTGAAGTCCAGATACAgcggc                                                                                                                    >  2:2341940/1‑150 (MQ=255)

                                      cGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGaa                                                                                                                 <  2:2743626/150‑1 (MQ=255)

                                       gTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAg                                                                                                                <  2:624571/150‑1 (MQ=255)

                                            aTTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgct                                                                                                           <  1:1851484/150‑1 (MQ=255)

                                            aTTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgct                                                                                                           <  2:830925/150‑1 (MQ=255)

                                            aTTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgct                                                                                                           <  2:3457552/150‑1 (MQ=255)

                                              tctcGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGAtgctgc                                                                                                         >  2:982854/1‑150 (MQ=255)

                                                 cGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACt                                                                                                      >  2:1307584/1‑150 (MQ=255)

                                                   ggTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACttt                                                                                                    <  1:2196430/150‑1 (MQ=255)

                                                   ggTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACttt                                                                                                    <  1:1580809/150‑1 (MQ=255)

                                                    gTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTa                                                                                                   >  2:88933/1‑150 (MQ=255)

                                                          ggTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcaga                                                                                             >  2:2976721/1‑150 (MQ=255)

                                                           gTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagac                                                                                            <  2:572307/150‑1 (MQ=255)

                                                           gTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagac                                                                                            <  1:555947/150‑1 (MQ=255)

                                                           gTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagac                                                                                            <  2:2652922/150‑1 (MQ=255)

                                                             tCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTacgcagacgc                                                                                          >  1:2362263/1‑150 (MQ=255)

                                                              cGTGACGGGCGATAAACTGTCGCTCCAGATCGCGCTGTAAGCCAGCAACTGCTTCCGTTGGCTGCACCTGATTTAAACAGATCACATCATCCGCTTTTTTGAGTGAAGTCCAGATACATCGGCGAAGATGCTGCACTTTacgcagacgca                                                                                         <  1:10008/150‑1 (MQ=255)

                                                                  aCGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAc                                                                                     <  1:1458430/150‑1 (MQ=255)

                                                                   cGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAcc                                                                                    <  2:4127658/150‑1 (MQ=255)

                                                                   cGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTAcc                                                                                    <  1:2397353/150‑1 (MQ=255)

                                                                    gggCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCt                                                                                   <  2:1214654/150‑1 (MQ=255)

                                                                     ggCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTg                                                                                  <  1:1274437/150‑1 (MQ=255)

                                                                     ggCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTg                                                                                  <  2:3384756/150‑1 (MQ=255)

                                                                       cGATAATCTGTCGAGCCAGATCGCGCGGTAAGCCAGCTACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGAGACAGCGGCGAAGATGCTGCACTTTACGCTGACGCAGTACCTgcg                                                                                <  2:1774824/150‑1 (MQ=255)

                                                                         aTAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTgcggc                                                                              >  1:780843/1‑150 (MQ=255)

                                                                            aaCTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGt                                                                           <  1:324390/150‑1 (MQ=255)

                                                                              cTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTaa                                                                         <  2:1740499/150‑1 (MQ=255)

                                                                                 tCGCGCCAGATCGCGCGGTAAGCCAGCATCTGCTGCCGTTGGATGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAAc                                                                      <  1:2072911/150‑1 (MQ=255)

                                                                                      ccAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCa                                                                 >  1:921724/1‑150 (MQ=255)

                                                                                      ccAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCa                                                                 <  2:3766500/150‑1 (MQ=255)

                                                                                        aGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGc                                                               >  2:1379162/1‑150 (MQ=255)

                                                                                        aGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGc                                                               >  2:1379259/1‑150 (MQ=255)

                                                                                        aGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGc                                                               >  1:2980702/1‑150 (MQ=255)

                                                                                             gcgcgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTg                                                          <  1:1829059/150‑1 (MQ=255)

                                                                                                cgGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGt                                                       <  1:2011513/150‑1 (MQ=255)

                                                                                                 ggTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTCAAACATCCAGCGTCTGGGTa                                                      >  1:112557/1‑150 (MQ=255)

                                                                                                 ggTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTa                                                      <  2:491834/150‑1 (MQ=255)

                                                                                                  gTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTAt                                                     <  1:3921982/150‑1 (MQ=255)

                                                                                                  gTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTAt                                                     >  2:2884781/1‑150 (MQ=255)

                                                                                                   tAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATc                                                    >  1:1784621/1‑150 (MQ=255)

                                                                                                   tAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATc                                                    <  1:1051840/150‑1 (MQ=255)

                                                                                                    aaGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCg                                                   <  2:3096889/150‑1 (MQ=255)

                                                                                                     aGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTCTCgg                                                  >  2:3975359/1‑150 (MQ=255)

                                                                                                        cAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGTGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCt                                               <  1:421932/150‑1 (MQ=255)

                                                                                                        cAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCt                                               <  1:1601391/150‑1 (MQ=255)

                                                                                                         aGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTg                                              <  1:3431674/150‑1 (MQ=255)

                                                                                                          gCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGt                                             >  1:2514791/1‑150 (MQ=255)

                                                                                                            aaCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTCTCGGCCTGTaa                                           >  2:376868/1‑150 (MQ=255)

                                                                                                            aaCTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTaa                                           >  1:698552/1‑150 (MQ=255)

                                                                                                              ctgctgCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATc                                         <  1:3219496/150‑1 (MQ=255)

                                                                                                               tgctgcCGTTGGCTGCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCg                                        >  1:3661248/1‑150 (MQ=255)

                                                                                                                  tgcCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTg                                     <  2:2591063/150‑1 (MQ=255)

                                                                                                                    ccGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGAc                                   <  1:3804271/150‑1 (MQ=255)

                                                                                                                    ccGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGAc                                   <  1:1501589/150‑1 (MQ=255)

                                                                                                                     cGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACa                                  >  2:1598689/1‑150 (MQ=255)

                                                                                                                     cGTTGGCTGAAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAATATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACa                                  >  2:1598503/1‑150 (MQ=255)

                                                                                                                        tGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGATGACAGat                               >  2:1347499/1‑150 (MQ=255)

                                                                                                                         ggCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTCAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGata                              >  2:3539701/1‑150 (MQ=255)

                                                                                                                         ggCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGata                              <  1:1659541/150‑1 (MQ=255)

                                                                                                                             gCAACTGATGTAAACAGATCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCtt                          >  2:1901932/1‑150 (MQ=255)

                                                                                                                              cAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTc                         <  2:1966284/150‑1 (MQ=255)

                                                                                                                               aaCTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTcc                        <  2:4071586/150‑1 (MQ=255)

                                                                                                                                 cTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccac                      <  1:1422089/150‑1 (MQ=255)

                                                                                                                                   gATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTccacca                    <  1:1244919/150‑1 (MQ=255)

                                                                                                                                       tAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAg                >  1:3119292/1‑150 (MQ=255)

                                                                                                                                           cAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATg            <  1:4087345/150‑1 (MQ=255)

                                                                                                                                              aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtc         >  1:257349/1‑150 (MQ=255)

                                                                                                                                              aTCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtc         >  2:1526121/1‑150 (MQ=255)

                                                                                                                                               tCACATAATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtct        <  2:228847/150‑1 (MQ=255)

                                                                                                                                                cacaTCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTtctc       <  1:4027490/150‑1 (MQ=255)

                                                                                                                                                  catcatCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTcgc     >  1:3687988/1‑150 (MQ=255)

                                                                                                                                                   atcatcCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTcgcg    <  1:346519/150‑1 (MQ=255)

                                                                                                                                                     catcCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCt  <  1:1305865/150‑1 (MQ=255)

TTCGCTTTGTTGTAATGAGAGATAGCCCGCAGAACCGGCGTACCATTCTCGGGTGAACGGTTCGTGACGGGCGATAAACTGTCGCGCCAGATCGCGCGGTAAGCCAGCAACTGCTGCCGTTGGCTGCAACTGATGTAAACAGATCACATCATCCGCTTTGTTGAGTGAAGTCCAGATACAGCGGCGAAGATGCTGCACTTTACGCAGACGCAGTACCTGCGGCGGTAAAACATCCAGCGTCTGGGTATCGGCCTGTAATCGTTGACAGATATCTTCCACCACCAGCATGTTCTCGCGCT  >  CP009273/2372980‑2373278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`